황국균과 백국균의 대두 코지제조중 키토산의 효과

정순택,문길만,조건식,강성국,김설희 木浦大學校 工業技術硏究所 1998 工業技術硏究誌 Vol.8 No.-

The effect of chitosan in soybean koji preparation and characteristics of chitosan-koji were investigated. Aspergillus oryzae KCTC 6096 and Aspergillus kawachii KCCM 11458 was used for koji preparation. a-Amylase activity of soybean koji prepared with 0.25% 10cp chitosan was excellent relative to 30 cp chitosan and 1.6 times higher than non-added soybean koji. Acid and neutral protease activities of soybean koji prepared with 0.25%, 10 cp chitosan were showed simillary higher value than non-chitosna added koji. The period of koji preperation was reduced 30% by adding chitosan.

현훈증 감별에 있어 뇌간청각유발전위의 진단적 의의

조경원,정원영,나정균,임건한 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.1

Sudden onset vertigo is a common complaint in patients who are referred for neurological evaluation. But the entire physical examination and other diagnostic tests may be normal in these patients. Therefore we need more accurate diagnostic tools in the differential diagnosis between the peripheral and the contral vertigo. To evaluate diagnostic effectiveness, we performed Brainstem Auditory Evoked Potentials(BAEP) in the patients with 19 brainstem infarction, 23 peripheral vertigo, 20 vertebrobasilar transient ischemic attack(VB TIA) and analyzed the absolute latency of wave Ⅰ, Ⅲ, Ⅴ and interpeak latency of wave Ⅰ-Ⅲ, Ⅲ-Ⅴ,Ⅰ-Ⅴ compared to normal control data. The results were summerized as follows : 1. There was no significant differences in absolute and interpeak latencies of each BAEP waves between the group of normal control and the group of peripheral vertigo. 2. Among 19 VB TIA patients, prolonged absolute and interpeak latencies of BAEP waves were detected in eleven patients(57.9%) and in nine patients(47.3%), respectively. The abnormal findings were bilaterally in five patients(26.3%) 3. Among 20 brainstem infarction patients, ten patients(50%) showed abnormal BAEP findings, including prolonged interpeak latencies(in three, 15%) and absence of waveform(in seven, 35%). Therefore it is concluded that BAEP could be helpful in differential diagnosis of pathints with peripheral vertigo, V-B TlA, brainstem infarction and other disease of similiar symtomatology.

再生骨材 콘크리트의 特性에 미치는 잔골재 混合의 影響에 關한 實驗的 硏究

李正熙,宋河永,文鐘健,趙明元 大田産業大學校 1997 한밭대학교 논문집 Vol.14 No.1

It is becoming increasing1y difficult and expensive for construction contractors not only to dispose of building waste and demolition rubble but also to obtain natural aggregates of good quality for concrete product and building construction. As one of solutions for the above-mentioned problems, this study was planned to suggest a fundamental data and method for producing recycled aggregate concrete using crushed cement bricks as fine aggregates. In this study, one normal concrete with natural river sand as fine aggregate and crushed stone as coarse aggregate and three recycled aggregate concretes according to the mix proportion of recycled fine aggregate substitution ratio of 35%, 65%, 100% were made using tile water-cement ratio of 45% and 65%.

Electronic Structure Calculations of Silicon Using the Tight binding Method

강갑중,이근우,오영기,조화석 嶺南大學校附設 基礎科學硏究所 1985 基礎科學硏究 Vol.5 No.-

The local density of states of bulk Si is calculated by using the tight-binding method and the recursion method. The matrix elements of the Hamiltonian are obtained from the calculation using the exponentially decaying orbitals with an empirical pseudopotential Hamiltonian and the calculation using empirical tight-binding approaches.

동종 조혈모세포이식 후 발생한 치명적 홍역 폐렴 1예

백창렬,이동건,최정현,정현화,조유경,박훈준,이승훈,박윤희,이교영,민우성,김춘추,신완식 대한감염학회 2001 감염 Vol.33 No.4

As a result of the enlarging pool of unvaccinated children and young adults, there has been an increase in measles in our countries. In these situation, it has been reported that measles associated pneumoinia is easily complicated with fatal respiratory failure, espycially in immunocompromised patients. Herein we report the case of lethal measles pneumonia after allogenic hematopoietic stem cell transplantation in adults proven by autopsy. Recently, one case of measles was encountered in 39-year-old female patients after allogenic bone marrow transplanted case (chronic myelogenous leukemia), who progressed into interstitial pneumonia pattern, despite treatment including antibiotics, immunoglobulin. The patient died of giant cell pneumonia compatible with that of measles which was comfirmed in the section of necropsy lung specimen. (Korean J Infect Dis 33:301∼309, 2001)

조혈모세포이식 환자에서 침습성 진균 감염에 대한 Micafungin의 예방 효과 및 안전성

김시현,이동건,최수미,권재철,박선희,최정현,유진홍,이성은,조병식,김유진,이석,김희제,민창기,조석구,김동욱,이종욱,민우성,박종원 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Background: Micafungin, a potent inhibitor of 1,3-β-D-glucan synthase, is a novel antifungal agent of the echinocandin class. In vitro study showed that micafungin was effective against Aspergillus species as well as Candida species, but clinical data on the prophylactic efficacy against invasive fungal infections (IFIs) other than candidiasis are still lacking. Materials and Methods: We identified 60 consecutive adult hematopoietic stem cell transplantation (HSCT) recipients who received at least 3 doses of micafungin during neutropenic period. Micafungin was started as an alternative in patients who were intolerant or had adverse events (AEs) to primary prophylactic antifungal agents. We retrospectively reviewed the medical records and analyzed the efficacy and safety of micafungin for prophylaxis against IFIs. Results: The patients either had autologous (n=9) or allogeneic (n=51: 1 syngeneic, 24 sibling, 26 unrelated donor) HSCT. Itraconazole oral solution (n=58) was the most frequently used first line antifungal agent for prophylaxis and was administered for median 11 days. The most frequent cause of switch to micafungin was vomiting (n=42). The duration of neutropenia and micafungin administration was median 13 and 12 days, respectively. A successful outcome was achieved in 45 (75%) patients. Empirical antifungal therapy was initiated in 13 (22%) patients. There were 2 cases (3.3%) of breakthrough fungal infections which comprised a probable invasive pulmonary aspergillosis and a possible invasive fungal sinusitis. There was no case of invasive candidiasis. A total of 53 (88%) patients experienced at least one AE regardless of causality during micafungin administration. The most frequent AEs were hypokalemia, vomiting, diarrhea, and elevated serum aspartate aminotransferase or alanine aminotransferase. Among the aforementioned AEs, only 1 case of diarrhea could be classified as a probable relation with micafungin when causality was assessed. There was no AEs that caused discontinuation of micafungin. Conclusions: Micafungin seems to be a safe and effective agent for prophylaxis of IFIs including aspergillosis as well as candidiasis in HSCT recipients. However, further large, prospective, and randomized comparative studies are warranted for aspergillosis.

IMT-2000 환경에서 Block Design을 응용한 회의용 인증메카니즘의 설계

이여진,김중태,조영주,유성진,배용근 조선대학교 전자정보통신연구소 2003 電子情報通信硏究所論文誌 Vol.6 No.1

In this paper, we present conference key authentication mechanism by employing an algebraic method on IMT-2000 environment To accomplish this, Symmetric Balanced Incomplete block design is applied for generating the communication key and then this key is distributed to participants Through this technique for creating a conference key and mutual authentications performed based on identification information, the communication protocol is designed The protocol presented minimizes the transmission complexity for generating a conference key On a special case the time complexity is O(v√v), where v is the number of participant. The security of the mechanism, which is a significant problem in the construction of Secure system, can be, proved as computationally difficult to calculate as factoring and discrete logarithms.

동종 조혈모세포이식 후 발생한 크립토콕쿠스 척추염 1예

고윤호,임동준,이성수,조유경,이동건,최정현,김유진,민창기,김동욱,박정미,김춘추,신완식 대한감염학회 2001 감염 Vol.33 No.4

Skeletal cryptococcosis is an uncommon infection. Cryptococcus is a common cause of meningitis and infects 7∼10% of patients with AIDS. As well as AIDS, the infection may be seen in association with leukemia, lymphoma, Hodgkin's disease, sarcoidosis, tuberculosis and diabetes, also in patients on steroid medication. But there is no case report of skeletal cryptococcosis following allogeneic hematopoietic stem cell transplantation. A 40-year-old woman was admitted to the hospital because of low back pain. She had chronic myelogenous leukemia for 2 years and underwent allogeneic hematopoietic stem cell transplantation 8 months ago. She have been treated with steroid and cyclosporine orally because of chronic graft versus host disease. On examination she was afebrile and had posterior lower lumbar tenderness. But, she had no reduced strength of low extremities. Open biopsy was underwent. Histology demonstrated budding, round-to-oval, refractile yeast-like organisms within debris. The results of a lumbar puncture were unremarkable and cerebrospinal fluid culture failed to grow bacteria and yeast. The patient was treated with amphotericin B (1 gram) and AmBisome□ (2.8 gram) over 6 weeks. Three months after cessation of therapy, the patient was doing well.(Korean J Infect Dis 33:298∼301, 2001)

Climate Cues of Alternate Bearing in Commercial Apple Orchards Across South Korea

Jung Gun Cho,Jae Hoon Jeong,Suhyun Ryu,Jeom Hwa Han,Hyun Hee Han,Kyeong Ran Do 한국원예학회 2018 한국원예학회 학술발표요지 Vol.2018 No.10

High-glutathione mesenchymal stem cells isolated using the FreSHtracer probe enhance cartilage regeneration in a rabbit chondral defect model

Gun Hee Cho,Bae Hyun Cheol,조원영,정의만,Hee Jung Park,Ha Ru Yang,Sun Young Wang,You Jung Kim,Shin Dong-Myung,Hyung-Min Chung,In Gyu Kim,한혁수 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Mesenchymal stem cells (MSCs) are a promising cell source for cartilage regeneration. However, the function of MSC can vary according to cell culture conditions, donor age, and heterogeneity of the MSC population, resulting in unregulated MSC quality control. To overcome these limitations, we previously developed a fluorescent real-time thiol tracer (FreSHtracer) that monitors cellular levels of glutathione (GSH), which are known to be closely associated with stem cell function. In this study, we investigated whether using FreSHtracer could selectively separate high-functioning MSCs based on GSH levels and evaluated the chondrogenic potential of MSCs with high GSH levels to repair cartilage defects in vivo. Methods Flow cytometry was conducted on FreSHtracer-loaded MSCs to select cells according to their GSH levels. To determine the function of FreSHtracer-isolated MSCs, mRNA expression, migration, and CFU assays were conducted. The MSCs underwent chondrogenic differentiation, followed by analysis of chondrogenic-related gene expression. For in vivo assessment, MSCs with different cellular GSH levels or cell culture densities were injected in a rabbit chondral defect model, followed by histological analysis of cartilage-regenerated defect sites. Results FreSHtracer successfully isolated MSCs according to GSH levels. MSCs with high cellular GSH levels showed enhanced MSC function, including stem cell marker mRNA expression, migration, CFU, and oxidant resistance. Regardless of the stem cell tissue source, FreSHtracer selectively isolated MSCs with high GSH levels and high functionality. The in vitro chondrogenic potential was the highest in pellets generated by MSCs with high GSH levels, with increased ECM formation and chondrogenic marker expression. Furthermore, the MSCs’ function was dependent on cell culture conditions, with relatively higher cell culture densities resulting in higher GSH levels. In vivo, improved cartilage repair was achieved by articular injection of MSCs with high levels of cellular GSH and MSCs cultured under high-density conditions, as confirmed by Collagen type 2 IHC, Safranin-O staining and O’Driscoll scores showing that more hyaline cartilage was formed on the defects. Conclusion FreSHtracer selectively isolates highly functional MSCs that have enhanced in vitro chondrogenesis and in vivo hyaline cartilage regeneration, which can ultimately overcome the current limitations of MSC therapy.