조직 배양된 실험쥐 해마부위 신경원의 전기적 발작 손상에 대한 발열의 영향

채수안(Soo Ahn Chae),박용민(Yong Min Park),유병훈(Byoung Hoon Yoo),김동욱(Dong Wook Kim) 대한소아신경학회 2002 대한소아신경학회지 Vol.10 No.2

목 적 : 간질환자들은 고열 발생시 발작의 발현이 증가하는 경향이 있다. 이것은 간질상태의 뇌에서 고체온이 신경원의 과흥분성 및 손상과 관련이 있음을 시사하는 것이다. 따라서 본 저자들은 실험쥐 해마부위 조직배양을 이용하여 신경원의 발작 손상에 대한 발열의 영향을 알아보고자 하였다. 방 법 : 실험쥐 해마부위 조직배양을 aCSF의 온도를 39℃까지 단순히 상승시킨 단순 발열군과 aCSF 온도의 상승 없이 36℃에서 조직을 유지시킨 단순 비발열군으로 분류하여 발열이 정상 신경원에 손상을 유발시키는지의 여부와 처음에 36℃에서 stimulus train을 가한 후 aCSF의 온도를 39℃까지 상승시킨 후 2번째 stimulus train을 가한 발열 발작군과 2번째 stimulus train을 가할 시에도 온도를 36℃로 유지시킨 비발열 발작군에서 발열중의 발작과 비발열중의 발작시 신경원 손상의 차이가 있는지를 알아보았다. 결 과 : 발열이 정상 신경원에 손상을 유발시키는지의 여부를 알아보기 위한 단순 발열군과 단순 비발열군 간의 신경원 손상정도 비교에서 해마의 CA1과 CA3 부위 모두에서 실험 24, 48, 72시간 경과 후 두 군간의 통계적으로 의미 있는 신경원 손상의 차이를 발견할 수 없었다. 발열증의 발작과 비발열증의 발작시 신경원 손상의 차이가 있는지를 알아보기 위한 발열 발작군과 비발열 발작군의 신경원 손상정도의 비교에서 해마의 CA1 부위이 션경원 손상정도는 AD 유발 후 24, 48, 72시간 경과 후 모두에서 발열 발작군이 비발열 발작군에 비하여 신경원 손상이 통계적으로 의미 있게 많았고, CA3 부위의 신경원 손상 정도는 AD 유발 후 24시간과 48시간 경과 후에서는 두 군간에 통계적으로 의미 있는 신경원 손상의 차이가 없었지만 72시간 경과 후에서는 발열 발작군이 비발열 발작군에 비하여 통계적으로 의미 있게 신경원 손상의 정도가 심하였다. 결 론 : 정상 뇌에서 단순 고체온은 신경원 손상을 유발시키지 않으나 간질상태의 뇌에서 고체온시의 발작은 정상체온에서의 발작시보다 신경원 손상을 심화시킬 수 있음을 시사하는 것이다. 이것은 간질 상태의 뇌에서는 발열이 발작으로 인한 뇌손상에 상승 효과를 유발시킬 수 있으므로 간질 환자에서 발열 발생 시 신속하고도 적절한 처치가 발열로 인한 발작으로 초래될 수 있는 뇌 손상을 예방하는데 중요할 것으로 생각된다. Purpose : Epileptic patients have a increasing tendency to develop seizure attack in high temperature. This finding suggests that high temperature may have an effect on neuronal hyperexcitability and injury of epileptic brain. Therefore, the influence of high temperature on normal and epileptic brain was studied in organotypic explant cultures of rat. Methods : Fourteen days-in-vitro cultures from 8 day-olf rat pups were perfused with standard aCSF bubbled with 95%/5% O/CO in a microchamber. Stimulus train(0.3 sec, 60 Hz) was applied to Schaffer collaterals in CA3 and extracellular field potential was recorded in the CA1 pyramidal layer. At 36℃ initially, AD was evoked. In high temperature(HT) group, the cultures were subjected to 39℃ for a period of 8 min before the second stimulus train was applied. They were then restored to 36℃ for 10 min. In normal temperature group, temperature was maintained at 36℃ for the second stimulus train. The cultures were returned to the incubator and observed serially for neuronal damage. Intensity of propidium iodide fluorescence indicative of neuronal injury was quantitated by digital image analysis. The cultures on the same insert that were not stimulated served as the unstimulated groups. Results : There was not a statistically significant difference in neuronal damage between the unstimulated high-temperature(HT) and normal-temperature(NT) group. In CA1 sector, % damage(mean±SEM) was 0.42±0.20 vs 0.27±0.05 at 24 hrs(HT vs NT)group, n=16 each, P>0.05, Student t-test); 1.81±0.79 vs 1.43±0.27 at 48 hrs(P>0.05); 3.50±1.32 vs 3.35±0.56 at 72 hrs(P<0.05). In CA3 sector, % damage was 0.34±0.10 vs 0.20±0.03 at 24 hra(P>0.05); 0.99±0.20 vs 0.83±0.23 at 48 hrs(P>0.05); 2.00±0.38% vs 2.26±0.35% at 72 hrs(P>0.05). Neuronal damage on AD induced cultures during febrile setting(n=16) was significantly higher than in nonfebrile setting(n=16). In CA1 sector, % damage was 6.63±2.56 vs 0.92±0.45 at 24 hrs(febrile setting vs nonfebrile setting, P=0.036); 26.37±7.44 vs 4.99±2.23 at 48 hrs(P=0.010); 38.59±9.63 vs 6.48±2.30 at 72 hrs (P=0.003). In CA3 sector, % damage was 1.23±0.48 vs 3.91±2.37 at 24 hrs(P=0.277); 13.09±5.75 vs 5.93±3.27 at 48 hrs(P=0.288); 27.86±8.68 vs 7.54±3.74 at 72 hrs(P=0.04). Conclusion : At high temperature, seizures in epileptic brain may be more injurious than seizures in normal temperature.

C -11 및 F - 18 표지 콜린의 합성과 체내동태에 관한 연구

전권수,유국현,김상욱,임상무,홍성운,서용섭,양승대,안순혁,허민구 대한핵의학회 2001 핵의학 분자영상 Vol.35 No.3

Objectives: Recently, [methyl-(11)^C]-(β-Hydroxyethyl)trimethylammonium ([(11)^C]choline) has been discovered to be a very effective tracer in imaging various human tumors using positron emission tomography. Because of the short half-life of C-11, it is very difficult to use in a routine imaging procedure and needs a frequent synthesis of [(11)^]choline. This can be supplemented by the substitution of [(11)^Ccholine with [methyS-18]fluorocholine. Here, we would like to report cell uptake and biodistribution of [(11)^Ccholine and [(18)^F]fluorocholine as a basic study. Methods [(11)^C]Choline was prepared by the treatment of [(11)^C]CHzI with N,N-dimethylaminoethanol and [18F]fluorocholine was synthesized from reaction of CHzBr[18F]F with N,N-dimethylaminoethanol. The radiochemical purity was checked by high performance liquid chromatography (HPLC). The biodistribution of [(11)^C]choline and [(18)^F]fluorocholine was determined in balb/c mouse at 5 min, 20 min, 40 min and 80 min. The cell uptake wa measured using glioma (9L) and colon adeocarcinoma (SW620). Results: The radiochemical purity was more than 98% after purification. In the liver, uptake did not change over time the uptake was 20/ID/g for [C]choline and 13%ID/g for [(18)^F]fluorocholine. In the kidney, radioactivity decreased over tirne the uptake was 15%1D/g for [(11)^Ccholine and 20%ID/g for [(18)^F]fluorocholine, 80 min post-injection. The cell uptake of [(11)^Ccholine was 4.93% for glioma (9L) and 18.69F for colon adenocarcinoma (SW620). For [(18)^F]fluorocholine, 1.77% for glioma (9L) and 2.77% for colon adenocarcinoma (SW620). Conclusion: [(11)^CCholine and [(18)^F]fluorocholine showed a different cell uptake tendency, depending on cancer cell line. (Korean J Nucl Med 200135:185-191)

위상최적화를 이용한 트레일러의 경량설계

안서욱,이신영,윤민수,장강원 한국공작기계학회 2009 한국공작기계학회 춘계학술대회논문집 Vol.2009 No.-

A new design for a lightweight flatbed trailer with high bending and torsional stiffness is presented by using an integrated CAE-based optimization procedure. The integrated design procedure consists of two main steps: topology optimization and thickness optimization. During topology optimization, a creative frame layout different from existing ladder-type frames can be obtained by searching the best layout out of all possible layouts of a simplified design domain model. After approximating the result of topology optimization as a thin-walled structure, the approximated thicknesses of the plates are optimized to minimize the mass of a trailer.

혈액투석중인 만성신부전 환자에서 골대사 지표로써의 Osteocalcin치

송치운,이진홍,안미애,윤환중,윤상임,성기양,이강현,송민호,이강욱,신영태,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

Background : Serum osteocalcin is synthesized by osteoblast and has been shown to be sensitive indicator of bone turnover inpatients with various metabolic bone disease. In renal osteodystrophy, serum osteocalcin is elevated due to decreased renal clearance and elevated level of PTH. This study was done to evaluate the usefulness of serum osteocalcin as a marker of bone metabolism and the correlation with other biochemical markers of bone metabolism. Methods : We measured serum osteocalcin, calcium, phosphorus, ALP(alkaline phosphatase) and PTH(parathyroid hormone) in 37 patients with end stage renal disease on hemodialysis. Osteocalcin was determined by radioimmunoassay and PTH was determined by radioimmunometric assay. Results : 1) The mean level of serum osteocalcin in ESRD patients was 233.8± 218.2ng/ml which was significantly higher than that of controls(p<0.0001). 2) The mean level of serum PTH in ESRD patients was 40.5± 43.8pg/ml was significantly higher than that of controls(p<0.005). 3) There was a significant positive correlation between the level of serum PTH, ALP and the level of serum osteocalcin in ESRD patients. 4) By using multiple regression, PTH is most reliable factor that affect to elevated level of serum osteocalcin ( beta coefficient = 0.687, Sig T<0.05). Conclusion : Serum osteocalcin as a marker of bone metabolism in ESRD patients is more useful than other biochemical marker such as serum calcium, phosphorus, ALP and PTH is a most reliable factor that affect to elevated level of serum osteocalin.

당뇨병환자에서 게이트심장혈액풀신티그라피를 이용한 심기능 평가

윤상임,송치운,이진홍,안미애,성기양,송민호,이강욱,신영태,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

Major cardiovascular complications of diabetes are coronary atherosclerosis, diabetic dilated cardiomyopathy, autonomic neuropathy and those are major causes of morbidity and mortality in diabetic patients. Gated blood pool heart scan is noninvasive and useful method for evaluation of functional status of heart in diabetics. We evaluated 52 patients with diabetes and divided 3 groups. Group 1 were 11 patients without proteinuria or with proteinuria less than 550mng during 24 hours. Group 2 were 9 patients with proteinuria more than 550mg during 24 hours and group 3 were 32 patients with endstage renal diasese due to diabetes. We performed 99mTc-HSA cardiac gated blood pool scan and used left ventricular ejection fraction(LVEF), peak ejection rate(PEF) to indices of LV systolic function and peak filling rate(PER) to index of LV diastolic function. The results were follows : 1) LVEF, PER were significantly lower in diabetics with ESRD than diabetics without ESRD, but there were no significant difference between normal controls and diabetics without ESRD 2) PFR was significantly lower in diabetics than normal controls, but there were no significant differences in diabetics with or without nephropathy. 3) There were negative correlation between PER, PFR and duration of diabetes. On the basis of results, PFR is a LV functional index of GBPS which can disclose early change of LV dysfunction in patients with diabetes.

정상상피세포(HaCat)와 자궁경부 암세포(SiHa)에서 GeneFishing^(TM) PCR technique을 이용한 유전자 발현의 변화

김병훈,배수미,서민제,김용완,이정웅,김용욱,이준모,남궁성은,김종국,안웅식 대한부인종양 콜포스코피학회 2003 Journal of Gynecologic Oncology Vol.14 No.4

목적 : 본 실험의 목적은 정상상피 세포와 자궁경부 암세포 사이에서 유전자의 발현 차이를 조사하였다. 연구 방법 : 정상상피 세포(HaCat)와 자궁암 세포(SiHa)를 사용하였으며, 두 세포 간에 유전자 발현 차이를 GeneFish^(TM) PCR을 이용하여 알아보았으며, BLAST serach를 통해 분석하였다. 결과 : 정상상피 세포와 자궁암 세포 비교 결과, 자궁암 세포에서 S1-2-2와 S5-1을 포함한 25개의 유전자가 발현이 증가하였고, 24개의 유전자가 감소하였다. 결론 : GeneFishing^(TM) PCR기법은 유전자의 발현 변화를 확인하는데 있어서 아주 민감하고 효과적인 방법이다. 우리는 정상상피 세포와 자궁경부 암세포에서 다르게 발현하는 유전자를 찾을 수 있었고, 앞으로는, 종양의 발생과 진행과정에 관여하는 유전자를 더 탐지하고 해당 유전자의 기능을 연구할 필요가 있다고 생각된다. Objective : The purpose of this study is investigated the differentially expressed genes between normal and cervical cancer cell line. Methods : We used normal human keratinocyte (HaCaT) as a control and HPV-16 positive cervical cancer (SiHa) cell line. Two cell lines were studied differential expressed genes by using GeneFishing^(TM) PCR and analyzed with BLAST search. Results : As compared with normal, cervical cancer cell line was showed 25 up-regulated genes including the S1-2-2, S5-1 and 24 down-regulated genes. Conclusion : GeneFish^(TM) PCR test is very sensitive and effective method for detection of changed gene expression. We could search differentially expressed genes between normal and cervical cancer cell line. In the future, we need to research various genes function to participate in the process of tumor development and progression.

Development of the Korean Version of the Gastrointestinal Quality of Life Index Questionnaire

( In Jun Yang ),( Heung-kwon Oh ),( Jeehye Lee ),( Jung Wook Suh ),( Hong-min Ahn ),( Hyeonjeong Park ),( Hyun Hee Sim ),( Yong Beom Cho ),( In Kyu Lee ),( Seungbum Ryoo ),( Dong-won Lee ),( Duck-woo 한국정맥경장영양학회 2022 한국정맥경장영양학회지 Vol.14 No.1

Purpose: To establish a standardized quality of life measurement that allows global cross-study comparisons, we translated the Gastrointestinal Quality of Life Index (GIQLI) into Korean and linguistically validated the Korean version of the GIQLI (K-GIQLI) in patients who underwent colorectal surgery. Materials and Methods: A cross-cultural adaptation of the original GIQLI was created based on the established guidelines. Based on participation in a cognitive interview, 20 patients with colorectal cancer were enrolled in the study. To ensure that the Korean version of the questionnaire was understood as intended, the time needed to complete the questionnaire was measured, and three additional items related to comprehension were added. Results: From May to July 2021, two translators, whose native language was Korean translated the GIQLI items into Korean, and a native English editor who had no knowledge of the original questionnaire translated the items back into English. In the cognitive interview, the median age of the patients was 61.8 (range: 44~82) years, and the median time required to complete the questionnaire was 6.5 (range: 5~10) min. For the language and cultural adaptation process, the participants’ comprehension of the questionnaire was measured on a scale of 1~5, with a mean score of 4 (range: 3~4). Conclusion: The K-GIQLI was developed and did not exhibit a significant difference from the original English version in terms of social, linguistic, and cultural differences between the Western world and Republic of Korea.

A Study on the Design of the Stabilizer of Coated Conductor for Applying to SFCL

Min Cheol Ahn,Dong Keun Park,Seong Eun Yang,Min Jae Kim,Ho Min Kim,Hyoungku Kang,Kwanwoo Nam,Bok-Yeol Seok,Jung-Wook Park,Tae Kuk Ko IEEE 2007 IEEE transactions on applied superconductivity Vol.17 No.2

<P>A superconducting fault current limiter (SFCL) using high temperature superconducting (HTS) wire has been developed in Korea. While the first-generation wire has difficulty in adopting various matrices because it is made by PIT process, the second-generation wire has flexibility in the design of its stabilizer. In general, coated conductor (CC) tape is clad with a thick metal layer as a stabilizer on the superconducting layer such as YBCO. In addition, the metal layer has good electrical conductivity. However, resistive SFCL, when a fault occurs, limits fault current using high resistance of the stabilizer. Therefore, the design of stabilizer is one of the most important parts in the design of SFCL. In this paper, over-current characteristics of CC tapes clad with stabilizers having various specifications were experimentally investigated. It was possible to design the stabilizer of CC based on calculation. An SFCL rated on 13.2 kV/630 A was designed using the optimal design of the stabilizer.</P>

Recovery Characteristics of Resistive SFCL Wound With YBCO Coated Conductor in a Power System

Min Cheol Ahn,Dong Keun Park,Seong Eun Yang,Min Jae Kim,Ho-Myung Chang,Yong Soo Yoon,Bok-Yeol Seok,Jung-Wook Park,Tae Kuk Ko Institute of Electrical and Electronics Engineers 2007 IEEE transactions on applied superconductivity Vol.17 No.2

<P>Since resistive superconducting fault current limiters (SFCLs) are inserted to a power system directly, it is necessary to recover instantly after the clearance of a fault. Most resistive SFCLs using BSCCO bulk or thick film have a recovery time of tens of seconds. An SFCL using YBCO coated conductor (CC) has a large surface area contacted to liquid nitrogen. Joule heat flux of the SFCL is smaller than that of other types when a fault occurs. Therefore, it is important for the SFCL employing CC to investigate a recovery time. In this paper, the recovery characteristics of SFCL with respect to applied voltage were analysed in a power system. All tests were performed in liquid nitrogen and sub-cooled nitrogen. From this result, the parameters for recovery time were obtained.</P>

피부색과 GMM 배경 분리를 이용한 가상 터치스크린

김민욱 ( Min-wook Kim ),오치민 ( Chi-min Oh ),( Dhi Aurrahman ),안양근 ( Yang-keun Ahn ),이칠우 ( Chil-woo Lee ) 한국정보처리학회 2008 한국정보처리학회 학술대회논문집 Vol.15 No.1

일반적인 터치스크린은 물리적인 막에 의존해야 한다는 점과 크기에 제약을 받는 다는 단점이 있고, 기존에 제시되있는 스테레오 기술만을 이용한 가상 터치스크린은 스크린 상에는 손 외에 움직이는 물체가 없다고 가정하는 제약이 있다. 하지만, 기존의 터치 스크린과 같이 가상막을 이용하면서도, 배경 분리 기술과 피부색 추출 기술을 사용해서 사람의 신체 중에 피부색을 띄는 부분을 추출하고, 스테레오 기술에서 얻어진 깊이 값을 이용하여 가상막 형성하면, 가상막을 통과하는 사람의 손이나 얼굴 등 신체의 특정 부분을 추출 할 수 있다. 이 기술은 기존에 제안되었던 가상 터치 기술보다 더 안정감 있고, 고감도의 성능으로 터치를 인식 할 수 있어서 게임을 비롯한 여러 어플리케이션에 적용이 가능하다.