한국인 소아에서 분리한 Helicobacter pylori 균주의 항균제 내성률

김유미,이연주,오석희,성흥섭,김미나,김경모,Kim, Yoo-Mi,Lee, Yeoun-Joo,Oh, Seak-Hee,Sung, Heung-Sup,Kim, Mi-Na,Kim, Kyung-Mo 대한소아소화기영양학회 2011 Pediatric gastroenterology, hepatology & nutrition Vol.14 No.1

목 적: Helicobacter pylori (H. pylori) 제균 치료의 적절한 항균제 선택의 기반이 되기 위해 소아에서 H. pylori 항균제 내성률을 알아보고자 하였다. 방 법: 2003년 7월부터 2009년 4월까지 상복부 통증으로 본원을 방문한 환자 중 위 문부 생검 조직 배양에서 H. pylori 가 동정된 균주를 대상으로 항균제 감수성 검사를 시행하였다. 감수성 검사는 clarithromycin과 amoxicillin은 디스크 확산법으로, tetracycline과 metronidazole은 E-test를 시행하였다. 치료 원칙은 감수성 결과가 확인 된 환자는 감수성 결과에 따라 항균제를 선택 하였고, BAM (bismuth subsalicylate, amoxicillin, metronidazole) 또는 OAC (omeprazole, amoxicillin, clarithromycin)를 바탕으로 한 경험적 삼제요법을 택하였다. 치료 실패 시에는 bismuth (또는 bismuth subsalicylate)를 포함한 사제요법을 선택하였다. 결 과: 33명의 환자가 배양검사 양성을 보였고 28명의 환자에서 감수성 검사를 시행하였다. 이 중 항균제 내성을 보인 환아는 9명(32.1%)으로 그 중 7명(25%)이 clarithromycin 내성 균주를 보였고 5명(17.8%)이 metronidazole 내성 균주를 보였다. 대상이 적어 통계적인 분석을 하지 못하였으나 Clarithromycin과 metronidazole의 내성률이 시기적으로 감소하는 추세를 보였다. 결 론: 본 연구는 단일 병원에서 소수의 환아를 대상으로 이루어진 연구로 한계가 있으나, 간접적으로 국내소아 환아에서 분리된 H. pylori의 균주에서도 높은 항균제 내성과 시기적으로 내성률이 변하는 것을 알 수있었다. 따라서 소아 환자에서 적절한 항균제 선택을 제시하기 위한 보다 광범위한 연구가 필요한 것으로 생각한다. Purpose: The antimicrobial resistance of Helicobacter pylori is one of the critical factors in failure of eradication therapy. The purpose of this study was to evaluate antimicrobial resistance of H. pylori in Korean children. Methods: Gastric mucosal specimens for H. pylori were obtained from children with dyspepsia who were cared for at Asan Medical Center Children's Hospital in Seoul, Korea between 2003 and 2009. Antimicrobial resistance tests were performed using the disk diffusion method for clarithromycin and amoxicillin and the E-test for metronidazole and tetracycline. Most children with H. pylori infections were treated using triple therapies. Results: Thirty-three children had positive H. pylori cultures, although a resistance test was only performed in 28 patients. Resistant strains were found in 9 children (32.1%). The resistance rates to clarithromycin and metronidazole were 25% and 17.8%, respectively. There was no resistance to amoxicillin or tetracycline. The resistance rates decreased from 44.4% (2003~2006) to 26.3% (2006~2009) during the study period. Conclusion: Korean children demonstrated relatively high antimicrobial resistance to H. pylori in this study. However, there was a temporarily decreasing trend during the study period. A larger multi-regional study may be needed to determine the optimal antimicrobial treatment for pediatric patients infected with H. pylori.

인터넷 정보조사를 통한 국내 환자용 식품의 판매가격과 영양 함량 평가

김유미,서유리,김미현,최미경,Kim, Yu-Mi,Seo, Yu-Lee,Kim, Mi-Hyun,Choi, Mi-Kyeong 한국식품영양학회 2021 韓國食品營養學會誌 Vol.34 No.1

The purpose of this study was to examine the relationship between the regular selling price and nutrient contents of foods used for special medical purposes in Korea. This study investigated the regular selling price and nutritional composition of 114 enteral nutrition (EN) foods on the manufacturer's internet homepage. The average price of the total products was 1,156.0 won/100 mL. The price of foods used for calorie and nutrient supplementation (CNS) was significantly higher compared to that of the other EN food types (p<0.01). With respect to the nutritional content per 100 mL of the product, EN foods for CNS had significantly higher contents of energy, protein, 5 minerals, and 7 vitamins than the other EN food types. On comparing the nutritional contents of foods according to the price, the balanced nutrition foods showed significantly higher contents of energy, carbohydrate, protein, 3 minerals, and 7 vitamins in high-priced products than in low-priced foods. Summarizing these results, foods for CNS were approximately twice as expensive as the other EN foods, and the energy, protein, mineral, and vitamin contents were also high. Balanced nutrition foods had higher nutrient contents in high-priced products, which showed the relationship between the product price and nutrient content.

한국 건강불평등의 현황과 문제점

김유미,김명희,Kim, Yu-Mi,Kim, Myoung-Hee 대한예방의학회 2007 예방의학회지 Vol.40 No.6

Objectives : The aim of this study is to summarize the current conditions and implications of health inequalities in South Korea. Methods : Through a literature review of empirical studies and supplementary analysis of the data presented in the 1998, 2001, and 2005 KNHANEs, we evaluated the extent and trends of socioeconomic inequalities in both health risk factors, such as smoking, physical activity, and obesity, and outcomes, such as total mortality, subjective poor health status by self-reports and metabolic syndrome. Relative risks and odds ratios were used to measure differences across socioeconomic groups, and the relative index of inequality was used to evaluate the changes in inequalities over time. Results : We found clear inequalities to various degrees?in most health indicators. While little change was observed in mortality differences over time, the socioeconomic gaps in risk factors and morbidity have been widening, with much larger differences among the younger population. Conclusions : Socioeconomic inequalities are pervasive across various health indicators, and some of them are increasing. The trends in socioeconomic inequalities in health should be carefully monitored, and comprehensive measures to alleviate health inequalities are needed, especially for young populations.

한국판 보건의료계열 직업 선택 및 간호사 직업 선택 척도의 타당화 연구: 국내 보건전공 대학생을 대상으로

김선경Kim,Sun Kyung,김선애Kim,Sun Ae,김유미Kim,Yu Mi 학습자중심교과교육학회 2019 학습자중심교과교육연구 Vol.19 No.23

본 연구는 보건의료계열 전공 학생들과 간호학 전공 학생들의 직업 선택에 영향을 미치는 요인을 알아내고자 개발된 Health career choice and nursing career choice (HCC-NCC)도구를 한국어판으로 번역 후 타당도와 신뢰도를 알아보고자 시 행되었다. 2019년 5월부터 9월까지 4개 지역 6개 대학의 450명의 보건의료계열과 간호학 전공 학생이 연구에 참여하였다. SPSS와 AMOS를 활용 수집된 자료로 문항분석, 탐색적 요인분석, 확인적 요인분석 및 기존의 희망직업 척도와 간호사 이미지 도구와의 동시타당도가 분석되었다. 문항분석과 탐색적 요인분석 결과 5요인 21문항의 한국어판 HCC-NCC도구가 도출되었고 확인적 요인분석을 통한 모델의 적정성이검증 되었다. 기존의 희망직업 척도와 간호사 이미지 도구와의 유의한 상관관계가 확인되었다. 한국어판 HCC-NCC도구의 활용을 통해 다른 보건의료계열과 구분되는 각 전공의 직업선택 요인에 대한 의미있는 근거를 제시 할 것이다. 또한 다른 보건의료계열 전공과 구분되는 간호학의 긍정적인 이미지를 활용 간호학 전공 신입생 유치에 적극 활용 가능 할 것이다. The purpose of this study was to investigate the validity and reliability of the Health Care Choice and Nursing Career Choice (HCC-NCC) tool, which was developed to find out the factors that influence the career choice of health care majors and nursing students. Data were collected from May to September 2017. Study participants were 450 healthcare and nursing students from 6 universities located in 4 districts in Korea. Data were analyzed by exploratory factor analysis, confirmatory factor analysis, validity of the existing Select Job Scale and Nurse Image scale by using SPSS and AMOS. As a result of item analysis and exploratory factor analysis, the Korean version of the HCC-NCC tool of 5 factors and 21 items was derived, and the adequacy of the model was verified through confirmatory factor analysis. Significant correlation was confirmed between the existing Hope Job Scale and the Nurse Image scalel. The use of the Korean version of the HCC-NCC tool will provide a meaningful basis for the factors of career choice in each major, as distinguished from other health care. In addition, the positive image of nursing science distinguished from other healthcare majors will be used to attract new students in nursing majors.

A neonate with hyperornithinemia-hyperammonemia-homocitrullinuria syndrome from a consanguineous Pakistani family

김유미,임한혁,Mi Hyeon Gang,Yong Wook Lee,Sook Za Kim,Gu-Hwan Kim,Han-Wook Yoo,Jung-Min Ko,Meayoung Chang 대한의학유전학회 2019 대한의학유전학회지 Vol.16 No.2

Hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome is a rare autosomal recessive urea cycle disorder. HHH is caused by a deficiency of the mitochondrial ornithine transporter protein, which is encoded by the solute carrier family 25, member 15 (SLC25A15) gene. Recently, government supported Korean newborn screening has been expanded to include a tandem mass spectrometry (MS/MS) measurement of ornithine level. We report a case of a neonate with HHH syn-drome showing a normal MS/MS measurement of ornithine level. A female newborn was admitted to neonatal intensive unit due to familial history of HHH syndrome. Her parents were consanguineous Parkistani couple. The subject’s older sister was diagnosed with HHH syndrome at age of 30 months based on altered mental status and liver dysfunction. Even though the subject displayed normal ammonia and ornithine levels based on MS/MS analysis, a molecular test conἀrmed the diagnosis of HHH syndrome. At 1 month of age, amino acid analysis of blood and urine showed high levels of ornithine and homoc-itrulline. After 11 months of follow up, she showed normal growth and development, whereas affected sister showed progres-sive cognitive impairment despite no further hyperammonemia after protein restriction and standard therapy. Our report is in agreement with a previous Canadian study, which showed that neonatal samples from HHH syndrome patients demonstrate normal ornithine levels despite having known mutations. Considering the delayed rise of ornithine in affected patients, ge-netic testing, and repetitive metabolic testing is needed to prevent patient loss in high risk patients.

저온 다결정 실리콘 박막 트랜지스터의 비정상적인 Hump 현상 분석

김유미,정광석,윤호진,양승동,이상율,이희덕,이가원,Kim, Yu-Mi,Jeong, Kwang-Seok,Yun, Ho-Jin,Yang, Seung-Dong,Lee, Sang-Youl,Lee, Hi-Deok,Lee, Ga-Won 한국전기전자재료학회 2011 전기전자재료학회논문지 Vol.24 No.11

In this paper, we investigated an anomalous hump phenomenon under the positive bias stress in p-type LTPS TFTs. The devices with inferior electrical performance also show larger hump phenomenon. which can be explained by the sub-channel induced from trapped electrons under thinner gate oxide region. We can confirm that the devices with larger hump have larger interface trap density ($D_{it}$) and grain boundary trap density ($N_{trap}$) extracted by low-high frequency capacitance method and Levinson-Proano method, respectively. From the C-V with I-V transfer characteristics, the trapped electrons causing hump seem to be generated particularly from the S/D and gate overlapped region. Based on these analysis, the major cause of an anomalous hump phenomenon under the positive bias stress in p-type poly-Si TFTs is explained by the GIDL occurring in the S/D and gate overlapped region and the traps existing in the channel edge region where the gate oxide becomes thinner, which can be inferred by the fact that the magnitude of the hump is dependent on the average trap densities.

고셔병 환자에서 효소대체치료의 장기적 효과와 한계

김유미,Kim, Yoo-Mi 대한유전성대사질환학회 2014 대한유전성대사질환학회지 Vol.14 No.1

Gaucher disease is a multisystemic disorder arising from a deficient activity of the lysosomal enzyme glucocerebrosidase, which leads to accumulation of glycosylceraide and other glycolipids in the regiculoendothelial system. The characteristics of Gaucher disease are anemia, thrombocytopenia, hepatosplenomegaly, and skeletal disease. Enzyme replacement therapy (ERT) has been proven to prevent progressive manifestations of Gaucher disease and effective in improving anemia, thrombocytopenia, bone markers and biomarkers. However, some patient needs still remain unmet because of the inaccessibility of certain sites including brain, bone and various organs. ERT could not Improve the irreversible lesion such as liver fibrosis, hepatopulmonary syndrome, and necrosis or infarction of bone and other organs. Adult patients with Gaucher disease should be screened for longterm complication such as bone disease, pulmonary hypertension, gallstone, and cancer, especially in patients with splenectomy. Parkinsonism and polyneuropathy was also reported among patients with type 1 Gaucher disease, but ERT does not improve neurological function. We need to review the benefits and unmet needs of ERT in Gaucher disease.

GPS 서비스

김유미,Kim, Yu-Mi 한국통신학회 2003 정보와 통신 Vol.20 No.4

KURT 지하심부 지하수 내 토착 금속환원미생물의 종 다양성 및 철/망간의 환원과 생광물화작용

김유미,오종민,정혜연,이승엽,노열,Kim, Yumi,Oh, Jong-Min,Jung, Hea-Yeon,Lee, Seung Yeop,Roh, Yul 대한자원환경지질학회 2014 자원환경지질 Vol.47 No.4

이 연구의 목적은 KURT(KAERI underground research tunnel) 지하수 내에 금속이온을 환원시키는 미생물의 존재 여부를 확인하고 배양하여, 이들의 활동에 따른 철과 망간 환원의 관찰과 환원물의 광물학적 특성을 연구함으로써, 금속환원미생물에 의한 산화상태로 존재하는 철과 망간의 환원과 광물 상전이 가능성을 확인하는 것이다. KURT 지하수 내 금속을 환원하는 미생물은 전자공여체로 포도당, 초산, 젖산, 개미산, 피루브산을, 전자수용체로 Fe(III)-citrate를 사용하여 농화배양 하였으며, 16S rRNA 분석을 통해 종 다양성을 확인하였다. 농화배양된 금속환원미생물에 의한 철과 망간의 환원과 생광물화작용을 알아보기 위해 전자공여체로 포도당, 초산, 젖산, 개미산, 피루브산을, 전자수용체로 철수산화물인 아카가나이트(akaganeite, ${\beta}$-FeOOH)와 망간산화물(manganese oxide, ${\lambda}-MnO_2$)을 이용하여 금속환원 실험을 실시하였다. 미생물 활동에 의해 형성된 환원물의 광물학적 특성은 SEM, EDX, XRD 분석을 통해 확인되었다. 연구 결과 KURT 지하수에서 금속을 환원하는 혐기성 미생물로는 Fusibacter, Desulfuromonas, Actinobacteria, Pseudomonas sp. 등이 확인되었고, 이 미생물들은 체외에서 철과 망간을 환원하여 이들 광물의 상전이를 확인하였다. 철(Fe)은 $Fe^{3+}$을 포함한 아카가나이트(${\beta}$-FeOOH)에서 $Fe^{2+}/Fe^{3+}$를 포함한 자철석($Fe_3O_4$)으로 환원되었고, 망간(Mn)은 $Mn^{4+}$를 포함한 망간산화물(${\lambda}-MnO_2$)에서 $Mn^{2+}$을 포함한 능망간석($MnCO_3$)으로 환원되었다. 이러한 지하 140 m의 KURT 지하수에서 서식하는 미생물들에 의해 철과 망간이 환원됨은 다른 중금속과 핵종원소의 환원 가능한 환경이 조성되었을 뿐 만 아니라, 미생물에 의하여 환원된 철의 재산화에 의해서도 주변 핵종원소가 환원될 수 있음을 의미한다. 따라서 이러한 직 간접적인 산화-환원 반응에 의해 KURT 지하수 내에서는 금속환원미생물들이 유해금속물질을 침전시켜 이동성을 줄일 수 있을 뿐만 아니라 고준위 폐기물에서 유해물질의 유출시 핵물질의 확산을 막는데 중요한 역할을 할 수 있을 것으로 사료된다. The purposes of this research were to investigate the enrichment of metal-reducing bacteria from KURT groundwater and the identification of the microbial diversity by 16S rRNA as well as to examine microbial Fe(III)/Mn(IV) reduction and to analyze morphological features of interactions between microbes and precipitates and their mineralogical composition. To cultivate metal-reducing bacteria from groundwater sampled at the KURT in S. Korea, different electron donors such as glucose, acetate, lactate, formate, pyruvate and Fe(III)-citrate as an electron accepter were added into growth media. The enriched culture was identified by 16S rRNA gene sequence analysis for the diversity of microbial species. The effect of electron donors (i.e., glucose, acetate, lactate, formate, pyruvate) and electron acceptors (i.e., akaganeite, manganese oxide) on microbial iron/manganese reduction and biomineralization were examined using the 1st enriched culture, respectively. SEM, EDX, and XRD analyses were used to determine morphological features, chemical composition of microbes and mineralogical characteristics of the iron and manganese minerals. Based on 16S rRNA gene analysis, the four species, Fusibacter, Desulfuromonas, Actinobacteria, Pseudomonas sp., from KURT groundwater were identified as anaerobic metal reducers and these microbes precipitated metals outside of cells in common. XRD and EDX analyses showed that Fe(III)-containing mineral, akaganeite (${\beta}$-FeOOH), reduced into Fe(II)/Fe(III)-containing magnetite ($Fe_3O_4$) and Mn(IV)-containing manganese oxide (${\lambda}-MnO_2$) into Mn(II)-containing rhodochrosite ($MnCO_3$) by the microbes. These results implicate that microbial metabolism and respiratory activities under anaerobic condition result in reduction and biomineralization of iron and manganese minerals. Therefore, the microbes cultivated from groundwater in KURT might play a major role to reduce various metals from highly toxic, mobile to less toxic, immobile.

대전, 충청 지역에서 판매되는 즉석식품의 트랜스지방 및 조지방 함량 모니터링

김유미,허옥순,이기택,Kim, Yu-Mi,Heo, Oak-Sun,Lee, Ki-Taek 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.8

This study was designed to determine the trans fatty acid (tF A) contents of 41 take-out food items in Daejeon and Chungcheong Province. Total fatty acid composition and tFA contents of samples were determined by gas chromatography (GC) after fat extraction (Folch method). Maximum fat content among the observed samples was obtained from theater popcorn (13.74${/cdot}$38.09%) while minimum content was observed from the glutinous rice doughnut. 31${/cdot}$5.44%). The contents of tFA (g/100 g food) in toasts, glutinous rice doughnut and ggwabaegi (twisted doughnut sold in streets) ranged from 0.02 to 0.56 g while those in fried sweet potatostick (from highway resting place), fried squid and theater popcorn ranged from 0.05 to 3.08 g/100 g. As a result, most samples showed the tFA content as less than 1 g (g/100 g food) except one sample from theater popcorn which contained 3.08 g (g/100 g food) as tFA content. 대전 및 충청 지역에서 판매되고 있는 즉석식품의 조지방 및 트랜스지방산의 함량을 조사하고자 찹쌀도넛과 꽈배기 각각 6종, 고구마 스틱 2종, 토스트 9종, 그리고 멀티플렉스 극장 팝콘 11종 등 총 41종을 수거하여 분석하였다. 조지방 함량은 멀티플렉스 극장 팝콘 13.74${\sim}$38.09%로 가장 높았으며, 고구마 스틱 20.82${\sim}$20.88%, 오징어 튀긴 9.37${\sim}$25.53%등의 순이었다. 수거한 시료는 유탕처리 식품이었으며, 멀티플렉스 팝콘은 전자렌지용 팝콘과 달리 트랜스지방산 함량이 매우 적게 분석된 것으로 보아 식물성 부분 경화 유지를 상요하지 않은 것으로 보인다. 트랜스지방산 함량은 대부분 1 g(g/100 g food) 미만으로 낮았으나, 멀티플렉스 극장 팝콘 중 한 시료만이 3.08 g(g/100 g food)으로 높게 나타났다.