The effect of combined Rehmannia glutinosa Libosch and Eleutherococcus senticosus Max(OPB) extracts on bone mineral density in ovariectomized rats.

Kim,Jung-Keun 대한구강생물학회 2007 International Journal of Oral Biology Vol.32 No.4

This study was conducted to investigate the preventingeffects of OPB (Rehmannia glutinosa Libosch and Eleuth-erococcus senticosus Max extracts) and combined OPB/Calcium therapy on bone loss in ovariectomized rats. SixtySprague Dawley rats of 12-week-old were divided into eightgroups: OVX (ovariectomized), OPBL (OPB 50mg/kg),OPBM (OPB100mg/kg), OPBH (OPB 200mg/kg), OPBL/CAL(OPBL+CAL), OPBM/CAL (OPBM+CAL), OPBH/CAL (OPBH+CAL) and CAL (Calcium citrate 88.33mg/kg+1α, 25-dihydroxy-vitamin D3 33.33IU/kg). Bone mineraldensity (BMD), bone mineral content (BMC), bone strengthindices and cortical thickness were analyzed by peripheralquantitative computerized tomography (pQCT). pQCTscanning showed that OVX induced a significant decreasein trabecular bone mineral density and bone mineralcontent in the proximal tibia (-36.4±2.4%, -21.8±12.7%).These decreases were significantly prevented by theadministration of OPBM and OPBM/CAL. Cortical BMDand BMC of tibia were slightly enhanced by OPB and OPB/CAL. However there was no significant difference betweenOVX and OPB, OPB/CAL treated group. Bone strengthindices and cortical thickness were not significantly different.Our results suggest that OPB and combined OPB/Calciumtherapy are effective in preventing the development of boneloss induced by ovariectomy in rats.

뼈흡수유도호르몬이 ROS17/2.8세포로부터 Nitric Oxide 형성에 미치는 영향

고선일,김민성,한원정,김세원,김정근 대한구강악안면방사선학회 2005 Imaging Science in Dentistry Vol.35 No.3

Purpose : We performed the present study to investigate whether osteotropic hormomes play roles on the nitric oxide (NO) production in culture of ROS17/2.8 osteoblastic cells. Materials and Methods : The osteoblastic cell line ROS17/2.8 cells were cultured in F12 medium supplemented with 5% fetal bovine serum (FBS) at 37?C in a humidified atmosphere of 5% CO₂ in air. ROS17/2.8 cells were plated in 96-well plates at a density of 2-3×10³ cells/well and grown to confluence. Then the cells were pretreated with osteotropic hormones (parathyroid hormone (PTH) 20-500 ng/mL, 1,25 dihydroxycholecalciferol (1,25[OH]₂D₃) 1-100 nM; prostaglandin E₂ (PGE₂) 20-500 ng/mL) in the medium supplemented with 0.4% FBS for 72 hours and the cells were treated with cytokines (TNFα and IFNγ) in phenol red-free F12 medium for an additional 48 hours. NO synthesis was assessed by measuring the nitrite anion concentration, the reaction product of NO, in the cell culture medium using Griess reagent. Results : PTH and 1, 25[OH]₂D₃ pretreatment induced a significant increase in NO production in the presence of TNFα and IFNγ. PGE₂ slightly induced NO production compared to the control group. But, PGE2 pretreatment did not affect in NO production in the presence of TNFα and IFNγ. Conclusions : These results suggest that the actions of osteotropic hormones in bone metabolism may be partiallymediated by NO in the presence of cytokines.

CT-26 선암을 접종한 마우스에서 Iodine-131-Iodomisonidazole 의 생체분포 및 종양저산소증의 영상화

Yang, David J,Kim, E Edmund,김혜원,김창근,윤권하,김현정,정선관,노병석,Lee, Hyun-Chul 대한핵의학회 1999 핵의학 분자영상 Vol.33 No.3

urpose: Misonidazole is a radiosensitizer that binds in hypoxic cells. The purpose of this study was to find out the feasibility of I-131-Iodomisonidazole (IMISO) for imaging of tumor hypoxia. Materials and Methods: Tosyl precursor was dissolved in acetonitrile and I-131-NaI was added to synthesize IMISO. Balb/c mice inoculated with CT-26 adenocarcinoma were injected with IMISO. Mice were sacrificed at 1,2,4,24 hr and % of injected dose per gram of tissue (%ID/g) was determined. For scintigraphy and MRI, mouse bearing CT-26 adenocarcinoma was administered with IMISO and imaging was performed 4 hr after. Then, mouse body was fixed and microtomized slice was placed on radiographic film for autoradiography. Results: %ID/g of tumor was 1.64 (1h), 0.98 (2h), 0.85 (4h) and 0.20 (24h), respectively. At 24h, %ID/g of tumor was higher than that of all other tissues except thyroid. Tumor to muscle ratio increased with time and tumor to blood ratio also increased with time and reached 1.53 at 24 hr. On autoradiogram, tumor was well visualized as an increased activity in central hypoxic area of the tumor which corresponds to the area of high signal intensity on T2-weighted MR image. On scintigraphy, tumor uptake was visualized. Conclusion: This results suggest that IMISO may have a potential for tumor hypoxia imaging in mouse model. However, further study is needed to improve it's localization in tumor tissue and to achieve acceptable images of tumor hypoxia.

연령과 속도 조건이 축구 패스의 타이밍 정확성에 미치는 영향

김원대,김선진 한국스포츠심리학회 2003 한국스포츠심리학회지 Vol.14 No.3

본 연구는 다양한 속도 조건에서 연령이 증가함에 따라 나타나는 타이밍의 시간 정확성과 타이밍 일관성의 발달적 변화 경향을 구명하는데 그 목적이 있다. 축구의 패스 과제와 버튼 누르기 과제를 본 연구 과제로 설정하였으며, 연구 대상자는 9세, 11세, 13세, 15세의 4개 연령별로 15명씩 총 60명으로 하였다. 자극의 속도는 시속 1마일, 4마일, 7마일, 10마일 네 가지 속도 조건을 무선적으로 제시하였으며 버튼 과제는 각 속도별 5회씩 총 20회, 패스과제는 각 속도별 9회씩 총 36회를 실시하였다. 예측 반응 측정기(Bassin Anticipation Timer)를 이용해 자체 제작한 타이밍 정확성 검사 도구를 통하여 타이밍 정확성의 시간 오차를 산출하였고 산출된 오차를 절대오차, 항상오차, 가변오차로 분류하여 연령과 속도 조건별로 이원 변량 분석(Two-way ANOVA)을 실시한 결과, 유소년의 패스 과제 수행 시 타이밍의 시간 정확성은 연령이 증가할수록 향상을 나타냈으며 시간 타이밍이 가장 정확하게 나타나는 속도 조건은 시속 4마일이었다. 버튼 과제 수행 시 타이밍의 시간 정확성은 패스 과제와 마찬가지로 연령이 증가할수록 유의하게 향상되었다. 패스 과제 수행 시 타이밍의 일관성은 11세 이후 연령이 증가할수록 향상되었으며 이때의 타이밍 일관성은 시속 10마일에서 가장 높게 나타났다. 버튼 과제 수행 시 타이밍 일관성은 빠른 속도 조건(시속 7마일과 10마일)일 때만 연령이 증가할수록 향상되는 모습을 보였다. The purpose of this study was to examine the effects of age and speed of stimulus on coincidence-anticipation timing. There were four groups consisting of 15 boys in each group. The ages of each group were 9, 11, 13, and 15. The tasks were a push of a button and a pass of a soccer ball. The speed of stimulus were four conditions: 1, 4, 7, and 10mph(mile/hour) and randomly given to the subjects. All participants executed 20 trials(5 trials per each speed) of a button task and 36 trials(9 trials per each speed) of a pass task. The timing error of coincidence- anticipation task was recorded by using Bassin Anticipation Timer, and then AE(absolute error), CE(constant error), and VE(variable crror) were computed. The data were analyzed by descriptive statistics and two-way analysis of variance (ANOVA) of AE, CE, and VE. The result of this study is following: First, in a pass task, timing accuracy improved significantly as the age increased and showed the highest value at 4mph of speed. Second, in a button task, timing accuracy improved significantly as the age increased and showed the highest value at 8 and 9mph of speed. Third, in a pass task, thiming consistency improved only after 11 years of age as the age increased and showed only at 7mph and 10mph as the age increased and showed the highest value from 7mph to 10mph of speed.

간 허혈성괴상의 CT소견에 대한 실험적 연구 : 간실질 및 피막의 경시적 변화와 병리조직의 비교 Changes of CT Findings of Liver Parenchyama and Capsule with Time Lapse Correlated with Histopathology

김창근,김강득,최시성,정선관,노병석,원종진 圓光大學校 醫科學硏究所 1994 圓光醫科學 Vol.10 No.1-2

In order to evaluate CT findings with time and to confirm the capsular structure correlating with its histopathologic findings, we experimentally induced ischemic necrosis of liver in 20 rabbits. Incision of the abdomen was performed under anesthesia. Ligation of hepatic artery, portal vein and hepatic duct of right inferior posterior lobe of the liver after injection of macroaggregated albumin into right inferior posterior portal vein in order to prevent from developing collateral circulation as possible. Precontrast and postcontrast enhanced CT images were obtained at 1. 2. 4 days and 1, 2. 4. 6 weeks after induction of ischemic necrosis. Necrotic area in the liver showed homogeneous low density compared to normal liver, and no contrast enhancement after contrast material administration, and marked volume loss with time. CT attenuation value of necrotic area at 1 day(36.7±6.4 M±SD, Hounsfield unit) approached to two third of that of normal liver(56.3±7.3) and showed no significant change until two weeks but increased significantly after four weeks. On contrast enhanced CT images, capsular structure was obsereved as a high density rim along the liver surface of the necortic area in 8 of 15 cases. From the first day on, homogenous ischemic or coagulation necrosis was observed but rarely findings of edema, hemorrhage and cystic change were observed on histopathologic specimen. In all of the histologic samples taken from 10 cases during th 2nd to 6th week, fibrous thickening with rich vascularity was observed along the surface of the necrotic area. In conclusion, ischemic necrosis of the liver showed homogeneous low density and no contrast enhancement. CT attenuation value of necrotic area increased significantly with time associated with markedly decrease in volume. Capsular structure attributed to the hypertrophy of the liver capsule and rich vascularity within it.

초경 시작 시기가 여성 정신분열병 환자의 병의 경과에 미치는 영향

김진훈,윤선욱,배명지,임선진,정은기,장동원 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.5

Objectives : The protective effects of estrogen against the development of schizophrenia have been investigated in various perspectives, Previous studies showed patients with earlier age of menarche had later onset of schizophrenia and less frequent admissions. This study was performed to evaluate the effect of the age of menarche on subsequent courses including hospitalizations, suicidal attempts, Physical harm to others, and psychotic symptoms in women with schizophrenia. Methods : Self-report menarche age, onset of psychotic symptoms, and clinical status were collected in a sample of 108 Premenopausal women with the diagnosis of schizophrenia. Results : There was significant correlation between the age of menarche and the frequency of admissions (r=.321, p=.006). Also, Patients with history of physical harms to others showed later age of menarche than those without (t=2.025, df=106, p=.045). Conclusion : In women with schizophrenia, the age of onset of menarche affects subsequent courses indicated by frequency of admissions and violent behaviors. It is suggested that identifying the age of menarche might be helpful to predict the courses in women with schizophrenia. Further investigations regarding the relationship between estrogen and the courses of Schizophrenia in women are needed.

열파이프의 위크현상에 따른 전열특성

김성원,이진호,강신형 연세대학교 산업기술연구소 1990 논문집 Vol.22 No.2

Experimental investigation is made to study the dependence of performance characteristics of heat pipe on the types of wick shapes. Types of wick shapes adoped are open groove wick, screen wick, closed groove wick and no wick.(thermo-syphone). The dependence of heat pipe performance on the wick shape is turned out to be in the following order ; open groove wick, closed groove wick, screen wick and no wick. This shows that the heat transfer efficiency of heat pipe depends more upon the returning capacity of liquid from condenser to evaporator, implying that the wick which has low capillary pressure but good permeability is better than those which has higher capillary pressure.

Beta-차단제와 Hydrochlorothiazide 倂用에 依한 본태성 고혈압 환자의 血淸脂質에 미치는 效果

金善政,李宗經,劉元相 인제대학교 1984 仁濟醫學 Vol.5 No.4

Serial changes of serum lipids were observed in 54 patients with essential hypertension, administered several beta-blockers (e.g. acebatolol 400mg, atenolol 50mg, pindolol 10mg, propranolol 160mg, respectiviely) combined with hydrochlorothiazide 25mg for up to 12 weeks. The results were as follows: 1.The blood pressure was normalized at 12 weeks except propranolol group. 2.Seroum total cholesterol was decreased to 11% in both pindolol group and propranolol group, and not remarkable in other groups. 3.Triglyceride was decreased in propranolol group to 50% at 6week and 12 weeks, and not remarkable in other groups. 4.HDL-Cholesterol was decreased from 5% to 20%, most remarkable in propranolo group and decreased in pindolol group to 5% only. 5.Cholesterol ratio was increased only in pindolol group to 4.5% and decreased from 5% to 10% in other groups. In summary, these mild changes of serum lipid profiles alarm the physicians to check serum lipids before and during medication, especially in youger hypertensive and hyperlipidemic patient.

알루미늄 합금을 이용한 초소형 사각컵 성형품의 특성

김원일,이승준,이정환,이영선 慶南大學校 附設 工業技術硏究所 1999 硏究論文集 Vol.17 No.-

벌지 성형기술이 상업적인 초소형 성형공정에 널리 사용되고 있지만, 일축 인장시험은 재료의 초소성을 평가하는 방법으로 아직도 가장 일반적으로 사용되고 있다, 그러나 1축 인장시험으로부터 얻은 결과들은 다축 응력상태의 실제 성형품의 특징을 해석하는데는 적용할 수가 없다. 본 논문은, 다축 응력상태로 초소성 성형에 의해 만들어진 사각컵 성형품으로부터 얻은 인장시험시편을 사용하여 변형률과 공동의 량에 따라 인장강도와 연신율을 조사하였다. 이 실험의 결과로부터 Al-6%Cu-0.4%Zr 알루미늄 합금의 인장강도와 연신율은 변형률과 공동에 따라 감소하였다. Although the bulge forming technique is currently employed in commercial superplastic forming processes, the uniaxial tensile test is still the most commonly used method for the evaluation of the superplasticity of materials due to its simplicity in testing. However, the results obtained from the uniaxial tensile test can not be applied in analyzing the characteristics of the real parts formed in multi-axial stress state. In this paper, using the tensile test specimen obtained from the squats·cup manufactured by superplastic forming in multi-axial stress state, tensile strength and elongation have been investigated according to the strain and cavity volume fraction. From the result of experiment. tensile strength and elongation are decreased according to the strain and cavity in Al-6% Cu-O.4%Zr alloy.

피부 스포로트리쿰증에서 Itraconazole 경구 투여의 효과에 대한 관찰

원영호,전선도,김성진,이승철 대한의진균학회 1998 대한의진균학회지 Vol.3 No.2

Background: The effectiveness of some therapeutic modalities for cutaneous sporotrichosis may be changed depending on the individual and geographic characteristics, and the clinical type. Itraconazole has been known to be effective for it, however, there have been no standard and ideal guideline on the oral dosage and duration yet. Objective 8: Methods: In this study, we evaluated the effectiveness of oral itraconazole in patients with cutaneous sporotrichosis in relation to dosage and clinical status. The observation of 17 patients with sporotrichosis were made retrospectively, Oral itraconazole was administered at the dose of 200 mg daily in 15 adults over 18 years old and 100 mg in the other 2 children. Results: The duration for healing was within 30 days in 4 cases, within 60 days in 4 cases, within 90 days in 2 cases, within 120 days in 3 cases, and within 150 days of therapy in 2 cases. The average duration in all cases was 12.1 ± 8.6 weeks. Comparison our results to the previous reported one revealed that more shorter treatment time was needed in cases treated with 200 mg daily than that with 100 mg. There was no treatment failure by drug intolerance or resistance. The duration for healing was significantly shorter in the patient groups with prevalence duration more than 5 months, however there was not any significant difference in sex of patients, and the clinical types. The cases with the lesion on the face and cases with fixed type tended to be healed more rapidly than the others, Conclusion: Oral itraconazole may be a safe and effective antifungal agent for the treatment of cutaneous sporotrichosis in a daily dose of 200 mg for 3 months on the average. However, duration for healing could be influenced by the clinical status.