Plant regeneration from callus of Iris odaesanensis Y. N. Lee native to Korea via organogenesis

Bae, Kee-Hwa,Yoo, Kyoung-Hwa,Lee, Mi-Hyun,Jeong, Jae-Hun,Choi, Yong-Eui,Yoon, Eui-Soo The Korean Society of Plant Biotechnology 2013 식물생명공학회지 Vol.40 No.3

Iris odaesanensis Y. N. Lee. is an important endangered and native plant belonging to the family Iridaceae in Korea. This study describes a method for rapid micropropagation of this species via from leaf, rhizome and root explants derived calli. Leaf, rhizome and root explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) for callus induction. Rhizome explants yielded calli at a frequency of 72% when cultured at 1.0 mg/l 2,4-D. Calli were maintained at 1.0 mg/l 2,4-D. These calli were transferred to MS medium supplemented with 0, 0.5, 1.0, and 2.0 mg/l 2,4-D in combination with 0, 0.5, 1.0, and 3.0 mg/l BA for adventitious shoot induction. The highest number of adventitious shoot (228.9 per petri-dish) were formed at 1.0 mg/l 2,4-D and 1.0 mg/l BA. WPM medium was the best to convert calli into plantlets, where up to 98.2% of calli were regenerated into plantlets. This in vitro propagation protocol should be useful for conservation of this endangered plant.

糞便檢査에 있어서 세로판 厚層塗抹法과 簡易食鹽水浮游法의 比較檢討

李駿商,張基浩,朱炅煥,金洙鎭,林漢鍾 고려대학교 의과대학 1979 고려대 의대 잡지 Vol.16 No.1

Experiments had been made to evaluate more efficient method in stool examination for various helminth ova. Authors examined 2,016 nationwidely collected stool specimens by the two methods, cellophane thick smear method and brine floatation method. The results were as foliows; 1. Of 2,019 specimens examined by cellophane thick smear method, the positive rates of following helminth ova were; Ascaris lumbricoides (fertilized) 27.3% Ascaris lumbricoides (unfertilized) 13.6%, Trichuris trichiura 39.0%, Hookworm 0.1%, Enterobius vermicularis 0.05% Hymenolepis nana 0.04%, Taenla spp. 1.3% Clonorchis sinensis 1.1% and Metagonimus yokogawai 5.7%. And, A. lumbricoides (fertilized) 27.0%, A. lumbricoides (unfertilized) 3.8%, T. trichiura 38.1%, Hookworm 2.0%, Trichostrongylus orlentalis 0.6%, E. vermicularis 0.2%, H. nana 0.8%, and M. yokogawai 0.05% by brine floatation method. 2. The causes of inferior positive rates in some helminth ova by cellophane thick smear method were shortly discussed and suggest some opinions in improvement of detection ability. 3. The positive rates of following helminth ova were superior in cellophane thick smear method to those of brine floatation method: A. lumbricoides(fertilized), Taenia spp., C. sinensis and M. yokogawai. And, the positive rates of following helminth ova were superior in brine floatation method: Hookworm T. orientalis, H. nana and E. vermicularis. 4. Cellophane thick smear method has been known to be one of the most effective methods in mass stool examination. But, this method is not so effective in detection of certain species of helminth ova. Therefore, employment of brine floatation method added to cellophane thick smear method is desirable for the better result of mass stool examination.

주정박 사일리지 제조시 인삼박의 혼합이 사일리지의 품질에 미치는 영향

이수기,이인덕,김용국 충남대학교 농업과학연구소 2001 농업과학연구 Vol.28 No.1

본 연구에서는 타피오카 주정박에 인삼박을 혼합하여 사일리지를 제조하였을 때, 사일리지의 품질(실험 Ⅰ)과 건물 섭취량 및 소화율 (시험 Ⅱ)에 미치는 영향을 조사하였다. 사일리지 조제시 수분함량을 낮추기 위하여 소맥피를 10% 수준으로 첨가하였으며, 인삼박의 혼합비율은 원물기준으로 0, 15 및 30% 수준 (3 처리)으로 하였다. 처리당 5반복으로 20㎏ 씩 비닐백에 넣고 공기를 배제하여 포장한 후 20∼25℃에서 30일간 숙성시켜 공시하였다. 시험 Ⅱ의 소화시험에서는 10개월령의 재래산양 수컷12두 (평균체중 14㎏)를 공시하여서 처리당 4반복이 되게 하였다. 7일 동안의 적응기간을 거친 후, 5일간 소화율을 조사하였던 바, 얻어 진 결과를 요약하면 다음과 같다. 1. 인삼박의 혼합 비율이 증가함에 따라 조단백질, NDF 및 ADF의 양이 증가하는 경향을 나타내었으며, 특히 인삼박 30% 첨가구는 인삼박 무첨가구와 인삼박 15% 첨가구에 비하여 유의하게 높은 결과를 나타내었다 (P<0.05). 그리고 조지방 함량과 hemicellulose 함량은 처리구간에 유의한 차이가 인정되지 않았다. 2. pH는 인삼박을 혼합하므로서 유의하게 감소하였으며, 인삼박 처리구간에는 유의한 차이가 인정되지 않았다. 젖산 함량은 인삼박 혼합비율이 증가함에 따라 유의하게 많아졌으며 (P<0.05), 총산도 인산박 첨가구가 무첨가구에 비하여 유의하게 많은 결과를 나타냈다. 초산은 인삼박 30% 첨가구가 유의하게 적었다. 한편 낙산은 인삼박 첨가구가 무첨가구에 비하여 유의하게 적었다(P<0.05) 3. 사일리지의 섭취량은 인삼박 첨가구가 무첨가구 보다 유의하게 많았다. 건물 소화율은 인삼박을 많이 혼합할수록 증가하였다. 위의 결과로 볼 때 주정박에 인삼박을 혼합하여 사일리지를 조제하는 것은 주정박 안으로 조제할 때 보다 더 좋은 품질의 사일리지를 만들 수 있으며, 기타 유사 재료에 대해서도 광범위한 적용이 가능할 것으로 사료된다. Two experiments were conducted to investigate the effects of the addition of ginseng meal to distillers feed on the silage quality, and feed intake and matter (DM) digestibility in goats In Experiment I , three levels of ginseng meal (0, 15, and 30%) were added to the distillers feed to make three types (treatment) of distillers feed silages (DFS). There were five replicates per treatment. Samples of 20 kg each were put into vinyl bag and vacuumed by an air compressor Vinyl bags mere kept at 20-25℃ for 30 days before being analyzed In Experiment Ⅱ. 12 male Korean native goats were employed to investigate the DM intake and digestibility of silages from Experiment I There n-ere 4 replicates per treatment. Daily feed intakes and fecal excretion were measured In Experiment I. the silage pH decreased and the lactic acid increased significantly by the addition of ginseng meal (P<005) In Experiment Ⅱ, the feed intake and DM digestibility increased significantly by the addition of ginseng meal It appears that the quality of distillers feed silages could be improved by the addition of ginseng meal at the level of 15 to 30%.

腎穿細胞腫(Wilms' Tumor)8例의 病理學的 檢索

李聖洙,裵奇煥 醫藥係社 1958 綜合 醫學 Vol.3 No.6

北韓의 行政體系의 變化에 관한 硏究

李聖九,張基秀 弘益大學校 1987 弘大論叢 Vol.19 No.1

The major traits of the North Korean administrative organization can be listed as follows. To begin with, its power structure consists of dualistic system-the Party and the governmental organ. The organic interrelatedness between the Party and the governmental organ has always been strongly stressed. And the organic interrelatedness between them is considered, can most successfully be realized only though the merging of both heads of the party and the government into one. The control of administration by the Party is most complete. And in order to secure the control, the governing power of the administrative organs stems directly from the Party. The administrative system of North Korea has undergone reorganizations as many as eight times since its establishment. The reorganizations of the governmental organ, however, have all been carried out in order to suit and satisfy the aims solely of Kim Il-Sung. Another aspect to be noted is that the first revolutionary generation is lagely replaced by the younger generation comprising bureaucrats of either the Party or the government. As a result, the general trend is toward 'bureaucratization' or the progressive transformation of politics into administration. In terms of organizational change in the North Korea's governmental structure, the lack of efficiency, specialization, effectivity in its structures is noticeable. To make up for these weakness, the decentralization and autonomy of its structure is urgently called for-at least in the substructures beneath the State Cabinet. What obstructs the way, however, (the State Council) is the ever present necessity of strong control over the governmental bodies in order to secure its stability, for the long-drawn-out Kim Il-Sung regime has of necessity raised such harassing problems as the threatening signs of seditions and as the undermining counter-effects. This is the serious dilemma in which the North Korean government finds itself.

혈전 발생의 위험 인자가 없는 환자에서 급성 신우신염에 의해 유발된 신정맥 혈전증 및 폐색전증

이광수,김아름,이승연,정주홍,김미정,이진수,정문현,홍기천,조순구 대한감염학회 2009 감염과 화학요법 Vol.41 No.5

Renal vein thrombosis (RVT) is not an uncommon condition amongst patients with nephrotic syndrome or malignancy. Septic pulmonary embolism (SPE) is associated with risk factors such as intravenous drug use, pelvic thrombophlebitis, and suppurative processes in the head and neck. However, acute pyelonephritis is a rare cause of RVT and SPE. Case reports on RVT and SPE due to acute pyelonephritis are rare. In most of the earlier cases, patients had underlying conditions such as diabetes mellitus, renal carcinoma, calyceal stones, and hyperhomocysteinemia. We report a case of acute pyelonephritis complicated by RVT and SPE that occurred in a patient without any predisposing risk factors for thromboembolism. RVT and SPE were diagnosed using computed tomography and ventilation/perfusion scan. The patient recovered with antibiotics and anticoagulation therapy without any surgical interventions.

Magnetron sputtering법으로 제조된 Al-1%Cu/Tungsten Nitride 다층 박막

이기선,서수정,김장현,김남철 공주대학교 자원재활용신소재지역협력센터 2000 2차년도 센터 사업 성과집 Vol.2000 No.1

표면 탄성파 디바이스의 전극재료로 사용되는 Al-1%Cu(4000Å)/tungsten nitride 박막을 magnetron sputtering법으로 제조하고 전기저항을 평가한 결과 비정질상의 tungsten nitride 박막을 제조할 수 있었고, 비정질 형성을 위해서 질소비 (R = N2/(Ar+N2))가 10∼40% 정도 필요하였다. Tungsten nitride 박막의 잔류응력은 비정질이 형성되면서 급격히 감소되었다. 이러한 비정질 박막위에 Al-1%Cu합금막이 형성되었다. 다층막은 453K에서 4시간 동안 열처리함으로써 3.6μΩ-cm의 저항를 나타냈는데, 이는 박막내 결정립 성장과 결정 결함의 감소에 기인하였다. As a power durable-electrode in SAW filter. Al-1%Cu/tungsten nitride multi-layer thin film was fabricated by magnetron sputtering process. Tungsten nitride films had the amorphours phase at the nitrogen ratio, R, ranging from 10~40%. The amorphization could be controlled by nitrogen ratio. R = N2/(N2+Ar) as a sputtering process parameter. Residual stress in tungsten nitride abruptly decreased with the formation of amorphous phase. Al-1%Cu thin film was deposited on the amorphous tungsten nitride. After the multi-layed thin film was annealed for 4 hours at 453K, the resistivity decreased as 3.6 μΩ-cm, which was due to grain growth and reduced crystal defects.

질화 분위기에서 결정화 된 Fe_(73.5)Si_(13.5)B_(9)Cu₁Nb₃ 연자성 합금의 자기적 성질

이기선,박인수,이기안,김문철,장석훈 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.11

Amorphous Fe_(73.5)Si_(13.5)B_(9)Cu₁Nb₃ alloy was crystallized in nitriding atmosphere of mixture gas of NH₃ and hydrogen. The crystallization at 823K showed the high electrical resistivity ranging from 494 to 538 mW-cm, which was higher level compared with nano-crystalline Fe_(73.5)Si_(13.5)B_(9)Cu₁Nb₃ alloy with a resistivity of about 110 mW-cm. Through the nitridation treatments, the amorphous alloy was transformed into nano-sized crystalline Fe3Si including iron nitrides such as γ' -Fe₄N phases. EPMA-concentration depth profiles confirmed an existence of nitrogen-rich region on the surface of the alloy ribbon, leading to the preferential formation of g -Fe4N phase. The formation of γ' -Fe₄N phase leaded to an increase of the resistivity and provided stable permeability and a low core loss at high frequency.

대전광역시 양축농가의 축분뇨 관리 실태 및 VOCs 농도 조사

이봉덕,이수기,오홍록,허정민,정기철,김성복 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.1

This study was carried out to investigate the style of livestock house, concentration of malodorous substances of livestock feces and livestock houses in Daejeon area. Among the livestock houses investigated, as most of cow pens(94.5%) have sawdust or chaff on the bottom, there was no leakage of feces out of pen. Most pig pens adopted slury style, but some of them currently use buffering material on the bottom. It is thought that there will be no possible contamination leakage. When it comes to hen house, all the broiler house use litters on the bottom and all the layer house use scrapper. It is also thought that there will be no possible contamination leakage. 3 out of 12 deer pens used buffering material on the bottom, 10 places were maintained in a traditional method, and 7 places left possibility of contamination leakage considering whether the roof was installed or not. The contents of ammonia, amine and volatile fatty acid in fresh feces were lower compared to rotten feces, but the concentration of sulfur-containing matter - hydrogen sulfide, methylmercapthan and ethylmercapthan were higher compared to rotten feces. In the case of malodorous ingredient in livestock houses, only small amount of ammonia and hydrogen sulfide were detected in pig pen and hen house, and other ingredients were not detectable. And those who are engaged in animal husbandry reacted negatively to the use of feed additives for decreasing malodor. In conclusion, it is not worrisome that contamination can be leaked out of animal raising facilities. But if we take into consideration that the point of investigation time is wintry season, there should be more considerate attitude. And feed additives for decreasing malodor need establishing criteria in the manufacturing process.

생쥐 대장 평활근 세포에서 내향 정류성 칼륨 전류의 특성 연구

이은주,김명,정명섭,조향훈,김기훈,하현철,김준수,최석,전제열 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Objectives: K^(+) channels play an important role in regulating cellular excitability. The aim of this study was to know whether or not inward rectifier K^(+) channel exists in colonic smooth muscle cells. Methods: Mouse colonic smooth muscle cells were isolated using collagenase, and then we recorded their membrane currents using a whole-cell patch clamp technique. Results: With 90 mM K^(+) in bath, hyperpolarization-induced inward currents from -120 mV to 20 mV with 400 ms duration at a holding potential of -10 mV showed rapid activation, inactivation and inward rectification. The inactivation showed single exponential time course. Reduction of external K^(+) to 60 mM decreased the amplitudes of the currents in whole test voltage range and shifted the reversal potential to more negative potential. The inactivation process and peak currents of hyperpolarization-induced inward currents were not affected by removing external Na^(+). External Ba^(+) blocked hyperpolarization-induced inward currents by dose-dependent manner and pure Ba^(+)-sensitive currents showed strong inward rectification. Cs^(+) also suppressed hyper- polarization-induced inward currents. Ba^(+) and Cs^(+)-induced inhibitiOn of hyperpolarization-induced inward currents was voltage-dependent, and the extent of inhibition increasing with membrane hyperpolarization. Conclusions: These results suggest that inward rectifier KU channels may exist in proximal colonic smooth muscle and may play an important role in regulating membrane potential.