프로야구 손상의 임상적 분석

김철준,김명화,김미정,신명진,윤준오,김상규 대한스포츠의학회 1995 대한스포츠의학회지 Vol.13 No.1

Baseball is rapidly getting popularity in Korea as it has been in U.S.A and Japan. During the last decade, threre were several studies about baseball injuries which oriented mainly to the overuse injuries of growing baseball players. But there was no study the professional baseball players systematically. This study was surposed to see the general characteristics of baseball injuries and to provide the prevention and rehabilitation programs if recommended. The number of 83 injuries of 60 baseball players was analysed according to the position, injury site, diagnosis, injury mechanism and duration of injury. The mean age and height of 60 subjects were 24.6 years old and 180.65㎝, respectively. The baseball position of 82 injured cases were composed of 27 pitchers, 30 infielder, 19 outfielder and 6 catchers. The characteristics of injury were as follows: 1.The number of chronic injury was 56 cases(68.3%) and the percentage of chronic injury of pitchers was up to 81.5% compared 73.3% of infielder and 47.4% of outfielder. 2. The injury rate of both lower and upper extremitis were equal and the rate of lower back problems was the second, which should be concerned. 3. The number of cases injured by direct collison is as low as 15(18.3%). The most common secondary cause of injury was aggressive throwing itself and 26 out of 49 cases injured not by direct collision. 4. More concern should be payed about the low back problems and about the catcher and the field players as much as pitchers from now one.

Brucella abortus 국내 분리주의 36kDa 외막단백을 암호하는 omp2b 유전자의 염기 분석

허지연,김지영,송근호,김명철,박창식,김태용,김정화,전무형 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

B. abortus 국내 분리주의 분자유전학적 성상을 규명하고 분자역학적 연구에 필요한 기초자료를 얻기 위해 36kDa OMP를 암호하는 omp2b 유전자를 PCR법으로 확인하고,그리고 omp2b 유전자의 핵염기 서열과 아미노산 서열을 결정하고 이 결과를 Brucella 표준균주 및 여러 동물에서 분리된 Brucella 분리주와 상호 비교 분석하였던 바 다음과 같은 결과를 얻었다. 1. B. abortus 분리 주 (7주)에 대해 omp2b primers를 이용하여 PCR을 수행한 바 공시한 참조균주와 분 리균주 모두에서 1,251bp의 특이한 DNA 절편이 각각 증폭되었다. 2. omp2b 유전자의 1,089bp에 대한 염기서열과 이에 대한 362개의 아미노산 서열을 결정하였다. 분리 균주의 omp2b 유전자의 염기서열을 참조균주와 비교 분석한 결과 B. abortus 2308 (biovar 1) 및 B abortus B3196 (biovar 5)과는 99.6%∼100% 및 99.5%∼99.9%의 매우 높은 상동성을 나타내었고,B. abortus 45/20 (biovar 1)과는 97.1%~97.4%의 상동성을 나타내었다. 또한 362개의 아미노산 서열에 대해 분석하였던 바,분리균주들은 B.abortustus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.2%∼100%의 매우 높은 상동성을 보였으며,B. abortus 45/20 (biovar 1)과는 96.4%∼ 97.2%의 상동성을 보였다. 3. 본 시험에 공사된 브루셈라균주의 omp2b 유전자 서열과 여러 동물에서 분리된 브루셀라 분리주의 자료를 바탕으로 phylogenetic tree를 작성 한 바,국내 분리주는 B. abortus 2308 (biovar 1),B. abortus B3196 (biovar 5),B. abortus 45/20 (biovar 1),B. suis 1330 (biovar 1),B. melitensis 16M (biovar 1),B. canis NCTC 01854,B. neotomae 5K33과 97.3% 이상의 높은 근연성을 보이며 동일한 cluster에 소속되었으며,omp2b 유전자는 매우 잘 보존됨을 알 수 있었다. omp2b gene of Brucella abortus isolates encoding 36kDa outer membrane protein (OMP) was amplified by polymerase chain reaction (PCR) and the sequences of nucleotide and amino acid were determined and analyzed to obtain the basic data for molecular epidemiological features of brucellosis in Korea. In PCR using omp2b primers for the gene of 36kDa aMP, the specific signals at 1,251bp were demonstrated, The nucleotide sequences of omp2b gene of 1,089bp were sequenced by dye terminator cycle sequencing method, and 362 amino acid sequences were predicted. As compared the nucleotide sequences with those of B. abortus reference strains, the isolates showed the very high homology to the reference strains ranging 97.1%∼100%. The similar results were also obtained in the analysis of the amino acid sequences. In the phylogenetic tree, the isolates clustered with the reference strains and the majority of strains originated from pig, dog, wood rat and cattle.

Brucella abortus 국내 분리주의 36kDa 외막단백을 암호하는 omp2b 유전자의 염기 분석

허지연,김지영,송근호,김명철,박창식,김태용,김정화,전무형 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.1

B. abortus 국내 분리주의 분자유전학적 성상을 규명하고 분자역학적 연구에 필요한 기초자료를 얻기위해 36kDa OMP를 암호하는 omp2b 유전자를 PCR법으로 확인하고, 그리고 omp2b 유전자의 핵염기 서열과 아미노산 서열을 결정하고 이 결과를 Brucella 표준균주 및 여러 동물에서 분리된 Brucella 분리주와 상호 비교 분석하였던 바 다음과 같은 결과를 얻었다 1. B. abortus 분리주 (7주)에 대해 omp2b primers를 이용하여 PCR을 수행 한 바 공시한 참조균주와 분리균주 모두에서 1,251bp의 특이한 DNA 절편이 각각 증폭되었다 2. omp2b 유전자의 1,089bp에 대한 염기서열과 이에 대한 362개의 아미노산 서열을 결정하였다. 분리균주의 omp2b 유전자의 염기서열을 참조균주와 비교 분석한 결과 B. abortus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.6%~l00% 및99.5%~99.9%의 매우 높은 상품성을 나타내었고, B. abortus 45/20 (biovar 1)과는 97.1%~97.4%의 상품성을 나타내었다. 또한 362개의 아미노산 서열에 대해 분석하였던 바, 분리균주들은 B. abortustus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.2%~l00%의 매우 높은 상품성을 보였으며, B. abortus 45/20 (biovar 1)과는 96.4%~97.2%의 상품성을 보였다. 3. 본 시험에 공시된 브루셀라균주의 omp2b 유전자서열과 여러 동물에서 분리된 브루셀라 분리주의자료를 바탕으로 phylogenetic tree를 작성 한 바, 국내 분리주는 B. abortus 2308 (biovar 1), B. abortus B3196 (blovar 5), B. abortus 45/20 (biovar 1), B. suis 1330 (biovar 1), B. melitensis 16M (biovar 1), B. canis NCTC 01854, B. neotomae 5k33과 97.3%이상의 높은 근연성을 보이며 동일한 cluster에 소속되었으며, omp2b 유전자는 매우 잘 보존됨을 알 수 있었다. omp2b gene of Brucella abortus isolates encoding 36kDa outer membrane protein (OW) was amplified by polymerase chain reaction (PCR) and the sequences of nucleotide and amino acid were determined and analyzed to obtain the basic data for molecular epidemiological features of brucellosis in Korea. In PCR using omp2b primers for the gene of 36kDa OMP, the specific signals at 1,251bp were demonstrated, The nucleotide sequences of omp2b gene of 1,089bp were sequenced by dye terminator cycle sequencing method, and 362 amino acid sequences were predicted. As compared the nucleotide sequences with those of B. abortus reference strains, the isolates showed the very high homology to the reference strains ranging 97.1%-100%. The similar results were also obtained in the analysis of the amino acid sequences. In the phylogenetic tree, the isolates clustered with the reference strains and the majority of strains originated from pig, dog, wood rat and cattle.

입원 노인의 복약지도에 대한 교육요구 및 교육경험

김명신,이미화 노인간호학회 2006 노인간호학회지 Vol.8 No.2

Purpose: This study was a predicative survey to provide elderly hospital in-patients for analyzing their educational needs and educational experience about medication teaching. Method: The subjects consisted of 114 elderly hospital in-patients. Data were collected by researcher, who directly interviewed with them through questionnaires from April 26th to May 7th, from July 20th to Aug. 3th in 2006. The questionnaires for data collection were developed through reference review. The collected data was analyzed by using frequency, percentile, mean, variation, t-test, ANOVA on SPSS 12.0 package. Results: There were past medication related characters of elderly hospital in-patients: circulatory-related illness was the most common disease; 76.3% of them took medicine; 65.8% of them didn't know medicine name; 67.5% of them knew effect of medicine. There were present medication related characters of elderly hospital in-patients: present illness with hospitalized was neurogenic and cerebrovascular disease(59.6%); 95.6% of them took medicine; 90.4% of them didn't know medicine name; 59.6% of them knew effect of medicine. Educational needs of medication teaching were 75.6% and its mean were 94.54±15.54. They required discharging from hospital(4.51±0.68), whenever medicine was changed(4.36±0.79), the effect of medicine (4.35±0.82), the time take medicine(4.31±0.72); the most teaching method they required was “explain with words”(4.20±0.84). Educational experience were 30%. They experienced when hospitalized initially(45.6%), whenever medicine was changed(41.2%), the effect of medicine (64.0%); the most teaching method they experienced was “explain with words”(69.3%). Conclusion: The elderly hospital in-patients educational needs were high, but experience were low. Therefore systematic educational programs about elderly hospital in-patients should be made on the base of this study.

참박과 호박 종자의 발아촉진을 위한 저선량 감마선의 효과

김재성,백명화,이영근,이혜연,유준철 한국환경농학회 2002 한국환경농학회지 Vol.21 No.3

참박과 호박 채종종자의 발아율을 증진시킬 수 잇는 종자 처리 방법을 확립하고자 저선량 감마선을 1∼20 Gy 까지 조사하여 발아와 초기생육 및 생리활성을 관찰하였다. 저선량 조사한 참박과 호박종자의 발아율은 대조구보다 증가하는 경향을 보였는데 효과는 종자상태에 따라 다르게 나타났다. 참박 70∼80% 발아율종자의 발아는 4∼20 Gy에서 증가하였는데 특히 4 Gy조사에서 가장 높았고, 80∼90% 발아율종자도 4 Gy 조사에서 효과를 보였다. 파종 4일 후의 발아율은 참박 70∼80%와 80∼90% 발아율 종자의 경우는 4 Gy조사에서 대조구보다 각각 66%와 26% 증가하였고, 호박의 70∼80% 발아율종자는 2 Gy 조사에서, 80∼90% 발아율종자는 8 Gy 조사에서 향상되었다. 저선량 조사한 유묘생육은 발아율이 증가한 선량에서 증가하였는데 특히 호박 70∼80% 종자의 2 Gy 조사에서 가장 높았다. 파종 6일 후에 조사한 참박의 효소활성은 4 Gy조사시에 단백질과 catalase 활성이, 호박의 경우는 catalase와 peroxidase 활성이 2 Gy와 8 Gy 조사에서 증가하였다. 참박과 호박종자의 발아와 유묘생육 및 생리활성은 저선량 감마선 조사에 의해 촉진됨을 확인할 수 있었다. To establish the seed treatment method of the gamma radiation for germination enhancement of bottle gourd(Lagenaria siceraria Standl) and pumpkin(Cucurbita ficifolia Bouche), seeds were irradiated with the dose 1~20 Gy of the gamma radiation. The germination rate in irradiated group was higher than that of the control, depending on seed condition. In the case of bottle gourd, the germination rate of 70~80% germinative seed was increased at 4-20 Gy irradiated groups. Especially, it was the highest at 4 Gy irradiated group. The germination rate of 80~90% germinative seed was the highest at 4 Gy irradiated group. The germination rate of 70~80% and 80~90% germinative seed at 4 days after sowing was increased 66% and 26% at 4 Gy irradiated group, respectively. In the case of pumpkin, the germination rate of 70~80% and 80~90% germinative seed was increased at 2 Gy and 8 Gy irradiated group, respectively. Effect of the low-dose gamma radiation on the early growth was enhanced depending on their germination enhancement. Especially, it was the highest at 2 Gy irradiated group of 70~80% gen inative seed of pumpkin. Catalase activity of bottle gourd was increased at 4 Gy irradiated group. Catalase and peroxidase activity of pumpkin were increased at 2 Gy and 8 Gy irradiated group. These results suggest that the germination, early growth and physiological activity of bottle gourd and pumpkin seeds could be promoted by the low dose gamma radiation.

대상 포진 반흔 부위에 발생한 대상 편평 태선 1예

김지숙,김명화,최혜영,명기범 대한피부과학회 2004 大韓皮膚科學會誌 Vol.42 No.2

Many cutaneous reactions have been known to occur within resolved herpes zoster lesions. These include granuloma annulare, pseudolymphoma, lymphoma, granulomatous vasculitis, lichen planus and other dermatoses. The pathogenesis of these reactions remains unclear and it has been proposed that they may represent isotopic response. A dermatomal distribution in lichen planus is very rare. We report here a case of zosteriform lichen planus that was developed on the site previously affected by herpes zoster. (Korean J Dermatol 2004;42(2):217~219)

한천, Sodium Alginate 및 Carrageenan첨가가 유과(부수게)바탕의 품질에 미치는 영향

김중만,전예정,박효숙,송영애,백승화,김명곤 한국식생활문화학회 2005 韓國食生活文化學會誌 Vol.20 No.1

This study was to evaluate effects of agar, sodium alginate and carrageenan on quality of Yugwa(Busuge) base. In the base preparation agar, sodium alginate and carrageenan were added 0.0, 0.1, 0.5, 1.0 and 3.0%(w/w), respectively. Volume, shape, hardness. color(L, a and b value), crude lipid content and sensory evaluation(taste and crispness) of the Yugwa base was measured. Volume of the base was higher than control in case of 0.1~0.5%(w/w) agar, sodium alginate and carrageenan, of which sodium alginate was the highest. Shapes were similar to control. Hardness and crude lipid content was decreased proportional to amount of addition of the three seesweed polysaccharides. the whiteness(L-value) was increased but the yellowness(a-value) and the redness(bvalue) decreased. Taste and crispness were increased in the case of 0.1~1.0%(w/w) of sodium alginate, but agar and carrageenan decreased.

옥수수 뿌리에서 전처리 방법에 따른 굴중성 반응의 효과

김순영,임선화,조명제 안동대학교 기초과학연구소 2000 基礎科學 硏究論文集 Vol.11 No.1

This research were focused on the methods of the pretreatment of chemicals in the study of gravitropic response. The filter paper method which was wrapped the whole roots showed the similar response to the control. According to the methods, we examined the effect of 10(-6) MIAA. The treatment of IAA inhibited the gravitropic response in all the type of pretreatment. Specially, the filter paper method showed the significant effect in the primary roots of maize.

금속촉매산화계에 의한 단백질과 DNA의 산화적 손상에 대한 Candida Protetor의 효과

김명희,김강화 全南大學校家政科學硏究所 1993 生活科學硏究 Vol.3 No.-

Candida pseudotropicalis로부터 분리 정제된 항산화단백은 메르캅탄을 포함한 금속촉매산화계에 의한 대장균 글루타민합성효소의 불활성화 및 효소 단백의 분절을 억제하였을 뿐 아니라 DNA의 분절 또한 억제하였으나 전자공여체로서 아스코르브산을 포함한 금속촉매산화계에 의한 산화적 손상은 억제하지 못하였다. 그러나 아스코르브산을 포함한 금속촉매산화계에 의한 DNA의 분절은 디티오트레이톨 존재시 Candida 항산화단백에 의하여 억제되었는 바 Candida 항산화단백은 금속촉매산화계를 억제하개 위하여 메르캅탄을 요구하는 것으로 보인다.

영아용 가정방문 몬테소리 수행평가 도구 개발에 관한 연구

김명희,신화식,황혜경 한국영유아보육학회 2004 한국영유아보육학 Vol.0 No.39

본 연구는 영아를 대상으로 가정방문 프로그램에서 실시하고 있는 몬테소리 교육 프로 그램에 대한 수행평가도구를 개발하였다. 이를 위해 먼저 가정방문 교사의 수행평가에 대한 전반적인 인식과 요구조사를 거쳐 수행평가도구 개발의 필요성과 중요성을 살펴보았으며, 예비조사와 전문가 협의를 거쳐 도구의 항목과 점수체계를 구성하여 평가 도구를 개발하였다. 수행평가도구는 가정방문 프로그램의 교육내용 중 4개 영역(신체발달, 창의성발달, 일상생활연습, 감각교육)에서 각각 1개씩 총 4개의 수행평가도구를 개발하였다. 개발된 도구는 만 24개월 미만 영아 50명을 대상으로 신뢰도와 타당도를 살펴보았다. 그 결과 매우 높은 문항 내적 합치도를 보여주었으며 구인타당도와 공인타당도를 살펴볼 때 개발된 평가도구는 적절한 측정도구로써 사용될 수 있는 가능성을 나타내었다. The purpose of this study was to develop a Early Childhood Home School Montessori performance Assessment Scale, a scale for assessing the performance of 6-23 month old children in Montessori programs. Subject of the study consisted of 50 6-23 month old children in Seoul and 133 teacher teachers in Seoul. Statistical methods employed were Cronbach's Alpha, construct validity and concurrent validity(with Korean Child Social Maturity Scales). These results indicate that Early Childhood Montessori Home School performance Assessment Scale developed by the authors is a valid scale with high reliability.