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한국산 생약으로부터 해독물질의 개발(제5보) 배양 백서 신경아교세포에서 Caffeic acid와 카드뮴의 결합에 의한 해독효과
백승화,이홍,배현옥,김영옥,곽정숙,유현경,한두석,Baek, Seung-Hwa,Lee, Hong,Pae, Hyun-Ock,Kim, Young-Ok,Kwak, Jung-Suk,Yoo, Hyeon-Gyeong,Han, Du-Seok 한국독성학회 1995 Toxicological Research Vol.11 No.2
This study was carried out to develop the antitoxic compound about cytotoxicity of cadmium on cultured rat neuroglial cells. These cells divided into 3 groups; control group (medium only) or $MTT_{50}$ group (neuroglial cell, $61{\mu}M$ cadmium) and experimental group ($61{\mu}M$ caffeic acid). Neutral red (NR) and tetrazolium MTT of the colorimetric assay were performed to evaluate the cytotoxicity of cell organelles. The light microscopic study was carried out to morphological changes of cultured rat neuroglical cells. The results indicated that caffeic acid showed detoxification effect on cytotoxicity of cadmium in $61{\mu}M$ concentration. According to the spectroscopic study of 1:1 complex of cadmium and caffeic acid, it showed that this formation of complex eliminated cadmium from cultured rat neurogllal cells.
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수산화칼슘[Ca(OH)<sub>2</sub>] 처리 배지를 이용한 느타리버섯의 재배
백승화,이영일,유기용,한성수,Baek, Seung-Hwa,Lee, Yeong-Il,Yoo, Ki-Yong,Han, Seong-Soo 한국환경농학회 2008 한국환경농학회지 Vol.27 No.4
This research is aimed to investigate the cultivation method of oyster mushroom[Pleurotus ostreatus (Jacq. ex Fr.) Kummer] at the non-sterilized medium with $Ca(OH)_2$ treatment. Therefore, experiments were carried out to develop non-sterilization method of medium by addition of $Ca(OH)_2$ for omission of heat sterilization progress of medium. General components, minerals and amino acid in Jiri wild type No. 1 (Pleurotus ostreatus) and production cost were analyzed. For the purpose of omission of heat sterilization progress, treatment ratio of $Ca(OH)_2$ (purity 95%) was 5%(w/w) of dry medium. Initial pH of this medium was 11 and then the pH was changed by 9 after the uniform mixing of the medium for half an hour. The various germs occurred 50% and 100% at pH 8 and pH 7 of the non-sterilized medium, respectively. Production of oyster mushroom increased by $2,030\;ton\;ha^{-1}$ when the main raw material used corn pith instead of waste cotton. The time required of mycelium culture was 30 days when hypha was cultured at the non-sterilized medium, and pinhead occurred when 2 or 3 days was passed after the time required of mycelium culture. Occurrence of pinhead was most rapid at the condition of $22{\sim}26^{\circ}C$, 65% humidity and pH $6.5{\sim}7.0$ and required of $22{\sim}28$ days at $70{\sim}80\;mm$ thickness of non-sterilized medium. Ca content in 1st harvest oyster mushroom was higher than that in 2nd harvest one, and its difference was $30.3\;mg\;kg^{-1}$. Amino acid content by stipe thickness of oyster mushroom was ranged from 411.2 to $343.9\;mg\;kg^{-1}$ both in a pileus and a stipe of 1st harvest mushroom, and from 402.4 to $498.2\;mg\;kg^{-1}$ and from 442.6 to $470.4\;mg\;kg^{-1}$ in those of 2nd harvest one, respectively. The results of the present study suggest that the non-sterilization medium by addition of $Ca(OH)_2$ is usable with the cultivation of oyster mushroom.
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백승화,Nakjeong Kim,Seong Hwan Kim,Kwang Hwa Park,정경채,박배근,강남숙 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.1
Androgen receptor (AR) is ligand-inducible nuclear hormone receptor which has been focused on key molecular target in growth and progression of prostate cancer. We synthesized a series of 4-aryl 2-substituted aniline-thiazole analogs and evaluated their anti-cancer activity in AR-dependent human prostate cancer LNCap cells. Among them, the compound 6 inhibited the tumor growth in LNCap-inoculated xenograft model.
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한국산 생약으로부터 해독물질의 개발(제2보) 흰쥐 간장내의 카드뮴 축적에 미치는 금은화 추출물의 영향
백승화,유일수,이종섭,한두석 한국독성학회 1995 Toxicological Research Vol.11 No.2
This study was conducted to investigate the metallothionein (MT) induction by Lonicerae rios in cadmium chloride intoxication. The results were as follows: Generally, detoxication effects by Lonicerae fios extract increased to the increase of extract concentrations. When 90 mg/kg dosage of Lonicerae fios extract was administered, it showed the highest antitoxic effects in metallothionein induction. From the above results, Lonicerae rios extract increased metallothionein concentration and decreased the toxicity of cadmium in liver.
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한국산 생약으로 부터 해독물질의 개발(제4보) 흰쥐 간장내의 카드뮴 축적에 미치는 인삼 추출물의 영향
백승화,유일수,이종섭,한두석 한국독성학회 1995 Toxicological Research Vol.11 No.2
This study was conducted to investigate the metallothionein (MT) induction by Panax ginseng C. A. Meyer in cadmium chloride intoxication. The results were as follows: Generally, detoxicatlon effects by Panax ginseng C. A. Meyer extract increased proportionally to the increase of cadmium concentrations. When a 8 mg/g dosage of cadmium was administered, formation of the cadmium and EDTA complex showed the highest antitoxic effect. Also, when a 4 mg/g dosage of cadmium was administered, Panax ginseng C. A. Meyer extract showed the highest antitoxic effect in metallothionein induction. According to the above results, Panax ginseng C. A. Meyer extract administered with cadmium increased metallothionein concentration and decreased the toxicity of cadmium in liver.
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