생쥐의 심장발생과정에서 세포증식과 세포고사의 역할

김어진,김효수,서정욱,김어진,김효수,서정욱 대한병리학회 1998 Journal of Pathology and Translational Medicine Vol.32 No.12

인삼첨가 Long Life면의 조직감과 관능적 특성

정재홍,심창주,신영,권효진,이규희,오만진 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.5

인삼이 밀가루의 아밀로그래프에 의한 호화 성질과 파리노그래프에 의한 반죽 성질에 미치는 영향을 조사하였다. 인삼첨가 LL면의 조직감과 관능적 특성에 미치는 영향을 분석하기 위하여 밀가루에 대하여 인삼을 5.0∼10% 첨가하여 LL면을 제조한 뒤 면의 조직 특성 분석 및 조리시험을 하고 관능검사를 하였다. 인삼을 첨가하면 아밀로그래프의 호화개시 온도를 3.1℃ 지연시켰으며, 최고 점도를 40B.U 감소시켰다. 파리노그래프의 흡수율은 1.2% 증가하였으며, 반죽의 안정도는 인삼의 첨가로 2.0분 길게 측정되었다. Hunter L값은 인삼 첨가량이 증가할수록 낮아졌으며, Hunter a, b값은 반대로 측정되었다. 인삼첨가 LL면의 층밀림 압출력은 첨가량의 증가에 따라 높아져 10.0%의 첨가는 대조구 18.65(㎏f)보다 2.3(㎏f) 높은 23.95(㎏f)로 측정되었으며, 경도도 대조구보다 5.4(㎏f) 높게 측정되었다. 인삼첨가 LL면의 무게 증가는 인삼의 첨가량이 증가할 때 다소 높게 나타났으나. 부피 증가는 오히려 높게 나타나 조직이 다소 치밀한 것으로 나타났다 용출량은 인삼량의 증가에 따라 약간 감소하였다. 미생물은 인삼의 첨가량이 증가할수록 줄어들어서 장기 저장이 가능하였다. 관능 검사 결과는 인삼첨가 LL면이 4.2 및 4.0으로 비교적 좋은 점수를 얻었다. 이같이 LL면 제조시 인삼 첨가량은 7.0%의 수준이 효과가 컸다. The influence of ginseng on the paste or gelatinization properties by amylograph and mixing properties by farinograph of wheat flour, and on quality properties, color, cooking quality, textural and sensory properties, and reducing microbial population of LL (Long Life) noodles was studied. The contents of ginseng used were from 5% to 10% based on flour weight. The viscosity property of wheat flour with ginseng was increased the initial pasting temperature but the amylograph peak viscosity were decreased in vice versa. The farinograph absorption, stability and breakdown were increased by ginseng. The whiteness of LL noodles manufactured with ginseng was lower than that of control. The shear extrusion force and hardness of LL noodles manufactured with ginseng were shown much higher value than those of control. At cooking quality examination of LL noodles manufactured with ginseng, weight of cooked LL noodles was decreased but volume was appeared in vice versa. Extraction amounts of LL noodles manufactured with ginseng during cooking were much smaller than those of control. Total counts of microorganism of LL noodles manufactured with ginseng were decreased during storage at 30℃. Sensory properties of cooked LL noodles which was manufactured with ginseng showed quite acceptable. Based on the cooking and sensory evaluation test, addition of 7.0% ginseng to wheat flour may be suitable for processing LL noodles.

인공신경망 이론을 이용한 충주호의 수질예측

정효준,이소진,이홍근 한국환경과학회 2002 한국환경과학회지 Vol.11 No.3

This study was carried out to evaluate the artificial neural network algorithm for water quality forecasting in Chungju lake, north Chungcheong province. Multi-layer perceptron(MLP) was used to train artificial neural networks. MLP was composed of one input layer, two hidden layers and one output layer. Transfer functions of the hidden layer were sigmoid and linear function. The number of node in the hidden layer was decided by trial and error method. It showed that appropriate node number in the hidden layer is 10 for pH training, 15 for DO and BOD, respectively. Reliability index was used to verify for the forecasting power. Considering some outlying data, artificial neural network fitted well between actual water quality data and computed data by artificial neural networks.

Inhibitory Effect of Cordycepin on Human Platelet Aggregation

박화진,--,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1

Cordycepin separated from Cordyceps militaris is a major physiologic active component in Cordyceps militaris. The platelet aggregation is stimulated by Ca^(2+), which is either mobilized from intracellular endoplasmic reticulum or transported from extracellular space. cGMP antagonizes the actions of Ca^(2+). Based on these facts, we have investigated the effects of cordycepin on the mobilization of Ca^(2+) and the production of cGMP on collagen (10 ㎍/ml)-induced human platelet aggregation. Cordycepin potently stimulated the human platelet aggregation induced by collagen (10 ㎍/ml) in a dose-dependent manner. Cordycepin (500 μM) inhibited also the collagen-induced human platelet aggregation in the presence both 1 mM and 2 mM of CaCl_(2). These are in accord with the results that cordycepin inhibited the Ca^(2+)-influx on collagen-induced human platelet aggregation. These results suggest that cordycepin decrease the intracellular Ca^(2+) concentration to inhibit collagen-induced human platelet aggregation. Besides, cordycepin increased the level of cGMP on collagen-induced human platelet aggregation. This result is related with the decrease of intracellular Ca^(2+) concentration, because cGMP inhibits the mobilization of Ca^(2+). In addition, cordycepin inhibited the human platelet aggregation induced by LY-83583, inhibitor of guanylate cyclase. This result suggested that cordycepin inhibit the platelet aggregation by stimulating the activity of guanylate cyclase. In conclusion, we demonstrated that cordycepin might have the antiplatelet function by inhibiting Ca^(2+)-mobilization via the stimulation of the production of cGMP.

셀레콕시브 및 그 합성유도체들의 항암활성 스크리닝

박정란,강진형,구효정,노지영,류형철,박상욱,고동현,조일환,이주영,황다니엘,김인경 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.2

Selective COX (cyclooxygenase)-2 inhibitors including celecoxib have been shown to induce apoptosis and cell cycle changes in various tumor cells. New inhibitors are recently being developed as chemomodulating agents. We evaluated celecoxib and screened 150 synthetic compounds for anti-proliferative activities in vitro. Effects of celecoxib on COX activity, cell growth, cell cycle distribution, and apoptosis induction were determined in A549 COX-2 overexpressing human non-small cell lung cancer (NSCLC) cells. The COX inhibition of celecoxib increased with concentration up to 82% at 1μM after 24 hr exposure. Forty μM and 50μM of celecoxib induce G_1 arrest, and TUNEL-positive apoptotic cells, respectively. Among 150 compounds, several compounds were selected for having greater COX-2 inhibitory activity and higher selectivity than celecoxib with growth inhibitory activity. Celecoxib showed concentration-dependent COX inhibitory activity, and ability to induce cell cycle arrest and apoptosis in human NSCLC cells in vitro. Among synthetic analogues screened, several compounds showed promising in vitro activity as COX-2 inhibitory anticancer agents, which warrant further evaluation in vitro and in vivo.

대장암 주변에서 관찰되는 백색반점의 병리조직학적 특성과 임상적 의의

김효종,조진만,장린,동석호,김윤화,장영운,박일랑,김병호,이정일 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.4

Background/Aims: To investigate the difference between colorectal adenocarcinomas with white spots (foamy cells) and those without white spots, clinically and histopathologically, were examined 37 cases of colorectal adenocarcinomas were classified in this study. Methods: Two groups: those with white spots and those without. In each case, evidence of lymph node and liver metastasis was sought. Immunoreactive staining for macrophage was performed. Results: Increased incidence of exophytic tumors was found (73%, p=0.028) in the group with white spots. The incidences of lymph node metastasis were 33% vs. 45% between the group with white spots and the group of without, but the difference was not statistically significant. Conclusions: These results suggested that foamy cells might have a beneficial role in colorectal cancer, although they were not statistically significant. Therefore, further prospective study be warranted.

矢狀分割骨切斷術에 依한 顎矯正術의 統計學的 硏究

辛曉根,陳宇政,李俊禮,金晤煥,李炫尙 大韓顎顔面成形再建外科學會 1997 Maxillofacial Plastic Reconstructive Surgery Vol.19 No.2

Recently, combined with social complexity, increment of medical demand and supply and the change of esthetic category, admission and operation of the patients of facial deformity have been changed with annual change. This study was conducted in the concept of helping the overall character of orthognathic surgery in future understood and being an important index in the establishment of better treatment course, through the patient, on whom was operated orthognathic surgery via sagittal split ramal osteotomy in our Dept. of Oral & Maxillofacial Surgery, Chonbuk National University Hospital from 1991. 1. 1 to 1995. 12. 31. The results were obtained as follows. 1. The ratio of male to female was 1 : 1.33 and the ages between 16 and 25 year was 73.6%, and the mean age was 20.4 years. 2. The ratio of setback amount between 6 to 15mm was 84.6% and the advancement amount between 1 to 10mm was 89% and the mean amount of movement was 9.0mm in setback, and 3.6mm in advancement. 3. After removal of maxillomandibular fixation(MMF), the distinction of sex was not statistically significant in ROM. 4. The ROM following methods of fixation was statistically significant in 3rd(P<0.05), 8th, and 12th week(each P<0.01), with faster rehabilitation in rigid fixation which had shorter MMF period. 5. The rehabilitation of ROM following the operation methods was statistically significant in 1st(P<0.05), 2nd, 3rd, 4th, 6th, 8th, and 12th week(each P<0.01) with faster rehabilitation in the case of SSRO than Lefort I / SSRO (Two jaw surgery). 6. The rehabilitation of ROM following directions of mandibular movement did not manifest a statistically significant difference in both setback and advancement.

복강경을 이용한 부인과 수술의 임상적 고찰

조혜진,정효영,정혁 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Background and Objectives : To assess the advantages, disadvantages, appilcability, and complications of laparoscopy in gynecologic disease. Materials and Method: A retrospective study which includes 591 patients who underwent laparoscopic surgery from January, 1994 to February, 2005 for gynecologic diseases was done. Results: The patient's ages ranged from 19 to 65 years and their mean age was 40years. The indications of laparoscopy were myoma in 240 cases (40.6%), ovarian tumor, ectopic pregnancy, pelvic adhesion, primary infertility, secondary infertility, endometriosis, pelvic inflammatory disease, hemorrhagic corpus luteum and tubal obstruction in decreasing order. The operations performed were laparoscopic-assisted vaginal hysterectomy in 247 cases (41.8%), diagnostic laparoscopy, oophorectomy, salpingo-oophorectorny, ovarian cystectomy, pelvic adhesiolysis, salpingectomy, ovarian wedge resection and fulgarization in order. Operation times were from 10 to 240 minutes (mean 64 minutes). Postoperative hospital stays were from 0 day to 15 days (mean 3.9 days). There were 11 surgical complications including 1 trocar site hematoma, 11 incision site infection, 2 postoperative ileus and 1 patient underwent reoperation because of delayed ureteral fistula. Conclusion: We conclude that operative laparoscopy is useful in gynecologic diseases but need development of laparoscopic equipments, research of operative method, extension of operative indications, and skilled laparoscopic surgeons.

HMG-CoA 환원효소 억제제에 의한 ICAM-1 유전자의 발현조절

김현진,정효균,홍우정,김군순,조영석,김도희,채수흥,구본정,송민호,노흥규,김영건 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : ICAM-1 act as one of major adhesion molecules in the atherosclerotic lesion. ICAM-1 expression is mainly regulated at the level of transcription and depend on IFN-γ signal transduction pathway in which the STAT1 transcrption factor is a critical intermediate. IFN-γreceptor not only initiates tyrosine 701 phosphorylation of STAT1 by Jak1 and Jak2, but also phosphorylates serine 727 through the activation of Raf-1/MAP kinases. HMG-CoA reductase inhibitors have anti-atherosclertic effects, beyond normalization of hypercholesterolemia, by directly acting on endothelial cells, macrophages and vascular smooth muscle cells. HMG-CoA reductase inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibition posttranslational farnesylation and geranyl-geranylation processing of small GTP-binding preoteins and inhibition of normal signaling activities. Method : We made several 5'-deletion constructs of rat ICAM-1 promoter and analyzed the promoter activities by measuring the luciferase activity after transfection into ECV304 cells and smooth muscle cells. We checked the level of total and phosphorylated STAT1 protein by immunoblot analysis using specific antibodies. Results : Lovastatin inhibits IFN-γ-induced ICAM-1 gene expression in the ECV304cell. The cells pretreated with PD98059, MEKK inhibitor showed significantly low ICAM-1 RNA induction with IFN-γ stimulatio. IFN-γ induced phosphorylation of tyrosine 701 was not significantly changed by the pretreatment of lovastatin. But lovastatin suppresses IFN-γ-induced phosphorylation of ERK1/ERK2 which are responsible for the seine 727 phosphorylation in STAT1. Conclusion : We showed that HMG-CoA reductase inhibitors, lovastatin, suppresses IFN-γ mediated ICAM-1 gene expression through the inhibition of transcription. HMG-CoA reductase inhibitor suppresses IFN-γ-induced phosphorylation of serine 727 in STAT1 through the modulation of MAP kinases.

천추 갈색종양(Brown tumor)으로 발현된 부갑상선 기능항진증 1예

홍우정,구본정,이재민,한상우,이효진,나소영,노흥규,김영건,송민호 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

부갑상선 기능항진증에 의한 골병변으로 갈색종양이 있다. 갈색종양은 주로 장골을 침범하고 척추골에서의 발생은 매우 드물다. 국내의 보고에서 갈색종양의 장골 침범은 있었으나, 척추골에서의 발생은 보고된바 없다 .저자들은 부갑상선 기능항진증으로 인하여 척추골의 천추에 발생된 갈색종양 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. The bony involvement in primary hyperparathyroidism present as browm tumor or osteitis fibrosa cystica and lead to pathologic fracture. Brown tumor commonly involve long bone such as femur, tibia, fibula, humerus, and radius. Brown tumor of axial skeleton is a rare entity. Previously reported case that involve axial skeleton still not present. We report a 47-year-old male patient that he has a hyperparathyroidism presenting as brown tumor involving sacrum.