칡뿌리의 항산화 성분

오만진,이가순,손화영,김성렬 충남대학교 농업과학연구소 1990 농업과학연구 Vol.17 No.1

As a search for natural antioxidants, antioxdative fractions in Pueraria roots were extracted, identified using column chromatography, thin layer chromatography or high performance liquid chromatography. The components which have most effective antioxidative activities were further identified by IR and GC-MS. Separated antioxidative components were then added to four different oils to examine their antioxidative activities. Yield of extract obtained from pueraria root powder by solvent extraction using four step solvent systems was 2.54%. Antioxidative activity of the extracts was as effective as that of 100 ppm δ-tocopherol addition, when 0.1% of the extracts was added to linoleic acid. The strongest antioxidative component of methanol extract of pueraria root was identified as puerarin. Antioxidative activity of puerarin on lard was more effective than α-tocopherol, but less effective than δ-tocopherol. When the puerarin was added to edible oil and heat treated at 145℃, the acid value was lowest in lard and was highest in soybean oil. Antioxidative activity in terms of carbonyl value, thiobarbituric acid value and anisidine value was most high in palm oil and least in soybean and cottonseed oil.

여천공업단지에서 배출되는 이산화유황이 느타리버섯의 생육에 미치는 영향

오만진,신관철 한국균학회 1991 韓國菌學會誌 Vol.19 No.4

Sulfur dioxide discharged from the factories in Yeochun industrial complex severely damaged to the growth of oyster mushrooms. The continuous monitoring indicated that high concentration of sulfur dioxide was repeatedly detected in Kwang-Yang and Seung-Ju. The first injury symptoms on the mushroom fruit bodies were observed a day later when sulfur dioxide was detected over 300 ppb. Under the high concentration of sulfur dioxide exposure, the young fruit bodies showed blight and sudden death and the mature fruit bodies turned to blue-brown and frequently progressed to blight. The symptoms at Spring cropping were more severe than those at Autumn cropping. The severity was associated with the frequency of sulfur dioxide monitored. However, sulfur content in the mushroom fruit bodies and culture media did not show clear differences between the injured mushroom from Yeochun industrial complex areas and normal ones from no damaged areas.

고온성세균의 $\beta$-Galactosidase에 관한 연구( II )-효소의 생산, 정제 및 정제효소의 성질-

오만진,이종수,김해중,김찬조 한국미생물 · 생명공학회 1983 한국미생물·생명공학회지 Vol.11 No.1

전보에서 분리 동정한 Y-33 균주의 $\beta$-galactosise 효소생산조건을 검토하고 효소를 정제하여 정제효소의 성질을 조사한 결과는 다음과 같다. 1. 효소생산을 위한 최적초발 pH는 7.0이었고 최적온도는 $65^{\circ}C$이었다. 2. 효소는 lactose와 galactose에 의하여 유도되어졌으며 세포내효소이었다. 3. 조효소액을 1차 DEAE-cellulose, 2차 DEAE-cellulose column chromatography 및 Sephadex G-150로 gel filtration하여 정제도가 28.3배, 수율이 15.2%의 정제효소를 얻었다. 4. 정제효소는 polyacrylamide gel 전기 영동에 의하여 순도가 검정되었다. 5. 정제효소의 유당가수분해를 위한 최적작용 온도는 $65^{\circ}C$. pH는 6.5이었다. This experiment was carried out to optimize the condition for the enzyme production by selected strain in the basal medium, to purify the enzyme and to characterize the purified enzyme. The results obtained were as follows. 1. The optimal conditions for the $\beta$-galactosidase production were initial pH 7.0 and temperature $65^{\circ}C$. 2. Enzyme was induced by the addition of lactose and galactose, and it was intracellular enzyme. 3. The purified enzyme was obtained with the increased level of activity approximately 28.5 folds as compared with crude enzyme and the yield of 15.2% by means of DEAE-Cellulose column chromatography, Sephadex G-150 gel filtration 4. $\beta$-galactosidase from final step of purification showed a sing1e protein band on polyacrylamide gel disc electrophoresis. 5. The optimal temperature and pH of the purified enzyme were $65^{\circ}C$, pH 6.5 for the hydrolysis of lactose.

섬유 복합재료 경계면에 대한 그래핀/카본 나노튜브 하이브리드 코팅의 효과 연구

오만진 한국기계기술학회 2020 한국기계기술학회지 Vol.22 No.6

In this research, carbon nanotubes(CNT) and graphene nanoplates(GnP) are deposited on the surface of carbon fibers(CF) at once. Investigating the effect between CNT and GnP on increasing the interfacial and mechanical properties of carbon fiber reinforced epoxy composites(CFRP). The cross section of the CFRP composites indicates that the GnPs/CNTs hybrid coating exhibits significantly higher mechanical performance in all coating samples. The interlayer shear strength of the GnPs/CNT hybrid coated CFRP composite was 90% higher than that of the uncoated CF composite. The flexural and tensile strength of CFRP composites using GnPs /CNT hybrid coatings were improved by 52% and 70%, respectively, compared to uncoated CF.

대두 α - galactosidase 의 정제 및 성질

오만진,금종화,김성렬 한국농화학회 1991 Applied Biological Chemistry (Appl Biol Chem) Vol.34 No.3

To elucidate enzymatic properties of α-galactosidase (EC 3, 2, 1, 22) from germinated soybean, changes in the enzyme activities and oligosaccharide contents during germination of soybean were determined. α-Galactosidase from germinated soybean was purified by ammonium sulfate fractionation, ion exchange chromatography and gel filtration. Their chemical and enzymatic properties was investigated. α-galactosidase activity of sobeam was maximized when it was germinated at 25℃ for 120 hour. Raffinose and stachyose in soybean were decomposed completely after 96 hours and 120 hours of germination, respectively. Soybean α-galactosidase was purified by 6.6 fold by ammonium sulfate fractionation, ion exchange chromatography on DEAE-Cellulose and Sephadex A-50, and gel filtration on Sephadex G-150. Its specific activity was 825 Units/㎎ protein and the yield was 2.5% of the total activity of crude extracts. The purified α-galactosidase of soybean was found to be homogeneous by polyacrylamide gel electrophoresis and by HPLC. Isoelectric point of soybean α-galactosidase was determined analytical isoelectric focusing to be pH 4.8. The soybean α-galactosidase was monomeric and its molecular weight was estimated to be 30,000 by SDS-PAGE. The optimal temperature and pH for the soybeam α-galactosidase activity were 40℃ and pH 6.0 and 75% of its activity was lost by heating at 60℃ for 10 min. The enzyme was appeared to have higher affinity to raffinose than to stachyose. The Km value of soybean enzyme was 5.3 mM for ρ-nitrophenyl-α-D-galactopyranoside and the activation energy on PNPG was calculated to be 13.02 K㎈ per mole.

紅景天(Rhodiola sachlinensis)에서 향균성 물질의 분리 및 동정

오만진,이상덕,정재홍,이규희,김영호,심창주 한국식품저장유통학회 2004 한국식품저장유통학회지 Vol.11 No.1

홍경천의 천연 보존료로서의 이용성을 검토하기 위하여 각종 용매로 홍경천 추출물을 제조하고 극성에 따라 순차 분획, silica-gel chromatography, thin-layer chromatography, high performance liquid chromatography의 분리과정을 거쳐 순수 분리된 각 물질을 GC/MS(EI) spectrum, 1H-NMR spectrum, 13C-NMR spectrum을 이용하여 항균물질을 동정하여 다음과 같은 결과를 얻었다.홍경천에 대해 acetone, ethyl acetate, ethanol, methanol, chloroform, water의 각 용매로 추출한 결과 항균성분의 추출용매로는 methanol이 가장 우수하였다. 홍경천 methanol 추출물을 n-hexane, chloroform, ethyl ether, ethyl acetate, n-butyl alcohol로 분획한 결과 ethyl acetate와 n-butyl alcohol 분획에서 우수한 항균력을 나타내었다. 홍경천 methanol 추출물은 그람양성 4균주와 그람음성 4균주에 대해 고체 배지의 경우 대체로 500 μg/mL에서 최소저해를 나타냈으며, 액체배지의 경우 100 μg/mL 내외에서 최소저해를 나타내었다. Silica gel column chromatography에서 용매의 비율을 높여가며 용출한 결과 chloroform : methanol(8:2, v/v)의 비율에서 항균력이 가장 높게 나타났다. 분리된 항균성분을 silica-gel TLC plate에 용매의 비율을 높여가며 전개시켜 chloroform : methanol(9:1, v/v)에서 9개의 물질군을 얻을 수 있었으며, 그 중 항균력이 확인된 3개의 물질군을 HPLC와 NMR spectrum을 분석한 결과 gallic acid, (-)-epicatechin 및 kaempferol로 동정되었다. gallic acid, (-)-epicatechin, kaempferol의 항균물질 각각의 최소저해농도는 Staphylococcus aureus와 Listeria monocytogenes 에 대해 gallic acid와 kaempferol이 500 μg/ mL, (-)-epicatechin이 100 μg/mL에서 저해를 나타내었다.

폐기종실(廢棄種實)의 식량자원화에 관하여 : 제3보 : 호박 및 참외 종실(種實)의 화학적 조성 Part Ⅲ. Chemical composition of Pumpkin and Melon Seeds

오만진,윤형식,최청 한국농화학회 1983 Applied Biological Chemistry (Appl Biol Chem) Vol.26 No.3

A study was conducted to find out the possibility of utilizing cucurbit seeds as food resources of fats and proteins. The pumpkin and melon seeds contained about 41% of crude fat and about 31% of crude protein. The lipid fractions obtained by silicic acid column chromatography were mainly composed of about 96% neutral lipid, and among the neutral lipid separated by thin layer chromatography, triglyceride and free fatty and were major components in both seeds. The predominant fatty acids of the pumpkin and melon seeds were linoleic acid(51.8∼70.5%), palmitic acid(11.0∼27.8%) and oleic acid(7.8∼16.4%). The salt soluble proteins of the cucurbit seeds were highly dispersible in 0.02M sodium phosphate buffer containing about 1.0M MgSO₄ salt, and the extractabilities of the pumpkin and melon seed proteins were 40% and 32%, respectively. Glutamic acid, arginine and tyrosine were the major amino acids, and the essential amino acids such as lysine, threonine, valine, methionine, isoleucine, leucine and phenylalanine were detected in both seeds. The electrophoretic analysis showed 6 bands in pumpkin seed protein and 8 bands in melon seed, and the collection rate of the main protein fractions purified by sephadex G-100 and G-200 were 58.3% in pumpkin seed and 55.0% in melon seed. Arginine and glutamic acid were the major amino acids in the main seed proteins and the molecular weight for the ruin proteins of the pumpkin and melon seeds were estimated to be 48,000 and 78,000, respectively.

칡 뿌리의 Polyphenol Oxidase 의 정제 및 성질에 관한 연구

오만진,이원용,이가순 한국농화학회 1988 Applied Biological Chemistry (Appl Biol Chem) Vol.31 No.4

Acetone powder was prepared from raw arrowroots and the polyphenol oxidases of crude enzyme prepared from acetone powder were identified 5 isoenzymes by staining with catechol containing 0.05% phenylene diamine. The crude enzyme was passed through the columns of ion exchangers and gel permeation to fractionate the polyphenol oxidases. The main fraction of polyphenol oxidase appeared to be purified by 94-fold, with the activity yield of 45.4%, and its molecular weight was determined as 38,500 by poly acrylamide gel electrophoresis. The optimal pH and temperature for the enzyme activity were pH 7.5 and 50℃, respectively. The purified enzyme showed a high affinity for catechol and pyrogallol. The Michaelis constant for catechol was calculated to be 16. 67mM according to the Lineweaver-Burk method. The enzyme activity was strongly inhibited by L-ascorbic acid, sodium bisulfite, EDTA and KCN, and totally inhibited, by Fe^(3+) at a concentration of 1mM. However the enzyme was activated by Zn^(2+) approximately 1.7 times at the same concentration.

Humicola sp.의 酵母細胞壁 分解酵素에 관한 연구

吳萬鎭 충남대학교 대학원 1977 論文集 Vol.9 No.-

A number of cell wall lytic fungi, capable of growing and forming clear zone around the colonies on the baker's yeast agar medium, were isolated from various soils and sewages. The stsain M-10, which was identified as a Humicola sp. according to the manual of The Fungi by Ainsworth(1973) appeared to be the most powerful strain among 386 isolates. Detailed experiments were carried out to optimize the conditions for the enzyme production by this strain in the medium containing 5% of baker's yeast, 0.3% of K₂HPO₄, 0.01% of MgSO₄, 7H₂O, 0.1% of yeast extract, and to characterized the purified enzyme obtained from the culture filtrate. Cultivation was done on a reciprocal shaker using baker's yeast mineral as basal in flasks. The results obtained were as follows: 1) The optimal conditions for lytic enzyme production were: initial pH of the medis 6.0, and temperature 33℃. 2) Among the various carban sources, baker's yeast as the concentration of 4% was the most effective for the enzyme production. Monosaccharides supported the increase in cell mass production but repressed the enzyme level as low as approximately one-tenth of those produced on baker's yeast. 3) Baker's yeast was found to be the most effective nitrogen sources for the enzyme production, whereas the other nitrogen sources supported the enzyme production with much lower level than baker's yeast did. The addition of NH₄NO₃, (NH₄)₂ SO₄, or peptone to the medium containing baker's yeast increased the enzyme level slightly but (NH)₄CO, NaNO₃, milk casein or nutrient broth resulted a made repression of the enzyme production. Baker's yeast appeared to supply enough nitrogen and carbon substances for the enzyme production without further supplements. 4) Maximum yield of the lytic enzyme was obtained by addition of K₂HPO₄ and MgSO₄ 7H₂O, at the concentration of 0.1% and 0.01% respectively, to the basal medium among various inorganic salts tested. 5) The best medium for the lytic enzyme production by the strain was found to consist of 4% of baker's yeast, 0.1% of NH₄NO₃, 0.1% of K₂HPO₄, 0.01% of MgSO₄, 7H₂O and 0.1% of yeast extracts at pH 6. 6) When the strain was cultured under the optimal conditions, the production of the lytic enzyme was increased and reached maximum level in 72 hrs. in shaking flasks. 7) The purified enzyme was obtained with the increased level of the 1ytic activity approximately 21.4 folds as compared with the orginal and the yield of 14.6% throught sequencial treatments of the culture filtrates by means of salting out with (NH₄)₂SO₄ (0.3-0.5 saturation), desalting by electric dialyzer, precipitating in acetone (30%-60%) and fractionating by the DEAE-cellulose column chromatography. 8) The purified enzyme appeared to have the optimal pH 4.0 and to be stable in the range of pH 3.5-6.0, however, it lost about 27% of the lytic activity of pH 7.0 and 53% at pH 2.0 during standing it at 15℃ for 12 hours. 9) Optimal temperature of the purified enzyme was 45℃ for the 1ytic action, and it was comparatively stable up to 55℃ and inactivated markedly above 70℃ in 60 minutes. 10) Glucan, laminarin and heat treated baker's yeast were hydrolysed rapidly but living baker's yeast was not degraded by the purified enzyme. 11) The purified enzyme of the strain was believed to be a β-1, 3-glucanase, and was capable of hydrolysing heat-treated baker's yeast alone. 12) The purified enzyme was activated by Mg^(2+) and Co^(2+) but strongly inhibited by Hg^(2+) and Cu^(2+).

충청지역 농·수산물의 잔류농약 및 중금속 함량에 관한 조사

오준세,이석주,성창근,김성애,정재홍,오만진 忠南大學校 環境問題硏究所 1998 環境硏究 Vol.16 No.-

This studies were performed to analyze the amount of heavy metals and pesticide residues from fifty five different samples which produced and distributed in Chungcheong area from January 1997 to December 1998. This investigation will provide the valuable information as to how much regional agricultural products are contaminated from the various pollution sources. The presence of the pesticide residues was absolutely ignorable to below the maximum permissible limit. Rice, pepper, strawberry, apples, and grapes in various agricultural products showed high pesticide detection rate. BHC, captan, endosulfan, EPN, chloropyrifis, and diazinon were detected with relatively high frequency compare to others. The amount of heavy metal in fish and clam catched and distributed at the Chungcheong region were a just natural level, meaning that contamination in the fishery products was not serious.