비혈연간 동종골수이식에 의해 치료된 중증 재생불량성빈혈 1예

김현중,이홍기,이방훈,이창근,이종태,정현식,김원석,윤성수,강원기,박근칠,김대식,고영혜,박찬형 대한조혈모세포이식학회 1997 대한조혈모세포이식학회지 Vol.2 No.1

비혈연간 동종골수이식은 혈연간 동종골수이식이 불가능한 환자에서 기존의 면역역제요법으로 효과가 없었던 경우나 재발한 경우에 적응증이 될수 있다. 이식 거부와 이식편대숙주병의 발생이 골수 이식의 성공률을 감소시키므로, 보다 강력한 conditioning regimen의 사용과 T cell이 제거된 골수를 사용하는 것이 필요하다. 기존의 high dose corticosteroid나 cyclosporine에 대하여 불응성인 이식편대숙주병의 치료를 위해서 ATG와 Mycophenolate mofetil (Cellcept®) 의 사용이나 Tacrolimus, Methotrexate와 같은 다른 면역억제제치료를 고려해 볼 수 있다. It is known that allogenic bone marrow transplantation is the most effective treatment for aplastic anemia. However, this treatment is only applicable to a minority of patients because the proportion of patients who have HLA-matched siblings about 25% to 30% of all the aplastic anemia patients. In the case of the absence of HLA-matched donor, unrelated allogenic bone marrow transplantation is considered. However, (because of severe acute and chronic GVHD and graft failure) the success rate of this approach is only 20~30%. Recently, it was reported that increased immunosuppressive condition combined with T cell depletion of the marrow graft would result in greater success. There was no case of successfully treated aplastic anemia patient by unrelated allogenic bone marrow transplantation in Korea. Here, we report a case of severe aplastic anemia successfully treated with unrelated allogenic bone marrow transplantation.

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

장기간 Risperidone 투여후 지연성으로 발생한 Neuroleptic Malignant Syndrome 1례

이헌정,조방현,김인,이민수 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.4

Risperidone은 비교적 최근에 개발된 항정신병약물이며, 그것이 가진 도파민 D2 수용체와 세로토닌2 수용체를 차단하는 작용기전, 추체외로 부작용과 지연성 운동장애가 적다는 점, 정신분열병의 음성증상에도 효과가 있다는 점에서 흔히 비전형적 약물로 불리운다. 그러나 이러한 비전형성에도 불구하고, 이미 전세계적으로는 15개 정도의 risperidone에 의한 신경이완제 악성증후군(neuroleptic malignant syndrome : NMS)의 보고가 있으며. 국내에서도 최근 하나의 증례가 보고되었다. 저자들은 정신분열병으로 장기간 risperidone으로 치료받던 젊은 남자 환자에서 치료 1년 9개월만에 발생된 NMS 1례를 경험하였기에 관련문헌의 고찰과 더불어 증례보고하고자 한다. Risperidone is a relatively new antipsychotic agent. It is often referred to as 'atypical', because it has a mechanism of action that blocks post synaptic dopamine-2 and serotonin-2 receptors, it is associated with fewer extrapyramidal side effects, it is not yet associated with tardive dyskinesia, and it may have some efficacy on negative symptoms of schizophrenia. In despite of its 'atypical' nature, there are already more than 15 reports of risperidone-induced neuroleptic malignant syndrome. Only one case of risperidone-induced NMS was reported recently in Korea. We report one case of delayed risperidone-induced neuroleptic malignant syndrome in young male patient and review the related articles.

고등학생의 인터넷 사용정도와 학교적응, 친구관계에 대한 연구

이현주,김미연,이희재,권소영,김민정,김민주,방은혜,이경희,김수지,이자형 이화여자대학교 간호과학대학 2004 이화간호학회지 Vol.- No.38

Today, almost all families are quipped with computers. At the same time, we can find lots of side-effects because of computers. If young students, who tend to be unable to control their impulses once they are immersed in internet, the problem will be more serious. It could be happened that the young students can't distinguish the real from the image. Therefore, it is important to grasp the actual condition of juvenile Internet addicts and their practical life, and to come up with basic materials necessary for working out countermeasures. This study surveyed 339 high school students from three high schools in the area of Seoul. Data collection were collected from 22nd of December, 2003 to 27th of December 2003. The collected data were analyzed by SPSS program using T-test, ANAVA and Pearson correlation coefficient. The results were as follows: First, of the 333 respondents 9.61% of them were high-risk internet using group, 15.02% of them were potential-risk internet using group. 25% of then were identified as addicted group. Second, the more addicted the subjects are, the lower their level of school adjustment becomes. For the addicted group, it is difficult to adjust their school and to have a good relationship with friend. Third, the more addicted the subjects are, the lower their level of friendship becomes. The subordinate areas of friendship have same results as the level of Internet addiction and the extent of friendship. If students are addicted to Internet, it is difficult to find friends who can give them encouragement in practical life. Firth, there was a negative relationship between Internet addiction and school adjustment, and, there was a negative relationship between Internet addiction and friendship. Through this study we have seized the relationship between Internet addiction and school adjustment and friendship. As a result, the more addicted, the more difficult to adjust to school. And for the students who were addicted, it is difficult to make a friend.

광주기에 따른 참전복의 성장

이현중,서총현,이상민,박상용,방인철 순천향대학교 기초과학연구소 2003 순천향자연과학연구 논문집 Vol.9 No.2

A 73-days rearing trial was conducted to investigate the effects of different photoperiod on growth and survival rate of juvenile abalone (Nordotis discus hannai). Survival rate was not significantly affected by different photoperiod condition (P>0.05). Growth rates on shell length and shell breadth of 12L/12D, 18L/6D and 24L/0D groups were higher than those of control (P<0.05), but growth on shell length, shell breadth and shell height of 0L/24D group was lower than those of control (P<0.05). Soft body weight and fatness index of abalone reared 24L/0D condition was greater than all of experimental groups. The results suggest that long photoperiod can improve weight gain of abalone

의류상품의 특성과 문제점 개선안에 관한 연구

방경혜,송민재,이영해,조현애,최문희 연세대학교 생활과학대학 1985 婦學 Vol.18 No.-

Nowdays, proper merchandising system of clothing is needed not only for functional clothes but also for fashionable ones. Since nineteen eighties, ready­to­wear garments have been popular for Korean women. But it is hard to tell whether or not the system of the ready­to­wear industry is concrete because of the short history. The purpose of this study was to investigate the process of the merchandising system of the ready­to­wear garments and to recognize the problem which the system of the garments had have. Furthermore, it aimed to find out the idea or improving the problem for better ready­to­wear garments. This research was done through information obtained from several libraries. And, the period of this was from May of August, 1984. The results of this study were: 1. There was an indivisible relationship between merchandising system and fashion, and the importance of the merchandising­planning was raised. 2. The manufacturers should research and analyze consumers since they are aiming at them in nature. 3. The manufacturers should aim at the invention of the creative designs and the introduction of advanced techniques in producing textiles and their accessaries. 4. There should be some institutes to teach fashion and other areas related to ready­to­wear garments. 5. It was necessary to inform consumers to be able to select proper garments in the market.

수면무호흡증이 혈압에 미치는 영향

이희상,이성훈,조현상,남궁기,한승진,방승규 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.1

본 연구에서는 수면무호흡증이 혈압에 미치는 영향을 살펴보고자 하였다. 연구대상은 1995년 1월1일부터 1995년 10월 31일까지 코골기와 수면무호흡 증상으로 인해 연정 뇌기능 수면 연구소에 수면다원검사가 의뢰된 140명의 환자를 대상으로 하였으며 그 중에서 원발성 고혈압을 제외한 심혈관계 질환이 있거나 항고혈압제를 복용하고 있는 환자, 그리고 자료가 불충분하거나 수면무호흡증으로 진단되지 않은 24명을 제외하고 나머지 116명을 최종분석 대상으로 하였다. 이들 대상 환자들로부터 얻은 자료를 수면변수와 호흡변수로 나누어 혈압과의 관계를 Pearson 상관관계와 다중회귀분석을 통해 다음과 같은 결과를 얻었다. 1) 평균 수축기 및 확장기 혈압과 유의한 상관관계가 있는 변수는 수면무호흡의 총시간 및 총횟수, 총수면시간중 평균 혈중산소포화도, 무호흡지수, 중추성 수면무호흡의 총시간 및 횟수, 폐쇄성 수면무호흡의 총시간등 이었다. 2) 다중회귀분석 결과 평균 수축기 및 확장기 혈압에 유의한 영향을 미치는 것으로 나타난 변인은 수면무호흡의 총시간이었으며 이것이 평균 수축기 및 확장기 혈압에 미치는 영향의 도는 각각 7.9%와 6.2%였다. 이상의 결과로 볼 때, 수면무호흡증이 혈압에 미치는 영향은 적은 것으로 나타났으며 무호흡의 유형에 관계없이 수면무호흡의 총시간만이 혈압에 영향을 주는 것으로 나타났다. 그리고 중추성 수면무호흡의 경우 평균 혈중 산소포화도의 감소, 각성에 따른 교감신경계 활성도의 증가이외에도 다른 기전에 의해 혈압 상승이 나타날 가능성이 있을 것으로 생각된다. Objects : This study was conducted to investigate the effects of sleep apnea syndromes on th systemic blood pressure. Methods : The subjects were 140 patients who were consulted to Yon-jung brain function and sleep research center for polysomnography due to snoring and sleep apnea from Jan. 1st 1995 to Oct. 31st 1995. Twenty-four out of 140 were excluded due to cardiovascular diseases other than systemic hypertension, antihypertensive medication, inadequate data or diagnosis other than sleep apnea syndrome. The remaining 116 were accepted as proper subjects. Using Pearson's correlation and multiple regression analysis, we looked for the relationship between sleep and respiratory variables to systemic blood pressure. Results : The results were as follows : 1) According to the correlation analysis, statistically significant variables to mean systolic and diastolic blood pressure were the total time and total number of sleep apnea, degree of oxygen saturation of total sleep time, apnea index, total time and total number of central apnea, and the total time of obstructive apnea. 2) According to the multiple regression analysis, only the total time of sleep apnea significantly affected mean systolic and diastolic blood pressure, and the degrees of affection were 7.9% and 6.2% respectively. Conclusion : these findings showed that sleep apnea syndrome had lower influence over systemic blood pressure than expected and regardless of the type, only the total time of sleep apnea influenced systemic blood pressure. In the case of central apnea, mechanisms other than hypoxemia and the increase of sympathetic activity during arousal may make it possible to increase systemic blood pressure.

저·고온 환경노출이 혈 중 Triglyceride, Glucose의 농도와 Mitochondria의 크기에 미치는 영향

이언호,방현석 부산 외국어 대학교 2002 外大論叢 Vol.24 No.-

This of study was utilized for in order to know Mitochondria size gestrogen, liver, heart of white rat 30 heads, to know triglyceride and glucose concentration cold warm environment exposure. A sample of test is on the warm control 10 heads, cold environment is 10 heads about 4 weeks, after cold environment is no relax, but on the warm situation condition breed 10 heads about 4 weeks. Test is separate 10 heads Each organ analysis with chemical treat and Electronic microscope. The result of study is difference glucose color of blood show cold, control<warm after cold and difference triglyceride concentration of bloods show cold<warm after cold·control also me measure mitochondria size in order to length and wide on the cold situation, breeded rat as grouth mitochonria size. The warm after cold sithatim is not difference control. The summary of result, control the temperature is much effect to triglyceride and glucose concertration, to mitochondria size of muscle, heart, liver.

양파육질과 양파껍질의 용매별 추출물이 항산화 효과에 미치는 영향

이재민,방현아,조정순 명지대학교 자연과학연구소 1995 자연과학논문집 Vol.12 No.-

본연구는 양파를 앙파껍질(onion peel, OP)과 양파육질(onion flesh, OF)로 구분하여 항산화늄이 높은 최적의 용매를 선정하기 위하여 용매종류를 달리하여 추출액을 조제한 다음 추출수율,DPPF(1,1-diphenyl-2-pycryl hydrazyl)에 의한 수소공여능을 측정하고 linoleic acid를 model system으로 하여 과산화물가, 과산화지질 형성억제능을 측정한 항산화 효과를 보였고 과산화지질 형성억제능은 양파육질의 경우 70% 에탄올군에서 높은 항산화 효과를 나타냈다. Onion Flash(OF) and yellow onion peel (OP) were extracted with 5 solvents in order to select optimal extraction solvents. And they were studied for the effects of antioxidant activity by measuring the extraction yields, eletron donating abilities and the inhibitory effects on lipid peroxidation of linoleic acid by using the method of thiobarbituric acid(TBA)and thiocyanate. Electron donating abilities were the highest level of activities in 70% ethanol extract in case of OP and OF. And the inhibitery effects on lipid peroxidation (by using the method of TBA and thiocyante)were the hightest in 70% ethanol extract in case of OF.

전신마취유도 중 발생한 심방세동과 회복중 발생한 발작성 상심실성 빈맥

방윤식,신동욱,이태규,박정현,길현주,이종연 이화여자대학교 의과학연구소 2012 EMJ (Ewha medical journal) Vol.35 No.2

A healthy 35-year-old man who was scheduled for closed reduction of nasal bone fracture developed atrial fibrillation during induction of general anesthesia after intravenous glycopyrrolate injection. During emergence of general anesthesia, atrial fibrillation was suddenly changed to paroxysmal supraventricular tachycardia with 200 beat per minute and lasted for about 10 seconds. Because blood pressure was stable, esmolol was used to reduce ventricular response. At recovery room, ventricular response reduction about 55 beat per minute was observed after intravenous injection of verapamil 5 mg. Thereafter, the rhythm was returned to normal sinus rhythm with bradycardia.