자동생화학 분석기 Hitachi 7180의 평가

신경섭,주대일,김영숙 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

연구목적: 저자들은 본원에 응급화학검사기기로 새로 도입된 Hitachi 7180 (Hitachi, Tokyo, Japan)의 분석 능을 보기 위하여, 정밀도, 직선성, 정확성을 평가하였으며 기존의 화학검사기기인 Hitachi 747 (Hitachi, Tokyo, Japan)과의 상관성도 평가하였다. 대상 및 방법: 포도당, 크레아티닌, blood urea nitrogen (이하 BUN), 총단백, 알부민, aspartic transaminase (이하 AST), 나트륨, 칼륨, 클로라이드 등 총 9종목에 대하여 정밀도를 측정하였으며, 포도당, 크레아티닌, BUN, AST에 대하여 직선성 및 회수율을 구하였다. 포도당, 크레아티닌, AST 및 총 빌리루빈에 대하여 Hitachi 747과 상관관계를 평가하였다 결과: 일내 정밀도는 저농도 및 고농도 정도관리 물질에서 3.05% 및 2.02% 이하의 변이계수를 보였으며, 총 정밀도는 각각 4.4%와 2.42% 이하의 변이계수를 보였다. 직선성을 평가한 모든 항목의 결정계수(r²)는 0.999 이상이었다. 회수율은 평가한 4종목에서 100.0에서 103.08% 사이이었다. Hitachi 747과 상관검사에서 측정한 4종목의 상관계수(r)는 모두 0.9986 이상이었다. 결론: Hitachi 7180은 정밀도, 직선성 및 회수율이 우수하였으며, 기존의 기기와 좋은 상관성을 보여 임상검사실에서의 적용에 유용할 것으로 사료된다. Purpose: The authors evaluated a recently introduced chemical analyzer, Hitachi 7180 (Hitachi, Tokyo, Japan) for precision, linearity, accuracy and comparability with Hitachi 747 (Hitachi, Tokyo, Japan) to see if it was suitable for the stat analyzer. Material and Methods: The precision, linearity, accuracy and comparison study were performed. For twenty day, the precision was performed for glucose, creatinine, BUN, protein, albumin, AST, sodium, potassium and chloride. The linearity and recovery rates were performed for glucose, creatinine, AST, BUN. The comparison study was performed with Hitachi 747 for glucose, creatinine, AST and total bilirubin. Results: The within-run coefficients of variations (CVs) at low and high concentrations were less than 3.05% and 2.02%, respectively. The total CVs for each groups were less than 4.4% and 2.42%, respectively. The linearity was well maintained in range of medically significant levels(r²≥0.999). The recovery rates were good for all tested items (100-103.8%). In comparison study, coefficients of correlation exceeded 0.9986 for all tests. Conclusion: The precision, linearity and accuracy of Hitachi 7180 were good. The correlation with Hitachi 747 was good. We concluded that its analyzer can be useful for clinical laboratory.

인공모래 부산물 재활용 방안

김경남,신대용,이현종 강원대학교 부설 석재복합신소재 제품연구센터 2002 석재연 논문집 Vol.7 No.-

인공모래 제조시 부산물로 발생하는 석분슬러지에 의한 환경문제와 폐기되는 자원의 활용문제를 해결하기 위하여 석분슬러지와 점토 및 폐유리분말을 이용하여 900∼1,100℃로 소성하여 세라믹스 건자재를 제조하였다. 석분 슬러지는 평균입경 16.3㎛, 비표면적 0.42㎡/g, 화학조성은 CaO 46.43wt%, SiO_2, MgO 와 Al_2O_3가 7.83 wt% , 6.17 wt% 및 3.13 wt%이며, 주결정상은 calcite (CaCO_3)와 dolomite (CaMg(CO_3)_2)로서 약 800℃에서 CaCO_3나 CaMg(CO_3)_2의 열분해에 의한 32.52wt% 의 강열감량을 나타내었다. 석분슬러지와 점토를 이용한 시편(No. 1, 4, 7, 10 및 13)은 점토의 첨가량과 소성온도가 증가함에 따라 시편의 부피비중 및 압축강도는 증가하였으나 기공률과 흡수율은 감소하여 No. 1, 4, 및 7 시편은 부피비중 1.78∼1.98, 기공률 6.4∼12.5%, 흡수율 10.3∼12.7%, 압축강도 264∼370kgf/㎠을 나타내었다. 폐유리분말을 첨가한 시편(No. 2, 5, 6, 8, 9, 10 및 12)의 부피비중과 압축강도는 1.91∼2.63 및 213∼895kgf/㎠로서 폐유리분말의 첨가가 시편의 소결성과 압축강도의 향상에 기여하여 세라믹스 건자재로 사용이 가능하였다. The utilization of waste stone powder sludge produced secondarily from artificial sand plants of Ssangyoung resource development (Co.) and general waste, as was classified, became very important to solve the environmental problem and waste materials recycle. In the chemical composition of stone powder sludge, the amount of CaO was 46.43wt%, SiO_2, MgO and Al_2O_3 were 7.83 wt% 6.17 wt% and 3.13 wt%, respectively. The mean average particle size was 16.3㎛ and specific surface area was 0.42㎡/g. Main crystal phases were calcite (CaCO_3) and dolomite (CaMg(CO_3)_2) and ignition loss was 32.52wt% because of decomposition of CaCO_3 or CaMg(CO_3)_2. Specimens were fabricated by mixing the waste stone powder sludge, low-grade clay, waste glass powder and sintering at 900∼1,100℃ for 2 hours. The density and compressive strength of specimen increased with increasing the sintering temperature and the amount of clay. The specimen showed the bulk density of 1.78∼1.98, porosity of 6.4∼12.5%, water absorption 10.3∼12.7% and compressive strength of 264∼370 kgf/㎠. Those of the specimen heated at 1,050℃ increased with increasing the added amount of waste glass powder, bulk density was 1.91∼2.63 and compressive strength was 213∼895 kgf/㎠. Therefore, the waste stone powder sludge can be used as a constructional materials.

목초액의 엽면살포가 선학초(Agrimonia pilosa L.)의 종자수량에 미치는 영향

정대수,신상현,김현경,오주성,이용호 東亞大學校 大學院 2002 大學院論文集 Vol.27 No.-

Agrimonia pilosa L. has been used as a medicinal plant in traditional folk remedy, and it shows inereasing tendency at various sections such as medicine-making material, functional food, and agricultural chemicals using plant or extract. The treatment was 400times solution(50㎖), 600times solution(33.3㎖), 800times solution(25㎖), 1,000times solution(20㎖) and 1,200times solution(16.7㎖) level with foliar application of wood extraction in Agrimonia pilosa L. The results obtained through these experiments were summarized as follows; plant height, stem number, leaves was increased with 800times solution(25㎖) foliar application of wood extraction. Among foliar application of wood vinergar soution diluted by 800-1,200 times was most effective considering growth and yield as compared other dilutions and control plot.

木村腐朽菌의 菌絲生長抑制에 대한 木村 防腐劑의 效果

민경희,정대교 建國大學校 附設 農業資源開發硏究所 1993 農資源開發論集 Vol.18 No.-

한천 평판 희석법에 의한 각 방부제의 목재부후균 T. palustris와 C. versicolor에 대한 생육저해 효과는 유용성 방부제인 IF-1000과 IPBC가 효과가 가장 크게 나타났으며, 수용성방부제 중에서는 CCB가 우수하게 나타났다. 또한 T. palustris보다 C. versicolor에 대해 방부제의 생육저해 효과가 크게 나타났다. IF-1000, IPBC, ZKCF, CCA, and CCB were tested for their ability to inhibit the mycelial growth of Tyromyces palustris and Coriolus versicolor. The inhibitory effect of chemicals was measured by the length of mycelial growth. IF-1000 and IPBC showed the highest inhibitory effect for Tyromyces palustris and Corigolus versicolor, And C. versicolor was more sensitive than T. palustris in chemical treatments.

Cortisone acetate 投與에 依한 Pneumocystis carinii 肺炎의 發症 課程

閔庚璇,李英河,羅榮彦,辛大煥 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

This study was undertaken to determine the provocative characteristics of experimental Pneumocystis carinii pneumonia in cortisone acetate treated rats. A Total of 55 male Sprague-Dawley rats, body weights 166-224g, were used. Five out of them were used as control group and remaining 50 were as experimental groups. In experimental groups, the rats were injected with 25mg of cortisone acetate twice a week for 10 weeks subcutaneously and drinking water contained 1000mg tetracycline hydrochloride per liter. Control group was not administered any drug. The 5 rats were sacrified weekly at each week in experimental group. but control group was sacrified at 10 weeks. For arterial blood gas analysis, blood was collected from carotid artery of rats using catheter. The rat's lung were removed after killed. The stamp smeared specimens of lungs were prepared and stained toluldin blue 0 for the quantification of P.carinii cysts. For the histopathologic observations, lungs were fixed in 10% buffered neutral formalin, cut into sections and stained with hematoxylin-eosin stain. For the histologic score of the intensity of P.carinii pneumonia, the sections were stained with Gomori's methenamine silver stain. The results obtained were as follows : 1. In the arterial blood gas analysis of cortisone acetate treated rats, blood pH and PaCO_2 was showed normal range. But PaO_2 was slightly decreased in experimental period. 2. Histopathologic finding of H-E staining sections were found in order of infiltration of lymphocyte and alveolar macrophage, eosinophilic foamy exudate, thickening of alveolar septum, desquamation of pneumocyte and fibrinoid degeneration and so on, but plasma cell was not found. 3. The histologic score of the intensity of P.carinii pneumonia was increased in proportion to the cortisonized periods of rats. 4. The number of cysts in the stamp smeared specimens of lungs were increased in proportion to the cortisonized periods of rats. And highly significant correlation was found between the histologic score of the intensity of P.carinii pneumonia and the number of cysts countered in the stamp smeared specimens of lungs(r=0.8620, ANOVA test p<0.0001). The present study revealed that provocative characteristics of experimental P. carinii pneumonia was highly significant relationship with the histologic score of P. carinii pneumonia and the number of cysts in the stamp smeared specimens of lung.

工團 周邊 農耕地 土壤의 成分 變化에 관한 硏究

鄭大守,金度勳,金賢勍 東亞大學校 1995 東亞論叢 Vol.32 No.-

溫山工業團地 造成에 따른 土壤汚染의 정도를 調査하기 위하여 1988年부터 1994年까지 7년간에 걸쳐 工團 隣近地域의 논과 밭 土壤에 대한 土壤成分의 理化學的인 變化를 調査分析한 結果를 要約하면 다음과 같다. 土壤의 pH 變化는 논과 밭 土壤 모두 每年 pH가 약간씩 減少하여 土壤 酸性化가 深化되어지고 있으며, 一部 地域에서는 pH 5.0 以下의 强酸性을 나타내는 곳도 있었다. 논 土壤의 特性變化에서는 有機物含量과 有效燐酸의 含量은 全國平均보다 낮았으며, Ca++이온과 Mg++이온의 含量은 每年 增加하는 傾向이었다. 밭 土壤의 特性變化에서도 有機物含量과 有效燐酸의 含量은 全國平均보다 낮게 나타났으며, K이온은 全體 平均이 0.85me/100g으로 全國平均 0.5m3/100g보다도 높은 含量을 보였고, Ca++이온과 Mg++이온의 含量은 每年 增加하는 傾向을 나타내었다. A study was carried out to investigate the possibility of soil pollution in Onsan Industrial Complex. Physical and chemical properties of soil were analyzed. Soil samples were collected from paddy and upland soil around the Onsan Industrial Complex between 1988 and 1994. The results were summarized up as follows: Soil acidity of paddy and upland soil was slightly decreased every year respectively. Some sampling area and showed the strong acidity which was less than pH 5.0. For the characteristics of paddy soil, the content organic matter and phosphroic acid were lower than that of Korean average. The content of Mg ++ and Ca++ were increased by degree every year. In the case of upland, the content of organic matter and phosphoric acid showed similar pattern as low as Korean average. The content of K+ was higher as the value of 0.85me/100g comparing with the Korean average, 0.5me/100g. For the Mg++ and Ca++, the contents were also increased by degree every year, respectively.

페나진 유도체들의 抗菌性에 관한 분자궤도론적 연구

郭庚度,朴柄珏,金鎬順,徐萬哲,金鐘大 嶺南大學校附設 基礎科學硏究所 1988 基礎科學硏究 Vol.8 No.-

The electronic states of the antimicrobial phenzine and its derivatives were examined by Extended H?uckel Method. It was found that the sum of nucleophilic reactivity index for trans 1,2, and 4 positions, ?? in a molecule was found to be related antimicrobial activity. The index, ??, should be applied to biological activity as well as antimicrobial activity.

Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

Karst 地形에 관한 硏究 : 江原道地域을 中心으로 Focused on Kangwon-Do Regions

金大經 건국대학교 1978 論文集 Vol.8 No.1

Limestones forming Karst togography in the Province of Kangwon-do are found growing better when the amount of Ca 0 contents proves to be above 50 per cent, and corrosion is found active when joints and fissures are steep. In the polje of Ssang Yong-ri, Yeong Weol-kun lies a layer of peat-bed formed bur the flood in the postglacial period, and in the polje of Chuam-ri, Samchop-kun also lies a layer of peat-bed formed by a sea-transgression. The beginning period of Karst topography is estimated to be two million years ago in view of the fact that terra rossa is less than five meters thick. On the Karst of Yeongdang-ri, Yeongweol-kun and Seongnam-ri, Samchop-kun still lie pebbles resulted from the great flood in the postglacial period. In the terra rossa of Kumi-ri, Samchop-kun and Pohang there lie gravel beds which are the proof of upheavals. The lower Karst developing along the eastern coast is found to have been the Karst belonging to the Continental shelf before the upheaval. The polje in Ahmok-ri, Siseon-kun is most likely to belong to glacial pljes made up of piedmont glacier curing the period of Wu¨rm glacier. It is found that the Karst topography of Kangwondo-province is a composite topography resulted from the working actions of corrosion, glacial, and denudation.

민어(Miichthys miiuy)의 卵發生過程 및 仔稚魚 발육단계에 따른 형태발달

한경호,오성현,황동식,조영현,서대철 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.2

본 연구는 2000년 8월 19일 전라남도 신안군 임자도 연안에서 2각망으로 어획된 민어 어미를 인위적인 방법으로 수정하여 부화 사육하면서 관찰한 卵發生 過程 및 仔稚魚 형태변화는 다음과 같다. 1. 민어의 受精卵은 구형의 분리 부성난으로 알의 크기는 0.96~1.08㎜(평균 1.01㎜, n=50)였고, 크고 작은 油球가 1~5개(0.06~0.34㎜) 존재하였다. 2. 사육수온 25.2~28.4℃에서 受精 후 17시간 10분에는 배체가 卵膜을 뚫고 머리부터 孵化하기 시작하였다. 3. 孵化 직후 仔魚의 전장은 2.36~2.63㎜(평균 2.48㎜, n=10)로 전장의 45.0~47.0%에 달하는 큰 卵黃(장경 1.11~1.20㎜, 단경 0.92~0.96㎜)을 가지고 있으며 筋節은 7~8+ 17~19=24~27개였다. 4. 孵化 후 3일째 仔魚는 전장 3.98~4.32㎜(평균 4.16㎜, n=10)로 입은 열려있었고, 이 시기에 섭이활동이 활발하게 관찰되면서 卵黃이 완전히 흡수되어 後期仔魚期로 이행되었다. 5. 孵化 후 10일째 後期仔魚는 전장이 5.88~6.74㎜(평균 6.30㎜, n=10)로 꼬리의 말단이 굽어지기 시작하는 시기로 黑色素胞가 기저후두골 뒷부분의 체측에서 점모양 및 나뭇가지모양 으로 출현하였다. 6. 孵化 후 31일째 개체의 전장은 9.62~12.76㎜(평균 11.12㎜, n=10)로 모든 지느러미가 정수에 달하였고, 체형이나 반문이 成魚와 닮아 種魚期로 이행하였다. Egg development and morphological change of larvae of the brown croaker, Miichthys miiuy, were investigated after obtaining samples by artificial insemination. The spawning season extends from the middle to the end of August in the seas off Lim-ja Island, Cheollanam-do. The fertilized eggs were transparent, spherical, separate and they floated. Egg diameter ranged 0.96~1.08㎜ with several oil globules of 0.06~0.34㎜. Hatching occurred 17 hours 10 minutes after fertilization in 25.2~28.4℃ water temperature. The newly-hatched larvae were 2.36~2.63㎜(mean:2.48㎜, n=10) in total length(TL), had a large yolk, and 7~8+17~19=24~27 myotomes. Melanophores were distributed on the eye lens. Three days after hatching the larvae had attained 3.98~4.32㎜ in TL(mean:4.16㎜, n=10), the yolk sac was completely absorbed, and they had transformed into the postlarva stage. The larva reached the juvenile stage with all fins formed and with a complete set of fin rays(D.Ⅹ-28~31;A.Ⅱ-7~8;P. 21~23;C. 19~21;V. 25~26) on the 31st day after hatching. The juveniles were 9.62~12.76㎜(mean:11.12㎜, n=10) in total length. At this point the juvenile was similar to the adult in body form and pigmentation pattern.