장구균의 동정에 대한 PCR 방법의 유용성

최연화,이영선,김홍빈,김치경,김봉수 대한감염학회 2001 감염 Vol.33 No.2

Background : Enterococci are important cause of nosocomial infections. Recently, vancomycin-resistant enterococci (VRE) has been increasingly reported as significant nosocomial pathogens. Therefore, accurate identification of enterococcal species is a prerequisite step for the appropriate antibiotic treatment and epidemiologic surveillance. We wanted to know the usefulness of PCR method compared with Vitek automatic identification system. Methods : Totally 105 isolates were identified on the species level by Vitek (GPI card and software version R06.1), methyl-α -D-glucopyranoside test, and PCR methods. Results : Among 105 enterococcal isolates, 59 were identified as E. faecium, 11 E. faecalis, 6 E. gallinarum by Vitek. But 29 isolates (28%) were unidentified. Subsequently all of these isolates were analyzed by PCR, the results of which were as follows : 17 E. faecium, 5 E. casseliflavus, 7 E. gallinarum. Two isolates identified as E. gallinarum by Vitek were reidentified as E. casseliflavus by PCR and other methods far phenotypic characterization. Conclusoin : PCR method was more accurate and sensitive than Vitek for the identification of enterococci species. (Korean J Infect Dis 33:123∼127, 2001)

하이퍼텍스트 학습환경에서 학습전략, 메타인지, 작동기억이 성취도에 미치는 영향

오선아,진화봉 한국교육정보미디어학회 2004 교육정보미디어연구 Vol.10 No.1

본 연구의 목적은 하이퍼텍스트 학습환경에서 학습전략과 메타인지, 작동기억이 청취도에 어떤 영향을 주는지, 그리고 이들 중 어떤 변인이 성취도에 가장 많은 영향을 주는지 검토하는 것이었다. 이를 위해 초등학교 6학년 92명을 텍스트 전략집단, 하이퍼텍스트 전략집단 등 두 개의 실험집단과 각각의 비교집단을 구성하여 실험하였다. 실험집단에는 피험자의 인지부하를 줄이기 위하여 텍스트를 핵심내용으로 제시하는 학습전략을 사용하고, 비교집단에는 학습전략을 사용하지 않았다. 실험결과, 텍스트 전략집단과 하이퍼텍스트 전략집단의 성취도는 예상했던 대로 각각 비교집단보다 유의하게 높은 것으로 나타났다. 메타인지 수준에 따른 성취도는 메타인지적 규제 상위집단의 성취도가 하위집단보다 높았으나, 메타인지적 지식 수준에 따른 성취도 차이는 발견되지 않았다. 작동기억 수준에 따른 성취도는 중앙집행장치 용량 상위집단이 하위집단보다 높았으나, 언어적 작동기억, 시·공간적 작동기억 용량 상위집단의 성취도는 기대와는 달리 하위집단과 유의미한 차이가 나타나지 않았다. 학습전략, 메타인지, 작동기억 중에서는 메타인지적 규제와 작동기억의 중앙집행장치가 하이퍼텍스트 학습 성취도를 가장 잘 설명하는 변인의로 밝혀졌다. 이 연구결과는 하이퍼텍스트 학습 환경은 메타인지와 작동기억을 고려해서 외적 인지부하를 줄여 정합성을 높여야 하며, 적절한 스키마 형성 인지부하를 증가시켜 주는 교수설계가 필요하다는 것을 시사한다. The purpose of this study was to examine the effects of learning strategies, mtaccgnitim, warkng rzlemcny on achievement in the hypertext leaming enviromt and also to review what the m t influential variables is in the hyptext learning. The subjects were 92 sixth @;I-c.rders. They were assigned to either experimtntal groups (text-strategy group, hypertext-strategy group) and control groups (text-strategy control group, hypertext-strategy control group). The expehmtal groups performed a learning strategy to recluce cognitive load In contrast, the control groups did not make use of a learning strategy. The results were as follows: As expected, the experimental groups significantly outformed the control groups. The high metacognitive regulation group achieved hgk scores than its low counteppart. However, there appeared to be no significant diffemms on the level of mtaccgnitive knowledge. As the level of workmg memory span, the central executive span affected achievemnt. However, the phonological loop and visuo-spatial working mmemory span didn't significantly affect achievement. The results of study demostrate that metacognitive regulation and centml executive are influential variables that can be used to help achievement in hyperkxt The finrhngs suggest that metacognition and workmg m e memory should be taken into consideration in hypatext instrudional design in order to reduce exhnmus cognitive load and haease germane cognitive load

흰쥐중심편도체핵에서 Neurotensin 면역양성세포의 다리옆핵으로의 투사

박인식,최정화,박봉수,백선용,김봉선,김재봉,김진정 대한해부학회 1996 Anatomy & Cell Biology Vol.29 No.2

무도 종목별 스포츠 적성 체력 판별에 영향을 미치는 체력 요인 분석

최종삼,윤익선,봉화선 龍仁大學校 武道硏究所 2004 武道硏究所誌 Vol.15 No.1

The standard of value of winning in sports has become much important and the standard of physical strength improvement serves as the most important conformity for assessment of players, thus, it is required to identify what are the major factors for performance of play to improve the technique of Yongmoodo, Taekwondo and Kumdo athletes. Accordingly, researchers, scientists, and leaders of sports analyse the factors for performance of play focussed on how to improve the performance of play, and anticipate to analyse, to be informed of, and to assess the players and training result based on the result of play. Thus, the study is designed to find out the physical factors for decision of records of play in university students Yongmoodo, Taekwondo and Kumdo athletes under planned training and to identify physical factors of play performance of Yongmoodo, Taekwondo and Kumdo athletes. SPSS V11.0 ststistic program was used in processing of data in this study. Stepwise discriminant analysis was applied in identification of major assessment items showing the difference between the groups, and partial F-test statistic was utilized, on the assumption that contribution ratios of each variable are the difference between the groups, to assess their statistical significance and to make decision whether to select the variables. 1. In determination of Yongmoodo, Taekwondo and Kumdo athletes by physical strength, statistic showed statistical significance with 5% of significance level. 2. The order of contribution of variables that determine the performance of play as well as run to 100m, back strength, abdominal muscular endurance, flexibility, muscular endurance of upper arms are Yongmoodo athletes, thus, grip strength, agility and 1 mile run are Taekwondo and Kumdo athletes. 3. In therms of accuracy of the reclassification of determination factors as sports talent fitness of martial-arts event style, the reclassification into Yongmoodo(78.8%) and Taekwondo(86.8%) was higher compare that of Kumdo(37.7%). In therms of accuracy of the reclassification of determination factors as sports talent fitness of all martial-arts event style, the reclassification into Yongmoodo, Taekwondo and Kumdo were 69.8%.

조사연구에서 표본설계에 대한 고찰

양철호,양선화,신봉관,조지현 동신대학교 2005 論文集 Vol.15 No.-

Most of the survey research are conducted with samples. Thus, representativeness of the samples is crucial for the sound research. Reports of the various survey research were analized. The results of the analysis showed that different ways of sampling methods were utilized in various studies in order to ensure the selection of unbiased samples under the circumstances of purpose and population of the survey.

GC-ECD를 이용한 유기염소계 및 Pyrethroid계 농약 동시 분석법

김우성,이선화,김재이,정지윤,이명자,박영채,이영자,정성욱,이봉헌,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with florisil, and finally analyzed simultaneously the organochlorine and pyrethroid pesticides using GC(ECD). An ultra-2 fused silica capillary column was used to separate and identify the products. The resolution between the last isomeric peak of cypermethrin(59.987min) and the first isomeric peak of flucythrinate(60.043min) was not satisfactory. The last isomeric peak of fenvalerate(62.344min) and the first isomeric peak of fluvalinate(62.397min) were overlapped. Recoveries of soybean sample for the most pesticides were 73.3% to 102.4%. Detection limits were between 0.004 and 0.063 ㎍/㎎ when this method was used.

Simultaneous determination of 19 new psycoactive substances in human plasma for quantitative analysis with LC-MS/MS

Sun-hwa Bong,Hyewon Seo,Hye Jin Cha,Jisoon Shin,Young-Hoon Kim,Hye-Kyung Park,Hyung Soo Kim,Jin Hong 한국분석과학회 2017 학술대회논문집 Vol.2017 No.11

Nuclear Argonaute 2 regulates adipose tissue‐derived stem cell survival through direct control of miR10b and selenoprotein N1 expression

Kim, Bong Sun,Jung, Jin Sun,Jang, Jin Hwa,Kang, Kyung Sun,Kang, Soo Kyung Blackwell Publishing Ltd 2011 Aging cell Vol.10 No.2

<P><B>Summary</B></P><P>Argonaute 2 (Ago2) has a leading function in miRNA‐induced RNA silencing, a conserved gene regulatory mechanism in cells and organisms. miRNAs are critical for stem cell self‐renewal, development, and other functions. Here, we report that nuclear Ago2, by binding to a specific region of functional genes, directly controls adipose tissue–derived stem cell (ATSC) survival in response to a critical dose of reactive oxygen species (ROS)‐mediated oxidative cell damage or senescence. The role of nuclear Ago2 has not been previously reported. Here, we show that human ATSCs in which Ago2 was downregulated underwent apoptosis. Silencing of Ago2 in ATSCs significantly induces upregulation of miR10b and miR23b expression. These miRNAs directly interfere with ROS‐scavenging gene expression, such as TXNL1 and GPX3. Upregulation of miR10b and miR23b is sufficient to induce ATSC cell apoptosis via p38 MAPK phosphorylation and caspase 3 activation. In addition, Ago2 overexpression or interference by miR10b and miR23b expression in ATSCs partially rescued H<SUB>2</SUB>O<SUB>2</SUB>/ROS‐mediated apoptotic cell death by upregulating the expression of TXNL2, JUNK, caspase‐3, and cytochrome C. Nuclear Ago2‐mediated miR10b and miR23b downregulation also allows cells to escape senescence, which results in telomerase reverse transcriptase, stemness overexpression, and improved self‐renewal and differentiation through Wnt5a/β‐catenin activation. Argonaute 2 expression is critical for stem cells to escape senescence by downregulating miR10b and miR23b. The Ago2‐binding gene selenoprotein N1 (SEPN1) was also effectively involved in ATSC survival and self‐renewal through ROS‐mediated p38 MAPK inactivation.</P>

광 촉매 이산화 티타늄의 개 피부에 대한 항균효과

장화석,김지은,정다정,이정선,최치봉,김휘율,Chang, Hwa-Seok,Kim, Ji-Eun,Chung, Dai-Jung,Lee, Jung-Sun,Choi, Chi-Bong,Kim, Hwi-Yool 대한수의학회 2006 大韓獸醫學會誌 Vol.46 No.3

Photo-catalytic products have been widely used at home and hospital to prevent bacteria, virus and fungus. Activities of anti-bacteria, anti-viruses and anti-fungi are based upon direct contact of crystals and particles of titanium dioxide with pathogens, into which titanium is catalyzed by photo. Those antimicrobial activities of the photo-catalytic titanium dioxide have been proved in vitro. However, in vivo tests of those activities have not been carried out on dog skin. Aim of this study was to evaluate the antimicrobial activities of the catalytic titanium dioxide in vivo. Ten beagle dogs were divided into two groups. One group was sprayed with 10ml of titanium dioxide(1 mg/ml) whereas the other was not. The treated dogs were exposed under the sunlight for 120 min. A set of three hairs was taken 15, 30, 60 and 120 min after the exposure and the bacteria contaminated in hairs were amplified in, Muller Hilton broth at $35^{\circ}C{\pm}1$ for 3 h. The supernatant of the bacterial culture was diluted 1 : 10 in phosphafe-buffered saline. One milliliter of the diluents was transferred into triphenyltetrazolium medium(TTC) and incubated at $35^{\circ}C{\pm}1$ for 2 days. The number of bacteria was counted. The number of bacteria colonies was decreased compared to control group. To further investigate the longevity effect of titanium dioxide, the dogs were kept in indoor without sun light for 6 and 12 h, 1, 2, 3, 7, 14 days after exposure of the chemical during each 15, 30, 60 min. The number of bacteria colony in 1ml was counted. The number of bacterial colonies was decreased. Treated group is exposured by sun light during 15 min, the longevity effect of titanium dioxide is continued by 1 week. Treated group is exposured by sun light during 30, 60 min, the longevity effect of titanium dioxide is continued over 2 weeks. These data indicated that the photo-catalytic titanium dioxide may be used for prevent bacteria on dog skin.

Preparation and Characterization of Genetically Engineered Mesenchymal Stem Cell Aggregates for Regenerative Medicine

( Sun Hwa Kim ),( Hyung Ho Moon ),( Bong Genn Chung ),( Dong Hoon Choi ) 한국약제학회 2010 Journal of Pharmaceutical Investigation Vol.40 No.6

Combining cell- and gene-based therapy is a promising therapeutic strategy in regenerative medicine. The aim of this study was to develop genetically modified mesenchymal stem cell (MSC) aggregates using a poly(ethylene glycol) (PEG) hydrogel micro-well array technique. Stable PEG hydrogel micro-well arrays with diameters of 200 to 500 μm were fabricated and used to generate genetically engineered MSC aggregates. Rat bone marrow-derived MSCs were transfected with a green fluorescent protein (GFP) plasmid as a reporter gene, and aggregated by culturing in the PEG hydrogel micro-well arrays. The resultant cell aggregates had a mean diameter of less than 200 μm, and maintained the mesenchymal phenotype even after genetic modification and cell aggregation. Transplantation of MSC aggregates that are genetically modified to express therapeutic or cell-survival genes may be a potential therapeutic approach for regenerative medicine.