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흰쥐의 장관내 점액질 성상에 미치는 Bassa 살충제의 영향에 관한 조직화학적 연구
김진정,조운복 부산대학교 1981 자연과학논문집 Vol.31 No.-
This experiment was performed to observe the effect of carbamate pesticide(Bassa) on the production, secretion, and properties of the mucosubstances in the duodenal glands, and the goblet cells of the duodenum, ileum, proximal colon, distal colon and rectum with histochemical methods. Healthy adult rats weighing about 250~300g each were divided into two groups-normal and experimental. Experimental groups each were administered Bassa, and sacrificed in 1,3,6,12,24,48, and 72 hours after administration of the pesticide. The mucosubstances were stained with alcian blue pH 2.5, alcian blue pH 1.0, alcian blue pH 2.5 - periodic acid Schiff(PAS), alcian blue pH 1.0 - PAS or aldehyde fuchsin pH 1.7 - alcian blue pH 2.5. The obtained results were as follows: 1. In the small intestine, the amount of neutral mucins in the duodenal glands were most remarkable in 6 and 12 hours with Bassa. Amounts of neutral mucins in diminution were gradually recovered from 48 hours after administration. The diminution of mucosubstances in the goblet cells of the small intestine after administration was more noted in upper villi and lower crypts, and decrease of acid mucins was more notable than that of nuetral mucin. That is, the diminutions of acid mucins were most remarkable in 1 and 3 hours (ileum), and 1 to 12 hours (duodenum) after administration. The diminished acid mucins in duodenum and ileum started to recover from 48 hours after administration. The diminution of neutral mucins were most remarkable in 6 and 12 hours (duodenum), and were marked in 1 to 6 hours (ileum) after administration. The gradual recovery of nuetral mucins in 24 hours (duodenum) and 12 or 24 hours (ileum) after administration was noted. In villi and crypts of the small intestine after Bassa administration, goblet cells secreting strong sulfated mucin and goblet cells secreting nonsulfated mucin both were severely decreased staining properties, and the formers were more severely effected than the latters. The recovery of the former goblet cells was more delayed than the latters. And this pesticide was more severely effected to the duodenum than ileum. 2. In the large intestine, the amount of mucosubstances in the goblet cells after administration was more noted in surfaces and lower crypts, and decrease of acid mucins was more notable than that of neutral mucin, that is the diminution of acid mucins were most remarkable in 1 hour (rectum), 1 and 3 hours (distal colon), and 1 to 6 hours (proximal colon) after administration. The recovery of acid mucins in 12 hours (distal colon and rectum), and in 48 hours (proximal colon) after administration was noted. The diminution of neutral mucins were most remarkable in 1 hour (distal colon) and 1 to 6 hours (proximal colon), and were marked in 1 to 6 hours (rectum) after administration. Amount of neutral mucins in diminution were gradually recovered from 12 hours (distal colon and rectum) and 24 hours (proximal colon) after administration. In surfaces and crypts of the large intestine after Bassa administration, goblet cells secreting strong sulfated mucin and goblet cells secreting nonsulfated mucin both were decreased staining properties, and the formers was more remarkable than the latters, furthermore this pesticide was more severely effected to the proximal colon than distal colon and rectum. On the basis of the above results, it is suggested that were severely inhibited to production and secretion of acid and neutral in earlier time period of the experiment, and the production of acid mucins were severely inhibited more than neutral mucin. In comparison with the small and large intestine, the small intestine seems to be inhibited more than the large intestine. In comparison with organs, the duodenum seems to be inhibited more than ileum in small intestine, and the proximal colon more than distal colon and rectum in large intestine.
金鎭晶,池善奎 최신의학사 1968 最新醫學 Vol.11 No.11
Author observed two cases of atlanto-occipital assimilations in adult skulls in Korean. In the first case, the atls and the occipital bone was completely fused, being separated only between occipital bone and left transverse process of the atlas and occipital bone had depression upon the pars basilaris. Thus it could be said type Ⅱ after the classification of Blomquist and Ingelmark method. It was also fused between the axis and the third cervical vertebra In the second case, the atalnto occipital articulation was completely fused and the right part of the arcus anterior and posterior of the atlas was also completely fused with the corresponding margin of the foramen magnum, but the depression upon the pars basilaris of the occipital -bone could not, be seen. Thus it could be said type 1f after the classification of Blomquist and Ingeluiark method. , According to Bystrow's method the two cases described above_it.could be said the congenital type of the atlanto-occipital assimilation.