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개의 Hemoglobin, Albumin 및 Transferrin의 형(型)에 관(關)한 연구(硏究)
김우권,Kim, Woo Kwon 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.2
Hemoglobin, albumin and transferrin phenetypes observed in a population of 255 dogs of various breeds by agar gel electrophoresis for hemoglobin and albumin, and by starch gel electrophoresis, for transferrin. The results obtained were as follows: 1. The hemoglobin phenotypes were classified as HbAA, HbAB and HbBB and the frequencies of appearence were 91.77, 6.27 and 1.96%, respectively. Gene frequency studies of $Hb^A$ and $Hb^B$ showed the $Hb^A$ gene to have a frequency of 94.9% and $Hb^B$ gene to have a frequency of 5.1. The frequency of hemoglobin phenotypes of the Korean Jindo dog (ylelow) was HbAA 76.19%, HbAB 17.46% and HbBB 6.35% and HbBB was recognized only in the Korean Jindo dogs. 2. The albumin phenotypes were calssified as AlbAA, AlbAB and AlbBB and the frequencies of appearence were 28.71, 43.57 and 27.72% respectively. Gene frequency stduies of $Alb^A$ and $Alb^B$ showed the $Alb^A$ gene to hate a frequency of 50.50% and $Alb^B$ gene to have a frequency of 49.50. The frequency of albumin phenotypes of the Korean Jindo dog was 26.32% for AlbAA, 34.21% for AlbAB and 39.47% for AlbBB. The frequency of albumin phenotypes of the Korean Jindo dog was slightly different from others and gene frequency of $Alb^B$ was 56.58. 3. Transferrin studies resulted in findings of 10 different phenotypes and frequencies of transferrin phenotype were 30.08% for $TfA_1A_1$, 8.02 for $TfA_2A_2$, 2.11 for TfBB, 4.64 for $TfC_1C_1$, 1.69 for $TfA_1B$, 41.35 for $TfA_1C_1$, 5.49 for $TfA_2C_1$, 0.84 for $TfA_2C_2$ and 3.79 for $TfBC_1$. Gene frequency studies of $Tf^A$, $Tf^B$ and $Tf^C$ showed the gene to have a frequency of 63.08% for $Tf^A$, 4.85 for $Tf^B$ and 32.07 for $Tf^C$ 4. Transferrin studies revealed two different band patterns on starch gels with a strong and weaker stained one. The $TfC_2C_2$ and $TfA_2A_2$ which were found in the Korean Jindo dog only showed a weaker stained band and the gene frequency of $TfA_1A_1$ was highly recognized in this dog.
임신유우(姙娠乳牛)의 혈청중(血淸中) Vitamin E 농도(濃度)
용만중,Yong, Mahn Joong 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.2
Blood serum from 30 pregnant cows, 6 non-Pregnant cows (one month after delivery), 6 sterile cows (ovarian cyst), and 6 heifers (16 months old) were analyzed for total vitamin E concentration by the ferric chloride-bipyridyl reaction. The pregnant group was determined periodically throughout pregnancy. The results obtained were as follows: 1. The mean value of serum vitamin E level in pregnant group was $0.651{\pm}0.068(SE)mg/100ml$, in non-pregnant group $0.647{\pm}0.119(SE)mg/100ml$, in sterile group $0.488{\pm}0.053(SE)mg/100ml$, and in heifer group $0.083{\pm}0.016(SE)mg/100ml$. Samples below 0.19mg/100ml were net observed and most of the pregnants (83.3%) showed the values of 0.40 to 0.99mg/100ml during pregnancy 2. The difference of vitamin E level between pregnant group and non-pregnant group was net significant, but these trio groups showed a significantly higher value than that of sterile group. On the other hand, in heifer group was recognized significantly lower value compared with adult groups. 3. The change of serum vitamin E level in pregnant group revealed the tendency of decrease as gestation progresses and significantly dropped at the eighth month, thereafter, the value was rose slightly at the tenth month of gestation but no differences were observed. The coefficient of correlation between months and serum vitamin E levels during pregnancy was r=-0.956(p<0.01), and a regression equation was Y (serum vitamin E levels, mg/100ml)=1.034-0.063x(month).
몇가지 자율신경성약물(自律神經性藥物)이 Reserpine 처리(處理) 병아리의 척출공장(剔出空腸) 내재성신경(內在性神經)에 미치는 영향(影響)
이창업,Lee, Chang Eop 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.2
Pharmacological investigation was performed in chickens which were pretreated with reserpine. Transmural stimulations were given to the isolated jejunum of chickens and studied the responses and the effects of dibenamine, propranolol, atropine and tetrodotoxin on them. The results obtained were summerized as follows: 1. Three different patterns of response were obtained from the isolated jejunum of non-treated chickens after giving them transmural stimulation. The first pattern was contraction followed by relaxation, the second pattern was contraction only and the third pattern was relaxation only. 2. The transmural stimulation of the jejunum preparations evoked relaxation while the periarterial stimulation evoked contraction in the reserpinized chick. 3. The relaxation response to the transmural stimulation was not affected by the pretreatment with dibenamine, propranolol and atropine. 4. The relaxation response to the transmural stimulation was abolished by tetrodotoxin. The results obtained in these studies indicate that the relaxation response to the transmural stimulation is due to non-adrenergic inhibitory fibers.
세균성자궁질환(細菌性子宮疾患)에 있어서 Estrogen 이 미치는 영향(影響)에 관한 연구
오수각,옥종화,Oh, Soo Kak,Oak, Chong Wha 대한수의학회 1972 大韓獸醫學會誌 Vol.12 No.1
Estrous and non-estrous rabbits were inoculated with E. coli or Streptococcus pyogenes, and the mixture of the two organisms, and bacterial count and histopathological studies of uterine horns were made to observe the effects of estrogen on the resistance of the uterus to bacterial infection. The results obtained were summarized as followings; 1. Four hours after inoculation of bactoria into uterine horn, the number of organisms was significantly lower in estrous rabbits than in non-estrous regardless of the kind of organisms inoculated. 2. The highest reduction rate of the organisms among the three bacterial inoculation groups was found in estrous rabbits inoculated with E. coli, and the lowest reduction rate was with Streptococcus pyogenes. 3. Histopathological changes of uterine horns induced five days after bacterial inoculation were observed. In estrous rabbits, a mild inflammatory reaction was found in Streptococcus pyogenes group, but a slight inflammatory reaction and only a negligible inflammatory reaction were observed in mixed bacteria group, and in E. coli group respectively. In non-estrous group, however, a marked inflammatory reaction was observed in Streptococcus pyogenes group, a moderate inflammatory reaction and a slight inflammatory reaction were observed in the mixed bacterial group and E. coli group, respectively.
비환(鼻環)에 의한 돈폐충증(豚肺蟲症)의 예방(豫防)에 관한 연구(硏究)
임정택,윤화중,한방근,김영홍,Lim, C.T.,Yoon, H.J.,Han, B.K.,Kim, Y.H. 대한수의학회 1972 大韓獸醫學會誌 Vol.12 No.1
1. Of 4,112 earth worms, 3,480 were classified as Eisenia foetidae, and 3,277 of the species were infested with the larvae of Metastrongylus apri. 2. Cresol and $CaCN_2$ were found effective in the insecticidal test of earthworms. 3. The nose ring was effective, in pigs, to prevent digging and eating the earthworms and resulted a lower lungworm incidence in pigs. 4. The best place to put the nose ring was the nasal septum.
경주마(競走馬)와 제주마(濟州馬)의 생리적(生理的) 골경도(骨硬度)에 관하여
이원창,김효중,신태홍,Lee, Won Chang,Kim, Hyo Jung,Shin, Tae Hong 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.2
Although considerable research has been done on the blood chemistry of domestic animals, little work has been made of the changes associated with age. Moreover, the records about physiology of the goat were not much available in Korea, and a comprehensive survey of the blood chemical values of the Korean native goat has not been made. The authors intended to biometric study on the hardness of bone of race horse and Jeju horse in Korea. The measurement of hardness of bone were performed in 272 race horses (Thoroughbred 91, Anglo-Arab 107, Arab 74) and in 109 Jeju horses by the caliper by Toryba's method. Some interesting facts obtained through this study were as follows: 1. There was not significant difference of bone hardness between male and female. The average of bone hardness by the Toryba's meteod were $23.07{\pm}1.01$ in race horse and $19.44{\pm}1.84$ in Jeju horse. 2. The grade of bone hardness of race horse were higher than those of Jeju horse(P<0.001). 3. The correlation coefficient between age and grade of bone hardness were r= +0.344 in Jeju horse, theme were statistically significant (P<0.01) and the regression equation was Y=0.29x+18.497.
마우스의 실험적(實驗的) 고사리 중독(中毒)에 관한 병리학적(病理學的) 연구(硏究)
임병무,이호일,Rim, Byung Moo,Lee, Ho Il 대한수의학회 1972 大韓獸醫學會誌 Vol.12 No.2
In order to investigate the effects of bracken fern (Pteridium aquilinum) on animals, a total of 105 mice given a ration containing different percentage of bracken fern were studied pathologically during the experimental period of 90 days. The results obtained were as follow: 1. Mice in the group, given a ration containing 15% of bracken fern, showed 85% of death rate in 90 days, and mice in the group, given a ration containing 25% of it, showed 100% in 30 days 2. The experimental mice showed decreased myeloid cells and significant reduction in erythrocytes, leukocytes, and thrombocytes. 3. Severe congestion and hemorrhages were observed in lungs, liver, kidney, spleen, stomach, intestine, brain, adrenal and testis from the ones that died during the experimental period, but the necropsies of the latter period indicated mild chanties comparatively. 4. Mice in the group, given a ration containing 15% blacken fern showed a decreasing tendency in body weight.
김태종,이강욱,Kim, Tae Jong,Lee, Kang Wook 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.2
The blood picture of 85 healthy race horses in Korea was investigated. The ranges and mean values of erythrocyte, hemoglobin, hematocrit value, mean corpuscular volume, mean corpuscular hemoglobin concentration, and total white blood cell count in the blood picture were determine. The respective mean value and standard deviation and age differences were as follows: 1. The erythrobyte count was shown as range of 6.20 to $11.32{\times}10^6/mm^3$ with mean of $8.61{\pm}1.92{\times}10^6/mm^3$(SD). The leucocyte count was shown as range 5.0 to $18.0{\times}10^3/mm^3$ with mean of $8.25{\pm}1.51{\times}10^3/mm^3$(SD). There were not significant. differences in age, 2. The mean value of hemoglobin was shown $13.9{\pm}1.7g/100ml(SD)$ ranging 9.8 to 16.8g/100ml. The mean value of hematocrit was shown $40.9{\pm}3.94ml/100ml(SD)$ ranging 26 to 54. There were not significant differences in age. 3. The mean corpuscular hemoglobin was shown as range of 11.8 to 22.2pg with mean of $16.9{\pm}4.69$(SD). The mean corpuscular volume was shown as range of 34.5 to $71.3cu{\mu}$ with mean of $49.0{\pm}7.32cu{\mu}$(SD). The mean corpuscular hemoglobin concentration was shown as range of 30.6 to 39.4 g/100 ml with mean of $34.6{\pm}2.36$(SD). There were not significant differences in age. 4. The correlation among erythrocyte count, hemoglobin and hematocrit value were observed as follows: Erythrocyte count and hemoglobin (+0.328), rythrocyte count and hematocrit vague (+0.319). A linear regression equation was shown as follows: Erythrocyte count and hemoglobin (Y=0.336x+10.977), erythrocyte count and hematocrit value (Y=0.655x+35.274). 5. The high correlation between hemoglobin and hematocrit vague was observed (r= +0.836). A linear regression equation was shown: (Y=1.948x+13.895).
닭 부고환부(副睾丸部)의 각종(各種) 관상피(管上皮)의 형태(形態) 및 흡수(吸收)에 관한 연구(硏究)
이재홍,Lee, Jai Hong 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.2
Histological and histochemical studies were made on the lining epithelia of the various ducts in epidymis of the Rooster and absorptive function of the canal epithelial cells in the Rooster epididymis were also investigated after administration of India ink. The results obtained were summerized as follows; 1. Epihtelium lining the rate testis was mainaly composed of single later of cuboidal cells, and was partially composed of flattened squamous or low columnar cells. Efferential ductules were characterized by having many villous projections orrfolds which extened into the lumen, and were lined by stereociliated pseudostratified epithelium which consisted of manily ciliated columnar cells, a few scattered clear cells and basal cells. Connecting ductules were lined by ciliated pseudostriatified colummnar epithelium in which ciliated columnar cells, clear cells and basal cells were noted. Epididymal ducts were lined by pseudostratified epidhelium in which columnar and basal cells were noted. 2. PAS-granules, saliva resistant were noted mainly in the epithelial cells of efferential and connecting ductules. 3. Sudan black B stained heavily the granules in the epithelial cells of sufferential and connecting ductules. 4. The granules reactive to acid phosphatase most abundant in the epithelial cells of efferential ductules and were lesser amount in the epithelial cells of connecting ductules where as very few or no granules were seen in the rest of the ducts. 5. Alkaline phosphatase activity was most prominent but discontinuous in the luminal surface of the epithelium of efferential ductules and less marked in the connecting ductless. No enzyme activity was noted in the canal epithelium of epididyml duct. 6. India ink granules were most numerous in the epithlial cells of efferential ductules and were a few in connecting ductules. Very few or no granules of India ink were noted in the other types of the ducts. India ink granules in the epithelium increased gradually as the time after the administration of India ink (one up to twenty-nine hours) has proceeded. From those results it is suggested that epithelial cells of efferential and connecting ductules have active absorptive function, whereas the rest of duct system in the epididymis of the Rooster may be the mere pathway of the seminal fluid without significant modification of its constituents.