말벌 독성 물질과 그 유도체의 인지질 막 환경에서의 상호작용

이봉헌,박흥재 한국환경과학회 1998 한국환경과학회지 Vol.7 No.1

Toxic Mastoparan B(MP-B) which is purified from the venom of the hornet Vespa basalis is a cationic amphiphilic tetradecapeptide. MP-B and its Ala-substituted analogues were synthesized by solid phase method and the toxic peptide-membrane interactions were examined by circular dichroism(CD) spectra, fluorescence spectra, and leakage abilities in phospholipid membranes. In the presence of phospholipid vesicles, synthetic MP-B and its analogues formed amphiphilic α-helical structures, but in the buffer solution, those exhibited random coil conformation as measured by CD. Fluorescence spectra of MP-13 and its analogues which indicated the binding affinity of peptide on phospholipid vesicles showed that the replacement of Lys at position 2 and 11 with Ala caused a remarkable effect in the blue shift and that at position 2, in the leakage ability of the peptide.

Vibrio vulnificus , Escherichia coli 및 Salmonella typhimurium Lipopolysaccharide ( LPS ) 의 지방산 조성

이봉헌,박장수,강신원 ( Bong Hun Lee,Jang Su Park,Shin Won Kang ) 한국유화학회 1994 한국응용과학기술학회지 Vol.11 No.2

8-Azaxanthine과 그 유도체의 합성 및 시험관내 항암 활성

이봉헌,신정희,장태식,박장수,강신원,Lee, Bong Hun,Shin, Jung Hee,Jang, Tae Sik,Park, Jang Su,Kang, Shin Won 대한화학회 1997 대한화학회지 Vol.41 No.7

8-Azaxanthine(1), 3-${\beta}$-D-ribofuranosyl-8-azaxanthine(2), 3-${\beta}$-D-ribofuranosyl-8-azaxanthine-5'-monophosphate(3) 그리고 3-${\beta}$-D-ribofuranosyl-8-azaxanthine-5'-(3-pyridinylcarbonyl)monophosphate(4)를 합성한 후 Mouse leukemia P388, Murine mammary carcinoma FM3A, Human histiocytic lymphoma U937 암세포들에 대해 시험관내 항암활성을 MTT를 이용한 방법으로 예측하고 $IC_{50}$(${\mu}mol/mL$)으로 나타내었다. 그 결과, 비정상적인 염기의 N-3와 당의 C-1' glycoside 결합을 가진 2는 세가지의 암세포에 대해 감수성이 좋아 $IC_{50}$이 각각 0.05, 0.06, 0.06 ${\mu}mol/mL$이었으나 항세균 활성 예측에서는 감수성을 보이지 않았다. Human histiocytic lymphoma U937 세포에 대한 $IC_{50}$은 1이 0.33, 2는 0.66, 3은 0.25 그리고 4는 0.33 ${\mu}mol/mL$로 구조 변화에 따라 서로 다른 값을 보였다 8-Azaxanthine (1), 3-${\beta}$-D-ribofuranosyl-8-azaxanthine (2), 3-${\beta}$-D-ribofuranosyl-8-azaxanthine-5'-monophosphate (3), and 3-${\beta}$-D-ribofuranosyl-8-azaxanthine-5'-(3-pyridinylcarbonyl)monophosphate (4) were synthesized. The in vitro antitumor activities of the synthesized compounds against P388 mouse leukemia, FM3A mammary carcinoma, and U937 human histiocytic lymphoma cells were determined by MTT assay. 2 with unnatural N-3 and C-1' glycoside bond had activity against three tumor cell lines and $IC_{50}$s of these compounds were 0.05, 0.06, and 0.06 ${\mu}mol/mL$ against three tumor cell lines, respectively. But these compounds had no antibacterial activity. $IC_{50}$s against U937 human histiocytic lymphoma cells were verified with the structural modification: $IC_{50}$s of 1, 2, 3, and 4 were 0.33, 0.06, 0.25, and 0.33 ${\mu}mol/mL$, respectively.

환경 독성 Peptide의 인지질과의 상호 작용 특성 분석

이봉헌,박홍재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.1

The interaction of mastoparan B, a cationic tetradecapeptide amide from the hornet Vespa basalis, with phosphilipid bilayers was with synthetic mastoparan B and its analogue with instead of hydrophobic 12th amino acid residue in mastoparan B. MP-B and its derivative, [12-Ala]MP-B were synthesized by the solid-phase peptide synthesis method. MP-B and analogue, [12-Ala]MP-B adopted an unordered structure in buffer solution. In the presence of neutral and acidic liposomes, the peptides took an α-helical structure. The two peptides interacted with neutral and acidic lipid bilayers. These results indicated that the hydrophobic α-helix of MP-B critically affected biological and helical content.

연안 조류에서 Cd와 Zn 독성의 반대 작용 분석

이봉헌,김정호,정성욱,김성미,박흥재 한국환경과학회 2002 한국환경과학회지 Vol.11 No.10

The growth and heavy metal experiments revealed oppositive interactions between toxic metals(Zn and Cd) and Mn when the coastal diatom T. pseudonana were used. Cd and Zn inhibited the algal growth rate only at low Mn ion concentrations and this effect could be accounted for an inhibition of cellular Mn take by the toxic metals. Mn and Zn inhibited cellular Cd take and this indicated a reciprocal effects among the metals with respect to metal take. Saturation kinetics modeling of the take data was consistent with two metals competing with each other for binding to the Mn take system and with both Cd and Mn being transported into the cell by that system. Mathematical modeling of Mn and Cd take data revealed evidence for a Cd efflux system.

8-Azaxanthine과 그 유도체의 합성 및 시험관내 항암 활성

李奉憲,辛貞姬,張太植,朴章洙,姜信源 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

8-Azaxanthine(1), 3-β-D-ribofuranosyl-8-azaxanthine(2),3-β-D-ribofuranosyl-8-azaxanthine-5'-monophosphate(3), 그리고 3-β-D-ribofuranosyl-8-azaxanthine-5'-(3-pyridinylcarbonyl)monophosphate(4)를 합성한 후 Mouse leukemia P388,Murine mammary carcinoma FM3A, Human histiocytic lymphoma U937암세포들에 대해 시험관내 항암활성을 MTT를 이용한 방법으로 예측하고 IC_50(μmol/mL)으로 나타내었다. 그 결과, 비정상적인 염기의 N-3와 당의 C-1'glycoside결합을 가진 2는 세가지의 암세포에 대해 감수성이 좋아 IC_50이 각각 0.05, 0.06,0.06μmol/mL이었으나 항세균 활성 예측에서는 감수성을 보이지 않았다. Human histiocytic lymphoma U937세포에 대한 IC_50은 1이 0.33,2는 0.06,3은0.25그리고 4는 0.33μmol/mL로 구조 변화에 따라 서로 다른 값을 보였다. 8-Azaxanthine(1), 3-β-D-ribofuranosyl-8-azaxanthine(2),3-β-D-ribofuranosyl-8-azaxanthine-5'-monophosphate(3), 3-β-D-ribofuranosyl-8-azaxanthine-5'-(3-pyridinylcarbonyl)monophosphate(4) were synthesized. The in vitro antitumor activities of the synthesized compounds against P388 mouse leukemia,FM3A mammary carcinoma, and U937 human histiocytic lymphoma cells were determined by MTT assay. 2 with unnatural N-3 and C-1'glycoside bond had activityagnist three tumor cell lines and IC_50s of these compounds were 0.05,0.06μmol/mL against three tumor cell lines, repectively. But these compounds had no antibacterial activity. IC_50s aganist U937 human histiocytic lymphoma cells were verfied with the structural modification:IC_50s of 1,2,3, and 4 were 0.33,0.06,0.25, and 0.33μmol/mL, respectively.

Mastoparan B의 항균 활성 및 용혈 작용에 미치는 소수성도와 양친매성의 영향

이봉헌,김광호,장태식,박남규,박장수,강신원 부산대학교 유전공학연구소 1998 분자생물학 연구보 Vol.14 No.-

Tetradecapeptide인 Mastoparan B(MP B)와 이의〔Ala〕-MP B유도체들을 합성하고 항균 활성 및 용혈 작용을 측정하여 MP B의 활성에 미치는 소수성도(hydrophobicity)와 양친매성(amphiphilicity)의 영향을 비교하였다. 그 결과 MP B와 소수성도가 MP B 보다 큰〔Ala^2〕-,〔Ala^4〕-MP B유도체가 큰 항균 활성을 나타내었으며, 소수성도가 MP B보다 작은〔Ala^6〕-MP B유도체의 활성은 MP B와 비슷하거나 작게 나타나 소수성도가 큰 유도체일수록 항균활성을 크게 나타내었다. 그러나 MP B보다 소수성도가 큰 유도체인〔Ala^9〕-MP B는 Ala에 의한 Trp의 치환때문에 MP B보다 작은 활성을 나타내었다.용혈 활성 측정 결과 MP B보다 소수성도가 큰〔Ala^2〕-,〔Ala^4〕-MP B유도체가 각각100.0%와 69.4%로써 용혈 작용을 크게 나타냈으나, 소수성도가 작은〔Ala^6〕-MP B유도체는 6.1%로써 가장 작은 용혈 작용을 나타내었고 Trp대신 Ala으로 치환한 소수성도가 큰 유도체인〔Ala^9〕-MP B는 MP B보다 작은 26.0%의 용혈 작용을 나타내었다. 그러므로 용혈 작용은 소수성도가 클수록 증가하였으며 양친매성이 활성에 미치는 영향은 적었다. Tetradecapeptide.Mastoparan B(MP B)and its〔Ala^2 〕-,〔Ala^4〕-,〔Ala^6〕-,〔Ala^9〕-MP Bderivatives were synthesized, and then their antibacterical and hemolitic were to examine the effect of hydrophobicity and ampiphilicity on the MP B-induced those activities. MP B and more hydrophobic〔Ala^2 〕-,〔Ala^4〕-MP B showed stronger antibacterical activity and less hydrophobic〔Ala^6〕-MP B than MP B did similar or weaker activity, so more hydrophobic〔Ala〕-MP B derivative had stronger activity. But more hydrophobic〔Ala^9〕-MP B than MP B showed weaker activity because of its Trp subsitution by Ala. On the other hand,〔Ala^2 〕-and〔Ala^4〕-MP B showed 100.0% and 69.4% hemoiytic activity, but〔Ala^6〕-MP B did the weakwst activity(6.1%)and〔Ala^9〕-MP B, weaker activity(26.0%) than MP B.Therefore, more hydrophobic〔Ala〕-MP B derviative had stronger activity and the effect of ampiphilicity on the activity was weak.

Mastoparan B의 항균 활성 및 용혈 작용에 미치는 소수성도와 양친매성의 영향

이봉헌,김광호,장태식,박남규,박장수,강신원,Lee, Bong-Hun,Kim, Kwang-Ho,Jang, Tae-Sik,Park, Nam-Kyu,Park, Jang-Su,Kang, Shin-Won 한국미생물학회 1998 미생물학회지 Vol.34 No.1

Tetradecapeptide, Mastoparan B(MP B) and its [$Ala^2$]-, [$Ala^4$]-, [$Ala^6$]-, [$Ala^9$]-MP B derivatives were synthesized, and then their antibacterial and hemolytic activities were assayed to examine the effect of hydrophobicity and amphiphilicity on the MP B-induced those activities. MP B and more hydrophobic [$Ala^2$]-, [$Ala^4$]-MP B showed stronger antibacterial activity and less hydrophobic [$Ala^6$]-MP B than MP B did similar or weaker activity, so more hydrophobic [Ala]-MP B derivative had stronger activity. But more hydrophobic [$Ala^9$]-MP B than MP B showed weaker activity because of its Trp substitution by Ala. On the other hand, [$Ala^2$]- and [$Ala^4$]-MP B showed 100.0% and 69.4% hemolytic activity, but [$Ala^6$]-MP B did the weakest activity(6.1%) and [$Ala^9$]-MP B, weaker activity(26.0%) than MP-B. Therefore, more hydrophobic [Ala]-MP B derivative had stronger activity and the effect of amphiphilicity on the activity was weak. Tetradecapeptide인 Mastoparan B(MP B)와 이의 [Ala]-MP B 유도체들을 합성하고 항균 활성 및 용혈 작용을 측정하여 MP B의 활성에 미치는 소수성도(hydrophobicity)와 양친매성(amphiphilicity)의 영향을 비교하였다. 그 결과 MP B와 소수성도가 MP B 보다 큰 [$Ala^2$]-, [$Ala^4$]-MP B 유도체가 큰 항균 활성을 나타내었으며, 소수성도가 MP B보다 작은 [$Ala^6$]-MP B 유도체의 활성은 MP B와 비슷하거나 작게 나타나 소수성도가 큰 유도체일수록 항균 활성을 크게 나타내었다. 그러나 MP B 보다 소수성도가 큰 유도체인 [$Ala^9$]-MP B는 Ala에 의한 Trp의 치환때문에 MP B 보다 작은 활성을 나타내었다. 용혈 활성 측정 결과 MP B보다 소수성도가 큰 [$Ala^2$]-, [$Ala^4$]-MP B 유도체가 각각 100.0%와 69.4%로써 용혈 작용을 크게 나타냈으나, 소수성도가 작은 [$Ala^6$]-MP B 유도체는 6.1%로써 가장 작은 용혈 작용을 나타내었고 Trp 대신 Ala으로 치환한 소수성도가 큰 유도체인 [$Ala^9$]-MP B는 MP B 보다 작은 26.0%의 용혈 작용을 나타내었다. 그러므로 용혈 작용은 소수성도가 클수록 증가하였으며 양친매성이 활성에 미치는 영향은 적었다.

고등학교 환경과학 교과서의 질문과 문장 내용 분석

이봉헌,문성배,문정대 한국환경과학회 1997 한국환경과학회지 Vol.6 No.3

The question style in high school environmental science textbook was examined in terms of the placement, frequency, and type of question, and then analyzed the kind of scientific inquiry process elicited by the question in the topic of textbook using the Textbook Questioning Strategy Assessment Instrument (TQSAI). The average number of question per topic was only 0.6. The number of all question in the high school environmental science textbook was very little ; the number of non-experiential question was 8 and that of experiential one was 3. The total number of sentence was 1,236 and the ratio of the number of question to that of sentence was 0.9%. The frequency of non-experiential question was higher than that of experiential one. In action part of the textbook, there were more kinds of question styles than in the main part.

해양 V. vulnificus의 내독소가 rat의 혈액 성분에 미치는 독성 효과

이봉헌 한국환경과학회 1997 한국환경과학회지 Vol.6 No.1

Endotoxin from the cell wall of marine V. vulnificus was extracted using the hot phenol-water method, injected endotoxin into rat, and tested the toxic effect of endotoxin on the blood component in rat blood. The results showed that blood glucose, blood urea nitrogen, white blood cell, and reticulocyte were increased and red blood cell was the same as the number of control group(normal blood), but platelet was decreased. Above results suggested that endotoxin induced a malfunction of liver and that the increase of white blood cell was for the removal of foreign toxic substance.