Sphingosine 1-Phosphate and Sphingosine Kinase Activity during Chicken Embryonic Development

Choi, Chang-Hwan,Jeong, Ji-Seon,Yoo, Bo-Im,Jin, You-Xun,Moon, Dong-Cheul,Yoo, Hwan-Soo,Oh, Seik-Wan,Hong, Seon-Pyo,Lee, Yong-Moon 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.4

The chicken embryo has been weil used in studies of the developmental process, and during development sphingosine and sphingosine 1-phosphate (So1P) are considered critical mediators of cell death and survival. In this study, we compared the sphingolipid contents of chicken embryos during the early embryonic development period from day 3 to day 6. HPLC analyses of sphingosine and So1P in chicken embryos revealed that sphingosine levels were greatly reduced on day 4 whereas So1P levels were not significantly changed. Sphingosine kinase(Sphk) activities, which require sphingosine as substrate to produce So1P, were also greatly reduced on day 4. Collectively, we found sphingosine levels and Sphk activities, but not So1P levels are changed in early stage of chicken embryos development.

Control of Reversible Self-Bending Behavior in Responsive Janus Microstrips

Oh, Myung Seok,Song, Young Shin,Kim, Cheolgyu,Kim, Jongmin,You, Jae Bem,Kim, Taek-Soo,Lee, Chang-Soo,Im, Sung Gap American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.13

<P>Here, we demonstrate a simple method to systematically control the responsive self-bending behavior of Janus hydrogel microstrips consisting of a polymeric bilayer with a high modulus contrast. The Janus hydrogel microstrips could be easily fabricated by a simple micromolding technique combined with an initiated chemical vapor deposition (iCVD) coating, providing high flexibility in controlling the physical and chemical properties of the microstrips. The fabricated Janus hydrogel microstrip is composed of a soft, pH-responsive polymer hydrogel layer laminated with a highly cross-linked, rigid thin film, generating a geometric anisotropy at a micron scale. The large difference in the elastic moduli between the two layers of the Janus microstrips leads to a self-bending behavior in response to the pH change. More specifically, the impact of the physical and chemical properties of the microstrip on the self-bending phenomena was systematically investigated by changing the thickness and composition of two layers of the microstrip, which renders high controllability in bending of the microstrips. The curvature of the Janus microstrips, formed by self-bending, highly depends on the applied acidity. A reversible, responsive self-bending/ unbending exhibits a perfect resilience pattern with repeated changes in pH for 5 cycles. We envision that the Janus microstrips can be engineered to form complex 3D microstructures applicable to various fields such as soft robotics, scaffolds, and drug delivery. The reliable responsive behaviors obtained from the systematic investigation will provide critical information in bridging the gap between the theoretical mechanical analysis and the chemical properties to achieve micron-scale soft robotics.</P>

일부 한국인의 혈청 ceruloplasmin 정상 참고치 설정 및 질환별 비교 : Comparison of Its Level among Various Diseases

이동수,박애자,박정덕,장임원,홍연표 중앙대학교 의과대학 의과학연구소 1999 中央醫大誌 Vol.24 No.1

Ceruloplasmin has been investigated as one of the useful biologic index for early diagnosis of atherolsclerosis. However, there was no normal reference value taking into account the confounders in Korea. This study was performed to estimate the normal reference values and present the confounders of ceruloplasmin. Also we compared of serum ceruloplasmin levels among normal reference group and three abnormal groups: 475 healthy subjects, 53 liver dysfunctions, 72 positive test of chronic inflammatory diseases, and 89 hypercholesterolemias. Mean serum ceruloplasmin level of healthy subjects were 41.6±7.6㎎/㎗ in males and 42.1±7.2㎎/㎗ in females, respectively. It was tend to increase of ceruloplasmin by age in both sexes. However, the statistical difference was not found by age group between both sexes. Statistically significant correlations were found between serum ceruloplasmin and age, body mass index(BMI), white blood cell(WBC) and triglyceride(TG) in males and age, BMI, WBC and total cholestrol(TG) in females. After adjustment for age, BMI,WBC and TG in males and age, BMI, WBC and TG in females, significant elevations of serum ceruloplasmin were found in liver dysfunction(p=0.0077) and positive test of chonic inflammatory disease(p=0.0217) in males and in oisitive test of chronic inflammatory disease(p=0.0023) and hypercholestolemia(p=0.0124) in females relative to healthy reference subjects. There is no statistical difference among liver dysfunction, postive test of chronic inflammatory disease, and hypercholesterolemia.

Classical Swine Fever (Hog cholera) Virus 약독순화주 (Suri 주)의 gp55 Gene 염기서열 분석

김귀현,장경수,강경임,이병형,박종현,안수환,전무형 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

An attenuated classical swine fever virus (CSFV), Suri strain, is a variant derived from a vaccine virus, LOM strain. This study was performed to elucidate the molecular biologcal properties of CSFV Suri strain, and to obtain the basic data for molecular epidemiological approaches for the disease. The truncated form of gp55 gene without the C-terminal trasmembrane domain, in size of 1,023bp, was amplified by RT-PCR and sequenced by dye terminator cyclic sequencing method, and inserted into BamHI site of pAcGP67B baculovirus vector, establishing a cloned pAcHEG plasmid. By the nucleotide sequences determined, 341 amino acid sequences were predicted. As compard the nucleotide and amino acid sequences of gp55 of Suri with the various CSFV, Suri strain showed the high homology over 99.1% with ALD and LOM strains, but comparably the lower homology with Alfort and Brescia. In comparison of amino acid sequence in variable domain of gp55 protein, the similar tendency of homology was observed. In hydrophobicity analysis, all of four CSFV strains revealed the analogous patterns of hydrophobicity. The numbers and locations of N-glycosylation site and cysteine residuse in gp55 were analyzed, those of Suri strain being coincident with ALD and LOM strains. The results suggest that gp55 in Suri strain has the high similarity to those in ALD and LOM strains in terms of the nucleotide and amino acid sequences and the functional properties of gp55 protein.

하청 근로자들의 건강수준 평가

최홍열,고상백,장세진,차봉석,임형준,이상윤,김재용,강동묵,조수헌 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.1

목 적 : 하청노동을 이용한 노동 유연화 전략은 근로자들의 고용을 불안정시키고, 근로자들의 삶에 부정적 영향을 미치고 있다. 따라서 이 연구는 하청업체 근로자들의 고용현황을 조사하고, 기존의 업체중심의 건강평가의 문제점을 지적하고자 하였다. 또한 하청업체 근로자들의 건강평가의 현재적 의미와 사회적 배제의 특성을 알아보고자 하였다. 방 법 : 모기업과 하청업체 근로자들의 건강수준을 비교하기 위하여, 정기건강진단(일반, 특수)과 채용건강진단 자료를 이용하고자 하였다. 건강진단에서 누락된 비정규직을 포괄하기 위하여 SF-36을 이용하여 건강설문조사를 시행하였다.결 과 : 조선업 하청업체 근로자들의 고용기간은 짧고, 빈번한 노동이동 현상을 보였다. 3년간 건강진단 수검 현황을 통해 살펴볼 때, 1998년 수검자중 2000년까지 연속해서 검진 받은 수검자는 40.3%였다. 건강진단 결과 유소견자 규모를 통해 모기업과 하청업체간의 건강수준을 비교해 보았다. 모기업은 일반질병 요관찰자와 질병자를 포함한 유소견자가 1,011명으로 약 10 %의 유소견율을 보이고 있고, 질병자의 경우 596명으로 유병률이 5 %를 상회하고 있다. 반면 하청업체의 경우 1,967명의 수검자중 유소견자가 115명으로 6 %의 유소견율을 보이고있고, 질병자는 82명으로 4 %의 유병률을 보이고있다 채용건강진단을 받은 수검자는 6,260명 이었으며, 이 중 유소견자 수는 2,373명으로 전체 수검자의 37.9 %를 차지하였다. 건강설문지의 경우는 전체 건강수준의 총합은 하청보다 원청 근로자가 높았고 통계적으로 유의하였다. 결 론' 정기건강검진 결과는 하청근로자들이 고용형태에 따라 검진을 받지 못한 경우가 많아, 건강 근로자 효과로 표현될 가능성이 있다. 따라서 채용건강진단 결과는 다른 측면에서 하청업체 근로자들의 건강수준의 현실적 반영이라고 할 수 있다. 향후 하청업체 근로자들의 건강문제를 감시하고 개선하려면 기존의 업체 중심의 접근으로는 하청 근로자의 건강문제를 제대로 다룰 수 없고, 업종과 지역을 동시에 고려하여 접근할 필요가 있다. 또한 단기 고용 근로자 및 비정규직 근로자를 사회보장의 확충과 모기업의 연대책임 강화 및 기업복지 차원에서 포괄하는 방안이 모색되어야 하겠다. Objectives : The strategy for the labor flexibility through subcontracted labor have brought a negative effect on the lives of the workers, such as the increase of the unstable employment. This study was conducted to analyze the relationship between the employment of subcontracted firms and the health status of them, and to find out some problems which have been caused in the process of health evaluation and the characteristics of social exclusion. Methods : We reviewed the periodical health examination and preemployment health examination in order to compare health status of the subcontracted workers with those of the parent firm workers. The SF-36 questionnaire was administered to assess the self-perceived health status. Results : The result shows that compared to the working duration of the parent firm workers, those of the subcontracted workers were shorter, and the job transfer rate was higher. In the periodical health examination, the prevalence of occupational and general disease was higher in the parent firm(10%) than in the subcontracted firms(6%). Preemployment health examination plays an important role in worker's health manage- ment. Among 6,260 workers who received preemployment health examination, the prevalence of disease was 37.9%. Mean scores of health status measured by SF-36 were lower in the subcontracted firm than in the parent firm. Conclusions : Due to social exclusion of the subcontracted workers from the periodical health examination, there is a possibility that the periodical health examination have the healthy worker effect. These results suggests that the preemployment health examination could be a more appropriate indicator which is able to assess the health status of the subcontracted workers than the periodical health examination.

건강보험에서 5인 미만 사업장의 직장보험 편입 효과 평가와 정책 방향

송명섭,김창보,고수경,임정수,박지연 한국사회보장학회 2002 사회보장연구 Vol.18 No.2

본 논문은 건강보험공단이 2001년 「월 이후 추진하고 있는 5인 미만 사려장의 직장보험 편입을 평가하고 향후 정책방향을 제시하는데 목적을 두고 있다. 5인 미만 사업장의 직장보험 편입은 보험 가입자의 보험료 부과의 형평성을 제고하고, 영세사업장 노동자들의 보험료 부담을 경감시키며 건강보험재정 안정 효과를 위한 것이다. 2001년 7월 이후 12월 31일까지 직장보험으로 신규편입한 대상자는 모두 약 64만명이었으나 편입이전지역보험 대상자는 약 53만명, 직장보험 대상자는 약 10만명이었다. 또한 동일 기간동안 직장보험으로 편입된 사업장은 모두 88,751개였다. 편입 전후 개인별 보험료 수준 변화를 살펴보면 보험료 부과대상 중 77.6%는 본인이 부담해야 할 보험료가 감소하였다. 대신 사업주의 보험료 부담은 상대적으로 늘어났다. 한편 2001년 7월 이후 12월까지 직장보험 편입으로 건강보험의 재정은 약 19,530백만원의 재정절감효과가 있었다. 직장보험은 7,331백만원의 보험료 수입 증가가 발생하였으며, 지역보험은 12,199백만원의 재정절감 효과가 있었다. 향후 5인 미만 사업장의 직장보험 편입이 원만히 추진되기 위해서는 피부양자 인정기준의 개선과 영세사업장 사업주의 보험료 부담을 경감시켜주기 위한 방안을 강구해야 하며, 사회적으로는 비정규직 노동자에 대한 사회보장 적용 확대를 위한 노력이 동시에 추구되어야 할 것이다. This study was conducted for the purpose of evaluating coverage of small companies with less than 5 persons by Corporate Insurance since July 2001 and of suggesting policy implication. This coverage expansion by Corporate Insurance was intended for improving equity level of contribution imposition, alleviating financial burden of employees and achieving financial stability of health insurance. While, from July 2001 to December, the number of newly covered beneficiaries was about 642 thousand persons, the number of Regional beneficiaries before the new coverage expansion by Corporate Insurance was 533 thousand persons. And the number of Corporate Insurance beneficiaries before the coverage expansion was 101 thousand persons. For the same period, the number of companies by the new coverage expansion of Corporate Insurance was 88,751. Before and after the expansion, taking into account changes of individual contribution levels, payment share levels of contribution were reduced to the 77.6% of the beneficiaries newly covered by Corporate Insurance. Instead, payment share level of contribution of employers was increased more than before. And 19,530 million won was increased in National Health Insurance Expenditure. 7,331million won was increased in Corporate Insurance revenue. Expenditure reduction effect of 12,199 million won was able to be obtained in Regional Insurance. In order to implement successfully the coverage expansion by Corporate Insurance in times ahead, employers' contribution share should be reduced, when considering low profitability levels of small companies.

新入生 意識構造의 調査를 기초로 한 學生 生活의 새로운 指導方法

최완식,전창수,윤정임 한국항공대학교 1993 論文集 Vol.31 No.-

이 硏究는 1992학년도의 한국항공대학교 신입생 의식구조의 조사를 토대로 한 새로운 학생생활지도 방안을 設定하기 위해서 마련된 것이다. 특히 이 연구의 진행과정에서는 최근 東北아시아의 국제정치 및 경제사정등의 극적인 變化가 학생생활에 미치는 영향 도 깊이 고려하였다. 뿐만 아니라 지난 오랜동안 한국항공대학교가 위치한 이 캠퍼스지역에 가로놓인 많은 어려운 장애 물과 악조건들이 학생들의 학습활동이나 연구생활에 미치는 악영향등도 충분히 유의하였다. 이 硏究의 두번째 목적은 무엇보다도 학생들의 進路지도를 적극 강화하고 보완하는 방안이 생활의 勉學분위기를 조성 하고 활기찬 학습 및 연구생활에 더욱 크게 이바지 하게 될 것이라는 점을 크게 유념하였다. This study is to eatablish "New Directions of Guidance for Students Life on the Basis of Research into Freshmen's Way of Thinking(1992)" in Hankuk Aviation University. In the process of this research, Particularly, we have deeply considered into the great influence upon the students life from the recent dramatic change of international polities and economic circumstances of Northeastern Asian countries. On the other hand, we have kept in mind very difficult conditions and circumstances laid in this campus area which have greatly influenced upon the very hard and inconvenient studing life of students for a long time past. The second objective of this research is to study of New Directions of Guidance for Making New job of Graduates and future new plan for variable social life after graduation. We believe such methods of New Guidance will remarkably devote on the effective and industrious studing life of students of Hankuk Avation University.

전형적인 DISIDA scan 소견의 Rotor 증후군 1예

황의태,염동한,권지혜,임종주,최창수,김태현,김학철 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

만성의 비용혈성 직접형 고빌리루빈혈증은 Rotor 증후군과 Dubin-Johnson 증후군의 특징적인 소견으로, Rotor 증후군은 드문 양성 유전성 질환이며, Dubin-Johnson 증후군과 감별을 필요로 한다. 본 저자들은 황달을 주소로 내원한 26세 남자 환자에서 고빌리루빈혈증에 대한 검사를 시행하여 담도폐쇄 소견없이 간담도 스캔상 전형적인 Rotor 증후군의 소견 즉, 강한 심장 배후방사능이 지속되면서, 간담도계의 조영은 지연 촬영에서도 관찰되지 않으며 신장으로의 배설이 계속 관찰되는 특징적인 소견과 정상 간조직 검사 결과로 Rotor 증후군으로 진단한 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Chronic nonhemolytic conjugated hyperbilirubinemia characterizes both Rotor and Dubin-Johnson syndromes. Rotor syndrome is a rare, benign familial disorder and needs to be differentiated from Dubin-Johnson syndrome. Cholescintigraphy is a simple and informative procedure for the diagnosis, which can differentiate Rotor syndrome from Dubin-Johnson syndrome. A 26-year-old man was admitted due to asymptomatic persistent jaundice. Physical examination revealed icteric sclera without hepatosplenomegaly. Laboratory findings showed increased serum bilirubin with direct bilirubinemia. Markedly decreased hepatic uptake and poor visualization of the gallbladder and biliary tract were shown in ^(99m)Tc-DISIDA scan. Histology of the liver showed no diagnostic abnormality without pigmentation. But, we report a case of Rotor syndrome that showed characteristic cholescintigraphic findings with normal urinary total coproporphyrin excretion.

감귤류 종자 추출물의 N-nitrosodimethylamine 생성억제에 관한 연구

오혁수,안용석,나임순,오명철,오창경,김수현 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.5

The effect of inhibition on N-nitrosodimethylamine (NDMA) formation by methanol extracts from 6 kinds of citrus seed (Citrus sunki, Citrus natsudaidai, Citrus suleata, Citrus tangerina, Citrus grandis and Citrus obovoidea) were investigated. The contents of moisture, crude protein, crude fat, carbohydrate, ash, flavonoid and totat phenol in citrus seeds were 4~6, l1~15, 32~46, 22~45, 2~4%, 12~24mg% and 53~133㎎%, respectively. The solid contents and yield of citrus seed extracts were 0.8~1.0 and 0.7~1.1%, respectively. Nitrite-scavenging activity by methanol extracts from citrus seeds increased with increasing extract dosage. Furthermore, the nitrite scavenging activity was pH dependent being the highest at pH 1.2 (42.7~96.9%) and the lowest at pH 6.0 (19.9~62.6%). Scavenging effects of nitrite by reaction time showed high effects under 3hr reaction time. The inhibition effect of NDMA formation by the citrus seed was strengthened as the amount of extract increased. The inhibition rate of methanol extracts from citrus seeds on NDMA formation showed 1.2~39.8%, 21.3~60.1% and 47.4~94.0%, according to add 1, 3 and 5㎖, respectively. Therefore, the inhibition effect of NDMA formation by the citrus seed was strengthened as the concentration of extract increased.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.