Apoptosis and necroptosis‑inducing effects of arctigenin on nasal septum carcinoma RPMI‑2650 cells in 2D and 3D culture

Yoon‑Jin Lee,Kwan‑Sik Park,Byoung Joon Baek,Kyong‑Ae Lee,Sang‑Han Lee 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.1

Backgrounds Arctigenin derived from the seeds of Arctium lappa Linnaeus is known as an anticancer drug candidate by targeting various pathways involved in anticancer therapy. Methods Using 2D monolayer and 3D spheroid culture systems in nasal septum carcinoma RPMI-2650 cells, the effects of arctigenin and dexamethasone on cell viability, ROS levels, ATP level, mitochondrial function, apoptosis and necroptosis were examined. Results The combination treatment of both compounds induced strong cytotoxicity, accompanied by increases of sub-G0/ G1 peak, annexin V-PE-positive cells, and ROS levels, loss of mitochondrial membrane potential, and decrease of cellular ATP content. These changes were observed as simultaneous induction of DNA damage, apoptosis, and necroptosis. A series of changes by arctigenin and dexamethasone were efficiently restored by decreasing ROS levels or supplementing ATP. Treatment of 3D spheroids with arctigenin and dexamethasone decreased cell viability in the spheroids, but it was slightly resistant than cells under 2D conditions. In addition, this phenomenon was accompanied by an increase in mediators for both apoptosis and necroptosis. Conclusion Results of this study suggest that the apoptosis and necroptosis-inducing effects of arctigenin are associated with ATP depletion due to oxidative mitochondrial dysfunction.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

3D-CT를 이용한 골격성 III급 개방교합자의 악교정 수술 전, 후 설골 및 상기도의 변화

이윤섭,백형선,이기준,유형석 대한치과교정학회 2009 대한치과교정학회지 Vol.39 No.2

본 연구는 골격성 III급 개방교합자의 악교정 수술 전, 후 설골과 상기도의 변화를 3D-CT를 이용하여 관찰하고 이를 정상교합자와 비교하기 위하여 시행하였다. 전치부 개방교합을 동반한 골격성 III급 부정교합으로 진단되어 악교정 수술을 받은 환자 중 12명을 선정 후, 3D-CT를 통해 설골과 상기도의 3차원 입체영상을 분석하여, 악교정 수술 전, 후의 설골의 위치 변화 및 상기도의 부피 변화를 살펴보고, 이의 결과를 정상교합자 10명과 비교하였다. 설골은 골격성 III급 개방교합군에서 악교정 수술 전에 정상교합군의 설골에 비해 전방에 위치하고 있었고 악교정 수술 후 설골은 후, 상방으로 변화하였으나 유의성은 관찰되지 않았다. Hyoid plane과 mandibular plane이 이루는 각은 악교정 수술 전의 골격성 III급 개방교합군이 정상교합군보다 큰 값을 나타내었고, 악교정 수술 후에는 그 차이가 더욱 증가하였다. 골격성 III급 개방교합군의 상기도 부피는 정상교합자보다 작으며 이는, 악교정수술 후에 더욱 감소하였다. 3차원 영상 분석 결과, 골격성 III급 개방교합자의 상기도는 정상교합자에 비해 좁으며, 하악골 후퇴술 이후 더욱 감소하기 때문에 이는 수술적 개선의 안정성에 영향을 줄 수 있음을 알 수 있었다. Objective: The purpose of this study was to investigate the structural changes of the hyoid bone and upper airway after orthognathic surgery for skeletal class III anterior open bite patients, and make comparisons with normal occlusion. Methods: Pre- and post-operative computed tomography (CT) examinations were performed on 12 skeletal class III anterior open bite patients who were treated with mandibular setback osteotomy. Using the V-works 4.0 program, 3-dimensional images of the total skull, mandible, hyoid bone, and upper airway were evaluated. Results: In the Class III openbite group, the hyoid bone were all positioned anteriorly, compared to the Normal group (p < 0.05). The angle between the hyoid plane and mandibular plane in the Class III openbite group before surgery was greater than in the Normal group (p < 0.05), and the difference increased after surgery (p < 0.01). In the Class III openbite group, the volume of the upper airway decreased after surgery (p < 0.001) and the volume of the upper airway was smaller than the Normal group before and after surgery (p < 0.001). Conclusions: The narrow upper airway space in skeletal Class III openbite patients decreased after mandibular setback osteotomy. This may affect the post-surgical stability.

음향방출을 이용한 콘크리트 부재의 미시적 파괴특성의 온라인 모니터링

윤동진,이진경,이준현 韓國非破壞檢査學會 1999 한국비파괴검사학회지 Vol.19 No.1

콘크리트는 다상(multi-phase)의 재료로서 구성되어지는 복합재료의 일종으로써 준취성(quasi-brittle)적인 재료적 특성을 가지고 있기 때문에 실제 복잡한 미시적 파괴특성을 나타낸다. 따라서 콘크리트 구조물의 안전성 확보를 위해서는 먼저 하중의 증가에 따른 콘크리트 내부의 변형특성 및 미시적 파손기구를 파악하여야 하며, 특히 실제 구조물에 있어서는 이들 특성들을 비파괴적으로 상시(on-line) 모니터링 하여야 할 필요가 있다. 따라서 본 연구에서는 3점 굽힘 하중을 받는 콘크리트 부재의 미시적 파손기구 및 각 파손기구에 대한 AE 특성을 명확히 함으로써 콘크리트 부재의 미시적 손상 및 파괴 특성을 비파괴적으로 평가하고 AE 발생원 위치 추정 기법을 이용하여 균열 발생 및 진전거동을 평가하였다. Since concrete is an inhomogeneous material consisting of larger aggregates and sand embedded in a cement paste matrix, it relatively shows a complex failure mechanism. In order to assure the reliability of concrete structure, microscopic fracture behavior and internal damage progress of concrete under the loading should be fully understood. In this study, an acoustic emission(AE) technique has been used to clarify microscopic failure mechanism and their corresponding AE signal characteristics of concrete under three-point bending test. In addition 2-dimensional AE source location has been performed to monitor the progress of an internal damage and the successive crack growth behavior during the loading. The relationship between AE signal characteristics and microscopic fracture mechanism is discussed.

골형성부전증 10예의 임상적 특징

이형숙,김현주,조재현,이승원,김현아,최준혁,송영준,김대중,이관우,정윤석 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.5

연구배경 및 방법: 골형성부전증은 비교적 희귀한 유전병으로 교원질 대사 장애로 인한 골의 취약성과 다발성 골절 및 척추측만증 등을 특징으로 한다. 유전방식과 표현형의 발현 정도에 따라 다양한 임상 양상을 보이며, 임상적 중증도에 따라 4가지 형태로 분류된다. 지금까지 국내 보고는 분만과정이나 태아 진찰시 골격이상으로 발견된 증례보고가 주였다. 저자들은 비교적 경미한 임상 양상을 보이는 예를 포함한 다수의 골형성부전증 환자들의 전반적인 임상적 특징에 대해 보고하는 바이다. 결과: 2001년 6월부터 2003년 2월까지 골형성부전증으로 진단받은 6 가계, 10예를 대상으로 하였다. 평균 나이는 27.3(5∼56)세였고 소아가 2예였다. 모두 상염색체 우성으로 유전되었으며, 제 I형이 4예, 제 III형이 4예, 그리고 제 IV형이 2예였다. 전 예에서 다수의 골절 경험이 있었고, 골밀도 저하와 골피질 두께 감소 소견이 관찰되었다. 전신의 평균 골밀도는 0.690(0.421∼1.039) g/cm²였다. 골형성지표로 측정된 sAlk는 소아의 경우만 증가되어 있었고, 골흡수지표로 측정된 uDPD의 평균치는 12.9(4.4∼36.3) nM/mM Cr으로 증가된 소견을 보였다. mobility score는 대부분 3,4단계에 속해 있었다. 중증형일수록 진단 시의 mobility score가 낮은 경향을 보였다. 결론: 한국인 골형성부전증 환자의 임상적 특징을 살펴본 결과 기존의 보고된 II형 외에도 I, III, IV형이 다양하게 존재함을 알 수 있었으며, 모든 예에서 상염색체 우성으로 유전됨을 확인할 수 있었다. 또한 모든 예에서 증가된 골흡수로 인한 골밀도 저하와 골절을 확인할 수 있었다. Osteogenesis Imperfecta (OI) is a relatively rare hereditary disease, which is characterized by multiple bone fractures and spine scoliosis, due to the fragility of bone, and is often associated with blue sclerae, deafness and dentinogenesis imperfecta. Four types of OI can be distinguished, according to the clinical findings. Although mutations affecting type I collagen are responsible for the disease in most patients, the mechanism by which the genetic defects cause abnormal bone development remains to be fully understood. Here, the clinical characteristics of 10 OI patient cases are reported, with a review of the literature. All the cases, including 4 type I, 4 type III and 2 type IV, inherited OI as an autosomal dominant trait. All the subjects had multiple old fractures and decreased bone densities. In this study, the biochemical marker of bone formation, serum alkaline phosphatase, was found to be increased only in the pediatric OI patients, while the biochemical marker of bone resorption, urinary deoxypyridinoline, was increased in all cases. The mobility score was found to correlate with the severity of the type on diagnosis (J Kor Soc Endocrinol 18:496∼503, 2003).

시간 경과에 따른 카르복시헤모글로빈 농도 변화에 관한 연구

이윤성,김준호,이정빈 大韓法醫學會 1989 대한법의학회지 Vol.13 No.1

The diagnosis of carbon monoxide (CO) poisoning in clinics and forensic practice largely rests on the determination of carboxyhemoglobin (HbCO) in blood. It is generally known that HbCO remains unchanged gor a couple of days, and such a delay in determination of HbCO may be acceptable. However, there were some incidences of prolonged delay because of initial lack of concern to CO poisoning and other limitations on postmortem examination. The question may be raised on that the prolonged delay would be acceptable for the diagnosis of original CO poisoning. The study was preformed to obtain the changes of HbCO concentration in vitro. Fifteen rabbits were intoxicated with CO in the system which was made on purpose. Initial levels of hemoglobin (Hb) and HbCO were determined by CO-Oximeter (IL 182). And the samples were stored in the refrigerator and in room temperature for 1 and 2 months to check same parameters. The HbCO decreased 5.85±8.37% at one month in room temperature (p<0.01). Meanwhile, after 1 month storage, the HbCO levels of the bloods stored in room temperature were lower as much 4.37±8.24% than those in the refrigerator. The results indicates that the diagnosis of CO poisoning could be obtained after 2 months or later in refrigerated blood, and 1 month after storage in room temperature. It is presumed that the low levels of HbCO in stored blood are partly because of the decreased combining capacity of hemoglobin to CO, which is affected by temperature. And, if the blood is determined for toxic level of CO poisoning after some delay, it should be considered that there is a presumable decrease in HbCO concentration according to the storage temperature.

Study on the Automatization of the Steam Generator Water Level at Low Power

李潤俊 제주대학교 1990 논문집 Vol.31 No.-

원자력 발전소의 증기발생기는 열수력학적 특성에 의하여 저출력에서 수위의 조절이 매우 어려우며, 가동시와 같은 저출력에서 원자로 강제정지의 큰 원인이 되고 있어 경제성 및 안정성에 영향을 미치고 있다. 본고에서는 강제정지 감소 방안의 일환으로 수위 제어의 자동화 영역을 저출력에까지 확장하였으며, 이를 위해 우선 증기발생기의 모델을 수정하여 주요인자에 대한 증기발생기 수위의 거동을 파악하였으며, 이를 위해 우선 증기발생기의 모델을 수정하여 주요인자에 대한 증기발생기 수위의 거동을 파악하였다. 이들 열수력학적 거동을 전달함수형태로 근사시킨후 전체 제어 시스템의 안정도 및 제어특성을 살폈으며, 제어기의 상수를 출력별로 조절함으로써 자동화 영역이 저출력까지 확장될 수 있음을 보였다.

Olanzapine 복용 이후 당뇨병 케톤산증이 발생한 정신분열병 환자

이중선,김진용,안준호,김창윤 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.1

The use of olanzapine has been associated with clinically significant hyperglycemia (exacerbation of existing diabetes, new-onset type 2 diabetes and diabetic ketoacidosis). We report a case of diabetic ketoacidosis (DKA) in 30-year-o1d male patient with schizophrenia who was on olanzapine. When he was admitted to the hospital because of DKA, he had been taking olanzapine for 43 months. 14 months before the admission, his fasting and 2 hour postprandial serum glucose levels were normal. He had no personal and family history of diabetes mellitus. He had no risk factor for diabetes mellitus except for obesity (body mass index : 39.2 kgW). He also hadno precipitating factors for DKA such as infection or myocardial infarction. Periodic monitoring for changes in serum glucose levels in patients taking olanzapine may be needed.

中醫治驗例를 中心으로 살펴본 癌性 疼痛의 外治法

李潤熙,申珉圭,卞俊晳 대한한방종양학회 2000 대한한방종양학회지 Vol.6 No.1

Carcinous pain is severe and continuous so difficult to alleviate. Western drugs give rise to reation such as anoresia, constipation, voniting, general weakness and dyspnea, And they cause tolerence. External treatment is a way to put a patch or rub a liquid on the skin. The patch and liquid are made from herb medicine. In china, they have a good effect of alleviating carcinous pain to make use of external treatment. Usually they use the medicine with effect of reprecussion(消腫)removing obstruction(散結), activating blood flow and removing blood stasis (活血化瘀)such like Borneloum(水片). Bufonis Venenum (蟾?), Aconiti Ciliane Tubber(草鳥), Arisaematis Rhizoma(南星), Asari herba cum Radice (細辛)etc. We can get the alleviating effect more quickly and prevent reaction through extermal treatment.