서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Claudin-1 expression decreases with increasing pathologic grade in actinic keratosis and may be used as a marker of high-risk actinic keratosis

( Ji Su Lee ),( Hyun-sun Park ),( Hyun-sun Yoon ),( Soyun Cho ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: Actinic keratosis (AK) is a common sun-induced skin disorder of importance for the progression to invasive squamous cell carcinoma. However, there is still no good way to predict high risk AK. Objectives: To identify markers which reflect the biologic behavior of AK and to understand the pathogenesis of AK Methods: A total of 52 patients with AK and site-matched 17 normal controls were included. We evaluated solar elastosis and immunohistochemical features using the following antibodies: p53, vitamin D receptor (VDR), claudin-1, and Langerin. Results: Solar elastosis increased and Langerhans cell (LC) density decreased with aging in both patients and controls. Solar elastosis and p53 expression were higher and VDR expression was lower in patients than controls; however, they showed no statistical difference in relation to the pathologic grade of AK. Claudin-1 expression gradually decreased from normal control to severe AK and decreased in the areas with epidermal atypia. LC density in severe AK was significantly lower than in normal control and mild AK; no difference in LC density was seen among control, mild AK and moderate AK. Conclusion: Claudin-1 can be a useful marker of pathologic severity of AK. In contrast, p53 increases and VDR decreases in AK not in gradual manner but in the early steps of carcinogenesis. LC density is relatively maintained in AK until it reaches severe dysplasia.

Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

가축에서 분리된 Vancomycin 내성 장구균의 내성유전자 구조 분석을 통한 분자생물학적 역학 연구

허지영,이선민,이석호,이위교,용동은,이경원,신완식,이동건 대한임상미샐물학회 2003 Annals of clinical microbiology Vol.6 No.2

Hairless Mice를 이용한 광노화 모델에서 APB-01의 경구반복투여에 의한 피부주름개선 효과 시험

이지해(Ji-Hae Lee),이병석(Byung-Suk Lee),변범선(Bum-Sun Byun),김완기(Wan-Gi Kim),이상준(Sang-Jun Lee),심영철(Young-Chul Sim),김배환(Bae-Hwan Kim) 한국독성학회 2003 Toxicological Research Vol.19 No.4

Ultraviolet (UV) is thought to induce erythema, sun-burn, photo-toxicity, photo-allergy, photo-aging and sometimes skin tumor. To investigate the photo-protective effects of APB-01 (Amore-<br/> Pacific Beauty-01, the mixture of Jaummi-dan and Fujiflavone P10) on UV-induced skin damage, forty of SKH hairless female mice were orally administered with APB-01 or saline fifth a week, and irradiated with UV third a week for up to ten weeks. We examined the relationship between visible changes and skin damage in the dermis and epidermis. In the APB-01 treated group, a better skin<br/> and less wrinkles formation were observed when compared to the UV control group. This results demonstrated that oral administration of APB-01 seems to have photo-protective effects on UV-induced<br/> skin damage of hairless mice due to an inhibitory effect on collagen breakdown, and the model using hairless mice is very useful to investigate the efficacy of functional beauty foods.

GC-ECD를 이용한 유기염소계 및 Pyrethroid계 농약 동시 분석법

김우성,이선화,김재이,정지윤,이명자,박영채,이영자,정성욱,이봉헌,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with florisil, and finally analyzed simultaneously the organochlorine and pyrethroid pesticides using GC(ECD). An ultra-2 fused silica capillary column was used to separate and identify the products. The resolution between the last isomeric peak of cypermethrin(59.987min) and the first isomeric peak of flucythrinate(60.043min) was not satisfactory. The last isomeric peak of fenvalerate(62.344min) and the first isomeric peak of fluvalinate(62.397min) were overlapped. Recoveries of soybean sample for the most pesticides were 73.3% to 102.4%. Detection limits were between 0.004 and 0.063 ㎍/㎎ when this method was used.

중추성 요붕증이 동반된 랑게르한스 세포 조직구증 1예

김진호,문준성,문선중,이지은,최재원,은미정,천경아,조인호,윤지성,원규장,이경희 신덕섭,이형우 영남대학교 의과대학 2005 Yeungnam University Journal of Medicine Vol.22 No.2

Central diabetes insipidus (DI) is a syndrome characterized by thirst, polydipsia and polyuria. Langerhans cell histiocytosis is one of the etiologies of DI. Recently we experienced a central DI associated with Langerhans cell histiocytosis. The 44 years old female patient complained right hip pain polydipsia and polyuria. We carried out water deprivation test. After vasopressin injection, urine osmotic pressure was increased form 109mOsmol/Kg to 327mOsmol/Kg (300%). Brain MRI showed a thickened pituitary stalk and at hot bone CT.CT guided biopsy revealed abnormal histiocytes proliferation and abundant lymphocytes, The final diagnosis was central DI associated with systemic Langerhans cell histiocytosis invading hip bone, L-spine and pituitary stalk. Desmopressin and etoposide chemotherapy were performed to the patient.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

급성 심근경색증을 동반한 혈전성 혈소판 감소성 자반증 1예

이태희,김진혁,김완중,박지영,강흥선,조정휘,김권삼,송정상,배종화 대한순환기학회 2003 Korean Circulation Journal Vol.33 No.11

교사가 인식하는 특성화 고등학교 교육의 문제점

이용환,금지헌,안선영 한국농업교육학회 2007 농업교육과 인적자원개발 Vol.39 No.4

이 연구의 목적은 특성화 고등학교에 근무하는 교사가 특성화 고등학교에 근무하면서 인식하는 문제점을 파악하고,특성화 고둥학교가 나아가야할 방향을 제시하는데 있다. 이 연구는 교사가 인식하는 문제점을 파악하기 위해 델파이 방법으로 연구되었다. 델파이 패널은 특성화 고등학교로 신설 및 전환된 지 5년 이상 된 학교의 특성화 또는 실과 부장 교사 18명으로 구성되었다. 3차의 델파이 조사가 이루어 졌다. 1차 델파이 질문지는 개방형으로 교사가 인식하는 특성화 고등학교의 문제점을 자유롭게 기술하도록 하였다. 2차 댈파이 질문지는 1차 델파이 조사를 통해 얻은 문제점 54개에 대해 전문계 고등학교의 입장과 특성화 고등학교의 입장 두 가지 측면에서 문제의 심각성을 체크하도록 하였고 추가적인 의견도 들었다. 3차 텔파이 조사는 2차 델파이 조사 결과 얻은 33개의 문제점에 대해 사분위수를 제시하고 심각성을 체크하도록 하였다. 이 때 사분 위수를 벗어나게 되면 그 이유를 적고,추가적인 의견도 받았다. 그 결과 29개의 문제점이 도출되었으며 그 중 심각성이 높은 13개의 문제점은 학생,교사,교육과정,인식 둥에 관한 것은 아래와 같다. 학생과 관련한 문제점: ① 학생들의 대부분이 진학을 희망한다. ② 전반적인 학생의 가정환경 열악하다. ③ 우수한 학생을 확보하기 어렵다. 교사와 관련한 문제점: ① 교사의 행정업무가 과다하다. ② 우수한 특성화 분야 교원을 확보하기 어렵다. ③ 전공교과 교사의 수업시수가 과다하다. 교육과정과 관련한 문제점: ① 학생의 다양성을 충족할 수 있는 특별교육과정 운영을 위한 예산이 부족하다 ② 특성화 프로그램 벤치마킹 대상이 부족하다. 인식과 관련한 문제점: ① 직업교육에 대한 사회 전반적인 인식이 부족하다. ② 특성화 교육에 대한 학부모의 인식이 부족하다. ③ 특성화 교육에 대한 중학교 교사의 인식이 부족하다 기타 문제점: ① 지속적인 지원이 미흡하다. ② 기초기능 함양의 전문 프로그램 제공처가 부족하다. The purpose of this study was to identify the major problems that teachers perceived in implementing specialized vocational high school education, Specialized vocational high schools are promoted as the main national policy to improve vocational education and strongly supported by the Ministry of Education and Human Resources Development. The Delphi technique for obtaining group consensus was employed to achieve the objective of this study. A panel was composed of 18 head teachers as expert participants selected purposively from 22 specialized vocational high schools which were established over 5 years after transferring as a specialized system. Each panel participant received questionnaires to fill-up through e-mail and responded to it. Three sets of questionnaires were prepared by the researchers. The first questionnaire was an open style to express freely each one's opinion on problems related to specialized vocational education. The second and the third questionnaires composed of questions on scale using the Likert type. Several problems were identified, Problems related to specialized vocational high school students were as follows: ① Most of the students want to go to college/university instead of entering the workplace, ② Generally, they are from a socioeconomically disadvantaged background. ③ It is difficult to attract talented students, which has been a chronic problem for vocational high schools, Problems related to teachers were as follows: ① There are excessive clerical work for teachers ② It is difficult to recruit qualified and competent teachers for the specialized areas ③ Teachers are over-burdened with teaching too many hours of vocational classes, Problems related to the curriculum were as follows: ① There are few programs to benchmark specialized vocational education programs and ② There are no systematic assistance to improve basic academic skills of the students, Problems related to recognition were the following: ① Specialized vocational education carries an unfavorable image, ② There is a lack of parents' recognition of the specialized vocational education and ③ Middle school teachers do not have sufficient information or hold unfavorable perception of specialized vocational schools. Other problems identified include ① There are inadequate financial resources to operate the specialized education programs particularly in coping with student's diversified needs and ② There is a lack of continuous governmental support for the specialized vocational education programs.