야간주차정책에 관한 연구

김광식,박훈립 국민대학교 생산기술연구소 2002 공학기술논문집 Vol.25 No.-

Vehicles must be parked at least temporarily at each end of a vehicular trip before the occupants can partake in their activity. Parking is therefore a fundamental component of urban transportation system. The parking problems however have been got from bad to worse due to high cost of parking improvement. The city governments have formulated the synthetic policies to solve the parking problems without considering the characteristics of night-parking. In this study the direction of the parking policies is presented.

스테로이드 주사를 이용한 이개혈종의 치료

박재율,신승헌,김광훈,이웅렬,박일동,박광헌,마도훈,전일훈 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.2

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

유해화학물질의 계통분석

박성우,박유신,이진훈,김남이,김유나,임미애,이상기,유재훈,진광호,신호상,노동석 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

연폭로 수준에 의한 공기중 연농도와 혈액중 연농도간의 상관성

박석건,김광종,장성훈 大韓産業醫學會 1991 대한직업환경의학회지 Vol.3 No.1

The correlation between the lead concentration in air at work site and in blood among three different groups of workers was studied. The test subjects were 50 lead solderers in an eleotronic company(Group 1). 66 lead smelters(Group 2). 40 workers in storage battery factory(Group 3). The results were as follows: 1. The geometric mean of air lead concentration in Group 3 was the highest level among the three groups at 0.226 mg/㎥ (0.070-1.475 mg/㎥). The lead concentrations at work sites were found to be significantly different among, the three groups. 2. The mean of blood of lead concentration in Group 3 was 74.3 ㎍/dl(17.0-149.3 ㎍/dl) was also the highest level among the three groups. The levels of lead concentration among the three groups were significantly different. 3. In Group 2 and 3, the correlation coefficient between lead concentration in air and in blood was statistically significant. The correlation coeffioient was found to increase with the level of lead exposure.

Human Glutathione S-transferase의 Tyr108 잔기의 역할에 관한 연구

박희중,공광훈 中央大學校 遺傳工學硏究所 1999 遺傳工學硏究論集 Vol.12 No.1

Glutathione S-transferase(GST, EC 2.5. 1.18)의 기질 결합부위에 대한 연구를 위하여 human GST P 1-1의 친전자성 기질 결합부위 (H-site)로 알려지고 있는 Tyr108 잔기에 대하여 부위특이적 변이법을 이용하여 Y108A, Y108F 그리고 Y108W의 새로운 변이체 3개를 얻었다. 대장균을 이용한 대량발현과 affinity column에 의해 정제된 변이체 효소에 대하여 GSH와 CDNB, DCNB, ETA, EPNP 또는 steroid와의 포합반응 활성을 측정하여 야생형과의 활성비교를 하였다. 야생형과의 활성비교에 있어서 각 변이체들의 변화를 볼 수 있었으며, 특히 입체적 장애를 준 Y108W 변이체의 경우 CDNB에 대한 활성이 다른 변이체에 비해 현저히 감소한 반면 ETA에 대한 활성은 증가하였다. 이러한 결과 Tyr108 잔기는 친전자성 기질 결합부위에 관련되는 잔기로 생각되어진다. In order to study the role of residue in the active site of glutathione S-transferase (GST), Tyr108 residue in human GST P 1-1 was replaced with alanine, phenylalanine and tryptophan by site-directed mutagenesis to obtain mutants Y108A, Y108F and Y108W. The specific activities were determined by measuring the initial rates of the enzymes-catalyzed conjugation of GSH with CDNB, DCNB, ETA, EPNP or steroid. The Y108W mutant slightly increased the conjugating activity toward ethacrynic acid(ETA)., but it decreased in specific activity toward 1-chloro-2,4-nitrobenzene(CDNB). On the other hand, the Y108A and Y108F mutants had negligible effect on the activity toward CDNB, ETA and DCNB of the wild type. These results indicated that Tyr108 in human GST P1-1 may contribute to the binding of electrophilic substrate.

Human tyrosinase 유전자의 대장균에서의 발현

박소영,공광훈,박희중 中央大學校 遺傳工學硏究所 1997 遺傳工學硏究論集 Vol.10 No.1

Tyrosinase는 활성부위에 한 쌍의 구리이온을 함유하고 있는 metalloprotein으로 멜라닌 생합성에 중요한 역할을 한다. Human tyrosinase의 메카니즘을 연구하기 위해 human tyrosinase 유전자에 대한 대장균에서의 대량발현계를 구축하였다. Plasmid pRHOHT2 (Shibahara et al, 1988. Tohoku J. Exp. Med. 156. 403)로부터 human tyrosinase cDNA sequence는 polymerase chain reaction에 의해 증폭되었으며, 발현벡터 pGEX-KG와 pTrcHis에 subcloning 되었다. 이렇게 작성된 plasmid pKG-Tyrosinase와 pHis-Tyrosinase를 대장균주 MV1190에 형질전환시켜, 유도제 isopropyl- β-D-thiogalactopyranoside로 human tyrosinase를 대량발현시켰다. 발현된 tyrosinase는 GSH affinity column과 immobilized metal affinity column으로 정제하였고, 정제된 재조합 tyrosinase는 SDS-gel 전기영동상에서 각각 84,000과 66,000 정도를 나타내었다. 또한 정제된 재조합 tyrosinase는 dopa oxidation 활성을 나타내었다. Tyrosinase is a bifunctional copper-containing metalloprotein and play a central role and of melanin biosynthesis. In the study, we constructed several E. coil-expression system of human tyrosinase using a cDNA encoding human tyrosinase for its mechanism study. Human tyrosinase cDNA sequence, plasmid pRHOHT2 (Shibahara et al. 1988, Tohoku J. Exp. Med. 156, 403), was amplified by the polymerase chain reaction and was subcloned with pGEX-KG and pTrcHis expression vector. These recombinant tyrosinases were produced by induction with isopropyl-β-D-thiogalactopyranoside. Overexpressed human tyrosinases were purified by GSH affinity column and immobilized metal affinity column. The molecular weights of the purified recombinant tyrosinases determined by SDS-polyacrylamide gel electrophoresis was about 84,000 (pKG-Tyrosinase) and 66,000 (pHis-Tyrosinase), respectively. These enzymes also showed dopa oxidation activity.

GIS에 의한 의사결정 시스템 구축에서 AHP의 적용기법

박재훈,양인태,최광식 江原大學校 産業技術硏究所 1998 産業技術硏究 Vol.18 No.-

According to growing interest in environmental quality with improvements of a standard of livign site selection problems such as waste landfill sites, caused regional conflicts. This study investigates a waste landfill estimation method that was used to for storage, management, analysis and dispaly of encironmental information provided by geo-spatial information system(GLS) and analytic hieracchy process (AHP) as a decision-making method. If GLS is integrated with AHP, site selection problems of environmental hatred facilities shall be able to be very useful, because of AHP with flexibility which appropriately reflects opinions of the related group.

Capillary Zone Electrophoresis를 이용한 음이온 동시분석에 관한 연구

박성우,진광호,유재훈,김동환 內務部 國立科學搜査硏究所 1996 國立 科學 搜査 硏究所 年報 Vol.28 No.-

포도 뿌리혹병 방제를 위한 길항균 선발

박광훈,김정희,차재순 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 2002 연구보고서 Vol.6 No.-

Previous study showed that crown gall disease is very severe on the tetraploid cultivars of grape. One of the promising control measures of the disease is biological control using a antagonist against pathogen, Agrobacterium vitis. Total 215 bacteria and 21 putative agrobacteria were isolated from galls of grapevine and a Korean wild grape, Vitis amurensis respectively. Antagonistic ability of the isolates was checked by growth inhibition test of pathogens, A. tumefaciens and A. vitis on agar and on tamato seedling by co-inoculation of the isolates and pathogen. Two selected antagonists, isolate 197 and Va 21 strongly suppressed gall formation of A. vitis K306 on grapevine when they were co-inoculated with pathogen K306. Both gall size and rate of gall formation were significantly lower on grapevines inoculated of 197-K306(9:1) or Va 21-K306(9:1) mixture than on them inoculated of just K306. Antagonistic ability of the two isolates was similar to F2/5, which is known as a strongest antagonist against A. vitis so far. Further field study for biological control of crown gall disease on grapevine by the two isolates is needed.