Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

他科醫師의 精神科 諮問에 대한 期待度

韓辰熙,柳太烈,李秀晶 大韓神經精神醫學會 1991 신경정신의학 Vol.30 No.1

To investigate physicians' expectations to the role of psychiatric consultation, the authors conducted 18-item questionnaire on 117 nonpsychiatric physicians of two university affiliated general hospitals(St. Mary's Hospital and Kang Nam St. Mary's Hospital) in Seoul. The results were as follows : 1) Physicians rated, in general, high scores on items related with `evaluation function ' and `direct service function' of psychiatric consultation, whereas they rated low on `mediating function' items. 2) `Evaluate mental status', `Help physicians understand psychological aspects of a patient's illness', ` Arrange to transfer patient to psychiatric ward', `Evaluate psychogenesis' and `Make suggestions about management of patient on the ward' are those which phisicians valued most important of all 18 items. 3) There was a tendency that resident group manifested low item scores compared to faculty members, and items of `Arrange to transfer patient to psychiatric ward' and `Recommand additional diagnostic work-up' showed significant difference in mean scores between groups. 4) Physicians in medical services showed significant high mean scores on items such as `Arrange to transfer patient to psychiatric ward', `Make suggestions about management of patient on the ward', `Evaluate psychogenesis' and `Work with patient's family' as compared to surgeon group.

小豆의 交酸集團에 따른 世代別 遺傳子 分布狀態 및 組合能力의 檢定

韓鏡秀,張權烈,金鎭馨 慶尙大學校 1985 論文集 Vol.24 No.1

The two sets of diallel crosses (5×5 and 9×9 crosses) from nine adzukibean varieties (Yeongdong Jeogdu, Gongju Baegdu, Gyeonggi Gaeguri, Wase Dairuyu No. 1, Natsu Adzuki, Maruba No. 1, Jeogdu, Nesumi Mochi and Hongcheon Jeogdu) were grown in the field for estimating genetic parameters, such as gene action and combining ability in two different generations, F_1 and F_2. From a series of experiments conducted in 1976 to 1977, 1981 to 1982, and two agronomic characters, i.e., days to flowering and 100-grain weight were observed. The results obtained are summarized as follows: 1, In finks and Hayman's (Vr-Wr) graphic analyses, days to flowering and 100-grain weight of F_1's and F_2's from 5×5 and 9×9 crosses were found to be inherited in partial dominance. 2. Mean squares of GCA and SCA were significantly different among two characters in all generations of different crosses, and those of GCA were higher than SCA. 3. The highest GCA effects for days to flowering were expressed Jeogdu in 5×5 cross, Hongcheon Jeogdu in 9×9 cross, ana Jeogdu for 100-grain weight showed relatively high effects in two generations of 5×5 and 9×9 crosses. 4. SCA effects were different among Parents and characters in the different crosses, but the higher SCA effects were exhibited in the hybrid between Yeongdong Jeogdu and Nesumi Mochi in two generations of 5×5 cross.

상완골 간부 분쇄 골절의 최소 절개를 통한 관혈적 정복 및 교합성 골수강내 금속정 고정술 : 금속판 내고정술을 이용한 상완골 간부 분쇄 골절 치료와의 비교 Comparison between Plate Internal Fixation

한경진,어수익,김태영,강신영 대한골절학회 2002 대한골절학회지 Vol.15 No.4

목 적 : 상완골 간부 분쇄 골절의 치료에서 최소 절개를 통한 관혈적 정복 및 교합성 골수강내 금속정 고정술의 치료 결과를 금속판 내고정술과 비교하고자 한다. 대상 및 방법 : 1997년 3월부터 2001년 12월까지 상완골 간부 골절로 금속판 내고정술을 시행한 14례와 상완골 간부 분쇄골절에 대해 최소 절개를 통한 관혈적 정복 및 교합성 골수강내 금속정 고정술을 이용하여 수술을 시행한 18례이고, 최고 9개월 이상 추시하였으며 임상적 및 방사선학적 결과를 분석하였다. American Shoulder and Elbow Surgeon's (ASES) shoulder score를 기준으로 기능적 평가를 하였고, visual analous scales에 따라 통증의 정도 (zero : no pain ~ 10 : extreme pain) 를 평가하였다. 결 과 : 골절 유합은, 금속판 내고정술의 경우 14례중 12례(85.7%)에서 골유합을 얻었고 골유합 시기는 평균 13.2주 이었다. 최소 절개를 통한 관혈적 정복 및 교합성 골수강내 금속정 고정술을 이용하여 수술을 시행한 18례중 17례(94.4%)에서 골유합을 얻었고 평균 골유합 시기는 평균 12.4주 이었다. ASES shoulder score에 따른 기능적 점수는 금속판 내고정술의 경우 평균 44점, 최소 절개를 통한 관혈적 정복 및 교합성 골수강내 금속정 고정술을 시행한 경우 평균 47점 이었다. 통증의 정도는 금속판 내고정술의 경우 평균 1.8, 최소 절개를 통한 관혈적 정복 및 교합성 골수강내 금속정 고정술을 시행한 경우 평균 1.4 이었다. 결 론 : 상완골 간부 분쇄골절에 대한 최소 절개를 통한 관혈적 정복 및 교합성 골수강내 금속정 고정술은 견고하고 정확한 골절의 정복이 가능하고 술후 방사선학적 및 기능적 결과가 다른 수술방법에 비해 결과가 좋은 것으로 사료되었다. Purpose : We compared the functional and radiological results after the minimal open reduction and interlocking IM nailing and LC-DCP plate internal fixation for the comminuted humeral shaft fractures. Materials and Methods : Fourteen plates(LC-DCP) and eighteen interlocking IM nail (AO unreamed IM nail) were applied after open reduction for 21 comminuted fractures of the humeral shaft between March 1997 and December 2001. They were followed up for a minimum 9 months after surgery and the radiological and functional results were evaluated. Results : The average fracture healing time was 13.2 weeks and union rate was 85.7% for plate internal fixation. The average fracture healing time was 12.4 weeks and union rate was 94,4% for interlocking IM nail. The average functional scores according to American Shoulder and Elbow Surgeon's (ASES) shoulder score(Total 52 points_ was 44 points for plate internal fixation and 47 point for interlocking IM nailing respectively. Conclusion : Minimal open reduction and interlocking IM nailing is better method with good functional and radiological results than plate internal fixation for the comminuted humeral shaft fractures.

전위된 소아 상완골 과상부 골절의 치료 : 도수정복 되지 않는 골절의 최소 절개를 통한 정복술 Open Reduction with Minimal Incision of the Manually Irreducible Fracture

한경진,어수익,박응석,강신영 대한골절학회 2002 대한골절학회지 Vol.15 No.4

목 적 : 전위된 소아 상완골 과상부 골절의 치료에서 도수 정복 후 석고 고정술 또는 경피적 K-강선 고정술을 시행하거나 도수 정복이 되지 않은 경우 최소 피부절개를 통한 관혈적 정복 및 경피적 K-강선 고정술을 시행한 결과를 분석하고자 하였다. 대상 및 방법 : 전위된 상완골 과상부 골절(Gartland 제 Ⅱ, Ⅲ형)로 치료한 8세 이하의 환아중 1년 이상 추시가 되었던 총 74명 (74 골절) 을 대상으로 하였다. Gartland분류에 의한 제 Ⅱ형이 19차례, 제Ⅲ형이 52차례 이었고 정복의 기준은 건측의 주관절 Bauman 각으로 하였다. 안정적인Gartland 제 Ⅱ형, 6례에서 석고 고정을 시행하였고 불안정한 Gartland 제 Ⅱ형 13례와 Gartland 제Ⅲ형 52 례는 경피적 K-강선 고정술을 시행하였다. 도수 정복이 되지 않은 20례 (제 Ⅱ 형 1례, 제 Ⅲ 형 19례)의 경우, mini C-arm 영상하에 주관절 후면에 최소절개를 가하고 소절골도를 이용하여 정복하였다. 결과의 분석에는 주관절 운반각과 기능적인 운동범위의 소실 정도에 따른 Flynn의 분류를 사용하였다. 결과 : 저자들의 결과를Flynn의 분류에 따라 분석하면, 운반각의 경우 excellent는 52 례 (73.2%). good은 13례(18.3%) 로 만족할 만한 결과는 91.5% 이고 운동 범위의 경우 excellent는 49례 (69.0%), good은 20례(28.2%) 로 만족할 만한 결과는 97.2% 로서 비슷한 결과를 보였다. 도수 정복 및 경피적 K-강선 고정술이 운동 범위 요소(Functional factor)에서 더 많은 excellent, 그리고 최소 절개를 이용한 관혈적 정복 및 경피적 K-강선 고정술은 운반각 요소(Cosmetic factor)에서 더 많은 excellent를 나타내었다. 결 론 : 소아의 상완골 과상부 골절의 치료는 소아 골단의 성장판의 손상과 향후 발생할 수 있는 각변형을 고려하여 되도록 정확하고 세심한 정복과 안정적인 고정을 요한다. 또한, 도수 정복이 불가능할 경우 무리한 도수 정복 조작을 계속하지 말고 관혈적 정복을 시행하며 연부조직 손상을 줄이기 위해 가능한 최소 절개를 통한 정복 및 경피적 K-강선 고정을 하는 것이 좋은 결과를 나타내는 것으로 생각된다. Purpose : We evaluated the results of the displaced supracondylar fractures in children treated by closed reduction and cast immobilization, closed reduction and crossed K-wires fixation. Materials and Method : We retrospectively reviewed the results after 1 years of the treatment of 71 extension-type fractures (Gartland type Ⅱ-19 cases, type Ⅲ-52 cases) of supracondylar humeral fractures in children under age of 8 years. Closed reduction was impossible in 20 cases (1 type Ⅱ, 19 type Ⅲ) and we performed open reduction with minimal incision and cross percutaneous pinning. We measured Baumanns angle and range of motion of elbow and evaluated the results by Flynns criteria. Result : By Flynns criteria, 91.5 % of satisfactory (excellent, good) resuts in cosmetic factor and 97.2 % in more excellent in functional factor and that of the minimal open reduction and percutaneous pinning in cosmetic factor but over all satisfactory results were similar. Conclusion : The treatment of the displaced supracondylar humeral fractures in children need accurate and delicate reduction and firm fixation to prevent deformity and to preserve function of the elbow. Open reduction with minimal incision instead of general incision for the manually irreducible supracondylar humeral fracture would be a reliable and convenient method for the treatment of supracondylar humeral fractures in children.

철봉 어깨 틀어 휘돌며 다리 모아 엔도(Endo) 동작의 운동학적 분석

한윤수,백진호,이용식 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

This study was attempted to kinematical characteristics of the Endo El-grip Swing on the horizontal bar. At 2002 Busan Asian Games, three endo piked in el-grip swing were filmed using two video cameras during men's team competition. The three dimensional motion analysis with DLT method was executed using two video cameras of analyzing the actual competition situation. It was summarized that (a) It was indicated large hip and shoulder angle at E1, small hip angle and large sholder angle from E1 to E2, the smallest hip angle at E3, flexional hip and shoulder angle from E3 to E4, large hip and shoulder angle at E5; (b) It was indicated large angular velocity of hip at E3, large angular velocity of trunk rotation from E2 to E4, small angular velocity of shoulder and trunk rotion.

小豆 成分育種을 위한 主要化學成分의 遺傳分析

韓鏡秀,張權烈,金鎭馨 慶尙大學校 1985 論文集 Vol.24 No.2

小豆 成分育種올 위해 主要化學成分의 遺傳關係를 早期에 推定하고 앞으로의 育種計劃에 대한 基礎資料를 얻고자, F_l, F_2 兩世代의 優性程度 및 遺傳子 分布狀態, 그리고 遺傳力과 相關係數等을 推定한 바, 그 結果는 다음과 같다. 1. 優性程度는 全成分에서 各各 超優性으로 遺傳되었으나 Mg는 不完全優性(F_l世代)과 超優性(F_2世代)으로 世代間에 差異가 있었고, F_l 世代의 粗脂肪, 灰分및 Mg와, F_2 世代의 灰分에서는 非對立遺傳子의 關與가 多少 있었다. 2. 各 成分의 遺傳力은 兩世代間에 비교적 낮았으나 K와 粗脂肪은 他成分보다 높았다. 3. 形質相互間의 相關關係에서 灰水化物은 他成分間과 負의 相關을 보였고, 기타 成分들은 相互 正의 相關이었는데, 蛋白質과 粗脂肪은 F_2世代에서 有意한 正의 相關을 나타내었다. A set of 36 crosses of F/s and F_2's from 9 X9 diallel crosses of nine azdukibean varieties were analyzed for estimating genetic parameters, such as gene action, degree of dominance and genotypic correlation. Seven chemical com positions, i. e., carbohydrate, protein, crude fat, ash, K, Ca and Mg were observed. The results obtained are summarized as follows: 1. In Vr-Wr graphical analyses, all chemical compositions except Mg in F/s were found to be inherited in over dominance. 2. The highest heritability value was found in K in both F/s and F/s, but the value of ash was the lowest. 3. The genotypic correlations between carbohydrate and other chemical compositions were negatively correlated, but that between protein and crude fat was positively correlated in F_2's.

서울시 재건축에 미친 관련 법령의 영향요인 분석

한수진,박신영,윤영호 대한건축학회 2004 대한건축학회논문집 Vol.20 No.6

The aim of this study is to prove that reconstruction policy has more effect on reconstruction projects than market by analysing the characteristics of reconstruced projects in Seoul from 1987 to 2002. The average of the expected life span of reconstructed housing is only 20.5 year. 296,637 housing unit was newly constructed where 163,485 housing unit was demolished in the case of Seoul. According to the change of reconstruction policy of housing, the number of reconstruction union and cases has been changed, The less regulated, the number of them increased while the more regulated, the number of them decreased.