추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 1종의 Sterol과 3종의 배당체 화합물의 단리·동정

이유건(Yu Geon Lee1),조정용(Jeong-Yong Cho),이현주(Hyun Joo Lee),이용현(Yong Hyun Lee),이상현(Sang-Hyun Lee),한태호(Tae-Ho Han),김월수(Wol-Soo Kim),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.5

본 연구에서는 배의 유용성 증명을 위한 일환으로 배의 화학성분을 분자수준에서 밝히고자 하였다. 이에 배 과피 MeOH 추출물을 용매분획하여 얻은 EtOAc-산성 분획과 EtOAc-중성분획을 대상으로 Sephadex LH-20, silica gel, 그리고 ODS colmn chromatography와 HPLC를 이용하여 정제 및 단리하였다. 그 결과, EtOAc-산성 분획과 EtOAc-중성 분획으로부터 각각 2종씩의 화합물을 단리하였다. 단리된 화합물 1-4는 1H- 및 13C-NMR 분석을 통하여 각각 (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (화합물 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone(piceoside, 화합물 2), β-sitosterol (화합물 3), 그리고 β-sitosteryl 3-O-β-D-glucopyranoside (화합물 4)로 동정되었다. 단리된 3종의 배당체 화합물(화합물 1, 2, 4)들은 본 연구에 의해 배로부터 처음 동정되었으며, 화합물 3은 추황배로부터 처음 동정되었다. 본 연구결과가 배 함유 성분연구는 물론 배의 기능성 해명 연구에도 추후 중요한 기초자료로 활용되길 기대한다. We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (compound 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), β-sitosterol (compound 3), and β-sitosteryl 3-O-β-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.

정상인에서 부분적 수면박탈이 수면구조에 미치는 영향

왕성근,이정규,이충숙,최하석,유남재,정인형,이선우 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

To identify the effects of partial sleep deprivation on the sleep structure, the authors performed polysomnographic study for 9 healthy young adults. The subjects were college students, ranging in age from 20 to 25 years, without personal past or present histories of medical, neurological, or psychiatric illness and suffering from current sleep disturbance or parasomomnias. Sleep records were analyzed according to the criteria of Rechtschaffen and Kales' manual and tried paired t-test. The results were summarized as follows. 1. As for sleep parameters, total sleep time(TST), REM latency and REM duration were not changed significantly during deprivation period and recovery period compared with baseline. Sleep efficiency increased significantly during deprivation period and recovery period compaired with baseline, and sleep latency and WASO were shortened significantly during deprivation period and recovery period compared with baseline. 2. As for sleep structure, 1) The absolute value(minutes) of stage 1 decreased significantly during deprivation period and first recovery night compared with baseline, and the relative value(percentage) of stage 1 decreased significantly during deprivation period and recovery period compared with baseline. 2) The absolute value(minutes) of stage 2 decreased significantly during deprivation period compared with baseline, and the relative value(percentage) of stage 2 decreased significantly during deprivation period and first recovery night compared with baseline. 3) The absolute value(minutes) of slow wave sleep were not changed significantly during deprivation period and recovery period compared with baseline, and the relative value(percentage) of slow wave sleep increased significantly during recovery period compared with baseline. 4) The absolute value(minutes) of REM sleep decreased significantly during deprivation period compared with baseline, and the relative value(percentage) of REM sleep decreased significantly in the first deprivation night but, were not changed significantly during recovery period compared with baseline.

관상동맥 질환에서 SDF-1α의 농도

김보영,박용규,박형서,노상필,정승현,이유선,이정우,이재환,최시완,정진옥,성인환 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

Atherosclerosis is now viewed as an inflammatory disease of the vascular system. Expression of several chemokines, including monocyte chemoattractant protein(MCP)-1, MCP-4, RANTES(regulated on activation normal T-cell expressed and secreted), and interleukin-8(IL-8) are increased in human atherosclerotic plaques compared with normal vessels. They are involved in the pathogenesis of atherosclerosis and plaque rupture by activating and directing leukocytes into the atherosclerotic lesions. However, some are involved in homeostatic functions such as normal leukocyte traffic and growth regulation. SDF-la is a multi-functional cytokine that is involved in myelogenesis, hematopoiesis, angiogenesis and injured gastric mucosal regeneration in the gastric ulcer patient. SDF-la is recently shown to be highly expressed in atherosclerotic plaques and a potent platelet agonist. At least in high concentrations, SDF-la may mediate antiinflammatory and matrix stabilizing effects in unstable angina. Many studies are going on to know the function of SDF-la in coronary artery diseases. I investigated the difference of the plasma level of SDF-la between control group and coronary artery disease group. Total 75 subjects were enrolled. The diagnosis of coronary artery disease was confirmed in all patients by coronary angiography. Control subjects in this study were confirmed normal by coronary angiography. Clinical profile and risk factors were also reviewed. Control subjects in this study were 27 (M=10, F=17). Plasma for the study was collected before the angiography and centrifuged. SDF-la analysis was performed by ELISA. Plasma level of SDF-la is significantly increased in patients with stable angina(n=20) and unstable angina group(n=28) compared with healthy control group(n=27). the risk factors do not influence the plasma level of SDF-la in coronary artery diseases. In this study, plasma level of SDF-la is increased in patients with stable angina and unstable angina groups compared with healthy control group(P<0.05). the risk factors do not influence the plasma level of SDF-la in coronary artery diseases.

유치원아동에서 고자당배지를 이용한 우식활성검사치와 우식경험유치면지수와의 상관성

이광희,김유정 大韓小兒齒科學會 1991 大韓小兒齒科學會誌 Vol.18 No.2

116 preschool-children were examined for caries activity and dmfs index.20% Sucrose-sulphasomidine-broth was used to cultivate plaque sample that was obtained from buccal side of maxillary posterior teeth. After incubation for 48 hours and 72 hours, acid production of Streptococcus mutans was evaluated by the color change of pH indicator(5% Bromcresol green) in the medium. 1.There was significant correlation between dmfs index and caries activity test scores(P<0.05). 2.Age and sex did not significantly affect caries activity test scores. 3.By cultivating plaque sample in 20% sucrose-sulphasomidine-broth over 72 hours caries-inactive children can be differentiated from caries-active children.

충수돌기에 발생한 비호지킨 림프종 1예

정유경,이미숙,김윤식,이미자,전호종 조선의대 1995 The Medical Journal of Chosun University Vol.20 No.2

신이식 환자에서 자발적으로 발생한 저구연산뇨증 연관성 요관 결석로

나정구,공성민,이동규,김성민,장윤정,김성록,이유지 이화여자대학교 의과학연구소 2015 EMJ (Ewha medical journal) Vol.38 No.3

Urolithiasis is an uncommon complication in renal transplantation. We report a case of hypocitraturia-related ureteral steinstrasse which was spontaneously formed in a renal transplant recipient. The patient who underwent renal transplantation was admitted with acute pyelonephritis. Hydronephrosis in the transplanted kidney and multiple stones (steinstrasse) in the distal ureter were incidentally found on computed tomography scanning. After a failed attempt of ureteroscopic removal of stones, the patient underwent open ureterolithotomy and ureteroureterostomy. On stone analysis, carbonate apatite was confirmed. Urinary citric acid levels were decreased to 127.6 mg/day. Potassium citrate was administered to prevent stone recurrence by increasing urinary citrate excretion. No recurrence of stones was shown six months later. Urolithiasis in renal transplant recipients requires a high index of suspicion. Hypocitraturia can increase the risk for urolithiasis. Rapid recognition by careful surveillance, prompt removal of stones, and precautionary efforts to prevent recurrence are needed.

환경경영시스템의 효과 제고를 위한 환경측면 파악방법 연구

정유심,이상용,김회배 한국품질경영학회 2000 품질혁신 Vol.1 No.1

96년 ISO 14001 국제규격의 개발과 동시에 우리나라에서도 본격적인 환경경영시스템 인증제도가 시작되어, 기업경영에서 환경문제에 대한 적극적인 고려가 가시화되기 시작했다. 한편, 최근 사회적으로 환경에 대한 인식이 증가하면서 환경경영시스템 도입을 통해 이러한 인식에 부응하여 다양한 경영성과를 기대하는 조직의 요구도 커지고 있는 상황이다. 조직의 관점에서 경영성과란 결국 조직의 수익창출에 기여할 수 있는 성과를 거두는 것이기 때문에 환경경영시스템이 추구하는 목적은 환경영향의 저감을 통해 이러한 효과를 달성코자 하는 것이다. 이와 관련하여 본 연구에서는 조직이 발생시키는 환경영향의 요인, 즉 환경측면과 그 파악방법에 대한 이해를 통해 환경경영시스템의 효과 제고를 모색코자 한다.

방위에 따른 속리산과 오대산의 산림군락 구조분석

유재은,이정호,권기원 명지대학교 자연과학연구소 2004 자연과학논문집 Vol.23 No.-

본 연구는 방위에 따른 속리산 및 오대산 삼림식생을 분석하고자 하였다. 속리산 조사구와 오대산 조사구를 방위에 따라 출현하는 주요 종의 중요치를 살펴보면 속리산의 경우 북향에서는 쇠물푸레, 벚나무, 개옻나무, 굴피나무, 참회나무 등 8종에 중요치가 높게 분포하고 있음을 볼 수 있고, 동향에서는 소나무, 고로쇠나무, 산초나무, 소태나무, 작살나무 등 6종이, 남향에서는 굴참나무, 졸참나무, 생강나무, 물푸레나무, 짝자래나무 등 13종이, 서향에서는 신갈나무, 당단풍, 쪽동백나무, 노린재나무, 철쭉 등 13종에 중요치가 높게 출현한다. 오대산의 경우를 보면 북향에서는 당단풍, 까치박달, 거제수나무, 음나무, 잣나무등 18종이 중요치가 높게 분포하고 있음을 볼 수 있고, 동향에서는 전나무, 소나무, 느릅나무, 산벚나무, 들메나무 등 5종이, 남향에서는 신간나무, 물푸레나무, 철쭉, 진달래, 개벚지나무 등 7종이, 서향에서는 피나무, 고로쇠나무, 난티나무, 쪽동백나무, 황벽나무 등 10종이 중요치가 높게 출현함을 알 수 있다. 이러한 결과로 볼 때 속리산에서는 남향과 서향이 각각 13종씩 26종으로 북향과 동향의 14종 보다 배에 가까운 많은 수의 나무들이 중요치가 높게 출현하고 있는 것에 반하여, 오대산에서는 북향과 동향이 각각 18종과 5종씩 23종으로 남향과 서향의 17종 보다 월등히 많게 출현함을 알 수 있다. 이와 같은 결과는 속리산과 오대산이 정반대의 현상으로 속리산이 해발고가 낮고 난온대성 수종의 우위를 점하는 것에 비하여 오대산은 해발고가 높고 냉온대성 수종이 우위를 차지하고 있는데서 오는 현상으로 보여진다. This study was to research environment of the forest vegetation structure according to the directions in Mt. Sokri and Odae. According to direction, the importance value of 8 species of Fraxinus sieboldiana, Prunus serrulate var. spontanea, Rhus trichocarpa ect. appeared highly on the north, 6 species of Pinus densiflora, Acer mono, Zanthoxylum schinifolium ect. highly appeared on the east, 13 species of Quercus variabilis, Quercus serrata, Lindera obtusiloba ect. highly appeared on the south, 13 species of Quercus mongolica, Acer pseudo-sieboldianum, styrax obassia ect. highly appeared appreared on the west in Mt. Sokri. The importance value of 18 species of Acer pseudo-sieboldianum, Carpinus cordata, Betula costata ect. highly appeared on the north, 5 species of Abies holophylla, Pinus densiflora, Ulmus dabidiana var. japonica ect. highly appeared on the east, 7 species of Quercus on the south, 10 species of Tilia amurensis, Acer mono, Ulmus laciniata ect. highly appeared on the west in Mt. Odae

외상 환자에서 의식소실 유무와 외상후 스트레스 장애의 유병율, 증상 양상, 우울 및 불안에 관한 연구

이유진,연규월,우행원,김영철,임원정 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.4

연구목적 : 본 연구에서는 의식 소실 유무에 따른 외상후 스트레스 장애 유병율 및 증상, 우울, 불안 증상을 알아보기 위해 외상을 당한 환자를 의식 소실이 있었던 환자군과 의식 소실이 없었던 환자군으로 구분하여 두 집단의 외상후 스트레스 장애의 유병율, 증상 및 불안 정도를 비교해보고자 하였다. 방 법 : 사고를 당하고 1달 이상 경과한 환자 120명(남자 69명, 여자 51명)을 대상으로 하였다. 대상군을 의식 소실 유무에 따라 소실군과 비소실군으로 구분하고 사회인구학적 변수, 사고 당시 의식 소실의 유무, 의식 소실 시간, 사고의 종류, 사고에 대한 기억, 사고의 심각도, 손상 심각도 점수(Injury Severity Score)를 조사하여비교하였다. 또한 외상후 스트레스 장애 척도(Clinician Adminstered PTSD Scale), Beck의 우울증 척도(Beck Depression Inventory), 상태 특성 불안처도- Ⅰ, Ⅱ(State Trait Anxiety Inventory- Ⅰ, Ⅱ)를 이용하여 조사하여 두 군 사이의 외상후 스트레스 장애유병율 및 증상, 우울, 불안 정도를 비교하였다. 결 과 : 통 연구 대상자 120명중 의식 소실이 있었던 환자군(소실군)은 56명(46.7%)이었고 의식 소실이 없었던 환자군(비소실군)은 64명(53.3%)이었다. 총 대상자 120명중 30명(25%)이 외상후 스트레스 장애로 진단되었으며 소실군 56명중 17명(30.4%)이 비소실군 64명중 13명(20.3%0이 각각 외상후 스트레스 장애로 진단되었으나 두 군간의 차이는 통계적으로 유의하지 않았다(x^2=1.607, P>0.05). 외상후 스트레스 장애 증상 척도에서는 소실군에서 사고에 대한 기억 상실, 흥미 상실, 이탈, 감정 둔마, 수면자애, 예민함, 집중력 감소 증상의 빈도가 유의하게 높았다(P<0.05). 비소실군에서느 반복적인 회상과 놀람 반응 빈도가 유의하게 높았다(p<0.05). Beck의 우울증 척도 점수는 소실군에서 38.8±8.8, 비소실군에서 31.0±7.9로 의식 소실을 보고한 환자군의 점수가 유의하게 높았으며(t=-3.16, 0<0.05) 상태 특성 불안 척도-I, II는 소실군에서 각각 44.5±3.3, 44.6±3.9, 비소실군에서 각각 42.9±3.0, 42.3±3.4로 소실군의 점수가 유의하게 높았다(t=-2.75, p<0.05, t=-3.38, p<0.05). 결 론 : 사고 당시 의식 소실이 없었던 환자군과 비교하여 외상후 스트레스장애가 비슷한 빈도로 진단되며 의식 소실이 외상후 스트레스 장애의 발생에 유의한 영향을 미침은 물론 불안 및 우울에도 유의한 영향을 미친다고 할 수 있다. Objectives :This study investigated PTSD prevalence, symptoms, depressed mood and anxiety, comparing two groups of the patients in trauma ; one who had experienced the loss of consciousness(group "A" here after) and the other group of patients who had not(group "B" here after). Method : subjects were 120 patients(age18-66) who had received trauma(traffic accident, fall down) more than 1 month. Before they consisted of 56 patients who had experienced the loss of the consciousness(46.7%) and 64 who had not(53.3%). Men were 69 and women were 51. Clinician administered PTSD Scale, Beck Depression Inventory, State Trait Anxiety Inventory-I, II were administered. The factors such as sociodemographic variables, unconsciousness at the time of trauma, memory about tranmatic accidents were considered. Result : Out of 120 subjects, 30 patients(25%) were diagnosed as PTSD : 17 from 56(30.4%) and 13 from 64(20.3%) were found in the group "A" and "B" respectively. Prevalence of PTSD was higher in the group "A" showed significant difference between two groups(x^2=1.607,p>0.05). The CAPS of group "A" showed significantly high frequency in the loss of interest, detachment, restricted affect, sleep difficulty, irritability/anger. concentration difficulty(p<0.05), while the group "B" scored high in intrusive recall and increased startle. The scores of BDI, STAI-I, II were significantly higher in the group "A"(t=-3.16, p<0.05)(t=-2.75, p<0.05, t=-3.38, p<0.05). Conclusion : PTSD was mre frequent and depressed mood and anxiety appeared more often in the group qho experienced the loss of consciousness. Thus the loss of the consciousness at the time of trauma prones of the aevelopment PTSD and symptom of aepression and anxiety.

골뱅이(Buccinum Undatum) 알레르기 : 감작률과 다른 음식물 항원과의 연관성 Sensitization rate and its relationship with other food allergens

이재영,윤성호,서유진,최정희,서창희,남동호,김윤근,민경업,박해심 대한천식 및 알레르기학회 2004 천식 및 알레르기 Vol.24 No.1

Background : The common whelk (Buccinum undatum) is one of the largest and most common snails in the North Atlantic. In Korea and Japan, common whelk is a popular eatable shellfish. Although shellfish has been known as the one of the most common causes of food allergy, there has been no published report on allergenecity and clinical significance of this sea snail. In this study, we determined the sensitization rate to common whelk and its relationship with other food allergens in allergy patients. Method : We carried out the skin prick test (SPT) with commonly consumed food stuffs in Korea including common whelk in 1700 patients over 1 year. The specific IgE to common whelk were detected by enzyme-linked immunosorbent assay (ELISA). ELISA inhibition test using sensitized sera was conducted. Results : SPT to common whelk was positive (≥2+) in 83 (4.9%) patients studied. Twenty-four (38.7%) of 62 SPT-positive patients had high serum specific IgE to common whelk. ELISA inhibition test showed significant inhibitions by abalone as well as by common whelk and, minimal inhibition were noted by shrimp. Significant correlation was also noted in specific IgE levels between common whelk and abalone (r=0.58, F<.05). Conclusion : IgE-sensitization rate to common whelk was 4.9% in allergy patients. Further stuides are needed to evaluate the clinical significance of sensitized patients to common whelk, and to confirm a cross reactivity with abalone. (J Asthma Allergy Immunol 24 : 77-84, 2004)