녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

영도구의 생식보건사업 성과분석 : 남자중학생을 대상으로 한 성교육 효과 분석을 중심으로 the Effects of Sex Education on Middle School Students

남은우,강혜숙,박성빈,최재임,민체류 고신대학교 영도발전연구소 2001 영도연구 Vol.3 No.-

The purpose of this study was to reduce the sexual problems of middle school students in Youngco-Gu, Busan. The survey was conducted by content analysis which was related with reproductive healty services such as budget, manpower, etc. This study was also conducted with sex education as the object and to measure the effects of sex education. Questionnaires from 203 students(7th-9th graders) in a male middle school were surveyed on Sept. 12-14, 2001. The statistical analysis used were t-test, paired t-test, one-way ANOVA and χ^2 test by using SPSS/PC+ WIN 8.0 program. The results of this study are summarized as follows: First, 66.2% of the students among the subjects had a religion, and of those 50% were buddhists, 34.4% christians, 9.5% catholic. Religion of sexual related matters showed no significant effect. Of these students 68.1% showed medium interest in sexual related matters, 18% little interest or none and 13.5% high interest. 8th graders seemed to show more interest in sexual related matters than other grades. 41.8% of Students seemed to get information on sexual matters from friends and 27.1% from the internet. 30.9% of the respondents talked about their boy/girl friends with their parents compared to 43.3% who chose to talk to their friends about their sexual related problems. Second, 56.7% logged into lascivious-sites on the internet. Of those who logged onto lascivious-sites 46.1% logged in once or twice a month, 31.9% once or twice a week, and 2.7% logged in daily, 69.2% Felt curiosity, 23.4% felt indecent and 7.5% felt like engaging in a sexual act after visiting one of these sites. Third, the effects of sex education on experimental groups of 7th graders showed a 2.1 point increase(p<0.1), 4 point increase for 8th graders(p<.001), and 9th graders increased by 3.3 points(p<.001). Therefore this showed that it was advantageous for students to have sex education. Fourth, the effects of sex education on the students attitude showed only a little more improvement than before in the controlled groups whereas the experimental groups showed a large increase in good attitude in a good direction. Therefore, the experimental groups showed better results in sexual attitude compared to the controlled groups(p<.01).

IPS Empress 도재관의 파절강도 : 하악 중절치에서 절단연 삭제량과 측면 경사도에 따른 영향

남영성,김계순,정영찬,김유리,동진근 원광대학교 치의학연구소 2003 圓光齒醫學 Vol.12 No.1

The purpose of this study was to compare the fracture strength of the IPS Empress ceramic crown according to the incisal depth (1.5mm, 2.0mm, 2.5mm) and axial inclination (4˚, 8˚, 12˚) of the lower central ncisor. After 10 metal dies were made for each group, the IPS Empress ceramic crowns were fabricated and each crown was cemented on each metal die with resin cement. The cemented crowns mounted on the testing jig were inclined 30 degrees and a universal testing machine was used to measure the fracture strength. The results of this study were as follows : 1. The fracture strength of the ceramic crown with 2.0mm depth and 12˚ inclination was the highest (648 N). Crowns of 1.5mm depth and 4˚ inclination had the lowest strength (482 N). 2. There were no significant differences of the fracture strength by axial inclination in same incisal depth group. 3. The fracture mode of the crowns was similar. Most of fracture lines began at the loading area and extended through proximal surface perpendicular to the margin irrespective of incisal depth. There had correlation between fracture strength and fractured surface area.

토양에서 클로르피리포스의 동력학적 거동

유현철,신남철,우성훈,박승조 東亞大學校 附設 環境問題硏究所 2004 硏究報告 Vol.26 No.-

We have investigated physicochemical characteristics and behavior of chlorpyrifos(CPF) in the agricultural soil, soil + dry waste food(DWF) and soil +manure. Experiments were accomplished biological degradation of samples loaded with chlorpyrifos in incubator at 27℃ during 30days in laboratory. In this study, Sample containing soil + manure has shown to be better biological degradation than sample of soil + DWF. The kinetics of chlorpyrifos oxidation catalyzed by agricultural soil, soil + DWF, and soil + manure can be described by Henri-Michaelis-Menten equation, V=(Vmax)/( Km+ C), where Vmax is the maximum velocity and Km the concentration of the substrate(C) corresponding to an initial(V) half of Vmax. The value of Vmax and km is 0.0588㎍CPF/g.soil.hr and 7.3529㎍CPF/g.soil in agricultural soil, 0.0601㎍CPF/g.soil∙hr and 4.4117㎍CPF/g.soil in the soil loaded DWF and 0.0890㎍CPF/g.soil∙hr and 4.2818㎍CPF/g.soil in the soil added manure. Therefore the order of Vmax. were Manure> DWF> Agricultural soil.

유해화학물질의 계통분석

박성우,박유신,이진훈,유재훈,김유나,오세진,이진숙,김남이,박종신,남금문,노동석 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

나노크기의 은 촉매제조 및 특성분석

정유성,이상량,신재순,김영철,박남국 한국공업화학회 2002 응용화학 Vol.6 No.2

Nano-sized silver catalysts have been prepared by alcohol and dioctyl esterof sodium sulphosuccinic acid, anionic surfactant, Aerosol OT(AOT). TOD/TPR andethylene oxide activity have been studied to investigate the characteristics of silvercatalysts. The sizes of Ag particles were estimated to be 10∼16 nm and the peak ofsilver oxide was not observed by XRD except for metallic silver. The desorptionpeak of molecular oxygen by TPD/TPR uniqely observed at about 400 ℃ in thecatalyst prepared by water-alcohol method. However, in the catalyst prepared bymicroemulsion method, the desorption peaks of molecular and atomic oxygens wereobserved at about 400 ℃ and 600 ℃, respectivly. The catalyst prepared by water-alcohol method showed higher activity ethylene oxide than the catalyst prepared by microemulsion method. It eems due to the larger silver particles and easier desorption of molecular oxygen from the catalyst prepared by water-alcohol method.

5세 남아의 Influenza 종격동기종 및 대엽성 폐렴 1례

오희성,한유진,연은경,선동신,송준환,박경배,박준수,김영통,남해선,최영진,김용배 순천향의학연구소 2009 Journal of Soonchunhyang Medical Science Vol.14 No.3S

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특수공작기계에서 쇼트피닝기의 특성 및 투사속도에 관한 연구

노승남,이승호,서유원 서울산업대학교 산업대학원 2000 大學院論文集 Vol.7 No.-

As developing the technology in industry, the materials with superior grade of stiffness and lightness are required, and hence the higher fatigue strength characteristics are necessary. Thus, the non-traditional machining to increasing fatigue strength is becoming influence as very important technology. Shot peening process is essential to ensure repeatability and uniformity on a part to part and lot to lot basis, while conforming to the applicable specifications. The shot material, size, shape and hardness as well as velocity and impact angle must be rigidity controlled to provide consistency in peening results. High velocity of the shot ball increases the impact energy, the shot peening intensity increase. Comparing with the are height for the velocity 42m/sec, the are height for 56m/sec and 70m/sec increase 33% and 48% respectively.

건조제를 이용한 벽부형진열장 밀폐도 간접측정

유혜선,김명남,이승은 국립중앙박물관 2001 박물관보존과학 Vol.3 No.-

박물관 진열장의 기밀성은 일반적으로 CO₂ 추적가스를 이용한 밀폐도를 측정함으로서 조사되어 왔지만 진열장 내부에 구멍을 내야하는 점은 사용 전이나 사용 중인 진열장에 대한 밀폐도 측정에 적용하는데 문제점으로 지적되어 왔다. 이에 진열장의 형상을 변화시키지 않고, 진열장의 밀폐도를 간접측정함으로서 사용 전이나 필요에 따라서는 전시중인 진열장에 대한 밀폐도를 측정할 수 있는 대안을 제시하고자 하였다. 밀폐도 간접측정법은 진열장 내부의 온습도 변화와 습도조절제(Art-sorb)의 중량변화를 통해 진열장의 기밀도를 간접적으로 알아보는 방법이다. 실험은 동일장소에 시공된 3개의 벽부형진열장을 대상으로 진열장내 조명을 켜지 않고 Art-sorb와 온습도 수집기를 넣고 12일간 진행하였다. 각 진열장내 온도와 습도 변동분을 주변변동분으로 나눠 변동율로 나타냈으며, 그 결과 온도 변동율에서는 벽부장A(0.67)가 벽부장B(0.69)나 벽부장C(0.79)보다 변동율이 적었으며 습도 변동율에서도 벽부장A(0.12)가 벽부장B(0.19)나 벽부장C(0.72)보다 변동율이 적어 기밀도가 가장 양호함을 알 수 있었다. 또한 CO₂추적가스를 이용한 직접 밀폐도 측정에서의 공기교환율에 대하여 간접 밀폐도 측정에서의 습도변동율을 대입해 보았다. The airtightness of museum cases in museums was measured by using CO₂ tracing gas, but it has been pointed that it has a problem with measuring the airtightness of a museum case which has a hole inside before it is used or while is being used. So studies tried to come up with alternatives which make it possible to measure the airtightness of a museum case before it is used or while it is being used if necessary by indirectly measuring the airtightness of the museum case without changing its form. Indirect measuring is done by measuring the change of Art-sorb weight and the change of temperature and humidity inside the museum case. Experiments were made for 12 days with three builtin wall case installed at the same place without turning on the lights but with Art sorb and the data logger of temperature and humidity put into museum case. The change rate was produced with the change of temperature and humidity inside the museum case divided by that of those around museum case. As a result, the temperature change rate of museum case A(0.67) was lower than that of museum case B(0.69) or museum case C(0.79). The humidity change rate of museum case A(0.12) was lower than that of museum case B(0.19) or museum case C(0.72) showing that its airtightness was the best. Also in the direct airtightness measurement by CO₂ tracing gas, the air exchange rate was compared with the humidity change rate by Art-sorb in the indirect measurement.

랫드에서 단일시험법을 이용한 반하 물추출물의 생식독성시험

이기남,유욱준,백인정,연정민,남상섭,이범준,남상윤,김윤배,강현규,안병우,김대중,황방연,노재섭,홍정섭,성하정,김순선,조대현,윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

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