발효김치 중 면역활성 젖산균의 분리 및 특성

최재임,강승범,박진호,유성호,윤성식 한국유산균·프로바이오틱스학회 2014 Current Topic in Lactic Acid Bacteria and Probioti Vol.2 No.1

Isolation of Lactobacillus plantarum FBT215 (L. plantarum FBT215) from fermenting kimchi, which has immu-nostimulatory effects, was characterized in this study. To isolate probiotic lactic acid bacteria (LAB) from kimchi, properlydiluted kimchi samples were spread on MRS agar incorporated BCP and sodium azide. One hundred fifty and four LABisolates were isolated, screened for immune-stimulatory activities in murine RAW 264.7 cells. Among the 24 LAB strainsafter MTT assay, FBT215 has shown the best cytokine activity of expression of 676.9±22.2pg/mL of interleukin IL-1α and845.2±31.6pg/mL of TNF-α. For identification, the typing experiments using 16S rDNA sequencing and API 50 CH kitconfirmed that the FBT215 belongs to Lactobacillus plantarum, shortly called as L. plantarum FBT215. The FBT215 strainshowed high antibiotic susceptibility, weak acid tolerance, bile salt tolerance and BSH activity but did not produce bacteri-ocin against indicator strains tested. API ZYM kit analysis resulted that the L. plantarum FBT215 produces seven differentkinds of enzymes. In conclusion, these properties would be used as a functional probiotic culture in the manufacture of fer-mented foods.

Outbreak of Pseudomonas Oryzihabitans Pseudobacteremia Related to Contaminated Equipment in an Emergency Room of a Tertiary Hospital in Korea

우광숙,최재임,Bo-Ram Kim,Ji-Eun Kim,김경희,Jeong man Kim,한진영 대한감염학회 2014 Infection and Chemotherapy Vol.46 No.1

Pseudomonas oryzihabitans is frequently found in various sites within hospital settings, including sink drains and respiratorytherapy equipment. Although it rarely causes human infections, P. oryzihabitans has recently been considered a potential nosocomialpathogen, especially in immunocompromised hosts. We report our experience of an outbreak of P. oryzihabitans pseudobacteremia,presumably due to faulty aseptic preparation of a saline gauze canister.

Urinary Neutrophil Gelatinase-Associated Lipocalin Levels in Comparison with Glomerular Filtration Rate for Evaluation of Renal Function in Patients with Diabetic Chronic Kidney Disease

우광숙,최재임,김보람,김지은,안원석,한진영 대한당뇨병학회 2012 Diabetes and Metabolism Journal Vol.36 No.4

Background: Neutrophil gelatinase-associated lipocalin (NGAL) is a promising biomarker of acute kidney injury. There is a growing body of evidence suggesting that NGAL is also a marker of kidney disease and severity in chronic kidney disease (CKD). We studied the utility of urinary NGAL in more accurately predicting renal function in patients with diabetic CKD. Methods: We studied possible relationships between urinary NGAL, estimated glomerular filtration rate (eGFR), and proteinuria in diabetic CKD patients and in healthy populations. Results: Urinary NGAL levels were significantly higher in CKD patients than in healthy controls (96.0 [2.7 to 975.2] ng/mL vs. 18.8 [1.3 to 81.9] ng/mL, P=0.02), and the GFR was lower among CKD patients (49.3 [13.1 to 78.3] mL/min/1.73 m2 vs. 85.6 [72 to 106.7] mL/min/1.73 m2, P<0.0001). The urinary NGAL level showed a significant inverse correlation with GFR (r=-0.5634, P<0.0001). The correlation analyses between urinary protein level and urinary NGAL levels and GFR were as follows: urine protein and urinary NGAL (r=0.3009, P=0.0256), urine protein and GFR (r=-0.6245, P<0.0001), urine microalbumin and urinary NGAL (r=0.1794, P=0.2275), and urine microalbumin and GFR (r=-0.5190, P=0.0002). Conclusion: From these results, we concluded that urinary NGAL is a reliable marker of renal function in diabetic CKD patients. However, urinary NGAL did not provide more accurate information regarding renal function than GFR.

Identification of Lactobacillus spp. associated with nematodes in peach farm soil

이우현,최재임,이진일,이원표,윤성식,Lee, Woo-Hyun,Choi, Jae Im,Lee, Jin Il,Lee, Won-Pyo,Yoon, Sung-Sik The Microbiological Society of Korea 2017 미생물학회지 Vol.53 No.3

Strains D4 and D5 were isolated from peach-rotten soil during the peach harvest season. The isolates were identified based on morphological and biochemical characterization, and identification was determined by 16S rRNA gene sequencing. Results showed that D4 has high similarity to Lactobacillus plantarum ATCC $14917^T$ and Lactobacillus pentosus ATCC $8041^T$ at 99.05% and 98.98%, respectively. D5 was also similar to Lactobacillus pentosus ATCC $8041^T$ and Lactobacillus plantarum ATCC $14917^T$ at 98.71% and 98.64%, respectively. In contrast, isolates showed differences in carbohydrate utilization in comparison to Lactobacillus plantarum ATCC $14917^T$ and Lactobacillus pentosus ATCC $8041^T$. In view of this we performed VITEK MS matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis, multiplex PCR fingerprinting, and random amplified polymorphic DNA (RAPD)-PCR to further confirm the identification of D4 and D5. The results of these analyses showed that both strains were most similar to Lactobacillus plantarum. 복숭아 수확시기에 낙과한 토양에서 Lactobacillus sp. D4와 D5 균주를 분리하였다. 분리한 Lactobacillus sp. D4와 D5 균주를 동정하기 위하여 형태학적 동정, 생화학적 동정 및 16S rRNA 유전자서열 분석을 수행하였다. 16S rRNA 유전자서열 분석 결과 Lactobacillus sp. D4는 Lactobacillus plantarum subsp. plantarum ATCC $14917^T$과 Lactobacillus pentosus ATCC $40997^T$에 각각 99.05%, 98.98% 일치하였으며, Lactobacillus sp. D5는 Lactobacillus pentosus ATCC $40997^T$, Lactobacillus plantarum subsp. plantarum ATCC $14917^T$에 각각 98.71%, 98.64% 일치하였다. Lactobacillus sp. D4와 D5 균주는 당 이용성 비교에서 Lactobacillus plantarum ATCC $14917^T$과 Lactobacillus pentosus ATCC $8041^T$에 비교하여 다른 결과를 나타내었다. 정확한 동정을 위하여 VITEK MS matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) 분석, multiplex PCR, random amplified polymorphic DNA (RAPD)-PCR을 수행하였다. 이러한 결과에 근거하여 Lactobacillus sp. D4와 D5 균주는 Lactobacillus plantarum으로 동정되었다.

Clinical Usefulness of Serum Cystatin C as a Marker of Renal Function

우광숙,최재임,김보람,김지은,한진영 대한당뇨병학회 2014 Diabetes and Metabolism Journal Vol.38 No.4

Background: Accurate renal function measurements are important in the diagnosis and treatment of kidney diseases. In contrast to creatinine, the production of serum cystatin C has been extensively reported to be unaffected by body muscle mass, age, gender, and nutritional status. Methods: Our study included 37 samples from diabetic chronic kidney disease (CKD) patients for whom serum creatinine tests had been requested and 40 samples from a healthy populations in Dong-A University Hospital between May 2010 and June 2010. The assay precision (i.e., the coefficient of variation) and the reference range of the serum cystatin C test were evaluated. We compared the estimated glomerular filtration rates (GFRs) based on cystatin C with those based on creatinine. Moreover, we investigated the influences of age, gender, weight, and muscle mass on serum creatinine and serum cystatin C. Results: There was a positive correlation between GFR based on creatinine and that based on cystatin C (r=0.79, P<0.0001) among the diabetic CKD patients. Serum creatinine and cystatin C were significantly correlated with body weight and muscle mass, but the strengths of these correlations were greater for serum creatinine. The precision study revealed excellent results for both the high and low controls. The 95% reference interval of cystatin C in the healthy population was 0.371 to 1.236 mg/L. Conclusion: Based on these results, we conclude that, despite the strong correlation between serum creatinine and cystatin C, cystatin C is less affected by weight and muscle mass and might represent a better alternative for the assessment of renal function.

Correlation Between the CYP2C19 Phenotype Status and the Results of Three Different Platelet Function Tests in Cardiovascular Disease Patients Receiving Antiplatelet Therapy: An Emphasis on Newly Introduced Platelet Function Analyzer-200 P2Y Test

Shuhua Li,최재임,Long Zhe Guo,Ri-Young Goh,김보람,우광숙,김무현,한진영 대한진단검사의학회 2016 Annals of Laboratory Medicine Vol.36 No.1

Background: An association has been reported between CYP2C19 polymorphism and the altered antiplatelet activity of clopidogrel. We investigated this association using the newly introduced platelet function analyzer (PFA)-200 (INNOVANCE PFA-200 System; Siemens Healthcare, Germany) P2Y test. Methods: Polymorphisms of CYP2C19*2, *3, *17 and the degree of inhibition of platelet function were determined in 83 patients. Three different platelet function tests were used to evaluate the degree of platelet inhibition and to check the association with genotype. Results: The post-procedure PFA-200 values of extensive metabolizers (EM) patients (285.3±38.8) were higher than those of intermediate metabolizers (IM) and poor metabolizers (PM) patients (227.7±98.3 and 133.7±99.2, respectively; P=0.024). Light transmittance aggregometry (LTA) and the VerifyNow system showed that the post-procedure values for EM patients were lower than those of IM and PM patients (LTA: 24.4±15.7, 34.1±17.6, and 42.2±16.9, respectively, P<0.001; VerifyNow: 133.2±60.5, 171.5±42.6, and 218.7±59.3, respectively, P<0.001). The high residual platelet reactivity (HPR) rates were significantly different among the EM, IM, and PM groups using PFA-200 (PM:IM:EM=82.4:40.6:11.8, P<0.001). Conclusions: Approximately, 59.0% of Korean patients with cardiovascular disease receiving clopidogrel had CYP2C19 loss-of-function genotypes classified as IM or PM, and the frequency was similar to the data from Asian people. The PFA-200, LTA, and VerifyNow platelet function tests revealed evidence of a significant association between the efficacy of clopidogrel and CYP2C19 genotypes.

남녀 중ㆍ고교 학생들의 성지식 및 의식에 관한 연구

강혜숙,남은우,박성빈,최재임 한국보건교육건강증진학회 2000 한국보건교육·건강증진학회 학술대회 발표논문집 Vol.- No.-

영도구의 생식보건사업 성과분석 : 남자중학생을 대상으로 한 성교육 효과 분석을 중심으로 the Effects of Sex Education on Middle School Students

남은우,강혜숙,박성빈,최재임,민체류 고신대학교 영도발전연구소 2001 영도연구 Vol.3 No.-

The purpose of this study was to reduce the sexual problems of middle school students in Youngco-Gu, Busan. The survey was conducted by content analysis which was related with reproductive healty services such as budget, manpower, etc. This study was also conducted with sex education as the object and to measure the effects of sex education. Questionnaires from 203 students(7th-9th graders) in a male middle school were surveyed on Sept. 12-14, 2001. The statistical analysis used were t-test, paired t-test, one-way ANOVA and χ^2 test by using SPSS/PC+ WIN 8.0 program. The results of this study are summarized as follows: First, 66.2% of the students among the subjects had a religion, and of those 50% were buddhists, 34.4% christians, 9.5% catholic. Religion of sexual related matters showed no significant effect. Of these students 68.1% showed medium interest in sexual related matters, 18% little interest or none and 13.5% high interest. 8th graders seemed to show more interest in sexual related matters than other grades. 41.8% of Students seemed to get information on sexual matters from friends and 27.1% from the internet. 30.9% of the respondents talked about their boy/girl friends with their parents compared to 43.3% who chose to talk to their friends about their sexual related problems. Second, 56.7% logged into lascivious-sites on the internet. Of those who logged onto lascivious-sites 46.1% logged in once or twice a month, 31.9% once or twice a week, and 2.7% logged in daily, 69.2% Felt curiosity, 23.4% felt indecent and 7.5% felt like engaging in a sexual act after visiting one of these sites. Third, the effects of sex education on experimental groups of 7th graders showed a 2.1 point increase(p<0.1), 4 point increase for 8th graders(p<.001), and 9th graders increased by 3.3 points(p<.001). Therefore this showed that it was advantageous for students to have sex education. Fourth, the effects of sex education on the students attitude showed only a little more improvement than before in the controlled groups whereas the experimental groups showed a large increase in good attitude in a good direction. Therefore, the experimental groups showed better results in sexual attitude compared to the controlled groups(p<.01).

영도지역 남녀 중ㆍ고교 학생들의 성지식에 관한 연구

강혜숙,남은우,박성빈,최재임 고신대학교 영도발전연구소 2000 영도연구 Vol.2 No.-

The purpose of this study was to grasp the sex knowledge in middle and high school students in Youngdo-Gu, Busan, and to provide some basic data for the needs and the direction of sex education for the teenagers. The survey was conducted by using questionnaires in two middle schools and two high schools in Youngdo-Gu. The questionnaires were took back after record by the respondents. The survey was conducted at September 15∼17, 2000 and a total of 791 were completed. The respondents were 200 (25.3%) males and 199 (25.2%) females in middle schools and 196 (24.8%) males and 196 (24.8%) females in high schools. For the statistical analysis, χ2-test, t-test and one-way ANOVA were used by using SPSS/PC+ WIN 8.0 program. The conclusions based on the results obtained from this study are as follows: 1) An attitude of the sexual matters showed that boys, students and non-christians were more progressive than girls, parents and christians, respectively (p<0.01). 2) The sexual agonies of students were in order of having a date 36.2%, sexual drive 11.1%, sexual intercourse 8.8%, masturbation 8.6% and sexual abuse 6.7%. 3) The points for sexual knowledges appeared 40.6 points out of 100 points from boys and 42.3 points from girls in middle schools, and 57.3 points from boys and 66.1 points from girls in high schools. Therefore, the girls had much knowledges on sexuality than those of the boys. There were a significant differences of knowledge on sexuality between boys and girls (p<0.001). Over 60% of both boys and girls had not enough knowledges on sexually transmitted diseases (STDs). 4) Satisfaction of sex education was common or unsatisfied as over 90% of respondents. The reasons were a textbook-like general information (50%) and nothing practically to help (29%). 5) The students wanted sex education for the practice in future life such as male and female relationship with etiquette(23%); and pregnancy and delivery (17%) in middle school students, and contraceptive and abortion (34%) and preventive STDs (18%). There was not enough to understand whole adolescent in Youngdo area because the study subjects were only public middle and hight school students in Youngdo-Gu, Busan. Conclusively, it is necessary to educate to give right information of sexuality to the adolescents in Youngdo, and to have systematically sex education from the kindergartens to the high schools by long range sex educational program. Also, a proper program for integrated sex education is necessary to build healthful sexual consciousness and worth in cooperation with sex educators, universities and health centers.