이락촌폐흡충의 조직내 항원성부위의 미세구조적 관찰

남해선 순천향대학교 1994 논문집 Vol.17 No.4

An immunoelectron microscopic observation of various antigenic sites of the lung fluke, adult Paragonimus iloktsuenensis, was performed by applying the indirect immunoperoxidase technique to localize the selective antigenic sites. The fixative was mixture of 1% paraformaldehyde and 0.2% glutaraldehyde in 0.1M phosphate buffer, pH 7.4. LR-white resin(EMS, hard grade), the embedding media, was polymerized at -20℃ under the ultraviolet light(365nm long-wave length) for 48 hours. Nickel grids(EMS, 200 mesh) were coated with 2% collodion(in isoamyl-acetate) and 0.5% polyvinyl formvar. The antiserum produced from the rat infected with Paragonimus iloktsuenensis(10 weeks after infection) was used as a primary antibody. The secondary antibody was gold conjugated goat anti-rat IgG(particle size=10nm, Sigma). The intestinal epithelial cells containing numerous antigenic granules which seem to be secreted into the intestinal lumen. The secretory granules and luminal contents were predominantly labeled by gold particles. The excretory bladder was also proved to be a highly antigenic organ, since its lamellated structures and contents were labeled by numerous gold particles. The antigenicities of secretory materials of the vitelline glands, testes, and ovaries were moderately recognizible, too. But the tegument was very weakly labeled by gold particles. The above results suggests that the secretory substances from the intestinal epithelial cells and excretory contents from excretory bladder in the adult Paragonimus iloktsuenensis are the most potent antigenic stimulants to the host immune system.

대호만 참붕어의 흡충류 피낭유충 감염상

남해선 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

This study was done in December 1998, for research on the actual condition of the infection status of trematode metacercariae in Pseudorasbora parva caught from Daehoman(pond) located in Sosan-Si, Chungnam. Total of 73 Pseudorasbora parva were examined under stereomicroscope after artificial digestion with pepsin-HCl solution. Three species of metacercariae were found, and each numbers of collected total metacercariae were 157 of Clonorchis sinensis, 124 of Methorchis orientalis and 39,092 of Cyathocotyle orientalis. The average numbers of detected metacercariae per each Pseudorasbora parva were Clonorchis sinensis 2.2, Methorchis orientalis 1.7 and Cyathocotyle orientalis 535.5.

알레르기 환자에서 MAST-CLA 검사의 의의

남해선,황규윤,,백병준,정윤영,오천환,조성란,박준수 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

To evaluate usefulness of application of MAST-CLA to indetify allergens, authors conducted MAST-CLA allergen-specific IgE assay and collected allergy related symptoms in 118 allergic patients diagnosed at Soonchunhyang University Chunan Hospital during October 1998 through January 2000. The results were as followings; 1. The mean (SD) age was 23.1 (±12.17) years old and teenagers accounted for 33.1% of patients. 2. The frequent allergic symptoms were sneezing (59.3%), rhinorrhea (53.4%), nasal stiffness (44.1%), itching sensation (24.6%), cough or sputum (16.1%), and eye symptom (8.5%) in order. 3. Among these subjects, 62.7% of patients revealed at least more than one specific allergen and 70.3% showed elevated total IgE levels by MAST-CLA. The three most frequent specific allergens were Dermatophagoides farinae (47.5%), Dermatophagoides pteronyssinus (45.8%), and house dust (30.5%). 4. While age was not significantly related to positive of both specific allergens and total IgE (P>0.05), sneezing and nasal stiffness were significantly associated with positive of specific allergen and total IgE, respectively (both P<0.05). 5. Total IgE increased with the number of specific allergens, but both specific allergen and total IgE were not significantly related to increases of allergic symptoms (P>0.05). In conclusion, our data suggest that MAST-CLA allergen-specific IgE assay is useful measure for identification of causative allergens in allergic patients, however, it is recommended that more specific allergic antigens should be determined and replaced or added to Korean inhalent panel of MAST-CLA.

알레르기 환자에서 혈청 IgE와 특이항원 양성율의 일치도

남해선,권준택,박준수 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

To evaluate usefulness of serum IgE to determine specific allergens, authors conducted priest IgE and MAST-CLA allergen-specific IgE assays in 78 allergic patients diagnosed at Soonchunhyang University Chunan Hospital during October 1998 through July 2000. During the study period, inhalent and food Korean panels were applied in 57 (73.1%) and 21 outpatients (26.9%), respectively. Kappa index was calculated to evaluate the agreement of priest IgE with Total IgE and allergen-specific IgE by MAST-CLA. 1. The men (±SD) age was 13.2 (±14.1) year-old and male accounted for 62.8%. 2. The overall positive rate of priest IgE was 66.7% and mean (±SD) level of priest IgE was 271.2 (±265.6) IU/ml 3. Patients with more than one specific allergen and with elevated total IgE by MAST-CLA were 25 (32.1%) and 33 (42.3%), respectively. 4. Significant agreement between total IgE and allergen-specific IgE was shown, but strength of agreement was slight (Kappa = 0.186, P = 0.046), 5. While no significant agreement between priest IgE and total IgE was observer, the priest IgE agreed significantly with allergen-specific IgE (Kappa = 0.153, P=0.043) In conclusion, our data suggested that serum priest IgE is more likely associated with allergen-specific IgE than total IgE in MAST-CLA assay. Furthermore, it is recommended that Korean panel of MAST-CLA should contain more specific allergens.

알레르기성 소인이 없는 정상인에서 특이 알레르겐항체 양성율

남해선,안현철,박준수,황규윤,박춘식 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

This study was designed to evaluate prevalence of allergen-specific antibodies in healthy adults. During Mar. ~ Aug. 2001, 1,925 subjects visited for medical check-up, were inquired to fill out a standardized questionnaire. Among them, 200 subjects who had no current and past allergic disorders were randomly selected for rhe current study and examined for allergen-specific antibodies using Korean inhalant panel of MAST-CLA allergen-specific IgE assay. Among them, 170 persons (85.0%) did not have any positive allergen-specific antibody. Each positive rate of 35 allergen specific antibodies was usually less than 3%, except Cockroach Mix (3.5%), Dermatophagoides farinae(8.5%), Dermatophagoides pteronyssinus(6.0%). Among 30 positive subjects, 19(63.3%) showed one or two allergen-specific antibodies. Although atopic subjects in non-allergic population were not common, few of them showed allergen-specific antibodies in Korean inhalant panel of MAST-CLA. Our results suggested that the standardized questionnaire and MAST-CLA could be an useful tool of a screening test for allergy, showing high specificity and sensitivity.

Immunoblot Technique을 이용한 간흡충증 진단에 관한 연구

남해선,이옥란 순천향대학교 1990 Journal of Soonchunhyang Medical Science Vol.13 No.1

세로무늬 먼지진드기의 실험실적 배양에서 각종 천연재료의 효과

남해선,이상한 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.2

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일반 스폰지베개 및 깃털베개 커버의 집먼지와 집먼지진드기 투과성

남해선 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Previous studies have demonstrated significantly higher house dust mite allergen levels on synthetic pillows, compared to feather pillows. Reasons for these differences could be lower permeability of feather pillow coverings to allergen, greater house dust mite penetration of synthetic pillow covering, or a combination of both. This study was done to determine the permeability of synthetic and feather pillow coverings to house dust mites and house dust. Twenty live adult house dust mites were seeded on top of a type of synthetic and a type of feather pillow coverings (all in dupicate) with adequate food supply below in sealed culture dished and kept at 23℃ and 70% relative humidity. After 24hr and 48hr house dust mite numbers remaining on top of the coverings were enumerated microscopically. Aliquots of fine house dust (in triplicate) were placed on top of the synthetic and feather pillow coverings, shaken gently for 30min and penetrated dust collected and weighed. After 24hr, all 20 house dust mites had penetrated the standard synthetic pillow coverings, while no house dust mites had penetrated either the feather pillow after 48hr. Dust permeability for the standard synthetic and feather pillow coverings were 0.88% and 0.07%, respectively. These findings of total permeability of standard synthetic pillow coverings to house dust mites, and their greater permeability to house dust could explain their reported higher house dust mite allergen levels, compared to feather pillow coverings.

유산균 발효유 음용의 인체 항산화 효과

남해선 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

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베개내 세균성 내독소와 집먼지진드기 항원 농도의 비교

남해선 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

베개에는 일반적으로 많은 양의 실내 알레르겐과 세균성 내독소가 있는 것으로 알려져 있으며, 이는 집먼지진드기 항원에 감작되어 있는 사람에게는 매우 중요시해야 하는 요소 중의 하나로 되어 있다. 메밀베개는 한국에서는 일반적으로 사용되고 있는 베개이다. 이번 실험을 통하여 메밀베개와 일반 스폰지 베개 내의 집먼지진드기 항원의 양과 내독소의 양이 사용함에 따라 어떻게 변하는지에 대하여 규명해 보고자 천안시에 거주하는 13가구에서 새로 구입하여 지급한 두가지 타입의 베개를 3개월간 같은 침대에서 사용토록한 후 사용전후의 내독소와 집먼지진드기의 양을 서로 비교하였다. 새 베개에서는 두 가지 타입 모두에서 집먼지진드기(Der f 1 및 Der p 1) 항원이 측정되지 않았다. 3개월간 사용한 후에 측정한 Der f 1 의 농도는 메밀베개와 스폰지베개에서 평균이 각각 1,16 g/g (0.02-8.13)와 1.08 g/g (0.19-1.68)로 95% 신뢰구간에서 유의한 차이가 없었다. 그러나 새로 구입한 메밀베개와 스폰지베개에서 측정한 내독소의 양은평균이 각각 60.950 EU/g (30,270-122,700) 와 4,887 EU/g (2,570-9,311) 로 95%의 신뢰구간에서 유의한 차이가 있었다. 3개월간 사용한 후에 측정된 Der f 1 의 양은 메밀베개와 스폰지 베개에서 평균이 각각1.16 g/g (0.02-8.13) 과 1.08 g/g (0.19-1.68) 로 95% 신뢰도 구간에서 유의한 차이가 없었다. 이러한 결과를 통하여 메밀베개는 매우 많은 양의 내독소를 함유하고 있어서 심한 알레르기성 천식의 유발원인이 될 수 있음을 확인하였다.