Pinoresinol-4,4'-di-O-β-d-glucoside from Valeriana officinalis root stimulates calcium mobilization and chemotactic migration of mouse embryo fibroblasts

Do, K.H.,Choi, Y.W.,Kim, E.K.,Yun, S.J.,Kim, M.S.,Lee, S.Y.,Ha, J.M.,Kim, J.H.,Kim, C.D.,Son, B.G.,Kang, J.S.,Khan, I.A.,Bae, S.S. G. Fischer 2009 Phytomedicine Vol.16 No.6

Lignans are major constituents of plant extracts and have important pharmacological effects on mammalian cells. Here we showed that pinoresinol-4,4'-di-O-β-d-glucoside (PDG) from Valeriana officinalis induced calcium mobilization and cell migration through the activation of lysophosphatidic acid (LPA) receptor subtypes. Stimulation of mouse embryo fibroblast (MEF) cells with 10μM PDG resulted in strong stimulation of MEF cell migration and the EC<SUB>50</SUB> was about 2μM. Pretreatment with pertussis toxin (PTX), an inhibitor of G<SUB>i</SUB> protein, completely blocked PDG-induced cell migration demonstrating that PDG evokes MEF cell migration through the activation of the G<SUB>i</SUB>-coupled receptor. Furthermore, pretreatment of MEF cells with Ki16425 (10μM), which is a selective antagonist for LPA<SUB>1</SUB> and LPA<SUB>3</SUB> receptors, completely blocked PDG-induced cell migration. Likewise, PDG strongly induced calcium mobilization, which was also blocked by Ki16425 in a dose-dependent manner. Prior occupation of the LPA receptor with LPA itself completely blocked PDG-induced calcium mobilization. Finally, PDG-induced MEF cell migration was attenuated by pretreatment with a phosphatidylinositol 3-kinase (PI3K) inhibitor such as LY294002. Cells lacking downstream mediator of PI3K such as Akt1 and Akt2 (DKO cells) showed loss of PDG-induced migration. Re-expression of Akt1 (but not Akt2) completely restored PDG-induced DKO cell migration. Given these results, we conclude that PDG is a strong inducer of cell migration. We suggest that the pharmacological action of PDG may occur through the activation of an LPA receptor whereby activation of PI3K/Akt signaling pathway mediates PDG-induced MEF cell migration.

표면항원 및 유전자분석에 의한 V. vulnificus균의 계통분류학적 연구(Ⅰ) : 지방산 조성 및 RAPD PCR에 의한 V. vulnificus균의 계통분류학적 연구

윤영희,김기상,신영학,이점규,오경수,유정식,이상원,이근용 국립보건연구원 1998 국립보건원보 Vol.35 No.-

Effects of Organic or Inorganic Acid Supplementation on Growth Performance, Nutrient Digestibility and White Blood Cell Counts in Weanling Pigs

D. Y. Kil,L. G. Piao,H. F. Long,J. S. Lim,M. S. Yun,C. S. Kong,W. S. Ju,H. B. Lee,Y. Y. Kim 아세아·태평양축산학회 2006 Animal Bioscience Vol.19 No.2

Four experiments were conducted to investigate the effect of organic or inorganic acid supplementation on the growth performance, nutrient digestibility, intestinal measurements and white blood cell counts of weanling pigs. In growth trial (Exp I), a total of 100 crossbred pigs ({Landrace횞Yorkshire}횞Duroc), weaned at 23짹2 days of age and 7.25짹0.10 kg average initial body weight (BW), were allotted to 5 treatments by body weight and sex in a randomized complete block (RCB) design. Three different organic acids (fumaric [FUA], formic [FOA] or lactic acid [LAA]) and one inorganic acid (hydrochloric acid [SHA]) were supplemented to each treatment diet. Each treatment had 5 replicates with 4 pigs per pen. During 0-3 wk, average daily gain (ADG), average daily feed intake (ADFI) and feed efficiency (G/F ratio) were not significantly different among treatments. However, pigs fed LAA or SHA diet showed improved ADG by 15 or 13% respectively and 12% greater ADFI in both treatments compared to CON diets. Moreover, compared to organic acid treatments, better ADG (p = 0.07) and ADFI (p = 0.09) were observed in SHA diet compared to pigs that were fed the diet containing organic acids (FUA, FOA or LAA). However, during 4-5 wk, no differences in ADG, ADFI and G/F ratio were observed among treatments. Overall, ADG, ADFI and G/F ratio were not affected by acidifier supplementation. Although it showed no significant difference, pigs fed LAA or SHA diets showed numerically higher ADG and ADFI than pigs fed other treatments. In metabolic trial (Exp II), 15 pigs were used to evaluate the effect of acidifier supplementation on nutrient digestibility. The digestibility of dry matter (DM), crude protein (CP), crude fat (CF), crude ash (CA), calcium (Ca) and phosphorus (P) was not improved by acidifier supplementation. Although the amount of fecal-N excretion was not different among treatments, that of urinary-N excretion was reduced in acidsupplemented treatments compared to CON group (p = 0.12). Subsequently, N retention was improved in acid-supplemented groups (p = 0.17). In anatomical trial (Exp III), the pH and Cl- concentrations of digesta in gastrointestinal (GI) tracts were not affected by acidifier supplementation. No detrimental effect of intestinal and lingual (taste bud) morphology was observed by acidifier supplementation particularly in inorganic acid treatment. In white blood cell assay (Exp IV), 45 pigs were used for measuring white blood cell (WBC) counts. In all pigs after LPS injection, WBC counts had slightly declined at 2 h and kept elevating at 8 h, then returned to baseline by 24 h after injection of lipopolysaccharide (LPS). However, overall WBC counts were not affected by acidifier supplementation. In conclusion, there was no difference between organic and inorganic acidifier supplementation in weanling pigs' diet, however inorganic acidifier might have a beneficial effect on growth performance and N utilization with lower supplementation levels. Furthermore, inorganic acidifier had no negative effect on intestinal measurements and white blood cell counts in weanling pigs. These results suggested that inorganic acidifier might be a good alternative to organic acidifiers in weanling pigs.

Inhibitory effect of a novel naphthoquinone derivative on proliferation of vascular smooth muscle cells through suppression of platelet-derived growth factor receptor β tyrosine kinase

Kim, Y.,Han, J.H.,Yun, E.,Jung, S.H.,Lee, J.J.,Song, G.Y.,Myung, C.S. North-Holland ; Elsevier Science Ltd 2014 european journal of pharmacology Vol.733 No.-

This study was designed to investigate the antiproliferative effect of a novel naphthoquinone derivative, 2@?undecylsulfonyl@?5,8@?dimethoxy@?1,4@?naphthoquinone (2-undecylsulfonyl-DMNQ), on platelet-derived growth factor (PDGF)-stimulated vascular smooth muscle cells (VSMCs) and examine the possible molecular mechanism of its antiproliferative action. 2-Undecylsulfonyl-DMNQ significantly inhibited PDGF-stimulated cell number and DNA synthesis, and arrested the PDGF-stimulated progression through G<SUB>0</SUB>/G<SUB>1</SUB> to S phase of cell cycle supported by the suppression of pRb phosphorylation and cyclin D1/E, CDK2/4 and PCNA expressions. 2-Undecylsulfonyl-DMNQ dose-dependently inhibited the PDGF-stimulated phosphorylation of phospholipase Cγ (PLCγ), protein kinase B (Akt/PKB), signal transducers and activators of transcription 3 (STAT3) and extracellular signal-regulated kinase ½ (ERK ½). In addition, 2-undecylsulfonyl-DMNQ inhibited PDGF-induced PDGF receptor β (PDGF-Rβ) dimerization and the phosphorylation of Tyr<SUP>579/581</SUP>, Tyr<SUP>716</SUP>, Tyr<SUP>751</SUP> and Tyr<SUP>1021</SUP> in PDGF-Rβ. However, 2-undecylsulfonyl-DMNQ has no antiproliferative effect on epidermal growth factor (EGF)- or fetal bovine serum (FBS)-stimulated VSMCs. In conclusion, these findings suggest that the antiproliferative effects of 2-undecylsulfonyl-DMNQ on PDGF-stimulated VSMCs are due to the blockade of receptor dimerization and autophosphorylation on specific tyrosine residues of PDGF-Rβ, which resulted in the subsequent suppression of signaling cascades and a cell cycle arrest. Our observation may explain an important mechanism to block the integration of multiple signals generated by growth factor receptor activation for prevention of VSMC proliferation in cardiovascular diseases.

Production of ω-hydroxy palmitic acid using CYP153A35 and comparison of cytochrome P450 electron transfer system in vivo

Jung, E.,Park, B. G.,Ahsan, M. M.,Kim, J.,Yun, H.,Choi, K. Y.,Kim, B. G. Springer Science + Business Media 2016 Applied microbiology and biotechnology Vol.100 No.24

<P>Bacterial cytochrome P450 enzymes in cytochrome P450 (CYP)153 family were recently reported as fatty acid omega-hydroxylase. Among them, CYP153As from Marinobacter aquaeolei VT8 (CYP153A33), Alcanivorax borkumensis SK2 (CYP153A13), and Gordonia alkanivorans (CYP153A35) were selected, and their specific activities and product yields of omega-hydroxy palmitic acid based on whole cell reactions toward palmitic acid were compared. Using CamAB as redox partner, CYP153A35 and CYP153A13 showed the highest product yields of omega-hydroxy palmitic acid in whole cell and in vitro reactions, respectively. Artificial self-sufficient CYP153A35-BMR was constructed by fusing it to the reductase domain of CYP102A1 (i.e., BM3) from Bacillus megaterium, and its catalytic activity was compared with CYP153A35 and CamAB systems. Unexpectedly, the system with CamAB resulted in a 1.5-fold higher yield of omega-hydroxy palmitic acid than that using A35-BMR in whole cell reactions, whereas the electron coupling efficiency of CYP153A35-BM3 reductase was 4-fold higher than that of CYP153A35 and CamAB system. Furthermore, various CamAB expression systems according to gene arrangements of the three proteins and promoter strength in their gene expression were compared in terms of product yields and productivities. Tricistronic expression of the three proteins in the order of putidaredoxin (CamB), CYP153A35, and putidaredoxin reductase (CamA), i.e., A35-AB2, showed the highest product yield from 5 mM palmitic acid for 9 h in batch reaction owing to the concentration of CamB, which is the rate-limiting factor for the activity of CYP153A35. However, in fed-batch reaction, A35-AB1, which expressed the three proteins individually using three T7 promoters, resulted with the highest product yield of 17.0 mM (4.6 g/L) omega-hydroxy palmitic acid from 20 mM (5.1 g/L) palmitic acid for 30 h.</P>

Cartilage Oligometric Matrix Protein-Angiopoietin-1 Promotes Revascularization Through Increased Survivin Expression in Dermal Endothelial Cells of Skin Grafts in Mice

Byun, S.J.,Choi, K.S.,Park, S.H.,Cho, N.W.,Hyun Yoo, C.,Yun, K.J.,Koh, Y.J.,Koh, G.Y.,So, B.J.,Yoon, K.H. American Association of Pathologists and Bacteriol 2007 The American journal of pathology Vol.171 No.5

The present study examined the effects of cartilage oligometric matrix protein angiopoietin-1 (COMP-Ang1) on the revascularization of mice skin grafts. Full-thickness skin grafts were autotransferred into BALB/c mice. The donor grafts were soaked in COMP-Ang1 protein (50 μg/ml, n = 10) or in bovine serum albumin (BSA) (50 μg/ml, n = 10) dissolved in 1 ml of sterile, phosphate-buffered saline for 5 minutes before transfer. Revascularization of the grafts was monitored using an intravital microscope on postoperative days 3, 4, and 5. Morphological and immunohistochemical analyses were performed to evaluate platelet-endothelial cell adhesion molecule-1 and survivin expression and apoptotic signal in the transplanted grafts. Grafts soaked in COMP-Ang1 (COMP-Ang1 group) showed significantly increased revascularization compared with grafts soaked in BSA (BSA group) on intravital microscopy and platelet-endothelial cell adhesion molecule-1 staining. The COMP-Ang1 group showed a significant increase of survivin expression in the endothelial cells and a reduction of apoptotic signal in comparison to the BSA group. Therefore, we believe that COMP-Ang1 provides the therapeutic benefit of enhancing the survival of vascular endothelial cells during transplantation of skin graft.

알파술폰 고급지방산 폴리에틸렌글리콘 에스테르류의 계면물성

윤영균,남기대,김진현,연영흠 한국유화학회 1998 한국응용과학기술학회지 Vol.15 No.2

All the surface activities including surface tension, foaming power, foam stability, emulsifying power, dispersion effect, and detergency were measured and critical micelle concentration(cmc) was evaluated in dilute aqueous solution. The cmc evaluated by the Ring method was 10^-3∼10^-4mol/L in case of monoesters, and 10^-3∼5.0×10^-5mol/L in case of diesters, respectively. Surface tension of the aqueous solution was decreased to 45∼50dyne/㎝, showing the tendency that the ability of lowering the surface tension was dependent on increasing of carbon atom number in alkyl chain. Foaming power of all the monoesters was better than that of diesters. while foam stability of diesters was to the contrary. Emulsifying power of soybean oil or benzene was specially expected to be good for emulsifiers in industrial application fields. HLB values of monoesters and diesters evaluated by Griffin's method were in the range of 8 to 12. Dispersion property of ferric oxide was stable in the range of 4.5×10^-5∼5.0×10^-4mol/L in case of monoesters, and 10^-5∼10^-4mol/L in case of diesters.

PLUG/PLAY 방식 고속 지능형 가공 시스템의 연구

윤원수,김찬봉,권용찬,한기상,양희구,김세광,김주한,박종권 한국공작기계학회 2000 한국공작기계학회 추계학술대회논문집 Vol.2000 No.-

This study aims at developing the high speed/intelligent machining system using the plug/play method of an open architecture controller. The plug/play technology by the Application Specific Function (ASF), can readily implement the open architecture controller into various machining system or other automatic devices. The plug/play method integrates the ASF, visual builder, controller OS technology. This study, as an example, presents a schematic diagram for integration of an open architecture CNC and individual component technology for the high speed/intelligent machining system.

곡물 순환식의 상온통풍 건조기 개발(Ⅰ) : 건조기 개발 및 벼의 건조성능 분석 Development of dryer and analysis of drying performance

윤홍선,정훈,조영길,박원규 한국농업기계학회 2000 바이오시스템공학 Vol.25 No.3

경유의 저온특성에 따른 농용 디젤엔진의 저온시동성

신승엽,김학주,이용복,김병갑,윤진하,김기택,양대준 한국농업기계학회 2003 바이오시스템공학 Vol.28 No.1

This study was carried out to get basic data of troubles in starting and supply of farm diesel engines in cold winter. The results of the study are summarized as follows: 1. As the result of farm survey, the proportions of farms which had starting problems or troubles in fuel supply in cold winter for the last 5 years were 38% for the farms with power-tillers and 32% for the farms with tractors. Most of the farms which had starting problems or troubles in fuel supply in cold winter used light oil for summer, spring or fall rather than for winter. 2. As the result of fuel supply test, fuel supply was stopped at -6˚C and -18˚C for summer light oil and winter light oil, respectively. 3. the lowest temperatures of winter light oil for starting engine were -7.5˚C for power-tiller,-12.5˚C for tractor of 38PS, and -17.5˚C for tractor of 45PS,which were 5~7.5˚C lower than that of summer light oil. 4. The performance of engine starting and the trouble of fuel supply system at lower temperature were significantly improved by using winter hight oil rather than summer light oil.