Protein Methylase Ⅱ 活性 및 그 酵素力學에 미치는 金屬이온의 影響

姜龍淳,林圭,郭相太 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.1

Effects of metal ions on the kinetic properties of protein methylase II from various tissues were investigated. 1. Copper ion(Cu^2+) and zinc ion(Zn^2+) were potent inhibitors, the activity of the enzymes from heart, liver, brain and placenta being completely inhibited at 2 mM. The other ions did not almostly affect the activity of the enzyme. 2. The inhibition of copper and zinc ions were recovered 58%, 62% of the enzyme activities of heart and 68%, 67% of its activities of placenta by EDTA, respectively. And the inhibition of zinc ion was almostly recovered by adding histone after zinc ion and EDTA preincubation. 3. The inhibition of copper ion was completely recovered by adding of 10 mM of mercaptoetharnol, but zinc ion did not almost affect. 4. The inhibition of zinc ion was completely recovered by adding 50 mM of phosphate, but copper ion did not almost affect. 5. The apparent Km values for S-adenosyl-L-methionine were 4x10^-6 M in heart and 2x10^-6M in placenta. The kinetic analysis of the enzymes from heart and placenta in the presence of 50 uM of copper ion showed that the nature of the inhibition was noncomptitive, considering that Km was constant and Vmax was decreased, and 50 uM zinc ion showed that it was uncompetitive, considering that both Km and Vmax were decreased. These results suggest that the inhibitions of protein methylase II by copper and zinc ion are related to the other mechanisms, respectively.

管群의 流動과 熱傳達에 관한 數値解釋

姜濚珪,朴祥奎 全北大學校 1992 論文集 Vol.34 No.-

The fluid flow and heat transfer in tube bank in crossflow are important in relation to several types of heat exchangers. Numerous experimental studies have been reported previously. Recently, several numerical studies based on the finite difference and the finite element methods have been published. The present study represents the numerical predication of laminar flow and heat transfer in an in-line tube bank at low Reynolds number. The conservation equations of stream function, vorticity and energy were solved numberically using the finite difference method by Gosman et al. Calcurations were carried out for pitch-to-diameter ratios (1.2∼1.5)×(1.2∼1.5), Reynolds number Re = 50∼300, and the Prandtl number Pr=0.7∼15, under the condition of uniform heat flux. This paper presents the characteristic variations of local and mean heat transfer coefficients, pressure drop and friction coefficients, stream-line, isovorticity and isothermal contours, with arrangement, Reynolds and Prandtl numbers.

복막유착의 원인 규명과 예방을 위한 실험적 연구

강대영,송규상,노승무,조준식,이태용 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

Postoperative peritoneal adhesion is the most common cause of intestinal obstruction, and intestinal obstruction is one of the most difficult problems in abdominal surgery. In this experimental study for developing peritoneal adhesion model and its prevention, we divided rats into 5 groups; the talc powder treated group, the cotton-fluff treated group, the talc+fluff treated group, the ischemia group and the control group. There were 16 rats in each group. We made incisions on the midabdomen of the rats and administered talc powder, cotton fluff and talc powder+fluff into the peritoneal cavity respectively. In the ischemia group, we clamped the root of superior mesenteric artery for two minutes to induce ischemia on the small bowel of the rat. We counted the number of the peritoneal adhesive sites in peritoneal cavity on the 7th, 14th , 21st and 42nd postoperative day after peritoneal adhesion induced operation, and observed the characters of the adhesion state. The average number of the peritoneal adhesions were 1.25 per one rat in the talc powder group, 1.44 in the fluff group, 1.31 in the talc+fluff group and 1.31 in the ischemia group compared with 0.94 per one rat in the control group performed sham operation. 91.6% of the all adhesions were observed on the peritoneum of the previous incision sites and only 8.4% of the adhesions were adhered to the peritoneal surface of non-incision sites. The data of this study suggest that peritoneal injury and inflammatory reaction is the major cause of peritoneal adhesion. Also this study indicates that foreign bodies can develop peritoneal adhesion, but the effect is much lower than that of peritoneal injury with inflammatoty reaction.

당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

導入肉牛에 集團發生한 皮膚絲狀菌病(輪癬)에 對하여

康炳奎,魏聖河,朴承柱 全南大學校 農漁村開發硏究所 1984 農業科學技術硏究 Vol.19 No.-

1982年 7月 全南順天地域의 肥育用肉牛에 地域 集團的으로 發生한 皮膚疾患에 대한 炳因學的 調査를 하였던 바, Trichophyton verrucosum에 의한 皮膚絲狀菌炳(輪癬)임을 확인하였다. Bovine ringworm caused by Trichophophyton verrucosum was diagnosed clinically and mycologically. An enzootic of this infection was found in the area of Sunchum district of Korea from Spring to August, 1982.

졸-겔 공정에 의한 실리카-지르코니아 복합 분말 합성

강성,박규섭,배동식 국립7개대학공동논문집간행위원회 2005 공업기술연구 Vol.5 No.-

Silica-zirconia composite nanoparticles have been synthesized by a metal alkoxide hydrolysis and condensation. The size and composition of the synthesized particles can be controlled by manipulating the relative rates of the hydrolysis and condensation reaction of precursor. The average size of range of synthesized silica-zirconia composite nanoparticles was about in the size range of 30-100nm. The effects of synthesis parameters, such as the molar ratio of water to precursor and the molar ratio of water to surfactant, are discussed.

Streptococcus에 대한 chlorhexidine dihydrochloride와 불화나트륨의 효과

강인성,양규호,최남기,김선미,오종석 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.2

Chlorhexidine dihydrochloride와 불화나트륨은 구강 내 세균을 억제하고 치태의 형성을 억제하기 위하여 많이 사용되고 있는 물질이다. 그러나 구강에는 여러 종류의 세균들이 상주하며 이들 물질에 대한 감수성도 다르다. 본 연구에서는 chlorhexidine dihydrochloride와 불화나트륨에 의해 Streptococcus mutans의 인공치태 형성이 억제되었을 때 Streptococcus mutans, Streptococcus sobrinus, Streptococcus oralis, Streptococcus salivarius의 증식에 미치는 이들 물질의 영향을 관찰하여 다음과 같은 결과를 얻었다. Streptococcus mutans를 8시간 배양하면 와이어에 형성된 인공치태 무게는 106.1±18.1㎎이었으나, 1.0μM chlorhexidine dihydrochloride를 첨가하면 5.1±1.5㎎으로 감소되었다(p<0.05). 이 때 배양액의 흡광도도 감소되었다. Streptococcus sobrinus의 배양액 흡광도는 감소되었으나 Streptococcus oralis에서는 감소되지 않았고 Streptococcus salivarius에서는 배양 8시간에 감소되었다가 배양 24시간에 대조군과 차이가 없었다. 배지에 3.0 mM 불화나트륨을 첨가하여 Streptococcus mutans를 8시간 배양하면 와이어에 인공치태 무게는 26.7±8.3㎎으로 감소되었다(p<0.05). 이 때 배양액의 흡광도도 감소되었다. Streptococcus sobrinus와 Streptococcus oralis에서의 배양액의 흡광도도 감소되었으나 Streptococcus salivarius에서는 거의 감소되지 않았다. 이상의 결과는 Streptococcus mutans의 인공치태 형성을 억제하는 chlorhexidine dihydrochloride와 불화나트륨 농도에서 Streptococcus의 여러 종의 감수성이 각각 다르다는 것을 시사하였다. Chlorhexidine dihydrochloride and sodium fluoride have been used as agents inhibiting the replication of oral bacteria and the formation of dental plaque. There are various kinds of bacteria with different sensitivity against these agents. In this study, chlorhexidine dihydrochloride and sodium fluoride were studied about their effects on the replication of Streptococcus mutans, Streptococcus sobrinus, Streptococcus oralis and Streptococcus salivarius at their concentrations inhibiting the formation of artificial plaque by Streptococcus mutans. When Streptococcus mutans was cultured for 8 hours in the media added with l.0μM chlorhexidine dihydrochloride, the weight of formed artificial plaque was decreased to 5.1 ±1.5mg compared with 106.1±18.1mg of the control(p〈0.05). At the same time, the optical density of cultured media was decreased. The optical density of cultured media was slightly decreased in Streptococcus sobrinus. but was not decreased in Streptococcus oralis. The optical density of Streptococcus salivarius was decreased at 8 hours-incubation, was not decreased at 24 hours-incubation. When Streptococcus mutans was cultured for 8 hours in the media added with 3.0 mM sodium fluoride. the weight of formed artificial plaque was decreased to 26.7i8.3 mg(p〈0.05). At the same time. the optical density of cultured media was decreased. The optical density of cultured media was decreased in Streptococcus sobrinus and Streptococcus oralis, but was slightly decreased in Streptococcus salivarius. These results suggest that at the concentration of chlorhexidine dihydrochloride and sodium fluoridehe inhibiting the formation of artificial plaque by Streptococcus mutans, different species of genus Streptococcus show the different sensitivity against these agents.

家兎에 있어서 頭蓋內壓亢進이 Picrotoxin 痙攣에 미치는 영향

姜珍祥,鄭光現,丁相鍵,李順揆 大韓神經精神醫學會 1973 신경정신의학 Vol.12 No.1

After intravenous administration of picrotoxin, 4 steps of responses were observed, i. e. a) tremor and disturbance of righting reflex b) clonic convulsion c) tetanic convulsion and d) death. Picrotoxin (1㎎/㎏) elicited above responses 14.2 (a), 32.6 (b) and 38.8 (c) minutes after intravenous administration, exhibiting shortening of the duration with Increased doses. Jncreased intracranial pressure by intraventricular administration of saline or extradural balloon method facilitated picrotoxin convulsion. Phenobarbital and dilantin suppressed picrotoxin convulsion and facilitating effect of intracranial pressure on picrotoxin convulsion. Possible mechanism of the effect of increased intracranial pressure on picrotoxin convulsion was discussed.

소뇌에 발생한 Nocardiosis : 증례 보고 Case Report

성연상,김상진,박순필,강재규,도종웅 대한신경외과학회 1989 Journal of Korean neurosurgical society Vol.18 No.6

The authors report a extemely rare case of primary cerebellar nocardial abscess. Cerebral nocardiosis is rare disease and often fatal, and often fatal, and commonly spreaded from nocardiosis of lung or skin. Total excision of abscess and medication with sulfonamide is the treatment of choice. The patient was treated by continuous external dirainage with intermittent irrigation of abscess cavity by sulfonamide and given with sulfonamide for 10 months.

삼랑진 지점의 수위-유량곡선식 개발에 관한 연구

강대석,민일규,이승휘,옥치율 東亞大學校 海洋資源硏究所 2003 硏究論文集 Vol.15 No.-

In this study, Development The Rating-Curve Formula through to enforce the discharge investigator on Samrangjin Point. As use the result, We earning rationality, Economical irrigation and establishment a water control project, the basic material of determines hydraulic capacity to design water resources structure. At the same time, We can use The Rating-curve for a high efficient Flood wamning forecast in Nak-Dong River. The result and opinion of the discharge investigator is as follow: 1. The Rating-Curve Formula is Q=(-1065.91941)+1137.237287H+4.671360913H³(1.27<H<9.57) 2. A periodical and continuously management make a routine check over a watermark. 3. When enforce the discharge inverstigator, it require many time, person and equipment. So we can get accuracy and safety working conditions by assist a new system equipment to amount of flowing water measurement. 4. We must sublate to prepare the river bed or collect aggregate as possible, for the upper and downstream section in the established point of a watermark .