Development of a system for S locus haplotyping based on the polymorphic SLL2 gene tightly linked to the locus determining self-incompatibility in radish (Raphanus sativus L.)

Kim, D.,Jung, J.,Choi, Y. O.,Kim, S. Springer Netherlands 2016 Euphytica Vol.209 No.2

<P>To develop a reliable system for identifying multiple S haplotypes controlling self-incompatibility (SI) in radish (Raphanus sativus L.), the genomic organization of the S locus region was identified from radish draft genome sequences. An initial attempt to find the S receptor kinase (SRK) gene, the female determinant of SI, failed due to presence of 15 homologous genes. Using synteny between the radish and Chinese cabbage genomes, the putative S locus region was identified in the radish R7 linkage group. One scaffold anchored to this R7 region contained the S-locus glycoprotein (SLG) gene, which is one of the S locus genes. Using the high homology between the SLG and S domain of SRK, the full-length radish SRK gene containing the largest 6861-bp intron1 was assembled by connecting two scaffolds harboring the S receptor and kinase domains, respectively. A scaffold containing the full-length S-locus cysteine-rich protein (SCR)/S locus protein 11 (SP11) gene, the male-determinant of SI, was identified using information reported previously. Finally, 53,785, 42,804, and 10,165 bp sequences containing the S locus genes and their flanking sequences were obtained. Unlike the various orientations of the SRK or SCR/SP11 genes, the position of SLL2 located at the border region of the S locus was conserved among haplotypes. Sequencing of the SLL2 gene from 31 inbred lines showing differential SI responses revealed 26 polymorphic alleles. Four additional SLL2 alleles were identified from analysis of diverse breeding lines. Based on the polymorphic SLL2 sequences, a new S haplotyping system was developed for efficient marker-assisted selection of the S haplotypes in radish.</P>

d-pinitol regulates Th1/Th2 balance via suppressing Th2 immune response in ovalbumin-induced asthma

Lee, J.S.,Lee, C.M.,Jeong, Y.I.,Jung, I.D.,Kim, B.H.,Seong, E.Y.,Kim, J.I.,Choi, I.W.,Chung, H.Y.,Park, Y.M. North-Holland Pub ; Elsevier Science Ltd 2007 FEBS letters Vol.581 No.1

d-pinitol has been demonstrated to exert insulin-like and anti-inflammatory activities. However, its anti-allergic effect in the Th1/Th2 immune response is poorly understood. Recently, it was shown that T-bet and GATA-3 are master Th1 and Th2 regulatory transcription factors. In this study, we have attempted to determine whether d-pinitol regulates Th1/Th2 cytokine production, T-bet and GATA-3 gene expression in OVA-induced asthma model mice. We also examined to ascertain whether d-pinitol could influence eosinophil peroxidase (EPO) activity. After being sensitized and challenged with ovalbumin (OVA) showed typical asthmatic reactions. These reactions included an increase in the number of eosinophils in bronchoalveolar lavage (BAL) fluid, an increase in inflammatory cell infiltration into the lung tissue around blood vessels and airways, airway luminal narrowing, and the development of airway hyper-responsiveness (AHR). The administration of d-pinitol before the last airway OVA challenge resulted in a significant inhibition of all asthmatic reactions. Accordingly, this study may provide evidence that d-pinitol plays a critical role in the amelioration of the pathogenetic process of asthma in mice. These findings provide new insight into the immunopharmacological role of d-pinitol in terms of its effects in a murine model of asthma, and also broaden current perspectives in our understanding of the immunopharmacological functions of d-pinitol.

Extract of Cassiae Semen and its major compound inhibit S100b-induced TGF-β1 and fibronectin expression in mouse glomerular mesangial cells

Jung, D.H.,Kim, Y.S.,Kim, N.H.,Lee, J.,Jang, D.S.,Kim, J.S. North-Holland ; Elsevier Science Ltd 2010 european journal of pharmacology Vol.641 No.1

Non-enzymatic glycation reactions between reducing sugar and free reactive amino groups of protein lead to the formation of advanced glycation end products, which increase under conditions of aging or diabetes. A previous study showed that extracts of Cassiae Semen (CS), the seed of Cassia tora, had inhibitory activity on advanced glycation end products formation in vitro. To examine the pharmacological effects of a butanol-soluble extract of CS under conditions of diabetic nephropathy, we evaluated the expression of transforming growth factor-β1 (TGF-β1) and fibronectin, key mediators of diabetic nephropathy, in mouse glomerular mesangial cells cultured in the presence of S100b (a specific ligand for receptor of advanced glycation end products). CS inhibited S100b-induced TGF-β1 and fibronectin expression in mouse mesangial cells by suppressing activation of Smad2/3, extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK), and oxidative stress. Moreover, CS suppressed nuclear factor-kappa B (NF-κB) activation in S100b-stimulated mouse mesangial cells. To identify the active compounds of CS, three major compounds, rubrofusarin-6-O-β-d-gentiobioside (CS-A), toralactone-9-O-β-d-gentiobioside (CS-B), and cassiaside (CS-C), were tested in cells. Of these compounds, CS-A significantly decreased the expression of TGF-β1 and fibronectin and NF-κB DNA binding activity. These findings suggest that CS, especially CS-A, has potential as a preventive agent for advanced glycation end products-related diabetic complications.

차체강판 등급에 따른 S-rail 성형성 및 스프링백 특성 비교

정대근(D. G Jung),김흥규(H. K. Kim),김세호(S. H. Kim),김형종(H. J. Kim) 한국소성가공학회 2011 한국소성가공학회 학술대회 논문집 Vol.2011 No.5

In this study, we conducted a series of s-rail forming tests by using sheet sheets with the grades of 340, 440, 590,and 780MPa and then compared formability as well as springback behaviors in the final s-rail products. Various types of drawbeads, i.e. without-bead, locking-bead, and smooth-bead, were applied to the s-rail forming die in order to examine the effect of bead on the s-rail formability and springback. The dimensions of experimentally obtained s-rail products were measured for comparison with finite element predictions. From the comparison, we could confirm that the experimental deformation behaviors agreed well with the finite element predictions in terms of the dependence on sheet sheet grade. The quantitative data of formability and springback for different grades of sheet sheets would be very useful for developing car body parts using high strength steel sheets which are increasingly required by automakers recently.

Valeriana officinalis extract and its main component, valerenic acid, ameliorate d-galactose-induced reductions in memory, cell proliferation, and neuroblast differentiation by reducing corticosterone levels and lipid peroxidation

Nam, S.M.,Choi, J.H.,Yoo, D.Y.,Kim, W.,Jung, H.Y.,Kim, J.W.,Kang, S.Y.,Park, J.,Kim, D.W.,Kim, W.J.,Yoon, Y.S.,Hwang, I.K. Pergamon Press ; Elsevier Science Ltd 2013 Experimental Gerontology Vol.48 No.11

Valeriana officinalis is used in herbal medicine of many cultures as mild sedatives and tranquilizers. In this study, we investigated the effects of extract from valerian root extracts and its major component, valerenic acid on memory function, cell proliferation, neuroblast differentiation, serum corticosterone, and lipid peroxidation in adult and aged mice. For the aging model, d-galactose (100mg/kg) was administered subcutaneously to 6-week-old male mice for 10weeks. At 13weeks of age, valerian root extracts (100mg/kg) or valerenic acid (340μg/kg) was administered orally to control and d-galactose-treated mice for 3weeks. The dosage of valerenic acid (340μg/kg), which is the active ingredient of valerian root extract, was determined by the content of valerenic acid in valerian root extract (3.401+/-0.066mg/g) measured by HPLC. The administration of valerian root extract and valerenic acid significantly improved the preferential exploration of new objects in novel object recognition test and the escape latency, swimming speeds, platform crossings, and spatial preference for the target quadrant in Morris water maze test compared to the d-galactose-treated mice. Cell proliferation and neuroblast differentiation were significantly decreased, while serum corticosterone level and lipid peroxidation in hippocampus were significantly increased in the d-galactose-treated group compared to that in the control group. The administration of valerian root extract significantly ameliorated these changes in the dentate gyrus of both control and d-galactose-treated groups. In addition, valerenic acid also mitigated the d-galactose-induced reduction of these changes. These results indicate that valerian root extract and valerenic acid enhance cognitive function, promote cell proliferation and neuroblast differentiation, and reduce serum corticosterone and lipid peroxidation in aged mice.

자취여대생의 건강증진 생활양식과 관련요인

김다영,김미나,김지혜,김향지,박선아,박성원,정도영,정하나,최소희,이자형,정덕유,오순학 이화여자대학교 간호과학대학 2009 이화간호학회지 Vol.- No.43

The purpose of this study was to know relationship between important variables which influence lifestyle in college women`s health promotion, prove adjustable factors and provide basic data which develops nursing intervention health promotion program. The method of this study was descriptive correlational study. The convenience sample was 206 college woman who live alone in lodgings, dormitory, a reading room or off-campus housing. Research instruments were the Multidimensional Health Locus of Control(MHLC) developed by Wallston, Wallston & Devellis(1978), Interpersonal Support Evaluation List developed by Cohern, Hoberman(1983) and The Health Promotion Lifestyle Profile(HPLP) developed by Walker, Pender & Sechrist(1978) and the data was used after Factor Analysis. The result of this study was follows. 1. It can be found that the level of the college woman`s health promotion lifestyle was 2.46±.33 with higher fulfilling level: According to general characteristic, the level of health promotion lifestyle was 5% significant statistical differences by age and period of menstruation. 2. The level of health locus of control was 3.25±.31 with higher fulfilling level. 3. The interpersonal support level was 3.05±.44 with higher fulfilling level. 4. The level of interpersonal support turned out most convincing factor in health promotion lifestyle (9%). In addition to age factor, two factors was 10% power of explanation in whole health promotion.

셀프리더십에 따른 간호사의 직무만족도

강수연,김수연,도혜진,이혜선,전소현,전혜실,주원진,지윤정,진소희,이자형,정덕유,유소연 이화여자대학교 간호과학대학 2009 이화간호학회지 Vol.- No.43

In this study, we investigated how much job-satisfaction and self leadership nurses have. And making use of self leadership, we hope to enhance job- satisfaction and develop nurse organization. To find out the relationship between nurse's self leadership and Job-satisfaction, we carried out a sample survey : The subjects of our study are 150 nurses being in offices(hospitals) at the two university hospitals and three general hospitals in Seoul or Keong-gi province. Nurses agreed with and understood the purpose of our study. For analyzing the result, we used ANOVA of SPSS 14.0. The main results of our study are as follows : According to characteristic of subjects, level of self leadership has a meaningful difference statistically according to marriage, religion, department career, position. As for the marriage, the level of self leadership of the married are higher((F=19.95, p= .000) than the unmarried. . Level of self leadership perceived by subjects is an average 3.50 point. An average points of each factors are as follows : Self-compensation is 3.75 point, rehearsal is 3.57 point, self-expectation is 3.55 point, goal-setting is 3.53 point, self-criticism is 3.31 point, constructive thinking is 3.27 point. The total average is 62.9 point to 90 full marks. Level of self job-satisfaction perceived by subjects is an average 2.98 point to 5 full marks. Level of self job-satisfaction perceived by subjects is an average 2.98 point to 5 full marks. An average points of each factors are as follows : Interrelation is 3.42 point, interrelation between doctors and nurses is 3.35 point, job demand is 3.10 point, administration is 3.02 point, a professional position is 2.94 point, self control is 2.84 point, payment is 2.44 point. A coefficient of co-relation of self leadership and self job-satisfaction is .406 point, a meaning probability is .000 point and they seem to have a close interrelation. Rehearsal among factors of self leadership has the most interrelation with payment of self job-satisfaction. A professional position of self job-satisfaction has the most interrelation with self expectation(r= .314, p= .000). A job demand has the most interrelation with constructive thinking(r= .272, p= .001). A interaction has the most interrelation with self expectation(r= .272. p= .000). A administration has the most interrelation with constructive thinking(r= .411, p= .000). An interrelation between doctors and nurses has the most interrelation with self expectation(r= .385, p= .000). Our study shows there is a close relation between self leadership and job-satisfaction. Especially, self expectation and constructive thinking, the factor of self leadership, appear to affect the nurse's job-satisfaction. It appears that nurses feel to have a intensive job-satisfaction, having positive self leadership, a sense of purpose, and constructive thought. Thus, we need to steadily study self leadership, self control, and internal motivation as a new paradigm focused on self-control and internal motivation for their self-developments and advancement of nurses' organizations.

Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein‐2 on periodontal regeneration

Song, D‐,S.,Park, J‐,C.,Jung, I‐,H.,Choi, S‐,H.,Cho, K‐,S.,Kim, C‐,K.,Kim, C‐,S. Blackwell Publishing Ltd 2011 Journal of periodontal research Vol.46 No.2

<P> <I>Song D‐S, Park J‐C, Jung I‐H, Choi S‐H, Cho K‐S, Kim C‐K, Kim C‐S. Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein‐2 on periodontal regeneration. J Periodont Res 2011; 46: 193–203. © 2010 John Wiley & Sons A/S</I> </P><P><B>Background and Objective: </B> Recombinant human bone morphogenetic protein‐2 (rhBMP‐2) is a potent inducer for the regeneration of mineralized tissue, but has a limited effect on the regeneration of cementum and periodontal ligament (PDL). The aim of the present study was to determine the effects of rhBMP‐2 on the <I>in vitro</I> and <I>in vivo</I> biologic activity of well‐characterized human PDL stem cells (hPDLSCs) and to elucidate the underlying mechanism of minimal periodontal regeneration by rhBMP‐2.</P><P><B>Material and Methods: </B> hPDLSCs were isolated and cultured, and then transplanted into an ectopic subcutaneous mouse model using a carrier treated either with or without rhBMP‐2. Comprehensive histologic, histometric and immunohistochemical analyses were performed after an 8‐wk healing period. The effects of rhBMP‐2 on the adipogenic and osteogenic/cementogenic differentiation of hPDLSCs were also evaluated. The effect of rhBMP‐2 on both soluble and insoluble collagen synthesis was analyzed, and the expression of mRNA and protein for collagen types I, II, III and V was assessed.</P><P><B>Results: </B> In the present study, rhBMP‐2 promoted both adipogenic and osteogenic/cementogenic differentiation of hPDLSCs <I>in vitro</I>, and the <I>in vivo</I> potential of hPDLSCs to form mineralized cementum and organized PDL tissue was down‐regulated following treatment with rhBMP‐2. Collagen synthesis, which plays a crucial role in the regeneration of cementum and the periodontal attachment, was significantly reduced, with associated modification of the relevant mRNA and protein expression profiles.</P><P><B>Conclusion: </B> In summary, the findings of the present study suggest that enhanced adipogenic differentiation and inhibition of collagen synthesis by hPDLSCs appear to be partly responsible for the minimal effect of rhBMP‐2 on cementum and PDL tissue regeneration by hPDLSCs.</P>

유전질환 등록 시스템 운영, 유전자 정보 Database 구축 및 유전자 진단 시스템 확립

구수경,이환석,손용규,김성수,이광수,김지인,서일선,진수정,정동기,오범석,정성철,이진성 국립보건연구원 2000 국립보건원보 Vol.37 No.-

Arbitrarily Primed PCR을 이용한 한국에서 유행하는 황색포도상구균의 분자유전형에 대한 연구

황선철,이창규,이승관,이동호,정수경,최현일,윤건석,정운원,윤효숙 高麗大學校 倂設 保健大學 保健科學硏究所 1998 保健科學論集 Vol.24 No.1

Eighty methicillin-resistant Staphylococcus aureus(MRSA) isolates were typed by applying arbitrarily primed-PCR(AP-PCR) method to clarify their distribution of molecular genetical characteristics. Among 40 gentamicin resistant strains of MRSA(GR-MRSA), 33 isolates drawn on the dendrogram fell into a single cluster at the similarity level of 90% when primer S₁ was used. However, with the primer S₂, 24 out of 40 strains fell into a single cluster at the similiarity 90%. In the meantime, 22 out of 40 strains amplified fell into a single cluster at the similarity of 90% when the primer E₂ was used. From the combined data obtained, it can be statistically said that 65.8% of GR-MRSA isolates are related with genetical characteristics. In 40 gentamycin susceptible MRSA(GS-MRSA) strains, 18, 19 and 13 strains drawn on the dendrogram fell into a single cluster at a similiarity level of 90% with the primers S₁, S₂ and E₂, respectively. From the combined data obtained by the three above AP-PCR profiles, it can be concluded that 41.7% of GS-MRSA isolates showed high relatedness genetically.