폐암세포에 대한 附子 추출물의 독성 효과

권강범,김은경,문형철,송용선,류도곤 한국전통의학연구소 2006 한국전통의학지 Vol.15 No.1

The aim of this study was to investigate the cytotoxic effect and its mechanism on Radix Aconiti(RA) extract in lung cancer cell lines. RA extract treatment decreased the cell viability in a dose-dependent fashions in lung cancer cells including A549, H460, H23 and H157 cells. Many investigators reported that A549 and H460 cells expressed wild-type p53, but H23 and H157 cells preserved mutated p53. After treatment with RA extract in A549 and H460 cells, we measured the expression of p53 protein levels using Western blot, analysis. In both cells treated with RA extracts, p53 protein expressions were increased in a dose-dependent manner. In our experiments, RA extracts also have cytotoxic effects in H23 and H157, which have mutated p53. Treatment with RA extract decreased bcl-2 protein expressions in both cells. These results suggest that RA extracts have cytotoxic effects via p53 expression increase and bcl-2 inhibitable pathways in A549, H460 cells and H23, H157 cells, respectively.

전경대원에서 발생한 결핵의 임상 양상

문창기,박상준,조민구,김영중,김소연,김윤권,정준오,안석진,김은실,서승오,김지훈,최원제,이윤영,박형기,최규영,김현근 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background: The purpose of this study was to document the incidence and clinical characteristics of patients with tuberculosis (TB) in combat and auxiliary police, living in a group, in Korea where the incidence rate of active TB in a general population is higher than in Western countries. Materials and Methods:We retrospectively reviewed the medical records of all tuberculosis patients diagnosed at National Police Hospital from January 2002 through December 2004. Results:In 2002-2004, a total of 156 cases of tuberculosis were identified with the mean (Standard deviation) age of 20.6 (±1.0) years. Of these, 134 (85.9%) patients were registered as new cases, 11 (7.1%) as relapse, 2 (1.3%) as failure, 5 (3.1%) as treatment-after-default cases whereas 4 (2.6 %) patients were not included in any categories. Average annual new TB rate and smear-positive TB rate were 86.5/10^(5) and 17.4/10^(5) person-years, respectively. In 12 of 31 smear-positive cases, time from onset of symptoms to diagnosis was more than 30 days. Two multidrug-resistance TB cases were identified and two suspected outbreak episodes of TB had occurred during 3 years. Conclusion:There was no statistically significant difference in the incidence rate of new cases of TB between the general population aged 20 to 29 years and combat and auxiliary police in Korea. 배경 : 국내 결핵의 발생은 감소 추세에 있으나 여전히 후진국형 발생양상을 보이고 있다. 특히 집단생활을 하는 젊은이들에서 발생하는 결핵의 유행이 간헐적으로 알려지고 있어 우려를 낳고 있다. 이에 저자들은 최근 전경대원의 결핵 발병 양상에 대해 알아보고자 본 연구를 시행하였다. 재료 및 방법 : 2002년 1월부터 2004년 12월까지 3년간 경찰병원에서 결핵 진료를 받은 전경대원의 의무기록을 후향적으로 분석하여 연도별 결핵 신환발생률, 도말양성결핵 신환율, 진단 지연, 집단발병, 다제내성결핵을 조사하였다. 결과 : 연도별 결핵 신환자 수(10만명당 발생추정치)는 2002년 50명(83.6명), 2003년 42명(83.7명), 2004년 42명(93.5명)이었고 도말양성 폐결핵 신환자 수는 2002년 10명(16.7명), 2003년 11명(21.9명), 2004년 6명(13.4명)이었다. 3년간 도말양성 폐결핵 환자는 31명이 있었고 그 중 12명은 호흡기 증상 발생 30일이 지나서 진단을 받았다. 다제내성 결핵은 2명이 있었으며 결핵의 집단발병이 의심되는 사례는 2차례 있었다. 결론 : 전경대원과 20-29세 연령군 일반인의 결핵 신환발생률, 도말양성 폐결핵 발생률의 통계적으로 유의한 차이는 없었다.

Moon, Yoon-Hee

문윤희,권영창,정인철,이형걸 慶星大學校 1995 論文集 Vol.16 No.2

Frozen beef loin imported from Australia was stored as 2℃ after thawing, then evaluate to improvement of raw meat aroma and palatability of cooked meat by sensory evaluation. To improvement of raw meat aroma and palatability of cooked meat of thawed beef were confirmed for the reason of conditioning after thaw as a result of myofibrillar fragmentation index(MFI) and Mg-ATPase activity of myofibril. Sensory evaluation of raw meat aroma and tenderness on frozen imported beef loin stored at 2℃ after thawing had good in all during cold storage, MFI and Mg-ATPase activity increased, therefore frozen beef loin could recognized to conditioning during stored at 2℃ after thawing. Cold storage after thawing of frozen imported beef loin can improve its cooked aroma and tenderness in case that insufficient conditioning is the reason for its low palatability, and the improvement of palatability on the cooked meat appeared to be greater in aroma than taste. In case of amino acid composition, aspartic acid, threonine, glutamic acid and alanine of imported beef loin stored 7 days after thawing were showed significantly(p<0.05) higher than that stored 0 day after thawing, total amino acid composition of imported beef loin stored 0,3 and 7 days after thawing were 148.40, 171. 06 and 188.99mg/g, respectively.

초음파 펄스에코 데이터 기반 3차원 시각화 방법론

권정현,장홍래,성제중,안형근,장순재,송문호 한국비파괴검사학회 2003 학술대회 논문집 Vol.- No.1

The nondestructive testing using ultrasonic pulse-echo data is an effective test methodology esperially for metal structure. Typically, the ultrasonic pulse-echo data is processed and the results are shown in A-scan, B-scan or C-scan formats. Upon viewing B-scan and C-scan data the viewer is able to identify the location of faults as well as their rough dimension. In this paper, we evaluate the applicability of various 3-D visualization methods frequently used in medical imaging, namely, surface rendering (SR), volume rendering (VR) and maximum intensity projection (MIP), for nondestructive testing purposes. Such 3-D visualization of ultrasonic pulse-echo data enables easier identification of the location and dimension of faults more accurately.

정상 백서 및 담즙성 간경변증을 유발시킨 백서에서 간문맥 결찰시 간장변화에 관한 실험적 연구

채권묵,송훈섭,소병준,문형배 圓光大學校 醫科學硏究所 1996 圓光醫科學 Vol.12 No.2

Portal vein branch ligation has been implicated to stimulate the hypertrophy of the unaffected lobe of the liver, but it has been suggested that the degree of the hypertrophy of the cirrhotic liver would be less than that of the normal liver. So, we tried to induce biliary cirrhotic liver by ligation of the common bile duct for this experiment and to evaluate the difference of the effect of the portal vein ligation among the different experimental rat liver groups. For the experimental study, we used Sprague - Dawley rats which were classified into the two groups. Group A (N = 16): Rats with portal venous branch ligation only. Group B (N=16): Rats with portal venous branch ligation after biliary cirrhosis were made by common bile duct ligation. In order to induce biliary cirrhosis, we ligated the common bile duct ligation in 135 rats and exploratory laparotomy for biopsy was done 3 weeks later after common bile duct ligation and the incidence of biliary cirrhosis was about 40%(54/135). 16 rats with the biliary cirrhotic liver were chosen for further study and one of 5 portal branches was ligated and the rats were sacrificed at different time point(1st, 3rd, 5th, 8th week) after initiation of the experiment, and the livers were removed and examined to find out the gross and histologic changes of the livers. The same procedures were performed in 16 normal rats for control study. In the Group A, the severe inflammatory changes and multiple necrotic foci were shown in the ligated lobe from the 3rd week, and firm nodularity was palpated but hypertrophic changes were not noticed in the unaffected lobe. In the group B, ligated lobe showed the same findings with the group A, but unaffected lobe did not reveal any specific findings. In our experiment, we could induce biliary cirrhosis after common bile duct ligation but we could not examine any hypertrophic changes in the both groups-normal and cirrhotic livers - after portal vein branch ligation. This might suggest that only small portion of necrosis - less than 20% - can not induce compensatory hypertrophy in the unaffected lobe.

中風病辨證診斷標準에 의한 臨床的 硏究

任長信,文祥官,高昌男,權正男,曺基湖,金永錫,裵亨燮,李京燮 경희대학교 1997 慶熙醫學 Vol.13 No.3

원발성 유방암에서 p53 단백발현과 예후와의 상관성에 관한 연구

이학승,이광만,채권묵,문형배 圓光大學校 醫科學硏究所 1995 圓光醫科學 Vol.11 No.1

p53 is a nuclear phosphoprotein which is normally expressed at very low level in all mammalian cells and plays a role in the regulation of cell proliferation. It has also been suggested that normal p53(wild type) serves as a tumor suppressor gene, and inhibits the oncogene-mediated cellular transformation and the rate of cellular proliferation. But, wild type p53 is hardly stained by immunohistochemistry because of its low intracellular concentration and very short half-life. Mutant form of p53 protein is detectable in various human malignancies e.g. colon, stomach, lung and breast cancer by immunohistochemical stain because of its prolonged half-life. In breast cancer, p53 protein expression has been regarded as an unfavorable prognostic factor, but the results of studies about the relationship between the p53 protein expression and prognosis are equivocal. So, author performed this study to evaluate the prognostic significance of p53 expression in 54 patients with primary breast cancer who underwent surgical treatment at Wonkwang University Hospital from October 1985 to September 1991. Follow-up period was 24-91 months (mean: 54.7months). p53 protein was stained by immunohistochemical methods in formalin-fixed, paraffin-embedded tissues using monoclonal antibody(DAKO-p53, DO-7). The prognostic significance of p53 protein was evaluated by positivity and 5-year survival rate, and comparing with well-known prognostic factors of breast cancer. p53 protein was expressed 48.1%(26/54) of primary breast cancers. 5-year survival rate of patients with p53 protein expression was 42.0%, and that of patients without p53 protein expression was 76.4%(p = 0.0277) There was no relationship between p53 protein expression and tumor size, lymph node metastasis, histologic grade or vascular invasion. These results suggest that p53 protein expression is another independent prognostic factor in primary breast cancer.

Development of Protaetia brevitarsis according to the addition ratio of mulberry cake

Moon Hyung Cheol,Ju Rock Lim,Eun Mi Lee,Moon Sik Park,Sun Young So,Hyung Kwon Cheon 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.04

This study was conducted to evaluate the the efficacy of mulberry cake mixed diet on larval growth of Protaetia brevitarsis. As a result of adding 3, 5, and 10% mulberry cake to the fermented mulberry sawdust, the development period of the Protaetia brevitarsis larvae was 44.9days, 44.6days, and 41.8days, and the highest weight of larvae was 2.76g, 2.80g, and 2.89g, respectively. As a result of adding 3, 5, and 10% mulberry cake to the fermented oak sawdust, the development period of the Protaetia brevitarsis larvae was 46.7days, 41.6days, and 41.7days, and the highest weight of larvae was 2.65g, 2.65g, and 2.56g, respectively.

Generation of 1D4 Single Chain Fv-Fc

Hyung-Geun Song, Kwon Pyo Hong, Yoo Ri Moon, Hyeong Jin Ji, Byeongwoo Ahn 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.3

Immunotherapeutic approaches using agonist antibodies or fusion proteins of immunomodulatory molecules significantly inhibit tumor growth and boost cell-mediated immunity. We isolated mRNA from previously reported 1D4 hybridoma cells and amplified the variable regions of the heavy chain (VH) and light chain (VL) genes using reverse-transcriptase polymerase chain reaction. Using a linker, the amplified sequences for the heavy and light chains were each connected to the sequence for a single polypeptide chain that was designed to be expressed. VL and VH fragments were cloned into the pOptiVEC-TOPO vector containing the human CH2-CH3 fragment. Then, 293T cells were transfected with the 1D4 single-chain Fv-Fc (scFv-Fc) constructs. A549 cells were used for presentation of the 1D4 antigen. Flow cytometry was performed for analysis of the secreted 1D4 scFv-Fc constructs. The DNA sequence of 1D4 scFv-Fc was obtained. The 1D4 scFv-Fc constructs were expressed by the transfected 293T cells and secreted into the culture medium. The immunoreactivity of the secreted scFv-Fc construct was lower than that of the murine 1D4 antibody for A549 cells. A 1D4 scFv-Fc construct for immunotherapy was developed.

Design, Synthesis, and In Vitro Evaluation of APIO Analogue of Neplanocin A

Moon, Hyung Ryong,Kwon, Sung Hee,Lee, Jeong Ah,Yoo, Byul Nae,Kim, Hea Ok,Chun, Moon Woo,Kim, Hee-Doo,Kim, Joong Hyup,Jeong, Lak Shin 梨花女子大學校 藥學硏究所 2003 藥學硏究論文集 Vol.- No.12

A novel apio analogue of neplanocin A was efficiently synthesized from D-ribose via stereoselective aldol-retroaldol reaction for introducing hydroxymethyl group and RCM reaction for synthesizing carbocycle, and its inhibitory activity against SAH hydrolase was assayed.