Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

Pre-Stress 狀態에서 一定한 反復變位에 依한 砂岩의 疲勞破壤擧動에 關한 硏究<第2報> : 力學的係數들과 彈性度 및 塑性度의 變化 Variations Mechanical Moduli, Elastic and Plastic Degree

金溢中,金昇坤,金永錫 全北大學校 1989 論文集 Vol.31 No.-

The uniaxial cyclic displacement tests were performed to investigate and examine the fatigue failure behavior of sandstone at the staged pre-stress levels and under constant-displacement boundary conditions. The experimental results reported here have been limited to variations of elastic moduli, elastic degree and plastic degree with the staged pre-stress levels and the number of cycles. Some of the conclusions which may be derived from the results of the studies are as follows : 1. When the rocks failed, the elastic moduli and the elastic degree rapidly decreased, but the plastic degree rapidly increased. The higher the rocks were loaded in comparison with previous ones, the higher the plastic degree, and the shorter fatigue life. 2. In a second run after recess the displacement, chord modulus was also rapidly increased at the second cycle, because a large quantity of plastic strain was produced at the first cycle. 3. The fatigue life must be relatively determined not only with respect to the decreasing rate of compression modulus and dynamic compression modulus, but also the plastic degree and plastic strains. 4. The elastic degree was not proportional to the elastic strains, while the plastic degree was proportional to the plastic strains 5. As an increasing rate of strain decreases, recovery modulus and chord modulus curves approach each other, and their separation means that fatigue limits have been reached.

한천, Sodium Alginate 및 Carrageenan첨가가 유과(부수게)바탕의 품질에 미치는 영향

김중만,전예정,박효숙,송영애,백승화,김명곤 한국식생활문화학회 2005 韓國食生活文化學會誌 Vol.20 No.1

This study was to evaluate effects of agar, sodium alginate and carrageenan on quality of Yugwa(Busuge) base. In the base preparation agar, sodium alginate and carrageenan were added 0.0, 0.1, 0.5, 1.0 and 3.0%(w/w), respectively. Volume, shape, hardness. color(L, a and b value), crude lipid content and sensory evaluation(taste and crispness) of the Yugwa base was measured. Volume of the base was higher than control in case of 0.1~0.5%(w/w) agar, sodium alginate and carrageenan, of which sodium alginate was the highest. Shapes were similar to control. Hardness and crude lipid content was decreased proportional to amount of addition of the three seesweed polysaccharides. the whiteness(L-value) was increased but the yellowness(a-value) and the redness(bvalue) decreased. Taste and crispness were increased in the case of 0.1~1.0%(w/w) of sodium alginate, but agar and carrageenan decreased.

수술전 임상등급이 좋은 뇌동맥류의 치료결과와 나쁜 예후의 원인

김창희,허승곤,정남,신문수,이광수 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.4

We analyzed the overall surgical results in 100 patients with intracranial aneurysms operated in Presbyterian. Medical Center during the period from June 1990 to December 1993. Preoperatively, all patients were in Hunt and Hess grades Ⅰ-Ⅲ. Overall outcomes revealed 83 patients(83%) of good outcome. 10 patients(10%) of fair outcome. 3 patients(3%) of poor outcoms. and 4 patients(4%) of dead outcome. The outcome was worse if surgery was performed in the 4 to 10 day post-bleed interval(P<0.05) and there was no statistical difference of the rate of good outcome between the patients operated at 0 to 3 days. and 11 day and more after subarachnoid hemorrhage. In good grade patients. surgical trauma rather than delayed ischemic deficit was considered as the major cause of unfavorable outcome of aneurysm surgery Intermediate period operation increased the chance of postoperative do-layed ischemic deficit Systemic complications and vasospasm were 4he major causes of dead outcome in the oldaged patients.

액체 ^⁴He에서의 μ^- 및 π^- X-선 강도 계산

김승곤 全北大學校 敎育大學院 1984 敎育論叢 Vol.4 No.-

Cascade calculations have been performed in an effort to better fit measured absolute yields of muonic and pionic X-rays in liquid ^4He. With the bell-shaped intial distribution calculated by Haff and Tombrello a slight improvement over the last calculation was made in agreement with the measured mesic X-ray yields.

노랑초파리에 대한 Esterase Isozyme의 변이에 관한 연구

김승곤 최신의학사 1984 最新醫學 Vol.27 No.9

1983년 7월부터 10월사이에 노랑초파리(D. melanogaster)를 3개 자연집단 부천, 전주, 울산에서 채집한 것을 사용하여 한천 Gel 전기영동법으로 Esterase Isozyme을 분석하였다. Est-C는 3개(C_(O), C^(S), C^(F))의 복대립유전자로 분리되었고, Est-6도 3개(6^(O), 6^(S), 6^(F))의 복대립유전자를 포함하고 있었다. Est-C lows의 빈도는 지역적으로 차이는 있으나 어느 지역이던지 Est-CF가 0.9616이었고 Est-6^(S)가 0.9396로 가장 높게 분석되었다. Est-C와 Est-6의 연쇄형은 전주가 95% 울산이 90% 이상씩 연쇄되어 있었다. Esterase isozyme was analysed by the method of agar-gel electrophoresis in the flies collected from three localities, Bucheon, Cheonju and Ulsan. Est-C was devided in 3 multiallele (C^(O), C^(S) and C^(F)), and the patterns of Est-6 was also isolated 3 allele (6^(O), 6^(S) and 6^(F)). The frequency of Est-C locus was some different in 3localities, and the high frequencies of Est-C^(F) (0.96) and Est-6^(S) (0.94) was detected compared to the other locus.

겔화 반응 및 열처리한 금속염화물의 결정 및 구조특성

김인태,박환서,유승곤,김환영,김준형 한국공업화학회 2003 응용화학 Vol.7 No.2

A new concept for the stabilization and solidification of volatile radioactive wastes via gelation in a simple material system is introduced in this paper. Using sodium silicate as a reaction module and phosphoric acid as a catalyst or stabilization agent. CsCl is chemically converted to thermally stable compounds. The heat-treated product at 1000℃ contained a cristobalite crystalline framework and a glassy phosphorus-rich phase with a high Cs content. The morphology looked like honey flowing on the honeycomb. From TG/DT analysis, Cs could not be vaporized below 1300℃ by changing the aging conditions and molar ratio of Si/p. As a new concept, GRSS(gel-route stabilization and solidification) could give us the chance to make the high temperature waste conditioning process more stable and economical.

복합 관동맥병변에서의 Rotational Atherectomy : 초기결과

김진우,박성욱,홍명기,이상곤,이일수,이철환,최기준,강덕현,송재관,김재중,박승정 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.9

濟州道 中學校 體育科 敎科 指導 및 評價 實態에 關한 調査 硏究

김승곤,강승주 濟州大學敎 體育科學硏究所 1997 체육과학연구 Vol.3 No.-

A questionnaire was given to 92 teachers of physical education in middle school Cheju Do on their teaching method and evaluation conditions. And, the result is obtained as follows. 1. Teachers of physical education have a good understanding of the ultimate aim of physical education. In addition, students are taught to promote their physical strength and to continue to be trained in all their life. 2. In the priority order of teaching ball games, the first is soccer. the second is volleyball and the third is basketball in the case of boys. On the other hands, the priority order is volleyball, table tennis, badminton and basketball in the case of girls. That is w want to play these sports and continue them in all their lives. 3. Training. sports events and official trips of physical teachers cause loss of gymnastics classes in school. But they try to reduce them through shifts of lesson and supplementary lectures. 4. In teaching of domain of recognition. teachers instruct general theory including the text book of physical education. 5. One of public facilities that teachers need very much is Gymnasium. 6. Athletics skill test is clearly fair because test is based on standard provided by Ministry of Education. Teachers give skill test items to students and provide opportunities to practice for themselves.

UO_2 핵연료봉에 대한 감마선의 투과율 측정

金承坤,姜正求,李延玉 全北大學校 1993 論文集 Vol.36 No.-

A depleted uranium dioxide pellet(φ=12.1nun, l=4.4mm) irradiated in KAERI TRIGA Mark-Ⅲ Reactor was measured with a HPGe detector to determine the product of transmission factor of gamma rays within the source itself and the detector efficiency as a function of energy using gamma rays emitted by fission products ^140L_a, and ^144P_r. The results appear markedly different from the detector efficiency, and the reasons for the deviation were contemplated by extracting a transmission curve as a function of gamma ray energy.