Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

Kim, Hyung-Keun,Song, Mina,Jun, ji-Hae,Woo, Kyung-Mi,Kim, Gwan-Shik,Baek, Jeong-Hwa The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone merabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and 1,25(OH)_(2)vitaminD_(3)(VD3) by using mouse oligo 11 K gene chip. In the presence of 10 mM[Ca^(2+)]e or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expressions of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high ectracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated;slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated;s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracelluar calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.

Berberine의 항생제 내성 황색포도구균에 대한 항미생물 효과

김해경,차정단,유용욱,김강주,이동근 원광대학교 생체재료·매식연구소 2000 원광생체재료·매식 Vol.9 No.1

Antibiotic-resistant Staphylococcus aureus (S. aureus) emerged in many parts of the world and increased complex clinical problems. Therefore, new agents are needed to treat to antibiotic-resistant S. aureus. Some natural products are candidates of new antibotic substances. Previous reports showed that the extract of Coptidis Rhizoma and Phellodendri Cortex have antimicrobial effects. The main antibacterial substance of Coptidis Rhizoma and Phellodendri Cortex is berberine. In this study, we examined the antimicrobial activity of berberine to antibiotic-resistant S. aureus. We isolated the 13 strains of antibiotic-resistant S. aureus from the oral cavity of healthy children in a preschool, and investigated the antimicrobial activity of berberine. We examined the effects of berberine on the level of resistance to antibiotic-resistant S. aureus. We observed that the invasion of S. aureus into human gingival fibroblasts was blocked by berberine. Berberine inhibited the growth of antibiotic-resistant. S. aureus as a dose dependent maner. Minimum inhibition concentration (MIC) of berberine against antibiotic-resistant S. aureus was 50-100 ㎍/ml. The inhibition of S. aureus invasion was depended on the concentration of berberine in human gingival fibroblasts. The MIC of ampicillin against antibiotic-resistant S. aureus was 32 ㎍/ml. However, the MIC of ampicillin against antibiotic-resitant S. aureus in the presence of 50 ㎍/ml berberine was 1 ㎍/ml. These data indicate that the combination with berberine and β-lactam antibiotics resulted in the synergistic reaction. These results suggest that the berberine may have the antimicrobial activities to antibiotic-resistant. S. aureus, and the mechanism may be the inhibition of growth and intracellular invasion. and alteration of antibiotic-resistance level of antibiotic-resistant S. aureus.

진동공구 사용에 의한 Hand Arm Vibration Syndrome 1례

김수근,정해관,황정수 東國大學校醫學硏究所 2002 東國醫學 Vol.9 No.1

수완진동증후군은 진동공구를 사용하는 작업자에게 발생한다. 이 질병은 레이노 현상이 직업성 원인으로 오는 경우로 1950년대에는 진동증후군 또는 vibration induced white finger(VWF)로 알려졌고, 1983년 영국 런던국제회의에서 국소진동에 의한 건강장해에 대항 병명을 통일하여 "수완진동증후군(hand-arm vibration syndrome)"이라고 정하였다. 이것은 한랭노출시 수지색깔이 희게 변하고 손과 팔에 통증이 나타나며, 골관절염이 발생하게 된다.자동차 정비 작업장에서 11년간 임팩터 등 진동공구를 사용한 근로자가 한랭노출시 수지 색깔 변화와 저린감 등을 호소하여 이에 대한 의학적 면담과 객관적 소견을 얻기 위한 한랭유발반응검사, 진동감각역치 검사와 상지혈관조영술 및 감별진단을 위한 항핵항체와 한랭면역글로블린 검사를 실시하여 수완진동증후군으로 진단하였다. A hand-arm vibration syndrome occurs in some workers who use hand held vibration tools. It is known by Raynaud's phenomenon of occupational origin in 1950s as 'the vibration syndrome' or vibration induced white finger' has, in the 1980s, been renamed 'the hand arm vibration syndrome'. It consist of white fingers, pain in the hand and arm, and a small excess risk of osteoarthritis. We report a case of hand arm vibration syndrome in worker exposed to hand arm vibration diagnosed objectively by skin emperature, vibration sens changes and upper extremity angiogrphy.

참박과 호박 종자의 발아촉진을 위한 저선량 감마선의 효과

김재성,백명화,이영근,이혜연,유준철 한국환경농학회 2002 한국환경농학회지 Vol.21 No.3

참박과 호박 채종종자의 발아율을 증진시킬 수 잇는 종자 처리 방법을 확립하고자 저선량 감마선을 1∼20 Gy 까지 조사하여 발아와 초기생육 및 생리활성을 관찰하였다. 저선량 조사한 참박과 호박종자의 발아율은 대조구보다 증가하는 경향을 보였는데 효과는 종자상태에 따라 다르게 나타났다. 참박 70∼80% 발아율종자의 발아는 4∼20 Gy에서 증가하였는데 특히 4 Gy조사에서 가장 높았고, 80∼90% 발아율종자도 4 Gy 조사에서 효과를 보였다. 파종 4일 후의 발아율은 참박 70∼80%와 80∼90% 발아율 종자의 경우는 4 Gy조사에서 대조구보다 각각 66%와 26% 증가하였고, 호박의 70∼80% 발아율종자는 2 Gy 조사에서, 80∼90% 발아율종자는 8 Gy 조사에서 향상되었다. 저선량 조사한 유묘생육은 발아율이 증가한 선량에서 증가하였는데 특히 호박 70∼80% 종자의 2 Gy 조사에서 가장 높았다. 파종 6일 후에 조사한 참박의 효소활성은 4 Gy조사시에 단백질과 catalase 활성이, 호박의 경우는 catalase와 peroxidase 활성이 2 Gy와 8 Gy 조사에서 증가하였다. 참박과 호박종자의 발아와 유묘생육 및 생리활성은 저선량 감마선 조사에 의해 촉진됨을 확인할 수 있었다. To establish the seed treatment method of the gamma radiation for germination enhancement of bottle gourd(Lagenaria siceraria Standl) and pumpkin(Cucurbita ficifolia Bouche), seeds were irradiated with the dose 1~20 Gy of the gamma radiation. The germination rate in irradiated group was higher than that of the control, depending on seed condition. In the case of bottle gourd, the germination rate of 70~80% germinative seed was increased at 4-20 Gy irradiated groups. Especially, it was the highest at 4 Gy irradiated group. The germination rate of 80~90% germinative seed was the highest at 4 Gy irradiated group. The germination rate of 70~80% and 80~90% germinative seed at 4 days after sowing was increased 66% and 26% at 4 Gy irradiated group, respectively. In the case of pumpkin, the germination rate of 70~80% and 80~90% germinative seed was increased at 2 Gy and 8 Gy irradiated group, respectively. Effect of the low-dose gamma radiation on the early growth was enhanced depending on their germination enhancement. Especially, it was the highest at 2 Gy irradiated group of 70~80% gen inative seed of pumpkin. Catalase activity of bottle gourd was increased at 4 Gy irradiated group. Catalase and peroxidase activity of pumpkin were increased at 2 Gy and 8 Gy irradiated group. These results suggest that the germination, early growth and physiological activity of bottle gourd and pumpkin seeds could be promoted by the low dose gamma radiation.

N-Oxyldiethylenebenzothiazole-2-sulfenamide의-S-N= 결합에 대한 전기화학적 환원

金海珍,鄭根鎬,崔圭源,金日光,林善英 원광대학교기초자연과학연구소 1992 基礎科學硏究誌 Vol.11 No.3

N-Oxyldiethylenebenzothiazole-2-sulfenamide(ODBS ; 가황촉진제)의 전기화학적 환원을 직류와 시차펄스 폴라로그래피 순환 전압전류법, 조절전위 전기량법으로 연구하였다. ODBS의 전극환원과정은 단일 환원파(-1.86 volts vs. Ag/0.1M AgNO₃ in AN)에서 비가역으로 3전자가 이동하는 E-C-E-C 반응메카니즘으로 진행되었다. 조절전위 전기분해 결과 sulfenamide(-S-N=) 결합이 끊어지고 mercaptobenzothiazole(MBT)과 benzothiazole disulfide(MBT dimer) 그리고 유리된 sulfur 등이 생성물로 얻어졌다. pH 변화에 따른 폴라로그램의 해석과 생성물 분석의 결과를 바탕으로하여 전기화학적 반응 메카니즘을 제안하였다. The electrochemical reduction of N-oxyldiethylenebenzothiazole-2-sulfenamide(ODBS; vulcanization accelerator) was investigated by direct current polarography, differential pulse polarography, cyclic voltammetry and controlled potential coulometry. The irreversible electrode reduction of ODBS proceeded E-C-E-C reaction mechanism by three electrons transfer with irreversible one wave(-1.86 volts vs. Ag/0.1M AgNO₃ in AN). As the results of controlled potential electrolysis, mercaptobenzothiazole(MBT), benzothiazole disulfide(MBT dimer) and extricated sulfur were products which followed by cleavage of the sulfenamide(-S-N=) bond. Upon the basis of products analysis and polarogram interpretation with pH variable, electrochemical reaction mechanism was suggested.

Berberine의 항생제 내성 확색포도구균에 대한 항미생물 효과

김강주,차정단,김해경,유현희,유용욱,이동근 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.2

Antibiotic-resistant Staphylococcus aureus (S. aureus) emerged in many parts of the world and increased complex clinical problems. Therefore, new agents are needed to treat the antibiotic-resistant S. aureus. Some natural products are candidates of new antibiotic substances. Previous reports showed that the extract of Coptidis Rhizoma and Phellodendri Cortex have antimicrobial effects. The main antibacterial substance of Coptidis Rhizoma and Phellodendri Cortex is berberine. In this study, we examined the antimicrobial activity of berberine to antibiotic-resistant S. aureus. We isolated the 13 strains of antibiotic-resistant S. aureus from the oral cavity of healthy children in a preschool, and investigated the antimicrobial activity of berberine. We examined the effects of berberine on the level of resistance to antibiotic-resistant S. aureus. We observed that the invasion of S. aureus into human gingival fibroblasts was blocked by berberine. Berberine inhibited the growth of antibiotic-resistant S. aureus as a dosw dependent maner. Minimum inhibitory concentration (MIC) of berberine against antibiotic-resistant S. aureus was 50∼100㎍/㎖. The inhibition of S. aureus invasion was depended on the concentration of berberine in human gingival fibroblasts. The MIC of ampicillin against antibiotic-resistant S. aureus was 32㎍/㎖. However, the MIC of ampicillin against antibiotic-resistant S. aureus in the presence of 50㎍/㎖ berberine was 1㎍/㎖. These data indicate that the combination with berberine and β-lactam antibiotics resulted in the synergistic reaction. These results suggest that the berberine may have the antimicrobial activities to antibiotic-resistnat S. aureus, and the mechanism may be the inhibition of growth and intracellular invasion, and alteration of antibiotic-resistance level of antibiotic-resistant S. aureus.

마산연안지역의 국지풍 순환 및 대기오염물질 확산에 관한 연구

김유근,이화운,문윤섭,김해동,원경미,정우식,오인보 부산대학교 환경문제연구소 1997 環境硏究報 Vol.15 No.1

To investigate a horizontal windrose and the mixing height in Masan coastal area, a local atmospheric flow and the concentration of air pollutants were analyzed by abserved data from AWS, airsonde and air sampler. The land-sea breeze model and the ISCST2 model of U. S. EPA(Environmental Protection Agency) were used to predict the concentration of air pollutants such as SO2 and TSP. As a result, during spring the principal wind direction in the Masan region is mainly represented by windrose of the WNW and NW, which can be induced by land breeze and the SE and SSW of sea breeze even if weak wind speed. The mixing height during the measurement was marked with the range from 400 m(0900 LST) to 1450 m(1500 LST), and the height of an inverse layer was presented as the range from 30 m(2100 LST) to 150 m(0300 LST). It is very important to cosider the local wind field for the accurate diffusion and movement of air pollutants in coastal urban area. The atmospheric flow was simulated by the two-dimensional local wind model upon considering the orographic characteristics such as land-sea contrast, mountain valley and urban building. The result of air quality simulated by ISCST2 support that the dispersion pattern of the pollutants in that area was followed as local circulation system in coastal urban area.

뉴로-퍼지 논리를 이용한 12경락의 양도락진단 시스템의 구현

김병화,한권상,이우철,김성현,최해성,안현식,김도현 대한전자공학회 1997 秋季綜合學術大會 論文集 Vol.20 No.2

실리콘 웨이퍼 태양전지의 효율 향상 방안

김정근,박해웅,장영철 한국기술교육대학교 2006 論文集 Vol.12 No.1

In the process of ingot pulling of the Si single crystal, the pulling velocity of the single crystal silicon should be faster than the dislocation propagation velocity in order to have uniform resistivity in (100) plane. The (100) plane has no dislocation when single crystal seed is contacted and seperated from the melt. Oxygen content of the Si ingot should be controlled by melting Si in Ar gas atmosphere with magnetic field to increase the viscosity of the melt. The efficiency of the Si wafer solar cell can be enhanced by (1) removing the defects in the Si wafer, (2) minimizing the recombination and thermalization of the atoms which lead to energy loss, (3) forming pyramid structure on the cell surface to reduce reflection of the light. The shading of the cell can be minimized via controlling of the finger width of the solar cell. the atoms which lead to energy loss, (3) forming pyramid structure on the cell surface to reduce reflection of the light. The shading of the cell can be minimized via controlling of the finger width of the solar cell.

전위된 상악 견치 및 하악 측절치의 치험례

권해숙,현홍근,김영재,김정욱,장기택,김종철,한세현,이상훈 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.4

Tooth transposition is an anomaly of eruption characterized by the interchanged positions of two adjacent teeth, which is divided into complete and incomplete transposition. There are three common approaches for treating transposition: aligning the involved teeth in their transposed positions, moving them to their correct anatomic position in the arch and extracting one of the transposed teeth. Considerations in treatment plans are esthetic, function, risk of jeopardizing the roots and damaging the supporting structures, position of the root apex, developmental stages of teeth and expected compliance. The presented case reports described one maxillary canine-the first premolar transposition and two mandibular lateral incisor-canine transpositions. The former transposed teeth were arranged in their transposed position, and the latter transposed teeth were rearranged into their normal position. 치아전위란 인접한 두 치아의 위치가 서로 바뀐 치아 위치 이상을 말하며, 치관과 치근의 위치에 따라 완전전위와 불완전전위로 나눌 수 있다. 하악보다는 상악에 편측성으로 호발하며, 특히 상악 견치에서 자주 발생한다. 전위는 발견 시기와 전위된 양상에 따라 치료방법이 달라질 수 있다. 본 증례는 상악 견치와 제1소구치 간의 전위가 일어난 10세 7개월의 환아와 하악 측절치와 견치 간의 전위가 일어난 8세 11개월 환아와 8세 9개월 환아의 교정치료에 대한 것으로 양호한 치료 결과를 얻었고, 증례 별로 치료방법의 차이점에 관한 고찰을 하였기에 이를 보고하는 바이다.