肺炎樣疾患의 臨床的 觀察

김기호,홍순조,이효식,강문원,김호연,정규원,정희영,전종휘,김선무,이종무,김정진 대한의학협회 1976 대한의학협회지 Vol.19 No.4

Propylthiouracil에 의한 중증 급성간염 1예

임종주,심미령,이명수,김태현,오석규,안선호,박무림,김남호,박병현,조성구 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.2

PTU에 의한 간염의 발생은 매우 드물게 발생하는 것으로 알려져 있으나 저자들은 Graves씨 병으로 진단 받고 propylthiouracil 투여를 받고있던 환자에서 중증의 급성 간염이 발생한 1예를 경험하였기에 보고하는 바이다. Propylthiouracil is widely used to treat patients with hyperthyroidism. This drug has been associated with severe hepatotoxicity rarely. We presented the case of jaundice and profound liver dysfunction from a 23-year old woman treated with propylthiouracil for hyperthyroidism. Viral, metabolic, and autoimmune liver disease could be excluded and liver biopsy revealed a pattern of acute hepatitis. After discontinuing the drug, there was a progressive resolution of hepatic symptoms and decrease in biochemical data of the liver. Despite propylthiouracil induced hepatitis in rare case, patients receiving propylthiouracil are exposed to develope severe hepatotoxicity. Therefore it might be advisable to monitor level of the transaminase on regular base from patients receiving propylthiouracil.

감나무탄저병에서 분리한 Gloeosporium kaki의 2계통

崔貞植,李貴宰,金炯武,蘇仁永 全北大學校 1993 論文集 Vol.35 No.-

A anthracnose symptom at persimmon tree was found out in the field, Chonju in Chonbuk province and Haenam Experiment Station in Chonnam province in 1991 and 1992. The cultivated character and the pathogenicity of the fungi were as follows : These fungi, Gloeosparium kaki which were isolated from the anthracnose were classified into strain Ⅰ and Ⅱ by the cultivated characteristics. The size of conidia in strain Ⅰ was 22.5 × 6.82 ㎛ and that of strain Ⅱ was 20.3 × 6.2 ㎛. In a PDA, the colour of strain I was light brown and made a central zone. The colour of strain Ⅱ was black brown and deep grey and didn't make a central zone. The pathogenicity of strain Ⅰ was similar to strain Ⅱ in the leaf, branches.

한국산야생등줄쥐(Apodemus agrarius coreae)의 해마복합체에서 neuropeptide Y 면역반응세포에 관한 연구

김무강,송치원,이경열,박일권,정영길,이강이,김종섭 충남대학교 수의과대학 동물의과학연구소 1997 動物醫科學硏究誌 Vol.5 No.-

Neuropeptide Y(NPY) was first isolated from porcine brain. This discovery has lead some workers to study the distribution of this peptide in the central nervous system of various mammals. In this study the distribution pattern of neuropeptide Y-immunoreactive neurons was examined in the hippocampal complex of Striped field mouse in Korea(Apodemus agrarius coreae) Experimental animals were anesthesized with thiopental sodium and perfused with 4% paraformaldehyde through the left ventricle and the ascending aorta. The brains were removed and transferred 10%, 20% and 30% sucrose solution. Sections were cut on a cryostat into 40㎛-thick. The tissue was immunostained with free-floating and avidin-biotm complex(ABC) method. The results obtained in this study were summarized as followings. 1. The most of NPY-IR neurons in hippocampus proper were found in the polymorphic layer and a few was found in the pyramidal layer. 2. The process run vertically in the deep polymorphic layer and run parallel to corpus callosum in superficial polymorphic layer. 3. The shapes of neurons in hippocampus proper were varied of polymorphic, oval or round, and the nerve fibers were found in the molecular laver and the hippocampal fissure. 4. The highest density in number of NPY-IR neurons on dentate gyrus were found in hilar area of dentate gyrus. 5. The most of NPY-IR nerve fibers were found in molecular cell layer.

중등체육교육과정 변경에 따른 무용 교육 내용의 변천 고찰

정청자,이무영 江原大學校附設 體育科學硏究所 1992 江原大學校附設體育科學硏究所論文集 Vol.- No.17

The curriculum of the secondary physical education in Korea has been reformed four times, in 1963, 1973, 1981 and 1987 respectively, ever since it was first established in 1955 by The Ministry of Education. And, the dance education program, being merely a part of the physical education in the course throughout the period, has been naturally changed accompanied by the reorientation of the curriculum. In the initial stage, 1955∼1963, the dance education was treated as insignificant and all they could do in the class was to cram the existing works because the first curriculum established in 1955 attached stress mostly on health, hygiene and physical strength. From 1963 to 1973, the second stage, the education for the whole man was particularly emphasized, and in dance education, thereafter, stress was placed on cultivation of the creativity and the power of expression that can help oneself to express ones body motion. The distinctive feature for the third stage, 1973∼1981, was that the program became active since it was settled to form 15% of the course. And the program was classified into theory of the dances, creative dances, Korean folk dances and foreign folk dances. The main point of the program in the fourth stage, 1981∼1987 was substantially the same as that of the third stage wish minor difference in that the foreign folk cances were assigned into three different school grades so as to make the student familiar stepwise with various cultures and customs from all parts of the world. In the last stage, from 1987 to date, the great emphasis was placed on the original dance in which student's originality can be exercised through the creative action expressing inner world of human nature by means of body motion. Consequently, this study apparently indicates that the secondary dance education program has been ceaselessly progressed even though the pace has not been agreeable. It should be urged, however, to reconsider and improve in that 1) a given condition for the dance education program such as class hours, teachers and familities in charges fall far behind the other part of the art course, music and fine arts: 2) this program is restricted only for the girls, and 3) this program is not yet liberated from the physical education.

영지버섯 栽培에 있어서 푸른곰팡이 병균의 生長에 미치는 要因과 防除法

李鍾縕,張茂雄,李在成 嶺南大學校附設 基礎科學硏究所 1986 基礎科學硏究 Vol.6 No.-

本 實驗은 우리나라의 霙芝 버섯의 포트 栽培에서 發生하는 푸른곰팡이병의 原因茵의 규명과 그 防除法을 확립하고져 실시하였다. 實驗結果는 곰팡이 3種과 細茵 3種의 分離되었다. 이중 푸른곰팡이 症狀의 原因茵은 Trichoderma Roningi, T. lignorum, 未同定 1種임이 확인되었고, 발생빈도는 각각 60%, 30%, 10%이었다. T. lignorum은PDA 배지 Waksman's and Richard's solution에서 생장이 좋았고, 중성-알카리성에서는 生長이 不良한 반면 산성에서 生長이 좋았으며, 최적산도는 pH4였다. Trichoderma spp. 는 70℃에서 60分間, 80℃에서 30分間 열처리하므로서 완전히 사멸되었다. Three species of Trichoderma causing green mildew of Ganoderma lucidum were isolated from 6 spots of G. lucidum growing areas in Dae Gu. These are T. roningi, T. lignorum, and an unidentified species, and their frequencies of occurrence are 60%, 30%, and 10%, respectively. All of these species grew well in potato dextrose, Waksman's and Richard's solution, and preferred acidec(pH4.0) to neutral condditions. T. lignorum was killed when exposed for either 60minutes at 70℃, or for 30minutes at 80℃.

대조 추출물분획이 치은 섬유아세포의 생물학적 활성화에 미치는 영향

양창호,이용무,조기영,배기환,정종평 충남대학교 약학대학 의약품개발연구소 1994 藥學論文集 Vol.10 No.-

Final goal of periodontal treatment is to reconstruct the destructed periodontal tissue as well as to remove the necrotic pathologic elements. The purpose of this study is to investigate on the effect of Zizyphi extract to the inhibitory ability on collagenolytic activity of P gingivalis, biologic activity of gingival fibroblasts and on the collagen and protein synthesis of gingival fibroblasts. Gingival fibroblast from giniva of first bicuspids from patient for orthodontic treatment were used and cultured. For the measurement of inhibitory ability of collagenolytic activity, crude enzyme was extracted and used on the basis of modified Ono's method. On the inhibition of collagenolytic enzyme from herbal extracts, collagenokit CLN-100 were used. The cellular activity of gingival fibroblast, were studied using MTT solution and measured optical density on 570mm by ELISA reader. To measure the effects on the ability of whole protein and collagen synthesis, cell membrane was destructed with ultrasonic grinder after culturing, centrifuged and counted by liquid scintilation counter. The inhibitory effects on producing of IL-1β by monocyte, after promotion of producing IL-1β by LPS, were compared with the mixture of herbal extracts and other drugs using thymocyte stimulation assay. About inhibitory·effects of PGE_2 by gingival fibroblasts, herbal extract was compared with the addition of the other control groups using enzyme immunoassay. On the inhibition of collagenolytic activity by P. gingivalis, benzene extracts showed the most efficient inhibitory effects among the l9㎍/㎖ of the compared extracts and 40.5% by Tetracycline. On the cellular activity promoting effects, compared extracts showed a bit of more effects than PDGF of l00㎍/㎖ concentration and lGF of 20㎍/㎖ concentration. All of the PDGF, 1GF, Zizyphi Fructus extract should increase in collagen synthesis, but especially 70% ethylalcohol extracts of Zizyphi Fructus showed comparably high effects among the compared extracts. Effects on whole protein synthesis were slightly increased on every extract but especially 70% ethylalcohol extract showed significantly effective than any other estract. On the inhibitory effects of Zizyphi Fructus IL-1β production by monocyte, compared extracts showed 70% of highly inhibitory effect than that of 60% inhibition effects on controlled group and each extracts showed no significant difference. In PGE_2 production inhibitroy effect of Zizyphi Fructus gingival fibroblasts, Herbal extracts showed 70% of inhibition comparing with tat of 90.2% of controlled group, but each extracts showed similar effects excluding the H_2O extracts. These results suggested that Zizyphi Fructus might be useful medicine for inhibition of inflammatory mediator including IL-1β and PGE_2·

貧血症과 赤血球過多症에 있어서 血淸 Erythropoietin 活性度에 關한 硏究

尹起英,李夏白,朴鍾茂 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.2

Erythropoietin (Ep) is a glycoprotein hormone of mostly renal origin that stimulates red cell production in response to tissue hypoxia. Serum Ep activities provide important clinical clues in making the differential diagnosis of some types of anemia and polycythemia. The study was performed in order to evaluate the diagnostic value of serum Ep activities in 29 normal subjects, 43 anemic patients and 15 secondary polycythemia patients and to find out the relationship between hemoglobin concentration and serum Ep activities in these patients using the Enzyme Immunoassay with Second International Preparation for Ep. The results obtained were as follows: 1. Ep activities in sera from 29 normal subjects among children ranged from 11 to 72 mU/ml, with a mean of 37.10±16.54 mU/ml. There was no significant difference between the serum Ep activities in male and female subjects (p>0.05). 2. Ep activities in sera from 22 patients with iron deficiency anemia ranged from 48 to 400 mU/ml, with a mean of 153.5 mU/ml which was about 4 times higher than normal value. Ep activities in sera from 18 anemic patients with acute infection ranged from 35 to 290 mU/ml, with a mean of 134.05 mU/ml which was about 3.5 times higher than normal value. Ep activities in sera from 3 patients with aplastic anemic ranged from 620 to 840 mU/ml, with a mean of 720 mU/ml which was about 20 times higher than normal value. 3. Ep activities in sera from 15 patients with secondary polycythemia ranged from 12 to 140 mU/ml. In 10 of 15 patients, the mean Ep activities was 87.50 ± 25.60 mU/ml, which was about 2 times higher than normal value. In 5 of 15 patients, Ep activities were within normal range as a mean of 35.80 ± 17.23 mU/ml. In summary, a significant negative correlation was found between the hemoglobin concentration and the logarithm of the serum Ep activities in 43 patients with anemia (r=0.73, Y=3.6766-0.1802x) and Ep activities in patients with aplastic anemia was higher than those in patients with iron deficiency anemia at comparable hemoglobin concentration. From the above results, it was concluded that serum Ep activities in anemic patients are not only inversely related to the hemoglobin concentration but also to the activity of the erythroid bone marrow. While no significant correlation was found between hemoglobin concentration, hematocrit percentages and Ep activities in 15 patients with secondary polycythemia (r=0.18, 0.39 respectively).

Transforming Growth Factor (TGF)-β1 Releasing Tricalcium Phosphate/Chitosan Microgranules as Bone Substitutes

Lee, Jue-Yeon,Seol, Yang-Jo,Kim, Kyoung-Hwa,Lee, Yong-Moo,Park, Yoon-Jeong,Rhyu, In-Chul,Chung, Chong-Pyoung,Lee, Seung-Jin 이화여자대학교 약학연구소 2004 藥學硏究論文集 Vol.- No.14

Purpose. Tricalcium phosphate (TCP)/chitosan composite microgranules were developed as bone substitutes and tissue engineering scaffolds with the aim of obtaining a high bone forming efficacy. The microgranules have the ability to fill various types of defect sites with closer packing. In addition. the transforming growth factor-beta I(TGF-β1) was added to the microgranules in order to improve bonehealing efficacy. Methods. TCP/chitosan microgranules were fabricated by dropping a TCP suspended chitosan solution into a NaOH/ethanol solution. TGF-β1 was incorporated into the TCP/chitosan microgranules by soaking the microgranules into the TGF-β1 solution. Scanning electron microscopy(SEM) observations as well as experiments examining the release of TGF-β1 from chitosan and TCP/chitosan microgranules were performed. SEM was used to examine the cell morphologies on the microgranules, and the extent of cell proliferation was evaluated using a dimethyl-thiazol tetrazolium bromide (MTT) assay. The differentiated cell function was assessed by measuring the alkaline phosphatase activity as well as performing an osteocalcin assay. Results. The size of the prepared microgranules was 350-500㎛ and TCP powders were observed on the surface of the microgranules. TGF-β1 was released from the TCP/chitosan microgranules at a therapeutic concentration for 4 weeks. The proliferation of osteoblasts on the TGF-β1 loaded microgranules was the highest among the microgranules. SEM indicated that the seeded osteoblastice cells were firmly attached to the microgranules and proliferated in a multilayer fashion. The ALPase activity and osteocalcin content of all the samples increased during the culture period Conclusions. These results suggest that the TCP/chitosan microgranules are potential bone substitutes with a drug releasing capacity and a osteoblastic cells culture scaffold.

Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials

Lee, Jue-Yeon,Nam, Sung-Heon,Im, Su-Yeon,Park, Yoon-Jeong,Lee, Yong-Moo,Seol, Yang-Jo,Chung, Chong-Pyoung,Lee, Seung-Jin 梨花女子大學校 藥學硏究所 2002 藥學硏究論文集 Vol.- No.11

For the pulpose of obtaining high bone forming efficacy. developlment of chitosan was attempted as a tool useful as ascaffolding device. Porous chitosan matrices, chitosan-poiy(L-lactide) (PLLA) composite matrtices and chitosan coated onPLLA matrices were dealt with in this research. Porous chitosan matrix was fabricated by freeze-drying and cross-linkingaqueous chitosan solution. Porous chitosan matrix combined with ceramics and constituents of extracellular matrices wereprepared and examined for their bone regenerative potential. Composite porous matrix of chitosall-PLLA was prepared bymixing polyiactide with chitosan and freeze-drying. Al1 chitosan based devices demonstrated improved bone formingcapacity by increasing mechanical stability and biocompatibility. Release of platelet-derived growth factor-BB (PDGf-BH )from these matrices exerted significant osteoinductive effect in addition to the higf esteocdElducting capacity of the porouschitosan matrices. The hydrophobic surface of PLLA matrices was modified by chitosan to enhance cell affinity andwettability, The chitosan coafed PLLA matrix induced increased osteoblast attachment as compared with intact PLLAsurface. Overall results in this study demonstrated the usefulness of chitosan as drug releasing scaffolds and as modificationtools for currently used biomaterials to enhance tissue regeneration efficacy. These results may expand the feasibilitr ofcombinatfve strategy of controlled locai drug delivery concept and tissue engineered bone formation in reconstructivetherapy in the field of periodontics, orthopedics and plastic surgery.