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The Effect of Azithromycin on the Cyclosporin-Ainduced Gingival Fibroblast Overgrowth
노현수,정원윤,최성호,조규성,박광균,Noh, Hyuen-Soo,Chung, Won-Yoon,Cho, Seong-Ho,Cho, Kyoo-Sung,Park, Kwang-Kyun The Korean Academy of Periodontoloy 2003 Journal of Periodontal & Implant Science Vol.33 No.4
Cyclosporin-A(CsA)는 장기와 조직 이식에 따른 거부반응을 조절하기 위해 사용되는 면역억제제로, 이식의학의 발달과 더불어 사용량이 증가하고 있다. CsA의 부작용중의 하나인 치은과증식은 30-50%의 빈도로 발발하고 있다. 최근 macrolide 계열의 항생제인 azithromycin을 이용하여 이런 부작용을 억제시킨다는 임상 보고가 있어서, 이를 실험적으로 확인하고자 하였다. 이를 위해 CsA를 투여한 적이 없는 환자에서 정상 치은조직을 채취, 치은섬유아세포를 배양하였다. 우선 CsA에 대한 치은섬유아세포의 반응을 보기 위해 다양한 농도($10^{-8}-10^{-10}$g/ml)로 처치하여, 세포 증식량과 교원질 합성량을 MTT assay와 Sirol Collagen Assay를 이용하여 측정하였다. 이에 반응을 보인 조건과 세포를 대상으로 다양한 농도($10^{-8}-10^{-10}$g/ml)의 azithromycin을 CsA와 동시 처치하여 아래와 같은 결과를 얻었다. 1. CsA는 일부 치은섬유모세포의 증식을 증가시켰다. 그러나 Collagen 합성능에는 변화를 주지 않았다. 2. Azithromycin은 정상 치은섬유아세포의 증식능에 영향을 미치지 않았다. 3. Azithromycin은 CsA 에 반응을 보인 세포의 증식을 감소시켰으며, 이는 정상 수준과 유사하였다. 이상의 결과에서 azithromycin이 CsA에 의한 치은과증식 치료에 유익하다고 사료된다.
김현정,정원윤,황재관,박광균 대한암예방학회 2013 Journal of cancer prevention Vol.18 No.1
Xanthorrhizol, isolated from Curcuma xanthorrhiza, has been shown to induce cell cycle arrest and apoptotic cell death by mainly mitochondrial-dependent signaling pathway in several human cancer cells. We investigated whether xanthorrhizol could apoptosis in HL-60 human promyelocytic leukemia cells. Xanthorrhizol treatment inhibited cell viability and induced apoptotic cell death. The levels of procaspase-3, -8 and -9 were reduced and the cleavage of full length PARP was clearly observed in xanthorrhizol-treated cells. Xanthorrhizol treatment did not affect Bcl-2 protein level but increased Bax protein level, resulting in the diminished Bcl-2/Bax ratio. The levels of cleaved Bid, Fas death receptor and p53, not FasL, were also increased by xanthorrhizol treatment. In conclusion, molecular cross talks between the intrinsic and extrinsic apoptotic pathway via Bid may play an important role for induction of apoptosis in xanthorrhizol-treated HL-60 cells. Therefore, xanthorrhizol has the chemorpeventive and anti-cancer potential against human promyelocytic leukemia cells.
이창기,이상국,정원윤,박광균,황재관 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.2
The fruit of Prunus persicaL. (peach) is one of the common fruits. Its seed is well known as a traditionalmedicine (Persicae Semen) in Japan, China, and other Asian countries. However, the biological activities of P. persicafruitexcept its seed are poorly understood. This study was aimed at evaluating the protective effect of the pericarp extract of P.persica(PPE) against cisplatin-induced acute toxicity in mice. PPE (500 mg/kg, p.o.) showed a significant protection againstthe acute nephrotoxicity and hepatotoxicity induced by a single administration of cisplatin (45 mg/kg, i.p.) over a 16-hour pe-riod in mice. Its protective effect was evaluated by serum and tissue biochemical parameters. The pretreatment with PPE for7 days prevented the cisplatin-induced decrease in the kidney and liver weights as a percentage of the total body weight. PPEsignificantly inhibited both the cisplatin-induced elevation in serum blood urea nitrogen and creatinine levels caused by kid-ney damage and the cisplatin-induced increase in serum alanine aminotransferase and aspartate aminotransferase levels by theliver damage. In addition, the administration of PPE caused recovery of the cisplatin-mediated changes in levels of serum ni-tric oxide and tissue lipid peroxidation, and reduced glutathione content returned to control levels. These results suggest thatPPE protects against cisplatin-induced nephrotoxicity and hepatotoxicity by reducing cisplatin-induced oxidative stress in mice.
Sunghwa Han,정원윤,이상국,Jaekyung Kim,황재관,박광균 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.6
In this study, antioxidant and anti-photoaging activities of 2-methoxy-5-(2-methyl propyl) pyrazine (MMPP) isolated from peach (Prunus persica (L.) Batsch)were investigated in terms of matrix metalloproteinase (MMP)-1 and type-1 procollagen expression in ultraviolet (UV)-irradiated human skin fibroblasts. MMPP was obtained from the butanol fraction of a 95% ethanol extract of peach. The antioxidant and anti-photoaging activity of MMPP were assessed by 1,1-diphenyl-2-picrylhydrazy (DPPH), Western blot, and reverse trans-cription-polymerase chain reaction (RT-PCR) analyses. MMPP displayed radical scavenging activity, suppressed MMP-1 expression,and increased type-1 procollagen expression. These results support the use of MMPP from peach as an antiphotoaging compound.
Polymorphisms of CYP1A1 and GSTM1 Genes and Susceptibility to Oral Cancer
차인호,박종윤,정원윤,최민아,김형준,박광균 연세대학교의과대학 2007 Yonsei medical journal Vol.48 No.2
Purpose: Oral cancer is the fifth most common form of cancer in the world and comprises 6.5% of all cancer deaths. Since one of the major risk factors for oral cancer is tobacco use, we hypothesized that polymorphic genes coding for tobacco carcinogen-metabolizing enzymes may play a role in oral cancer susceptibility. Materials and Methods: To investigate the association between polymorphisms of the CYP1A1 and GSTM1 genes and risks for oral squamous cell carcinoma (OSCC) in the Korean population, the prevalence of the CYP1A1 Mspl and GSTM1 null polymorphisms were examined in 72 patients with histologically confirmed primary OSCC, as well as in 221 healthy control subjects. Results: A significant risk increase for oral cancer was observed among subjects with the homozygous CYP1A1 (m2/m2) genotype (OR=3.8, 95% CI=1.9-7.7), but not the GSTM1 null genotype (OR=0.7, 95% CI=0.4-1.3). Risk for oral cancer was significantly increased in subjects with the homozygous CYP1A1 (m2/m2) genotype, regardless of smoking history (smokers; OR=4.4; 95% CI=1.2-16.3; non- smokers OR=4.9; 95% CI=1.9-12.5). Using the potentially most protective genotype GSTM1 (+)/CYP1A1 [(m1/m1)+ (m1/m2)] as the reference group, an increased risk for oral cancer was observed among subjects with the GSTM1 (+)/ CYP1A1 (m2/m2) (OR= 2.0, 95% CI=0.8-5.2), and GSTM1 (-)/ CYP1A1 (m2/m2) (OR=4.9, 95% CI=1.5-15.5) genotypes (p<0.009, (χ2 trend test). Conclusion: Our results suggest that individuals with a genotype of CYP1A1 (m2/m2) and GSTM1 (-) are highly susceptible for OSCC and that the CYP1A1 (m2/m2) genotype is closely associated with increased risk of OSCC in Koreans.
김현정,박광균,정원윤,이선경,김기림 대한암예방학회 2017 Journal of cancer prevention Vol.22 No.1
Background: Nicotine is a major toxic component of tobacco smoke and has been recognized as a risk factor to induce oxidative tissue damage, which is a precursor to cardiovascular diseases, lung-related diseases, and cancers. Peaches (Prunus persica) have been used for the treatment of degenerative disorders, such as hypermenorrhea, dysmenorrhea, and infertility in Asian countries. In this study, we investigated the effects of white-fleshed peach on the excretion of nicotine metabolites and 1-hydroxypyrene in smokers and chronic nicotine-induced tissue damages in mice. Methods: The concentrations of cotinine and 1-hydroxypyrene were measured in urine of smokers before or after intake of white-fleshed peaches. In addition, ICR mice were injected with nicotine (5 mg/kg body weight) and then orally administered with white-fleshed peach extracts (WFPE) (250 or 500 mg/kg body weight) for 36 days. The oxidative stress parameters and the activities of antioxidant enzymes were measured in liver and kidney tissues. Also, histological changes and nitrotyrosine expression were assessed. Results: Intake of white-fleshed peaches increased the urinary concentration of nicotine metabolites and 1-hydroxypyrene in 91.67% and 83.33% of smokers, respectively. WFPE decreased the malondialdehyde levels and recovered the activities of antioxidant enzymes in nicotine-injected mice. In addition, WFPE inhibited nitrotyrosine expression and inflammatory responses in the liver, kidney, and lung tissues of nicotine-treated mice. Conclusions: White-fleshed peaches may increase the metabolism of toxic components in tobacco smoke in smokers and protect normal tissues against nicotine toxicity in mice. Therefore, supplementation of white-fleshed peaches might be beneficial to smokers.