저근백피(Ailanthus altissima) 물 추출물에 의한 급성림프성백혈병 Jurkat T Lymphocytes의 세포고사 유도

황상구,이형철,김춘관,천현자,정승일,전병훈,Hwang, Sang-Gu,Lee, Hyung-Chul,Kim, Chun-Kwan,Chun, Hyun-Ja,Jeung, Seung-Il,Jeon, Byung-Hun 한국생약학회 2001 생약학회지 Vol.32 No.4

Ailanthus altissima belonging to the family Simaroubaceae has been used to settle an upset stomach, to combat a fever, and as an insecticide. Apoptosis is an active process, which is a critical feature of the regulated development of multicellular organisms. We investigated whether the extract of A. altissima induced apoptotic cell death in Jurkat T-acute lymphoblastic leukemia (ALL) cells. Upon treatments with the extract, the dose-dependent inhibitions of cell viability were observed. It also caused apoptosis as measured by cell morphology and DNA fragmentation. The capability of the extract to induce apoptosis was associated with proteolytic cleavage of specific target protein such as poly(ADP-ribose)polymerase (PARP) protein, suggesting the possible involvement of the activations of caspases. Further study showed that Bcl-2 protein levels were not changed in all treated groups compared to control group. These results suggest that A. altissima induces Bcl- 2-independent apoptosis in Jurkat T cells.

Quercetin이 B16/F10멜라닌세포의 증식 및 멜라닌화에 미치는 영향

천현자(Hyun Ja Chun),황상구(Sang Gu Hwang),김춘관(Chun Kwan Kim),전병훈(Byung Hun Jeon),백승화(Seung Hwa Baek),우원홍(Won Hong Woo) 대한약학회 2002 약학회지 Vol.46 No.1

Quercetin is one of the bioflavonoid compounds and has multiple biological effects such as antioxidant and effective anti-inflammatory agent. Melanin has an important role in protecting human skin from the damaging effects of ultra-violet (UV) radiation. We studied the effect of quercetin on proliferation of B16/F10 melanoma cells. After 48h treatment of cells with quercetin, the cells exhibited a dose- dependent inhibition in their proliferation without apoptosis. Therefore, the decrease in cell numbers may be due to cell growth arrest, not due to cell death by cytotoxiciy. We also investigated the effect of quercetin on melanogenesis of this cells. B16/F10 melanoma cells were grown for 48h in the presence of 0.01∼50μg/ml Quercetin and the total melanin contents were measured. Quercetin stimulated melanization of the cells in low concentrations (0.01-20μg/ml), whereas it inhibited melanization in high concentrations (30-50 μg/ml). It was observed that quercetin differently regulates melanogenesis of B16/F10 melanoma cells dependent on its concentrations.

Jurkat T 임파구의 세포주기 기전에 미치는 저근백피 ( Ailanthus altissima)의 효과

황상구(Sang Gu Hwang),이형철(Hyung Chul Lee),김춘관(Chun Kwan Kim),김대근(Dae Geun Kim),이기옥(Gi Oug Lee),윤용갑(Young Gab Yun),전병훈(Byung Hun Jeon) 대한약학회 2002 약학회지 Vol.46 No.1

Ailanthus altissima has been used to settle an upset stomach, to alleviate a fever and as an insecticide. We reported that the water extract of A. altissima induced apoptotic cell death in Jurkat T.acute Iymphoblastic leukemia cells. Herel we showed the dose-dependent inhibitions of cell viability by the extract, as measured by cell morphology The cell cycle contro1 genes are considered to play important roles in tumorigenesis. The purpose of the present study is also to investigale the effect ot A. altissima on cell cycle progression and its molecular mechanism in the cells. The level of p21-proleln was Increased after treatment ot the extract, whereas both Bcl-2 and Bax protein levels were not changed. These resu1ts suggest that A. altissima Induces apoptotic cel1 death via p2l- dependent signaling pathway in furkat cells which oelete wlld type p53 checkpoint related gene products tested (cyclin D3, cyclin dependent kinase 4, retinoblastoma E2F1) were decreased In their. Protein levels in a dose-dependent manner after treatment of the extract. Taken together, these resu1ts inulcate that the increase of apoptotlc cell death by A. altissima may be due to the inhibition of cell cycle in Jurkat cells.

백혈병세포주 Jurkat T 세포에서 합환피 Albizzia julibrissin 물 추출물의 아포토시으 유도 효과

황상구(Sang Gu Hwang),이형철(Hyung Chul Lee),김춘관(Chun Kwan Kim),김용익(Yong Ik Kim),주성민(Sung Min Ju),김원신(Won Sin Kim),전병훈(Byung Hun Jeon) 대한약학회 2001 약학회지 Vol.45 No.6

Apoptosis is a morphologically and biochemically distinct form of cell death that occurs in many different cell types in a wide variety of organisms.Albizzia julibrissin belonging the family Leguminosae has been used for the treatment of contusion, sore throat, amnesia, and insomnia in oriental traditional medicine. This study investigates whether the water extract of A. julibrissin induce apoptotic cell death in Jurkat T-acute Iymphoblastic leukemia (ALL) cells. Jurkat cells were increased inhibitions of cell viability in a concentration-dependent manner by A. julibrissin. This herbal medicine also caused apoptosis as measured by cell morphology and DNA fragmentation. The capability of A. julibrissin to induce apoptosis was associated with proteolytic cleavage of specific target protein such as poly (ADP-ribose) polymerase (PARP) protein suggesting the possible involvement of caspases. Our result showed that Bcl-2 and Bax protein levels were not changed in all A. julibrissin-treated groups compared to control group. These results suggest that A. julibrissin-mediated apoptosis is independent with Bcl-2 related signaling pathway in this cells.

항암화학요법 중 호중구감소증이 발생한 저위험군 발열 환자들을 대상으로 한 경구 항균제 요법의 임상적 유용성 및 안정성에 대한 연구

김연숙,이혁,기현균,김춘관,김신우,김성민,백경란,김원석,윤성수,이홍기,강원기,박찬형,박근칠,송재훈 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.1

목적 : 항암화학요법 중 호중구감소증을 동반한 발열이 발생하는 암환자들을 치료하기 위한 다양한 항균제와 여러 가지 방법들이 시도되고 있는 가운데, 합병증과 사망률의 발생가능성이 적은 저위험군 환자들을 대상으로 초기 72시간동안 정주 항균제를 투여한 이후 경구 항균제로 전환하는 요법의 유용성과 안정성을 평가해보고자 본 연구를 시행하였다. 방법 : 1998년 2월부터 1999년 9월까지 본원에서 항암화학요법 중 호중구감소증과 발열이 발생한 환자들 가운데 기저 암질환이 고형암이거나 림프종이고, 입원당시 패혈증의 증후가 없으며 입원 72시간이내에 해열되고 백혈구수치가 증가 추세인 환자들을 대상으로 하여 72시간 동안 정주 항균제를 투여한 이후 경우 ciprofloxacin 750㎎을 하루 2회씩 투여하여 총 4일간 투여하였다. 모든 환자들은 열이 떨어지고 호중구감소증이 회복될 때까지 입원하도록 하였다. 결과 : 총 38명 환자의 40예가 등록이 되었고, 환자들의 기저암 질환은 고형함이 72.5%, 림프종이 27.5%였다. 입원당시 평균 호중구치수는 156/㎕였고, 호중구수치가 100/㎕미만인 경우는 65%였으며, 호중구감소증이 지속된 기간의 평균은 2.4일이었다. 40예 중 39예가 항균제의 변형이나 추가 없이 호중구감소증과 발열로부터 회복이 되어 97.5%의 성공율(95% 신뢰구간: 86.8-99.9%)을 보였다. 부작용으로 피부발진이 있었던 경우가 한 예 있었는데, 증상이 경하여 경구 항균제를 지속할 수 있었다. 심와부의 동통으로 복용을 지속할 수 없어서 대상에서 제외된 예가 또 한 예 있었다. 결론 : 항암요법 중 호중구감소증과 발열을 동반한 환자들 가운데 저위험군 환자들에서 항균제 72시간정주 이후 경구 항균제로의 전환요법은 효과적이고도 안전한 치료방법이라고 할 수 있다. Background : Oral antibiotic therapy following empirical intravenous antibiotics may be effective and safe for febrile neutropenic patients with lowrisk for complications. Methods : We conducted a prospective clinical trial of oral antibiotic therapy in the patients with neutropenia and fever during chemotherapy for cancer. Underlying malignancies were solid tumor or lymphoma with short duration of neurtropenia and the patients had no evidence of clinically or microbiologically documented infections. Oral ciprofloxacin was given to the patients who lacked signs of sepsis on admission, had a rising tendency of neutrophil count (ANC >100 /㎕ ) at 72 hours, and were afebrile at 72 hours. All patients were hospitalized until neutropenia and fever resolved. Results : A total of 40 episodes of 38 patients were enrolled from February 1998 to September 1999. The mean neutrophil counts on admission were 156/㎕ and the mean duration of neutropenia was 2.4 days. The episodes which had neutrophil count below 100 /㎕ were 26 (65%). Treatment was successful in 39 of 40 episodes (97.5% : 95 % confidence interval, 86.8% to 99.9%). Adverse reactions of oral ciprofloxacin were skin rash and epigastric soreness in two cases, respectively. There were no deaths during the study. Conclusions : For low-risk febrile patients with neutropenia during cancer chemotherapy, switch therapy to oral ciprofloxacin at 72 hours following intravenous broad-spectrum antibiotics is effective and safe,

간이식 환자에게서 발생한 이식 후 림프세포 증식성 질환 2예

김성민,김성주,이석구,김춘관,조재원,송재훈,박정호,박상종,이혁,고영혜,백경란 대한내과학회 1999 대한내과학회지 Vol.56 No.3

The posttransplantation lymphoproliferative disorders (PTLD) are not rare complications of solid organ transplants. The incidence varies with the type of transplantation and the nature and intensity of the immunosuppressive regimens. PTLDs are unique in that they have a predilection for extranodal sites, a strong and probably causal association with Ebstein-Barr virus infection, and a poor response to the cytolytic chemotherapeutic or irradiation regimens used for treatment of malignant lymphoma. The outcomes of treatment have been disappointing, with mortality from PTLD or related complications of over 50% of patients. We experienced two cases of PTLDs in liver transplant recipients presenting with liver mass and intraabdominal lymphadenopathy. PTLDs were diagnosed by autopsy and a liver biopsy. In the case diagnosed by a liver biopsy, EBV was detected by in situ hybridization. Despite reduction of immunosuppression and conservative management, both patients died.

유백피 메탄올 추출물에 의한 Melanin 합성 조절

정승일,김춘관,전병훈,백승화,우원홍,천현자 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.5

Melanogenesis is a physiological process resulted in the synthesis of melanin pigments, which have a role in protecting skin from the damaging effect of ultra-violet(UV) radiation. The main aim of the present study was to examine the effect of Ulmus davidiana var. japonica on melanogenesis. Cells were cultured in the presence of methanol extracts from Ulmus davidiana var. japonica for 48 h, and there were estimated total melanin content as a final product and activity of tyrosinase, a key enzyme, in melanogenesis. Methanol extracts of the bark Ulmus davidiana var. japonica increased tyrosinase activity and melanin contents in a dose-dependent manner. Especially, It was observed that only 100 μg/ml methanol extract stimulates melanin secretion in B16/F10 melanoma cells by 140% at 48 h treatment and activity of tyrosinase increased by 250 % in the presence of same concentration.

p53-결핍 Jurkat T 임파구의 세포사멸에 대한 鷄血藤 물 추출물의 세포주기 억제효과

황상구,이형철,김춘관,김대근,송호준,박영준,김원신,전병훈 대한동의생리학회,대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.6

Spatholobus suberectus belonging to the family Leguminosae has been used in Oriental medicine for promoting blood circulation, removing blood stasis, tonifying the blood, relaxing tendons, stopping internal bleeding and eliminating dampness. This study investigates whether the water extracts of S. suberectus induce apoptotic cell death in Jurkat T-acute lymphoblastic leukemia (ALL) cells. Cell viability of Jurkat cells was inhibited in a concentration-dependent manner by S. suberectus, as measured by cell morphology. The capability of S. suberectus to induce apoptosis was associated with proteolytic cleavage of specific target protein such as poly (ADP-ribose) polymerase protein suggesting the possible involvement of caspases. The purpose of this study is also to investigate the effect of S. suberectus on cell cycle progression. G1 checkpoint related gene products (cyclin D1, cyclin dependent kinase 4. retinoblastoma, E2F1) were decreased in their protein levels in a dose-dependent manners after treatment of the extract. These results indicate that the increase of apoptotic cell death by S. suberectus may be due to the inhibition of cell cycle progression in wild type p53-lacking Jurkat cells.