淫羊藿이 Tyrosinase 酵素活性및 Melanin 生成에 미치는 影響

千賢子,金容隨,金台英,金在柱,安成薰,田炳薰,禹元洪 한국전통의학연구소 2000 한국전통의학지 Vol.10 No.2

With a view to investigating how Epimedium koreanum Nakai affects formation of melanin content in skin, we have measured change in the number of cell, melanin content, and tyrosinase activity by treating its aqueous extracts at various concentrations with B16 melanoma cells, the result of which is as follows: Firstly, cell viability has been shown as over 95% when treating with the aqueous extract of Epimedium koreanum Nakai up to 200 ug/mL, from which the effect of cell toxicology has not nearly appeared. Secondly, the number of cell has been slightly decreased than that of the control group when treating with the aqueous extract of Epimedium koreanum Nakai, which has not shown a statistical noteworthiness. Thirdly, the aqueous extract of Epimedium koreanum Nakai has increased in tyrosinase activity. Fourthly, the aqueous extract of Epimedium koreanum Nakai has promoted the formation of melanin content, its final product. According to the result, it is confirmed that Epimedium koreanum Nakai has the influence on promoting melanization of B16 melanoma cell, and it is considered that Epimedium koreanum Nakai can be applicable for curing vitiligo caused by decrease or loss of pigment through a systematic study.

Quercetin의 Melan-a 멜라닌세포의 멜라닌합성에 미치는 영향

천현자,최원형,백승화,우원홍 한국생약학회 2002 생약학회지 Vol.33 No.3

근육피로가 혈액과 근조직의 대사활성에 미치는 영향 및 뜸의 효과 Ⅱ

千賢子,金日光,張敬善 圓光大學校 基礎自然科學硏究所 1993 基礎科學硏究誌 Vol.12 No.1

After moxibustion to Jog Sam Ri(S 36), measurement of the changes in the concentrations of creatinine and lactate, and in LDH actibity, i.e. fatigue material, as a moxibustion therapy of muscle fatigue, we have concluded as follows; An increase both in blood serum and muscular tissue of the experiment group than the control group, which has shown a same trend in proportion to swimming time. The concentration of creatinine both in blood serum and muscular tissue within the experiment group with moxibustion has shown a decrease, which has shown a same figure in case of lactate. LDH activity in the experiment group has shown an entire decrease in comparison with the control group, and the ratio of decrease has been similar with that of lactate. The whole values of concentrations of creatinine and lactate, and LDH activity have shown a gradual decrease according to the amount of moxibustion.

근육피로가 혈액과 근조직의 대사활성에 미치는 영향 및 뜸의 효과 Ⅰ

千賢子,金日光,韓宗賢,林善英,張敬善 圓光大學校 基礎自然科學硏究所 1993 基礎科學硏究誌 Vol.12 No.1

As a result of measuring a changes in the concentrations of creatinine and lactate, and LDH activity, produced in rat blood serum and muscular tissue in case of muscular exercise by swimming, with a view to examining the influence of fatigue upon muscle metabolism, we have concluded that the values have gradually increased in blood serum and muscular tissue of the experiment group rather than the control group in proportion to swimming time. On the one hand, the concentration of creatinine both in blood serum and muscular tissue within the experiment group with moxibustion to Can Su(B 18) has showed a prominent decrease in comparison with the control group(P < 0.01). The concentration of lactate in muscular tissue has decreased 2.2 times(75%) more than in blood serum (34%). From the above result, moxibustion, having an anti-fatigue effect, helps the rising of exercise ability, which, for me, results from the decrease in the concentrations of creatinine and lactate, i.e. material of fatigue, from the prohibition of LDH activity, and from the effect on anti-fatigue in muscle.

포스터발표 / P-49 : 사과와 토양에서 Iminoctadine Triacetate 의 잔류와 반감기

천현자,정승일,한성수,김일광 한국분석과학회 1999 학술대회논문집 Vol.- No.-

蘇木의 에틸아세테이트 추출물이 B16/F10 흑색종세포의 멜라닌 합성에 미치는 효과

천현자,김용수,남항우,윤성찬,우원홍 대한동의생리학회,대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.6

Caesalpinia sappan L. has been commonly used as analgesic, a cure for contusion and sprain as well as a remedy for thrombosis in the oriental medicine. The main constituent of Caesalpinia sappan L. is brazilin having a flavonoid structure. Melanin is a main pigment found in skin, hair and eyes, and melanin synthesis is controlled by a cascade of enzymatic reactions regulated at the level of tyrosinase. The aim of this study was to examine the effect of Caesalpinia sappan L. on proliferation and melanogenesis of B16/F10 melanoma cells. After 48h treatment of cells with various concentrations of EtOAc extract, the cells exhibited a dose-dependent inhibition in their proliferation without apotosis. Therefore, the growth retardation may be due to the cell arrest, not due to cytotoxicity. And there were estimated total melanin contents as a final product and activity of tyrosinase, a key enzyme, in melanogenesis. EtOAc extract increased the melanin contents and tyrosinase activity in low concentration (0.01-10㎍/ml), whereas it decreased those in high concentration. In conclusion, it was observed that EtOAc extract of Caesalpinia sappan L. differently regulates melanization of cells dependent of its concentrations.

소목의 부탄올 추출물에 의한 Melan-a 세포의 멜라닌생성 억제효과

천현자,황상구,이진선,백승화,전병훈,우원홍 한국생약학회 2002 생약학회지 Vol.33 No.2

산수유 헥산 추출물의 항균효과 및 세포독성

천현자,최원형,이정호,양현옥,백승화 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.2

Cornis fructus was extracted by successive extraction and then fractionated with hexane extract to get active is study was performed to determine the cytotoxic effect of hexane extract from Comis fructus on NIH 3T3 fibroblasts and cancer cell lines using MTT assay. Hexane extract showed cytotoxic effect against A549, B16 melanoma and MDA-MB-231. Futher fractionation with hexane extract was performed to obtain effective fraction, fraction 3 showed the cytotoxic effect against A549 and MDA-MB-231. In antimicrobial test of each fraction of hexane extract, fraction 5 showed antimicrobial activities against P. putida and P. aeruginosa.

음양곽과 $\alpha$ -MSH에 의한 B16 Melanoma 세포의 멜라닌화와 Retinoic Acid의 억제 효과

천현자,김일광,우원홍,Chun, Hyun Ja,Kim, Il kwang,U, Won Hong 대한화학회 2000 대한화학회지 Vol.44 No.6

Melanization of B16 melanoma cells was comparatively studied by the aqueous extract of Epimedium koreanum Nakai(EK) and $\alpha$-MSH. In addition, inhibitory effects of RA was investigated. B16 melanoma cells(about 1${\times}10_5$) have been shown an increase in tyrosinase activity and melanin contents in proportion to concentration of $\alpha$-MSH when treated with $\alpha$-MSH and incubated for 72 hrs. They indicated a 350% increase in tyrosinase activity and a 290% increase in melanin contents at 8 ng/mL. In case of EK, they have been shown a 200% increase in tyrosinase activity and a 180% increase in melanin contents at 100 ${\mu}g$/mL. In addition of RA to the above condition, they have been shown an inhibition from 350% to 210% in tyrosinase activity and from 290% to 250% in melanin contents in $\alpha$-MSH, and inhibition from 200% to 100% in tyrosinase activity and from 180% to 120% in melanin contents in EK. From the above results, it is suggested that EK promotes melanization of B16 melanoma cells through cAMP pathway, whereas RA inhibits it.

마이셀용액에서 크롬(Ⅴ1)의 전기화학적 환원에 관한 연구

김일광,성낙도,천현자 圓光大學校 1984 論文集 Vol.18 No.2

The electrochemical reaction of chromium (Ⅵ at a static mercury electrode in micelle aqueous solution has been examined by DC polarography, DP polarography and cyclic voltammetry. The polarographic and cyclic voltammetric data shown to be chemical reactively irreversible process of chromium (Ⅵ) electrode reaction. the height of reduction waves and halfwave potentials were shifted to the more negative patential in the NaLS, CTABr solution. When chromium (Ⅵ) ion was reduced in basic solution, the following possible mechanism was portulated. ?? In acidic solution, the reduction of chromium (Ⅵ), dimer proceeds by a six-electron transfer to form chromium (Ⅲ). Therefore the following reduction was suggested. ??