갈근(葛根)의 항산화작용에 의한 위점막 보호효과와 내인성 Sulfhydryl 화합물의 영향

최호정,신흥묵,Choi, Ho-Jeong,Shin, Heung-Mook 대한동의생리학회 1999 동의생리학회지 Vol.14 No.2

Ethanol induces compoundhemorrhagic gastric lesions and causes a dose-dependent decrease in the concentration of endogenous nonprotein sulfhydryls in rat gastric mucosa. Sulfhydryl-containing drugs protect rats from ethanol - induced gastric lesions. Based on this findings, we investigated the involvement of sulfhydryl compounds in the antioxidant effects of Puerariae radix, a traditional herbal medicine, against ethanol - induced gastric lesions in the absence and presence of iodoacetamide(IDA. sulfhydryl blocking agent) in rats. respectively. Because of the known role of sulfhydryls in gastric cytoprotection, its role in gastric antioxidation was of intrest. In vitro, Puerariae radix extract(PRE) reduced linoleic acid autooxidation and exert DPPH radical scavenging effect. In vivo. PRE increased antioxidants(SOD, catalase. GSH) and reduced lipid peroxide level in ethanol-induced gastric mucosal lesions. But treatment with PRE plus IDA significantly inhibit the antioxidant effects such as SOD and GSH but did not affect catalase levels. These results suggest that Puerariae radix may play roles in the gastric cytoprotection through antioxidant effects and increase of SOD activity and GSH level are dependent of endogenous sulfhydryls.

桔梗이 人體 肺癌細胞에 미치는 영향에 대한 실험적 연구

이성열,김원일,박동일 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.4

Platycodi Radix, the root of Platycodon grandiflorum, commonly known as Doraji, is used as a traditional oriental medicine. Extracts from the roots of P. grandiflorum have been reported to have wide ranging health benefits. In the present study, we investigated the effects of an aqueous extract from the roots of P. grandiflorum (AEPG) on the growth of human lung carcinoma A549 cells. results obtained are as fellow; AEPG treatment resulted in the inhibition of the cell viability of A549 cells in a concentration-dependent manner. Upon treatment with AEPG, A549 cells developed many of the hallmark features of apoptosis, including condensation of chromatin. Flow cytometry analysis confirmed that AEPG increased populations of apoptotic-sub G1 phase. Western blot and RT-PCR analyses indicated that the expressions of Bcl-2 was down-regulated but Bax was up-regulated in AEPG-treated A549 cells. AEPG-induced apoptotis of A549 cells was associated with proteolytic cleavage and activation of caspase-3, release of cytochrome C from mitochondria into cytosol and down-regulation of Akt and phospho-Akt proteins in a dose-dependent manner. Induction of apoptosis by AEPG treatment was associated with inhibition and/or degradation of apoptotic target proteins such as poly(ADP-ribose) polymerase, β -catenin and phospholipase C-γ1. AEPG treatment inhibited the levels of cyclooxygenases protein of A549 cells, which was associated with the inhibition of prostaglandin E2 accumulation in a concentration-dependent fashion. Taken together, these findings suggest that P. grandiflorum has strong potential for development as an agent for prevention against human lung cancer.

단삼이 활성산소에 의하여 손상된 배양 해마신경세포에 미치는 영향

이병찬,한선희,송인영,이강창 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.4

In order to evaluate the cytotoxic effect of reactive oxygen species(ROS), the cell viability was measured by MTT assay after cultured mouse hippocampal neurons were treated with various concentrations of xanthine oxidase(XO) and hypoxanthine (HX) for 5 hours. And also, the protective effect of Salviae Mutiorrhizae Radix(SMR) on XO/HX-induced neurotoxicity was examined in these cultures. XO/HX significantly decreased cell viability in dose. and time dependent manners when cultured mouse hippocampal neurons were treated with 5 ~ 40 mU/ml XO for 5 hours. In the protective effect of SMR, SMR increased cell viability dose-dependently after cultured mouse hippocampal neurons were preincubated with 3O ~ 12O ug/ml SM R for 2 hours. From these results, it is suggested that XO/HX is toxic on cultured mouse hippocampal neurons, and herbe medicine such as SMR is very effective in blocking the cytotoxicity induced by ROS.

蘇子降氣楊이 면역반응에 미치는 효과

조현우,신우진,감철우,박동일 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.4

In order to investigate the effect of Sojagangki-tang on imuno-activity, the author performaed this experimental study. Delayed type hypersensitivity (DTH) and rosette forming cells (RFC) for cell-mediated immune response, hemagglutinin (HA) titers, hemolysin (HL) titers for humoral immune response, Carbon clearance for phagocytic function of MPS (mononudear phagocyte system) were measured in ICR mice. The results were summurized as follows: Delayed tape hypersensitivity was increased with statistical significance in the administrated solid extract of Sojagangki-tang treated group as compared with the control group. Hemagglutinin titers were increased with statistical significance in the administrated solid extract of Sojagangki-tang treated group as compared with the control group. Hemolysin titers were increased with statistical significance in the administrated solid extract of Sojagangki-tang treated group as compared with the control group. Number of RFC was increased with statistical significance in the administrated solid extract of Sojagangki-tang treated group as compared with the control group. Carbon clearance was increased with statistical significance in the administrated solid extract of Sojagangki-tang treated group as compared with the control group. Through in vivo experimental study in ICR mice Sojagangki-tang enhences the cell-mediated immmune response, the humoral immune respose. According to the above results, I think that Sojagangki-tang could be used for allergy asthma and lung damage patients.

遠志와 石菖蒲 混合抽出液의 pCT105로 유도된 신경세포암 세포주에 대한 항치매 효과

이성률,강형원,김상태,류영수 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.4

Numerous lines of evidence indicate that some of the neurotoxicity associated with Alzheirner's disease (AD) is due to proteolytic fragments of the amyloid precursor protein (APP). Most research has focused on the amyloid p (AP). However, the possible role of other cleaved products of APP is less clear. Lately It has been reported that a recombinant carboxy-terminal 105 amino acid fragment (CT105) of APP induced strong nonselective inward currents in Xenopus oocyte. In a brain with Atheirner's disease (AD), to investigate the roles of carboxyl-terminal fragment (CT105) of amyloid precursor protein (APP) in apoptosis processes possibly linked to neurodegeneration associated with AD, we examined the effects of the CT of APP with 105 amino acid residues (CT105) on the alteration of apoptosis triggers in neubroblastoma cells. We have investigated whether Radix Polygalae and Rhizoma Acori Grammei mixture extract (RP+RAG) inhibits CT1O5-induced apoptosis of neuroblastoma cells. We found that RP+RAG inhibits CT105-induced apoptosis in SK-N-SH cel!s. Treatment of the cells with RP+RAG inhibited CT105-induced DNA fragmentation and Tunel assay of nuclear chromatin and inhibited the caspase-3 expression in SK-N-SH cells. As the result of this study, In RP+RAG group, the apoptosis in the nervous system is inhibited, the repair against the degerneration of neuroblastoma cells by CT105 expression is promoted. These results indicate that RP+RAG possess strong inhibitory effect of apoptosis in the nervous system and repair effect against the degeneration of neuroblastoma cells by CT105 expression

복분자 미숙과 및 성숙과가 생쥐의 복강 Macrophages에 미치는 영향

이택열,김대근,소준노,권진,송정모,은재순 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.4

The purpose of this research was to investigate the effects of unripened fruits and ripened fruits of Rubus coreanus Miquel on murine peritoneal macrophages. The 70% ethyl alcohol extracts (20 or 100 mg/kg) of unripened fruits (RCE-I) and of ripened fruits (RCE-II) were administered p.o. once a day for 7 days to mice. RCE-II and RCE-II decreased the phagocytic activity of murine peritoneal macrophages and the production of nitric oxide. Also, RCE-I and RCE-II increased the production of tumor necrosis factor. a from peritoneal macrophages. In general, the immuno-suppressive action of RCE-I on macrophages was more potent than those of RCE-II. These results suggest that the fruits of Rubus coreanus Miquel regulates the non-specific immune response via decrease of phagocytic activity and increase of production of tumor necrosis factor-α from murine peritoneal macrophages.

사상체질별 약물의 항당뇨효능에 관한 실험적 연구

이영옥,정성일,조훈석,김정인,임화재,김정상,김종원 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.4

The purpose of this research was to investigate inhibitory activity of Sasang medicines on diabetes. Reduce 36 kind of medicines to powder, abstract add 100 times methanol. Examine inhibitory activity on α-amylase and α-glucosidase in vitro. Thirty six oriental herbs were screened for inhibitory activities against carbohydrate digesting enzymes in vitro. Methanol extract of Pleuropterus mulitiflorus Turez, Ephedra sinica, Moutan cortex Radicis, Magnolia obovata, Alpinia officinarum, Amomi Semen, Rubus coreanus, and Cinnamomi cortex inhibited yeast α-glucosidase activity and extract of Moutan cortex Radicis and Rubus coreanus inhibited porcine α-amylase activity by more 50%. Rubus coreanus showed the strongest inhibition against both α-glucosidase and α-amylase. Therefore, Rubus coreanus was the most effective medicice of 36 kind of medicines.

慢性前立腺炎에 대한 加味敗醬地黃湯의 임상적 효과

손기정,박양춘,김철중 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.4

The aim of this study was to evaluate the effect of Kamipaejangjihwang-tang on chronic prostatitis. Fifty six patients with chronic prostatitis were enrolled this study. We administerd Kamipaejangjihwang-tang to patient during 45 days. Before and after treatment we measured NIH-CPSl(NIH chronic prostatitis symptom score index). After treatment NIH-CPSI was decreased significantly. This result indicated Kamipaejangjihwang-tang be useful for treatment of chronic prostatitis.

XO/HX에 의해 손상된 배양 심근세포에 대한 枳實해백桂枝湯 과 구성약물 추출물의 방어효과

장승호,권강범,김인수,강길성,김인규,김인섭,류도곤 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.4

To certify the protective effect of herbal medicine against oxygen free radical-induced myocardiotoxicity, cytotoxicity was measured using LDH activity and TBARS assay in the presence of Jisilhaebaekgyejitang(JHGT) extracts or single constituents of this prescription. In the present study, xanthine oxidase/hypoxanthine (XO/HX) resulted in a cell damage such as increases in LDH activity in culture medium and lipid peroxidation in cultured myocardial cells. In the effect of JHGT extract and its singe constituents, which are Fructus Ponciri Seu Aurantii lmmaturus (FPSAl), Cortex Magnoliae Officinalis (CMO), Bulbus Allii Macrostemi (BAM), Ramulus Cinnamomi (RC) and Fructus Trichosanthis (FT), they showed the prevention from the XO/HX-induced cardiotoxicity by the decrease of LDH activity and lipid peroxidation. From these results, they show that XO/HX is cardiotoxic in cultured myocardial cells derived from neonatal rat, and it suggests that JHGT, FPSAl, PT, CMO, BAM, RC and FT extracts are positively effective in the blocking in XO/HX-induced cardiotoxicity.

혈관신생 및 이식암세포증식 억제를 통한 가미소암산의 항암작용연구

윤성찬,안성훈,문연자,김진강,추영국,정규용,김영목,우원흥 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.4

Gamisoamsan is a prescription originated in Soamsan which is known as an anti-cancer remedy in the traditional Korean Medicine. To enhance the synergic effects of anti-cancer activity of Soamsan, this study reconstituted the original components of Soamsan with a slight modification and produced a novel herbal remedy, namely Gamisoamsan. To investigate the effects of Gamisoamsan on anti-cancer reaction, I studied the effects of Gamisoamsan on angiogenesis via chorioallantoic membrane (CAM) assay, corneal neovascularization assay and the effects on expression of growth factor which are VEGF, TGF-β, bFGF and IMUP-1. Anti-cancer effects of Garnisoamsan was also abserved through hematological parameters, tumor volume and survival rate in mice. Gamisoamsan inhibited embryonic angiogenesis of blood vessels in CAM assay and inhibited neovascularization of rat cornea. Gamisoamsan reduced cell proliferation in HT1080 cells and IC50 was 2.18 mg/ml. Gamisoamsan reduced the expression of VEGF, TGF-β, bFGF and IMUP-1 which was known as vascular growth factor and this effects of Gamisoamsan was predominant than VP-16. The treatment of Gamisoamsan decreased the CT-26 cell inoculated-tumor volume in mice colon adenocarcinoma and increased mice survival which was inoculated CT-26 cells. The results of the present study suggest that Gamisoamsan extracts has a potential anti-tumor activity and may be an useful remedy to prevent and/or treat cancer.