Human Immunodeficiency Virus Type Ⅰ에 대한 음나무 추출물의 억제활성

Yu Young Beob,Shim Bum Sang,Ahn Kyoo Seok,Choi Seung Hoon,Park Jong Cheol,Miyashiro H.,Hattori M. 대한동의생리학회 2004 동의생리병리학회지 Vol.18 No.4

For the purpose of developing new anti-HIV agents from natural sources, the extracts of Kalopanax pictus were tested for their inhibitory effects on HIV-1 replication and its essential enzymes as the reverse transcriptase (RT). protease and α-glucosidase. In the assay of HIV-1-infected human T-cell line, water extracts of stem and leafstalk inhibited the HIV-1-induced cytopathic effects with Ie (inhibitory concentration) of 25 and 50㎍/㎖, respectively. Moreover water extracts (100㎍/㎖) of stem and leafstalk showed strong activity of 80% and 90% on anti-HIV-1 RT using Enzyme Linked Oligonucleotide Sorbent Assay (ELOSA) method. In the HIV-1 protease inhibition assay, aqueous stem extract inhibited the activity of the enzyme to cleave an oligopeptide, resembling one of the cleavage sites in the viral polyprotein which can only be processed by HIV-1 protease with 58%, but no glucosidase inhibitory activities. We found out this result, for these samples it is possible that the inhibition of the viral replication in vitro is due to the inhibition at least one of RT and protease. It would be of great interest to identify the compounds which are responsible for this inhibition, since all therapeutically useful agent up to date are RT, PR and α-glucosidase inhibitors.

附子中毒으로 유발된 心因性 Shock의 치험 1例

이언정,김형균,신선호,김동웅,신학수,양재훈,최진영,한명아,한상칠,최우정 대한동의생리학회,대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.6

Aconitum carmichaeli Debx(附子) is one of widely used herbal. But, sometimes toxicosis such as general and local paralysis, falling of blood pressure, vertigo, cramp, nausea, vomitting, difficulty of breathing is arised from carelessness in use. This paper is about one patient of acute cardiac shock caused by Acontitum carmichaeli Debx poisoning who admitted to Department of Internal Medicine, Wonkwang University Oriental Medicine Hospital in Jeonju. We treated by means of oriental medicine, such as Gamdutanggamibang(甘豆湯加味方), Saengmaegsangamibang(生脈散加味方), Sophorae Radix (苦蔘), sufficient fluid theraphy, Dopamine injection and O_2 supply. We hope that this case report could help to wide the territory of oriental medicine and more systemic effort could be bone for treatment of acute cardiac Shock caused by Aconitum carmichaeli Debx poisoning.

『동의보감』의 '신체관'과 '질병관'에 관한 연구(1) : 『동의보감』에서 본 생명관 View of the life in『Donguˇibogam』

정우열 대한동의생리학회,대한동의병리학회 2002 동의생리병리학회지 Vol.16 No.1

The development of life science has given many insights into life phenomenons to mankind. Nevertheless, many questions for various phenomenon in life has not been satisfied. Recently, scientific development in human's knowledge showed the limitation of science comparing with the variety of life phenomenon. The fundmental reason of the limitation is thought that the subject of study in life science has been material, and so life has been thought a matter of material in life science. The thought of Hu Jun about this problem, the nature of living things is an important interest in Korean Traditional Medicine, Haneuihak(韓醫學). In this study, the view of human body and disease of Hu Jun(許浚) about the nature of living things which was appeared in『Donguˇibogam』was illuminated. The content of『Donguˇibogam』was edited in the order of Naekyung(內景篇; internal part of the body)·Ouihyung(外形篇; external part of body)·Jabbyung(雜病篇; diseases)·Tangaek(湯液編; medicinal decoction)·Chimgu(針灸篇; acupuncture and moxibustion). The part of Naekyung(內景篇; internal part of the body) is thesis about basic theories and his thought about human body and life, which was seen in 'Shinhyungjangbu-do(身形臟腑圖; figure of body and shape, and the organs and viscera)'·the part of 'Jeong(精)'·Gi(氣)'·'Sin(神)' theory. In this study, the parts which were related with his view of life were selected and compared with the China philosohy of those days to elucidate what is his thought of life appeared in『Donguˇibogam』.

頭位변환에 따른 회전성 현훈증에서 澤瀉湯加味方 투여 20例의 임상고찰

이은,박병욱,고흥 대한동의생리학회,대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.6

This study is to investigate the effect of Taksa-tang(Zexie-tang) on positional vertigo patients. we examined the degree of vertigo and divided 5 grade. In addition we made a diagnosis of vertigo's origin by clinical symptoms, brain-CT and nystagmus test. we divided patients two groups (A and B). A group(11 patients) was taken Taksa-tang (Zexie-tang)(three times a day), Dix-Hallpike maneuver, acupuncture(one time a day) and cupping (one time a day). B group(9 patients) was only taken Taksa-tang(Zexie-tang(three times a day). After treatment, 2 month later we rechecked the degree of vertigo, reattack and remainder symptoms in two groups. Benign positional paroxysmal vertigo was present in 85%, structural lesion of the cerebellum was founded in 10%. In A group, disappearance of symptoms was founded in 64%, improvement was founded in 36%, reattack was founded in 18%. On the contrast, in B group, disappearance of symptoms was founded in 44%, improvement was founded in 33%, reattack was founded in 22% and no response was founded in 11%. Taksa-tang(Zexie-tang) was efficacious against typical features of benign positional paroxysmal vertigo(BPPV) in the acute stage.

『금궤玉函經』에 관한 문헌적 연구

염용하,하기태,김준기,최달영 대한동의생리학회,대한동의병리학회 2002 동의생리병리학회지 Vol.16 No.1

Jinguiyuhanjing, one of the Jin-Yue's three writings which were proofread by Lin-Yi et al. in the Song dynasty, can be regarded as the book composed of another text of Shanghanlun(傷寒論). The book has been quoted by some medical scholars in the Song and Jin dynasty, but it has been kept hiding by some collectors from the Yuan dynasty and has not been known to the public until republished by Shi-Jie Chen in the early Ching dynasty. As compared with Shanghanlun, Jinguiyuhanjing shows much difference in contents. Rather, it has a close similarity to Qianjinyifang(干金翼方) written by SunSaiMiao(孫思邈) in the Dang dynasty. The section 1 of Zhengzhizongcongli(證治總例), which may have edited by the man who have also edited Jinguiyuhanjing published between the Nanbei(南北) dynasty and the Sui(隋) and Tang(唐) dynasty, has a lot of resemblance to Qianjinyifang. Though the book and Qianjinyifang might have come from the same version, it seems that the edition and publication of Jinguiyuhanjing have no direct relation to those of Qianjinyifang because the former has more articles and prescriptions than the other has. Jinguiyuhanjing gives a great deal of attention to scholars who study the taxt of the Song dynasty because the contents of the book particularly reveals a considerable difference to Shanghanlun published in the Song dynasty. Despite all the importances that the book have, however, no one in Korea did not pay attention to the book ever before. We are sure that it is a valuable work to introduce and study the book in Korea.

메틸수은으로 손상된 골모세포에 대한 홍와의 효과에 관한 연구

민부기,홍기연,오연균,신용일,한선희,이상복,신민교,전병훈,송호준,류도곤,박승택 대한동의생리학회,대한동의병리학회 2002 동의생리병리학회지 Vol.16 No.4

To evaluate the osteotoxic effect of methylmercuric chloride(MMC) on cultured mouse osteoblasts, cytotoxic effect was measured by MTT assay after cultured mouse osteoblasts were incubated with various concentrations of MMC for 20 hours. The protective effect of Flos Carthami(FC) against MMC-induced osteotoxicity was also examined in these cultures. MMC decreased cell viability of cultured mouse osteoblasts remarkably in a dose- and time-dependent manners. In protective effect of FC was remarkably effective in blocking the osteotoxicity induced by MMC. From aboved the results, it is suggested that MMC induce osteotoxicity, and the selective herba extract such as FC is very effective in blocking MMC-mediated neurotoxicity on cultured mouse osteoblasts.

枾葉 추출물이 카드뮴에 흡입폭로된 랫드의 독성에 미치는 영향

강성호,천병렬,김상덕,송용선,이기남,정재열 대한동의생리학회,대한동의병리학회 2002 동의생리병리학회지 Vol.16 No.1

Experimental animals were divided into 5 groups; normal, cadmium control, and 3 experimental groups. Cadmium control and experimental groups were exposed to 1㎎/㎥ of cadmium aerosol in air by inhalation exposure for 6 hours/day, 5 days/week during 4 weeks. Dosages of 20, 40, and 80㎎/㎏ of extracts of persimmon leaves were intraperitoneally injected to experimental groups respectively and several toxicological parameters and induction of metallothionein were measured from the rats that inhaled cadmium aerosol in air. The results of this study were as follows. Cadmium concentration that cadmium control and experimental groups were inhaled was 0.980±0.061㎎/㎥. Mass median diameter of cadmium aerosol for inhalation exposure was 4.93±0.483㎛. Cadmium content of normal group in lung was 0.088㎍/g and the highest cadmium content in lung, 55.492㎍/g was from 80㎎/㎏ dose group. Cadmium concentration of normal group in blood was 0.348㎍/100㎖ and the highest cadmium concentration in blood, 2.642㎍/100㎖ was from cadmium control. Cadmium concentration of normal group in liver was 0.010㎍/g and the highest cadmium concentration in liver, 31.100㎍/g was from 20㎎/㎏ dose group. Cadmium concentration of normal group in kidney was 0.030㎍/g and the highest cadmium concentration in kidney, 2.526㎍/g was from cadmium control. Cadmium concentration of normal group in intestine was 0.064㎍/g and the highest cadmium concentration in intestine, 0.300㎍/g was from 80㎎/㎏ dose group. The highest cadmium concentration in urine by week was 6.080㎍/day from 20㎎/㎏ dose group in the fouth week and the highest cadmium concentration in feces by week was 341.731㎍/day from 20㎎/㎏ dose group in the fouth week. Metallothionein concentration of normal group in lung was 5.769㎍/g and the highest in lung, 30.986㎍/g was from 80㎎/㎏ dose group. Metallothionein concentration of normal group in liver was 38.856㎍/g and the highest in liver, 169.378㎍/g was from 40㎎/㎏ dose group. Metallothionein concentration of normal group in kidney was 22.228㎍/g and the highest in kidney, 47.898㎍/g was from 80㎎/㎏ dose group. Metallothionein concentration of normal group in intestine was 2.170㎍/g and the highest in intestine, 13.642㎍/g was from 80㎎ dose group.

加味參朮三子湯의 抗癌活性에 관한 연구

김성언,이효정,이효정,김동희,송규용,김성훈 대한동의생리학회,대한동의병리학회 2002 동의생리병리학회지 Vol.16 No.4

The purpose of this study was to investigate the effect of Kamisamchulsamja-tang(KSST) water extract on the antitumor activity. The results were summarized as follows: KSST extract exhibited a weak cytotoxicity against HT1080, A549, SK-OV-3, B16-F10 and SK-MEL-2 cells. KSST extract showed a inhibitoty effect on DNA topoisomerase Ⅰ from calf thymus in a dose-dependent manner. Also, KSST extract showed antiadhesive effect on HT1080 cells but didn't showed on A549 cells to complex extracellular matrix. In pumonary colonization assay, a number of colonies in the lungs were decreased significantly in KSST treated group as compared with control group. In vitro neovascularization assays, angiogenesis was significantly inhibited in KSST treated group than control group. In CAM assay, KSST extract inhibited angiogenesis significantly at 15㎍/egg concentration as compared with control. From the above results it was concluded that KSST showed antitumor effect through the antimetastatic effect. So it is expected to be clinically helpful on the prevention of metastasis of cancer.

여로의 抗菌作用에 관한 연구

지규용 대한동의생리학회,대한동의병리학회 2002 동의생리병리학회지 Vol.16 No.1

This study was carried out to investigate the efficacy of antifungal and antibacterial activity of Veratri Herba, which was used as insecticide in the clinical part of oriental dermatology for a long time. Sample herb was selected with Veratrum maackii in the middle of 5 veratrum species. The experimental results of this herb were as follows. Crude extract showed various relative inhibitory rates from 68.9% to 95.6% in 2㎎/disc against Fusarium oxysporum, Botrytis cineria, Saccharomyces cerevisiae, Escherichiae coli, and Bacillus subtilis on the basis of inhibitory range against B. cineria, and the inhibitory effect was decreased depending on density. In the extract of various organic solvent, chloroform and ethyl acetate fraction show the various size of clear zone against all microbes. And in the subfraction assay from ethyl acetate fraction, crude alkaloids show the antibacterial and antifungal effect significantly.

중풍 환자의 혈압 변화에 관한 임상적 고찰

권정남,김영균,최문경 대한동의생리학회,대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.6

Paralysis is a neurological disease which suffers numerous people around the world as well as Korea. It is one of the main three causes of death. The two others are cancer and hearth disease. To make matters worse, particularly, paralysis ranks top in the list of death causes of Korean people. This researcher randomly sampled and analyzed medical records of 467 patients who was hospitalized and treated at Chinese Medicine hospital of Dongeui university after they were diagnosed as having cerebral hemorrahage through Brain CT and Brain MRI photography. The researcher checked those paralyzed patients from entering till leaving the hospital in regard to their high blood pressure distribution, average blood pressure, whether they took blood pressure medicines or not, and variations in blood pressure in accordance with the time period of hospitalization and whether or not the patients took medicines treating blood pressure. In terms of high blood pressure, 283(60.6%) patients among the total surveyed in this study were found having high blood pressure provided considered based on the contraction stage of blood pressure, while 257(55%) was under an equivalent status based on the relaxation stage of the same measure. Those who suffered from high blood pressure were occupied by 102 men, 59% of the total same sex, 172, and 181 women, 61% of the total equivalent sex, 297, when evaluated based on the contraction stage mentioned above while, 90 men and 167 women, the relaxation stage. Among all of the patients with high blood pressure, that is 283(contraction stage)and 257(relaxation stage), 1(0.4%) and 2(0.8%) were respectively at their 30s, 19(6.7%) and 18(7.0%) at their 40s and 49(17.3%) and 39(15.2%) at their 50s. 111(39.2%) and 107(41.6%) 87(30.7%) and 78(30.4%), and 16(5.7%) and 13(5.1%) respectively at their 60s, 70s and 80s. These indicate that an absolute majority of high blood pressure patients was occupied by those at their 60s and 70s. When all of the 467 patients were classified in terms of hospitalization period, 201 patients (43.04%) belonged to <1 Week's Group>, followed by 140(29.98%), <2 Weeks' Group>, 82(17.56%), <4 Weeks'group> and 44(9.42%), <8 Week's Group>. Categorized in accordance to whether they had took any medicines to control blood pressure at the time of entering hospital, the total subject was divided into A,B and C groups. Out of 467 patients, in detail, 191(40.9%) became under <Group A>, 215(46.4%), <Group B> and 61(13.06%), <Group C>. For all the patients selected for the study, mean blood pressure was 153.7/94.4 when they were hospitalized and 134.6/85.3 when they left hospital. In <Group C> whose 61 members began to take blood pressure medicines during hospitalization, average blood pressure significantly decreased to 148.0/93.1 after taking the medicines from 164.3/101.3 before the treatment. Consequently, the level of blood pressure in the part of the whole patients under survey here was significantly changed or decreased after they received proper treatments and according to the time period of their hospitalization.