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Inhibitory Effects of Herb and Seeds of Oenothera odorata on the Melanogenesis
최미은,우원홍,문연자,Choi, Mi-Eun,Woo, Won-Hong,Mun, Yeun-Ja The Society of Korean Medicine Ophthalmology 2010 한방안이비인후피부과학회지 Vol.23 No.1
목적 : 월견초는 다량의 불포화 지방산인 리놀렌산과 감마 리놀렌산을 함유하고 있으며, 천식성 기침이나 아토피피부염에 효능이 있는 것으로 알려져 있다. 본 연구는 월견초의 전초와 종자 추출물의 피부 멜라닌 합성에 대한 억제 효과를 조사하였다. 방법 : B16F10 멜라닌세포주를 이용하여 멜라닌, tyrosinase 활성 및 세포생존율을 측정하였다. 또한 멜라닌 합성- 관련효소인 tyrosinase, TRP-1, TRP-2의 단백질발현과 $\alpha$-MSH를 처리하여 색소침착을 유도 한 뒤 단백질 발현을 조사하였다. 결과 : 월견자는 B16F10 세포의 멜라닌 합성을 $5\;{\mu}g/ml$와 $10\;{\mu}g/ml$ 농도에서 각각 대조군의 81.3%, 68.3%로 억제하였고 tyrosinase의 활성도 이와 유사하게 억제하였다. 멜라닌 합성-관련효소들의 단백질발현을 관찰한 결과 월견초와 월견자는 tyrosinase 발현을 억제하였으며 TRP-1과 TRP-2의 발현에는 영향을 주지 않았다. 특히 $\alpha$-MSH에 의한 과색소 유도 시 tyrosinase 발현이 현저하게 감소되었으며, 월견자의 멜라닌 합성 억제 효과가 월견초 보다 높게 나타났다. 결론 : 이상의 연구 결과 월견자는 멜라닌세포의 tyrosinase 단백질 발현과 tyrosinase 활성을 억제하여 멜라닌 생성을 감소시키는 것으로 사료된다.
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보양환오탕에 의한 비특이적 세포독성 T 세포 활성 증강
하종천,김영현,우원홍,남상윤,Ha, Jong-Cheon,Kim, Young-Hyun,Woo, Won-Hong,Nam, Sang-Yun 한국생약학회 2001 생약학회지 Vol.32 No.3
To explore the possible cancer therapeutic application of "Bo-yang-hwan-oh-tang" (BH), a herbal medicinal recipe used for improvement of blood stasis, we have examined its direct cytotoxicity against tumor cell, and induction of cytotoxic activity of lymphocytes. Water extract of BH alone did not exhibit direct cytotoxicity to Yac-1 target cells even with high concentrations (10 mg/ml). By exposure for 3 days, BH did not induce any nonspecific cytotoxic activity of mouse spleen cells, either, when assessed in a 4 hr $^{51}Cr-release$ assay. However, when BH was added during CD3 stimulation of non-adherent spleen cells, non-specific CTL activity was markedly promoted in a dose dependent manner. In contrast, BH did not alter activated NK cell activity following IL-2 stimulation. These data suggest that BH does not induce but upregulates non-specific CTL effecter function and that activated NK cell does not respond to BH. For elucidation of the mechanism underlying this function of BH, time kinetic study for IL-2 production using ELISA was undertaken. IL-2 production following CD3 stimulation was significantly augmented and higher level of IL-2 is sustained over 3 days in the culture medium by BH treatment. Moreover, addition of exogenous IL-2 during CD3 stimulation resulted in a similar level of cytotoxicity between control and BH-treated culture. These data indicate that the BH-mediated upregulation of non-specific CTL activity is contributed by augmentation of IL-2 production. Our data imply the possible application of BH for combination therapy of cancer with non-specific activator.
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박인해,홍석훈,우원홍,문연자,차수빈,Park, In-Hae,Hong, Seok-Hun,Woo, Won-Hong,Mun, Yeun-Ja,Cha, Su-Bin 대한한방안이비인후피부과학회 2017 한방안이비인후피부과학회지 Vol.30 No.4
Objectives : This Study was conducted to investigate the melanin synthesis effect of Eclipta prostrata and to determine the relationship between melanin synthesis effect of Eclipta prostrata and cAMP/PKA melanin synthesis pathway. Methods : We measured melanin contents, tyrosinase activity, expression of TRPs, p-CREB in B16F10 cells cultured with Eclipta prostrata ethanol extracts(EEP). And after treatment with H89 and dibutyryl cAMP, which inhibit or promote the activation of PKA, we observed changes in melanin synthesis and tyrosinase activity stimulated by EEP. Results : EEP increased melanin synthesis by promoting the expression of tyrosinase and TRP-1. It also promoted expression of p-CREB. H89 suppresses melanogenic effect and expression of tyrosinase, TRP-1 in B16F10 stimulated by EEP. Dibutyryl cAMP promotes melanogenic effect of EEP. Conclusions : The results of this study suggest that melanin synthesis effect of EEP is related to induction of tyrosinase and TRP-1 expression through the cAMP/PKA pathway.
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김수경,최형욱,우원홍,Kim, Soo-Kyung,Choi, Hyung-Wook,Woo, Won-Hong 대한예방한의학회 2015 대한예방한의학회지 Vol.19 No.2
Objective : This study was a covariance structural analysis to identify korean medicine health promotion behavior by the general characteristics of the subjects and build a predictive model and theoretical framework based on Pender's health promotion model(1996) and related literature reviews. Method : A hypothetical model was consisted of 8 theoretical variables and 27 measured variables. Related variables included Individual Characteristics and Experience, Behavior-specific cognitions and affect and Behavioral outcome. The data was collected from 802 middle and old-aged people living in Seoul and Gyeong gi province through structured questionnaires by face to face interviews between February and March, 2014. SAS ver. 9.1 and AMOS 18.0 programs were used for the data analysis. Results : Difference in the verification of Korean medicine health promotion behavior by the general characteristics, Older people who are male, with higher economic status, no chronic disease or with diabetes, no smoking, no drinking, with more exercise showed significantly higher scores, but education level has no difference. 15 paths were statistically significant among 16 paths on the direct effect, 6 paths were statistically significant among 9 paths on the indirect effect in the hypothetical model. The greatest impact variable on Korean medicine health promotion behavior was perceived self-esteem. Also, the findings showed that the higher perceived social support, perceived health status, previous Korean medicine health promotion behavior, community environment, perceived benefit and the lower perceived barrier had a significant effect on Korean medicine health promotion behavior. Conclusion : This research model has an empirical validity as the variables of this study verified their effects and significances. Therefore, the understanding of Korean medicine health promotion behavior can be increased and the utilization will be higher when seeking a comprehensive health promotion plan. Also, a strategy can be utilized the strategy for Korean medicine health promotion behavior.
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싸리꽃 에탄올 추출물의 피부(皮膚) 생리활성(生理活性) 효과
유인식,이성진,이성원,문연자,우원홍,김영목,이장천,임규상,Ryu, In-Sik,Lee, Seon-Jin,Lee, Sung-Won,Mun, Yeun-Ja,Woo, Won-Hong,Kim, Yeong-Mok,Lee, Jang-Choen,Lim, Kyu-Sang 대한한방안이비인후피부과학회 2007 한방안이비인후피부과학회지 Vol.20 No.2
Objective : In this study, the ethanol extract from flowers of Lespedeza bicolor was investigated for their dermal bioactive properties related to cosmeceuticals such as depigmentation and radical scavenging effect. Results : The ethanol extract from flowers of Lespedeza bicolor showed considerable radical scavenging activity ($SC_{50}:\;10\;{\mu}g/ml$) and inhibited the production of nitric oxide (NO) in Raw 264.7 macrophages activated with the endotoxin lipopolysaccharide (LPS). Although the proliferation of B16/F10 cells was slightly decreased by the ethanol extract from flowers of Lespedeza bicolor at the concentration of $100\;{\mu}g/ml$, it did not appear necrosis. The ethanol extract from flowers of Lespedeza bicolor down-regulated melanin formation effectively. Methods : The free radical scavenging activity of Lespedeza bicolor was assayed in cell free systems using a stable free radical, 1,l-diphenyl-2-picrylhydrazyl (DPPH). Nitrite accumulated in culture medium was measured as an indicator of NO production using a Griess reaction. Cell viability was measured by MTT assay and melanin content was assessed using the method of Hosei with some modifications. Conclusions : These results suggest that the ethanol extract from flowers of Lespedeza bicolor is a potent depigmetation agent and it may be a candidate for antioxidant and anti-inflammatory agent.
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이성진,김대성,문연자,우원홍,이장천,임규상,Lee, Sung-Jin,Kim, Dae-Sung,Mun, Yeun-Ja,Woo, Won-Hong,Lee, Jang-Cheon,Lim, Kyu-Sang 대한한방안이비인후피부과학회 2009 한방안이비인후피부과학회지 Vol.22 No.1
Objective : The purpose of this study is to investigate the antibacterial activity of Saussurea lappa for the applications of herb-based extracts to both cosmetic and medicinal industries. Methods : Antibacterial activity of ethanol extract of Saussutes lappa (ESL) was assessed using agar diffusion and broth dilution methods, and determined by whether clear zone was formed around paper disc and in terms of the size (mm) of clear zone, Results : ESL provided activity against Staphylococcus aureus KCTC 1927 in concentration of 1 mg/ml with an clear zone of 16 mm, and showed an activity against Staphylococcus epidermids KCTC 1917 in concentration of 1 mg/ml with an clear zone of 18mm. Moreover, the minimum inhibitory concentration (MIC) of ESL against S. sureus and S. epidermidls were 1 mg/ml for both bacterial species. However, ESL showed no growth inhibition against Pseudomonas aeruginosa KCTC 12513, Enterobactet aerogenes KCTC 2190, Escherichia coli KCTC 2571, Salmonella typhimurium KCTC 1925 and Propionibacterium acnes KCCM 41747. Conclusions : Antibacterial activity of ESL against Staphylococcus sureus and Staphylococcus epidermids causing eye and skin diseases was proved. The result suggest that ESL may be useful as a natural preservative on behalf of synthetic preservatives.
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조미경,안병상,문연자,우원홍,Jo, Mi-Gyeong,An, Byung-Sang,Mun, Yeun-Ja,Woo, Won-Hong 대한한방피부미용학회 2005 대한한방피부미용학회지 Vol.1 No.1
The aim of this study was to investigate the effect of Fructus Forsythiae on the melanogenesis. To determine whether Fructus Forsythiae methanol extract suppress melanin synthesis in cellular level, HM3KO human melanoma cells were cultured in the presence of various concentrations of Fructus Forsythiae methanol extract and the effects on cell proliferation, tyrosinase activity and melanin contents were examined. Treatment with Fructus Forsythiae methanol extract inhibited tyrosinase activity, regulate melanin biosynthesis as the key enzyme in melanogenesis, in a dose-dependent manner. And also suppressed melanin contents as a dose dependent manner without cytotoxicity morphological change. It was observed that the color of cell pellets was totally different from the control. These results suggest that the inhibitory effect of Fructus Forsythiae methanol extract on melanogenesis is due to the suppression of tyrosinase in HM3KO cells and Fructus Forsythiae is a candidate for an efficient whitening agent.
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