한국 여성에서 자궁내막증과 GSTM1, GSTT1 유전자 결손 다형성의 연관성

김태준 ( Kim Tae Jun ),김석현 ( Kim Seog Hyeon ),최영민 ( Choi Young MIn ),전종관 ( Jun Jong Kwan ),박성효 ( Park Seong Hyo ),박인애 ( Park In Ae ),구승엽 ( Ku Seung Yup ),서창석 ( Suh Chang Suk ),김정구 ( Kim Jeong Gu ),문신용 ( 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.7

목적 : Phase Ⅱ glutathione S-transferase mu, theta 효소 유전자 (GSTM1, GSTT1) 결손 다형성과 자궁내막증의 연관성을 알아보고자 하였다. 연구 방법 : 복강경술 또는 개복술로 자궁내막증으로 진단된 환자 148명과 복강경술 또는 개복술에서 자궁내막증이 없는 것으로 확인된 대조군 130명을 대상으로 하여, GSTM1 및 GSTT1 유전자의 결손 다형성 여부를 조사하였다. 결과 : 자궁내막증 환자와 대조군 사이에 GSTM1 유전자 결손 다형성 (GSTM1 0/0)의 빈도는 유의한 차이를 보였으나 (61.5% vs 49.2%, p=0.040), GSTT1 유전자 결손 다형성 (GSTT1 0/0)의 빈도는 차이가 없었다 (51.4% vs 54.6%, p>0.1). GSTM1 유전자의 경우 stage Ⅲ-Ⅳ인 중증 자궁내막증과 유의한 상관 관계를 보였으나 stage Ⅰ-Ⅱ인 경한 자궁내막증과는 유의한 차이를 보이지 않았다. 결론 : GSTM1 유전자 결손 다형성과 한국 여성에서 자궁내막증 발생과 연관이 있었으며, GSTT1 유전자 결손 다형성과는 관련이 없었다. Objective : To explore the association of the phase Ⅱ glutathione S-transferase mu (GSTM1), theta (GSTT1) gene null polymorphisms with endometriosis in a Korean population. Methods : One hundred forty eight women with surgically diagnosed endometriosis of stage Ⅰ-Ⅳ were recruited. And 130 patients with no evidence of endometriosis by laparoscopy or laparotomy served as controls. Genomic DNA was extracted from peripheral blood and analyzed for GSTM1, GSTT1 null polymorphisms. Results : GSTM1 null genotype (GSTM1 0/0) was found more frequently in patients with endometriosis than in controls (61.5% in endometriosis vs. 49.2% in control, p=0.040). And the association was found only in moderate to severe endometriosis (stage Ⅲ Ⅳ) (63.7% vs. 49.2%, p=0.027: odds ratio: 1.812), not in minimal or mild endometriosis (p=0.395). There was no significant difference between endometriosis patients and controls in the frequency of the GSTT1 null genotype (51.4% vs. 54.6%, p>0.1). Conclusion : These results suggest that GSTM1 null genotype is associated with the risk of endometriosis but GSTT1 null genotype is not in the Korean population.

심층 신경회로망을 이용한 운전자 조향특성 분류에 대한 연구

류호주(Ho-Ju Ryu),김정구(Jeong Ku Kim),정슬(Seul Jung) 대한전자공학회 2022 대한전자공학회 학술대회 Vol.2022 No.11

In this paper, a classification algorithm for the drivers steering characteristics is presented using a deep neural network(DNN). DNN is composed of convolution neural network(CNN) for feature extraction and fully connected neural network for classification. Actual drivers steering data were collected from a real car and those data were trained by DNN into three classes: ‘strong’, ‘medium’, and ‘weak’ depending upon their forces. The classification accuracy of the algorithm was about 81.2%.

자궁내막증 환자에서 자궁내막의 VEGF 발현 양상

정창원,박인애,홍민아,이경훈,최영민,구승엽,지병철,서창석,김석현,김정구,문신용,Jeong, Chang-Won,Park, In-Ae,Hong, Min-A,Lee, Gyoung-Hoon,Choi, Young-Min,Ku, Seung-Yup,Jee, Byung-Chul,Suh, Chang-Suk,Kim, Seok-Hyun,Kim, Jung-Gu,Moon, Shi 대한생식의학회 2010 Clinical and Experimental Reproductive Medicine Vol.37 No.2

목 적: 자궁내막증 환자군과 자궁내막증이 없는 대조군의 정상 자궁내막에서 생리주기에 따른 vascular endothelial growth factor (VEGF)의 발현 양상과 양 군간의 차이를 살펴, 자궁내막의 VEGF와 자궁내막증 발병의 상관관계를 알아보고자 하였다. 연구방법: 환자군은 자궁적출술을 시행 받은 환자 중 3기 이상의 자궁내막증으로 조직학적 진단을 받고, 자궁내막의 조직학 주기가 확인 가능한 40세 이하의 환자 64명을 대상으로 하였고, 대조군은 같은 시기에 자궁경부내 상피병변으로 자궁적출술을 시행 받은 환자 중 자궁내막증이나 자궁선근증이 없고 자궁내막의 주기가 확인 가능한 40세 이하의 환자 37명이었다. 환자군과 대조군의 정상 자궁내막에서 얻은 조직들을 면역조직화학 염색을 시행하여 반 정량적인 VEGF의 조직학적 점수를 산출하여 두 그룹간에 같은 월경주기 별 차이를 비교하였다. 결 과: 선세포와 기질세포로 나누어 같은 월경주기에 따라 자궁내막증군과 대조군을 비교하였을 때, 증식기와 분비기 모두에서 선세포 및 기질세포의 VEGF의 발현은 차이가 없었다. 각 세분된 월경주기 별 비교에서도 두 군간에 유의한 차이를 발견할 수 없었다. 자궁내막증 환자군내에서는 선세포와 기질세포 모두 분비기에서 증식기보다 높은 VEGF 발현 양상을 보였다. 결 론: 자궁내막증 환자군과 정상 대조군의 자궁내막에 있어서 VEGF 발현 양상은 차이가 없었다. 즉 자궁내막증 환자의 자궁내막에서 VEGF 발현이 정상인에 비해 증가되어 있지 않고 이는 자궁내막의 VEGF 발현은 자궁내막증의 병인생성에 관련이 없을 가능성을 제시한다. Objective: The purpose of this study was to evaluate the correlation between the expression pattern of vascular endothelial growth factor (VEGF) in endometrium and the pathogenesis of endometriosis by investigating VEGF expression patterns and their difference between eutopic endometrium of patients with endometriosis and that of normal controls without endometriosis. Methods: Endometrial sections were obtained from 64 hysterectomy specimens from women under age of 40, who had undergone hysterectomies and had histological evidence of endometriosis, with stage 3 and 4 according to the revised American Society for Reproductive Medicine classification. As for controls, 37 sections were gained from women diagnosed as having cervical intraepithelial neoplasia (CIN) of the uterine cervix and without evidence of pelvic endometriosis or adenomyosis during their operation. The VEGF content was evaluated immunohistochemically in the eutopic endometrium from 64 patients with endometriosis and 37 normal controls. Histological semiquantitative score (H-score) was calculated and compared between study group and control group throughout the menstrual cycle. Results: There was no significant difference in the H-score of VEGF in the eutopic endometrium between patients with endometriosis and controls without endometriosis when compared according to the same phase of the cycle, although the H-score of the study group was significantly higher in the secretory phase than the proliferative phase. Conclusion: The VEGF expression in the eutopic endometrium of women with endometriosis was not different from that of women without endometriosis. This study suggests VEGF expression in eutopic endometrium is unlikely associated with the pathogenesis of endometriosis.

무력정자증 환자에서 인간 난포액과 SpermGrad를 이용한 정자처리법의 비교

정연경,이정렬,문정희,김현준,한상훈,지병철,구승엽,서창석,김석현,최영민,김정구,문신용,Chung, Youn Kyung,Lee, Jung Ryeol,Moon, Jeong-Hee,Kim, Hyun-Jun,Han, Sang-Hoon,Jee, Byung-Chul,Ku, Seung-Yup,Suh, Chang-Suk,Kim, Seok-Hyun,Choi, Young-M 대한생식의학회 2006 Clinical and Experimental Reproductive Medicine Vol.33 No.1

목 적: 보조생식술에서의 정자처리법으로서 인간 난포액을 이용한 swim-down 방법의 유용성을 확인해 보고자 하였다. 연구방법: 분당서울대학교병원 산부인과에서 불임평가 목적으로 정액검사를 시행할 때 정자무력증 (asthenozoospermia, sperm motility < 50%)을 보이는 12명의 남성을 대상으로 하였다. 이들에서 검사 후 남은 정액을 100% 인간 난포액을 이용한 swim-down법과 SpermGrad를 이용한 밀도차 분리법을 적용하여 각각 처리하고 컴퓨터 정액분석기를 이용하여 정액검사를 시행하였다. 결 과: 두 군 모두에서 운동성과 빠른 운동성 정자의 비율, VCL (curvilinear velocity), ALH (amplitude of lateral head displacement)치 및 과활동성 정자의 비율이 통계적으로 유의하게 상승하였고 LIN (mean linearity)치는 유의하게 감소하였다. 100% 인간 난포액을 이용한 swim-down법에서 정자의 운동성이 SpermGrad를 이용한 밀도차분리법에 비하여 유의하게 높게 나타났으며 ($81.2{\pm}4.7$ vs. $67.6{\pm}2.3$, p=0.02), 다른 변수들은 두 군에서 차이를 보이지 않았다. 결 론: 100% 인간 난포액을 이용한 swim-down법은 정자무력증을 보이는 경우 유용한 정자 처리법으로 사료된다. Objective: We tested the usefulness of swim-down technique using human follicular fluid (hFF) in sperm preparation. Methods: Semen samples were obtained from twelve male partners showing asthenozoospermia (sperm motility < 50%) at the time of routine andrologic evaluation in Seoul National University Bundang Hospital. After dividing into two aliquots, each samples were processed either by swim-down using 100% hFF or density gradient using SpermGrad. Sperm quality was assessed by computer-assisted semen analyzer (CASA). Results: Motility, Rapid motility, VCL (curvilinear velocity), ALH (amplitude of lateral head displacement), and hyperactivated sperms were significantly increased, and LIN (mean linearity) was decreased significantly after sperm preparation in both groups. Motility was significantly higher after swim-down using 100% hFF when compared with density gradient using SpermGrad ($81.2{\pm}4.7$ vs. $67.6{\pm}2.3$, p=0.02) The other parameters assessed by CASA were not different between the two methods. Conclusion: Swim-down method with 100% hFF may be a useful method in preparation of sperm from asthenozoospermia.

인간과 로봇간의 움직임의 무선 인터페이스 구현

전풍우(Poong-Woo Jeon),조현택(Hyun-Taek Cho),장평수(Pyoung-Su Jang),조기호(Ki-Ho Cho),김정구(Jeong-Ku Kim),송덕희(Duk-Hee Song),최영권(Young-Kwon Choi),정슬(Seul Jung) 제어로봇시스템학회 2001 제어로봇시스템학회 국제학술대회 논문집 Vol.2001 No.10

한국인 혈우병 A 가계에 있어 Factor Ⅷ 유전자내 Intron 13 및 Intron 22 부위 Microsatellite 다형성 양상 및 이를 이용한 혈우병 A 보인자 진단 및 산전진단

김은주,박성효,김정구,최영민,이진용,전종관,김석현,문신용,황도영,서창석,최진,구승엽 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.5

We have undertaken this study to identify the usefulness of two variable dinucleotide tandem repeats within the factor Ⅷ gene for carrier detection and prenatal diagnosis of hemophilia A in the Korean population. We have analyzed these polymorphisms in 50 unrelated Korean mothers of patients with severe hemophilia A, using polymerase chain reaction. The expected heterozygosity rates of the intron 13 and intron 22 dinucleotide repeats were 56% and 40%, respectively. Analysis of the intron 13 and intron 22 dinucleotide repeats revealed heterozygous patterns in 29(58%) and 17(34%) of 50 mothers studied, respectively. The combined overall informativity of the intron 13 and intron 22 dinucleotide repeats was 68%. Using linkage analysis with the intron 13 dinucleotide repeats, we have attempted three cases of carrier detection and two cases of prenatal diagnosis in two families of patients with severe hemophilia A. Two pregnant women were diagnosed as carriers, and the other patients as non-carrier Prenatal diagnosis revealed an unaffected male in one fetus, and an unaffected female in another fetus. This data demonstrated that the analysis of the intron 13 and intron 22 dinucleotide repeats very useful in the carrier detection and prenatal diagnosis of hemophilia A in the Korean population.

대장내시경 검사 직후 복통 완화를 위한 직장관 사용의 유용성

김호진,장재권,박찬원,박정원,윤종보,김정구,송광수,이인범,조용호,허성호 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.1

Background/Aims: Abdominal distension from the insufflation of air can create more troublesome discomfort after colonoscopy (CFS) than after upper endoscopy. Many patients report difficulty in expelling insufflated air after CFS. One previous study demonstrated that insertion of rectal tube at the conclusion of CFS significantly improves patient satisfaction with the procedure. The aim of this study was to compare the effectiveness of rectal tube placement for abdominal decompression after CFS in an effort to limit patient discomfort by carefully applying air suction during CFS. Methods: We conducted a prospective trial in 103 consecutive patients undergoing elective CFS. Patients were randomized to receive rectal tube placement at the end of the procedure or simple air suction during the procedure. Patients were evaluated by clinical symptoms and signs 10 minutes after completion of CFS. Results: All 103 patients had a complete examination of the colon to the cecum. No specific complications occurred during and a day after examination. The two groups were well matched with respect to age, sex, height, weight, chief complaints. There were no significant differences between the two groups for bowel preparation, duration of examination, abdominal pain during procedure, abdominal disten-sion 10 minutes after CFS, and abdominal pain 10 minutes after CFS (p >0.05). Conclusions: Meticulous air suction during CFS reduces abdominal discomfort and distension after CFS and is as effectively as the placement of a rectal tube at the con-clusion of CFS.

장골동맥 폐쇄를 동반한 뢰플러 심내막염 1예

신상곤,이두하,허성호,김정구,이현택,이홍석,구진하,정태호,이상민,권오준 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.11