Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

산딸나무 열매로부터 저밀도 지질 단백질(LDL) 산화 역제물질의 분리

이대영,송명종,김민정,정낙훈,정태숙,이윤형,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

산딸나무 열매를 100% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography를 반복하여 1종의 lignan 화합물을 분리, 정제하였다. 화합물의 화학구조는 NMR, Ms 및 IR 등의 스펙트럼 데이터를 해석하여, (+)-pinoresinol (1) 로 동정하였다. 이 화합물은 산딸나무에서 처음 분리되었다. LDL-항산화활성 억제활성이 20㎍/mL 처리농도에서 85.1±1.3%로 나타났다. (+)-Pinoresinol was isolated from the EtOAc fraction of methanol extracts of the fruits of Cornus kousa Burg. The chemical structures of (+)-pinoresinol was determined by spectroscopy method, as well as 1D- and 2D-NMR (^(1)H-^(1)H COSY, HSQC and HMBC) spectroscopy. This compound was the first to be isolated from C. kousa Burg. Also, (+)-pinoresinol inhibited LDL-oxidation with the inhibitory activity of 85.1±1.3% at a concentration of 20 ㎍/mL.

Trichoplusia ni 세포의 apoptosis 메커니즘 규명을 위한 기초연구

이종민,정인식,양재명,이윤형 한국농화학회 2001 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.1

To elucidate the apoptosis mechanism of Trichoplusia ni cell, fundamental studies for apoptosis induction and suppression were performed. Hygromycin B, a known inducer of apoptosis, started the inhibition of T. ni cell growth at 200 ㎍/㎖ concentration. Furthermore, at 400 ㎍/㎖ concentration, DNA fragmentation was detected on day 2 of incubation. Although both dexamethasone and sodium butyrate inhibited T. ni cell growth, DNA fragmentation was not detected by both treatments. Also, when apoptosis induced T. ni cells with 200 ㎍/㎖ hygromycin B were treated with caspase inhibitor (Ac-DEVD-CHO), the apoptotsis was suppressed by 36%. In addition, N-acetylcysteine, another apoptosis repressor, also inhibited the apoptosis of T. ni cells. In order to express the anti-apoptosis gene (bcl-2), T. ni cells were transiently transformed with bcl-2 and its expression was confirmed by western blot analysis. These results showed the potential of developing new insect cell lines with suppressed apoptosis.

장상피화생과 Helicobactor pylori와의 상관 관계에 관한 연구

이복희,김대수,이현숙,진영주,윤세진,송형근,성노현,정현용,이동호 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

In 1965 Lauren divided advanced gastric carcinoma into two main types-namely, "intestinal" and "diffuse" type carcinoma, which differ not only morphologically but also in their clinical and epidemiological characteristics. Moreover, a different histogenetic process has been postulated by many authors that intestinal type gastric carcinoma might arise from areas with intestinal metaplasia. Since Warren and Marshall reported the presence of Helicobacter pylori in the stomach in 1983, strong evidence has been obtained that there is a close relationship between Helicobacter pylori and chronic gastritis, atrophic gastritis, intestinal metaplasia, and intestinal type gastric carcinoma. To study the relationship between intestinal metaplasia and Helicobacter pylori infection, we performed gastroscopic biopsies and CLO tests taken from patients with epigastric pain. Only 87 patients with macroscopically suspected antral gastritis were included. The prevalence of intestinal metaplasia in gastric antral biopsies increased from 33.3% in the age group<30 years to 63.6% in the age group 60 years. When all patients were divided into two age groups-namely, (i)<50 years(n=41) and (ii)≥ 50 years(n-=46), intestinal metaplasia was found significantly more often in patients ≥ 50 years. ((i) 39.2%, (ii) 54.3%, p<0.0001). The prevalence of Helicobacter pylori in gastric antral biopsies decreased from 66.7% in the age group<30 years to 62.5%, in the age group in the age group 50 to 59 years. When all patients were divided into the same two age groups-namely(i)<50 years and (ii)≥50 years, the prevalence of Helicobacter pylori did not differ between two groups in a statistically significant way. The mean age of intestinal metaplasia positive patients who were also Helicobacter pylori positive was 48±11.0(mean±S.D.)years, whereas the mean age of intestinal metaplasia positive patients who were Helicobacter pylori negative was 52±9.7(mean±S.D.)years. In conclusion. although our data do not prove a causal relationship between Helicobacter pylori infection and the histogenesis of intestinal metaplasia, we suggest that Helicobacter pylori plays an important role in the development of intestinal metaplasia in the gastric mucosa. Whether Helicobacter pylori has to be present at all times during this process or is only necessary as a 'trigger', needs further research.

수중환경 변수의 변화에 따른 능동소나 표적검출 성능분석에 관한 연구

윤대희,이충용,성하종,박성범,정형성 연세대학교 산업기술연구소 1997 논문집 Vol.29 No.2

In this paper, the performance of active sonar system is analyzed under various underwater acoustic environments using the multipath eigenray model in shallow water. In addition, an approximated shallow-water propagation model is proposed for the active sonar based on MacPherson and Daintith's model and corresponding equations comprising active sonar parameters are derived. To analyze the sonar performance according to the changes of various sonar parameters, a large number of measurements and results in ocean acoustics and digital signal processing are combined. The proposed model is compared with the multi-path eigenray model in terms of the echo level passed through the beamformer, the input SNR of the detector and the detection probability.

성분법에 의한 철골 보-기둥 용접 접합부 관한 초기강성에 대한 연구

이윤신,안형준 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

In design practice welded connections represent the most commonly used connection typology due to their ability to reduce the relative rotation between the beam and the column allowing, in many cases, the simple modelling of the building structure as a continuous (i.e. rigid) frame. The prediction of the behavior of beam-to-column connections can be carried out by means of the component method which is largely applied in research studies. In the finite element analysis, the fillet of H-shaped steel, the welding property, and the effect of remained strain of welding were ignored to simplify the specimens in this study. The purpose of this paper is to analyze the initial stiffness of beam-to-column connections reinforced by welding in steel structures.

Whirl Fire와 Pool Fire의 화염 거동에 관한 연구

오규형,강연옥,이성은 한국안전학회 2004 한국안전학회지 Vol.19 No.3

4-panel of Im height and 45cm width were fixed on the 40cmx40cm bottom plate and the opening of the panel comer was 5cm. Diameter of stainless vessel is 10em and its height is 2cm and it located at the center of the bottom plate. 78mL liquid fuel was filled in the vessel and its depth was lcm. Flame temperature was measured with K type thermocouple, and radiation heat of flame was measured with heat flux meter. Flame height and its behavior was visualized with video camera. and mass burning rate was measured by fuel combustion time. According to the development of fire, flame swirling was begin. From the experiment the mass burning rate was larger and the height of flame was higher than the usual pool fire flame. Flame temperature and heat flux also increased far more than the pool fire. Consequently the swirling air flow through the openings between the panel and thermal buoyance contribute to increase of heat release rate, flame length and mass burning rate.

상악 영구 중철지의 외상성 치근파절 : 증례보고 A CASE REPORT

최형준,곽지윤,이종갑,최병재 大韓小兒齒科學會 2003 大韓小兒齒科學會誌 Vol.30 No.3

청소년기에는 두개악안면부위의 외상성 손상이 흔하다. 그러나 상아질, 백아질, 치수를 포함하는 치아파절로 정의되는 치근파절은 비교적 드물다. 치근파절에 대하여 취할 수 있는 적절한 조치는 파절편의 치관부위를 재위치시키고, 2∼3달간 레진강선 고정술을 시행하는 것이다. 치수과사나 치근흡수의 징후가 나타날때까지 근관치료를 보류해야 하는데 대부분의 증례에서 파절편의 치근부위는 그 생활력을 유지하기 때문이다. 본 증례의 10세 4개월된 남아는 상악영구중절치의 치근파절을 주소로 연세대학교 치과병원에 내원하엿으며, 그후 7년동안 동일부위에 2번의 반복적인 외상이 있었으나 파절편 치관부의 재위치, 레진 강선 고정술, 근관치료 시행 후 임상적으로 방사선학적으로 비교적 양호한 치료결과를 얻었기에 보고하는 바이다. Traumatic injuries in the young permanent dentition are common, but root fractures, defined as fractures in volving dentin, cementum and pulp, are relatively uncommon. Appropriate management of root fracture involves repositioning the coronal portion of the tooth fragment and firm immobilization with a splint for 2 to 3 month. Root canal treatment should not be initiated until the sign of necrosis or resorption are apparent because in most cases, the apical fragments maintain their vitality. The following case report describes a patient with root fractures injured three times over the period of 7 years. The results, clinically and radiographically, were acceptable, but term periodic evaluation is required.

PFC를 적용한 AC/DC 승압형 컨버터 설계

백형래,박수강,최연옥,한엄용,이성길 조선대학교 에너지.자원신기술연구소 1999 에너지·자원신기술연구소 논문지 Vol.21 No.1

Single-Phase diode rectifiers are widely used in industry. These rectifier, however, generate many harmonic components in the AC Power line and have the poor input power factor, which may create a power quality problem for other electrical apparatus in the vicinity of the rectifier. Power factor improvement and harmonic reduction in the AC line current have become very significant problems in single-phase diode rectifiers. In this paper, the high power factor boost converter using soft switching is proposed to improve the demerit. The simulation results show the validity of the proposed system.

혈소판 유래 성장인자에 의한 자연 동맥문합에 관한 연구

양연모,홍성표,이두형 경희대학교 1996 慶熙醫學 Vol.12 No.3-4