토양에서 클로르피리포스의 동력학적 거동

유현철,신남철,우성훈,박승조 東亞大學校 附設 環境問題硏究所 2004 硏究報告 Vol.26 No.-

We have investigated physicochemical characteristics and behavior of chlorpyrifos(CPF) in the agricultural soil, soil + dry waste food(DWF) and soil +manure. Experiments were accomplished biological degradation of samples loaded with chlorpyrifos in incubator at 27℃ during 30days in laboratory. In this study, Sample containing soil + manure has shown to be better biological degradation than sample of soil + DWF. The kinetics of chlorpyrifos oxidation catalyzed by agricultural soil, soil + DWF, and soil + manure can be described by Henri-Michaelis-Menten equation, V=(Vmax)/( Km+ C), where Vmax is the maximum velocity and Km the concentration of the substrate(C) corresponding to an initial(V) half of Vmax. The value of Vmax and km is 0.0588㎍CPF/g.soil.hr and 7.3529㎍CPF/g.soil in agricultural soil, 0.0601㎍CPF/g.soil∙hr and 4.4117㎍CPF/g.soil in the soil loaded DWF and 0.0890㎍CPF/g.soil∙hr and 4.2818㎍CPF/g.soil in the soil added manure. Therefore the order of Vmax. were Manure> DWF> Agricultural soil.

播種量 差異에 따른 濟州在來 동부의 生育反應, 收量 및 粗成分 變化

趙南棋,金東賢,宋昌吉,劉哲受,高東煥 濟州大學校 亞熱帶農業硏究所 1999 亞熱帶農業硏究 Vol.16 No.-

ABSTRACT This study was accomplished to look into the response of main growth characters, yield and chemical composition of Cheju local cowpea on Cheju Island based on the difference between seeding rate. The study took place between May and September, 1998. Number, of days to flowering tended to be delayed as the seeding rate increased. Plant length was the longest at 6kg/10a treatment and the next one was at 5kg/10a treatment. The shortest came from 2kg/10a treatment, 275 cm, 271 and 220cm respectively. Number of branchs and stem diameter became weaker as the seeding rate increased. Fresh forage yields per lOa was the heaviest which weighed 6,073kg at 6kg/10a treatment and 5,840kg at 5kg/10a treatment respectively. The rest decreased gradually. Dry matter yields per lOa, crude protein yields per lOa and total digestible nutrient yields per lOa showed a similar tendency to fresh yields per lOa. The percentage of crude protein and crude fat got higher as the seeding rate increased, but the percentage of crude ash and crude fiber rather tended to decrease. Nitrogen free extract and total digestible nutrient percentages were similar to the change of crude protein percentage. SPAD reading values were the highest at 6kg/10a and 5kg/10a treatment.

외상으로 실활된 미성숙 영구치에서의 계속된 치근 형성

강유진,김혜영,김영진,김현정,남순현 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.4

치아에 외상을 받은 경우 치수 생활력의 상실은 흔한 일이다. 치수 생활력을 검사하는 방법으로는 임상적, 방사선학적으로 여러 가지가 있지만, 미성숙 외상치의 경우 일시적 현상과 가성 반응이 나타날 수 있으므로 치수 괴사에 대한 정확한 진단은 매우 어렵다. 생활력을 상실한 치아는 염증성 치근흡수, 치근단 낭종 등의 발생을 방지하기 위하여 치수 치료를 시행한다. 그러나 미성숙 영구치의 경우, 치수 치료를 시행 후 치근 성장이 정지될 수 있어 결과적으로 얇고 짧은 치근이 형성되어 장기적인 예후는 좋지 않다. 본 임상 증례에서는 외상으로 인하여 실활된 초기 영구치에서 치근단부의 최소한의 침습적 치근단 형성술로 계속된 치근 형성을 보여 이에 보고하는 바이다. In case of luxation injuries, loss of tooth vitality is common. And in case of trauma in the immature permanent teeth, precise diagnosis of pulp necrosis is very difficult. That is because limitation in distinguishing between normal dental papilla in immature permanent teeth, transient apical breakdown(TAB), which is part of normal healing process, and apical radiolucency in pulp necrosis. Especially in non-vital immature permanent tooth, the treatment is complex and requires long time. This clinical case report shows that severely infected immature teeth with periradicular periodontitis can undergo healing and apexogenesis or maturogenesis with no definative treatment or after conservative treatment. In the cases reported, we emphasize the considerable power of regeneration of the tooth, probably due to its large number of undifferentiated mesenchymal cells in the dental papilla, pulp tissue, periodontal ligament tissues. Thus, when endodontic treatment in immature permanent teeth, over instrumentation is not recommend for preserve the apical vital stem cells.

랫드에서 단일시험법을 이용한 반하 물추출물의 생식독성시험

이기남,유욱준,백인정,연정민,남상섭,이범준,남상윤,김윤배,강현규,안병우,김대중,황방연,노재섭,홍정섭,성하정,김순선,조대현,윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

본문참조

외상으로 유착된 영구 전치에서의 치관 절제술

강유진,김영진,김현정,남순현 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.2

외상으로 치주 인대의 손상이 심한 치아에서는 치아-치조골 유착이 흔히 나타난다. 성장기 아동에서의 치아 유착의 경우 치조골의 성장 중단을 초래하여 저위 교합이 나타나며 이로 인하여 청소년기에 심미적 불만족이 생길 뿐 아니라 인접치의 경사가 발생한다. 이는 교정 또는 보철 치료를 어렵게 만들 수 있으므로 유착된 치아는 비교적 조기에 치료한다. 하지만 저위 교합된 치아를 발치할 경우 치조제의 상당한 소실을 유발할 수 있으며 특히 상악 전치부의 앓은 순측골의 위축을 가져와 심미적인 보철물을 형성하기가 어려워진다. 이러한 치조골의 소설을 방지하기 위하여 치아의 치관부는 제거하고 유착된 치근은 골에 의해 대체되도록 치조골에 남겨두는 치관절제술을 행하는 것이 바람직할 것으로 생각된다. 본 증례에서는 만 9세와 10세에 상악 전치부 치아의 외상을 받은 후 치근 유착이 발생하여 저위 교합을 보이는 성장기 남아에서 추후 예상되는 보철적, 교정적 합병증의 가능성을 감소시키기 위하여 치관절제술을 시행하였다. 저위교합이 2-3 mm정도 진행되었을 때 술식을 행하였으며, 환아의 성장을 고려하여 한국인의 최대 성장 완료 시기인 16세 이전에 술식을 행하였다. 계속적인 임상적, 방사선학적 검사 결과 수평적 치조골의 소실이 방지될 뿐 아니라, 치조골의 수직적 증가를 보이며, 남겨진 치근은 서서히 흡수되는 양상을 보였기에 이를 보고하고자 한다. Trauma commonlyleads to ankylosis for the tooth whose periodontal ligaments have been injured. In growing patients, an ankylosed tooth can disrupt alveolar development, resulting in infraocclusion of the affected tooth. Consequently, this causes aesthetic problems during adolescence, interferes with prosthetic treatment due to th.e inclination of adjacent teeth, and complicates orthodontic dental movement. When the infraoccluded ankylosed tooth is extracted, a considerable amount of alveolar bone is lost, especially in the maxillary anterior region. Moreover, depression of the thin buccal alveolar bone compromises aesthetic restoration. In order to prevent alveolar bone loss, the ankylosed tooth should be treated by decoronation. In the present study, the traumatically injured maxillary incisors of 9-year-old and 10-year-old patients that present infraocclusion accompanied by ankylosis were treated with decoronation procedures. Decoronation procedures were performed when the affected teeth were 2-3 mm below relatively to the adjacent teeth. Moreover, the patients were treated before they get to 16 years old considering the maximum growth peak of the korean adolescents. As the results, there are favorable clinical results associated not only with preservation of horizontal alveolar volume but also with increase in vertical alveolar height after decoronation in growing individuals.

녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

변이주인 Trichoderma reesei YC-3156이 생산하는 Cellulase에 관한 연구

오두환,안순철,유주현,허남윤 연세대학교 산업기술연구소 1987 논문집 Vol.19 No.2

After the treatment 15 strains of Trichoderma sp. and 4 isolates with N-methyl-N´-nitro-nitrosoguanidine, T. reesei YC-3156 among the mutants showed the highest cellulase activity. The Avicelase of T. reesei YC-3156 was purified by 70% ammonium sulfate fractionation, dialysis, DEAE-Sepharose column chromatography and Sephadex G-100 Gel-filtration. With the result of the procedures, its purification degree was 13 folds, yield 9.3% and specific activity 0.9 unit/mg. The homogeneity and the molecular weights of the purified Avicelase were determined by SDS-polyacrylamide gel electrophoresis, it was electrophoretically homogeneous and showed the molecular weights of 46,000-48,000. The purified Avicelase was stable between pH 4.0 and pH 5.0 and it was inactivated rapidly above 60℃.

흡연에 의한 급성 호산구성 폐렴 2예

박종빈,김학렬,주현준,유태양,신성남,신정현,송정섭,황기은,김소영,양세훈,정은택 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

최근 흡연을 시작한 젊은 성언에서 급성 호산구성 폐렴의 발생에 대한 증례가 보고되고 있다. 급성 호산구성 폐렴은 대개 발열을 동반하기 때문에 초기에는 감염성 폐렴으로 잘못 진단, 치료되는 경우가 많다. 아직 정확한 병태 생리 및 조직학적 소견은 밝혀져 있지 않지만 병력 청취 및 임상 양상, 방사선 소견의 관찰을 통해 급성 호산구성 폐렴을 의심하고, 진단을 위해 기관지 폐포 세척액 검사를 시행한다면 좀 더 쉽게 진단 내릴 수 있을 것이다. 본 저자들은 최근 처음 시작한 흡연에 의해 발생한 급성 호산구성 폐렴을 진단하고, 스테로이드를 투여하여 성공적으로 치료한 환자 2예를 경험하였기에 이를 문헌 고찰과 함께 보고하는 바이다. Acute eosinophilic pneumonia(AEP) is characterized by acute febrile respiratory illness associated with diffuse pulmonary infiltration and pulmonary eosinophilia. The specific etiology for acute eosinophilic pneumonia is elusive. By some investigators, cigarette smoking is suggested as a causative substance which can cause AEP. In recent, the authors experienced two cases of AEP following cigarette smoking. Both cases had characteristic features including age around 20 years, new onset smoking before occurance of AEP, diffuse infiltration on chest radiography, pulmonary eosinophilia based on bronchoalveolar lavage and acute improvement after steroid therapy. These clinical features are resemble with previous smoking induced AEP case reports. Base on these clinical features, cigarette smoking associated AEP could be diagnosed more easily.

Effects of Cortical Activation upon Mechanical Force-Mediated Changes in the OPG and RANKL Levels in Gingival Crevicular Fluid

Nam-Hyun Yu,So-Yeong Kwak,So-Yeon Hong,Jong-Ghee Kim,Young-Mi Jeon,Jeong-Chae Lee KOREAN ACADAMY OF ORAL BIOLOGY 2009 International Journal of Oral Biology Vol.34 No.4

This study investigated whether orthodontic force influences the production of osteoprotegerin (OPG) and receptor activator of nuclear factor-kappa B ligand (RANKL) in vivo, both of which are affected by cortical activation. Mechanical force was applied to the maxillary premolars of orthodontic patients by fitting the transpalatal arch prior to cortical activation of the gingival tissue. Gingival crevicular fluid (GCF) samples were then collected from each patient using paper strips before and after 1, 3, 7 or 14 days of treatment. The OPG and RANKL levels in the GCF were determined by enzyme-linked immunosorbent assays. The levels of OPG were significantly increased after 1 day of fitting the appliance and decreased to basal levels at 3 days after fitting. In contrast, the RANKL levels were dramatically decreased at 1 day after fitting, but recovered to those of the untreated control at 3 days after the force application. The force-mediated changes in the OPG and RANKL levels of the GCF were unaffected by cortical activation during these experimental periods. Collectively, these results suggest that an acute and severe change between the OPG and RANKL levels plays an important role in stimulating the cellular responses required for alveolar bone remodeling by orthodontic treatment.

Apoptosis induced by caffeic acid phenethyl ester in human oral cancer cell lines: Involvement of Puma and Bax activation

Yu, Hyun-Ju,Shin, Ji-Ae,Yang, In-Hyoung,Won, Dong-Hoon,Ahn, Chi Hyun,Kwon, Hye-Jeong,Lee, Jeong-Sang,Cho, Nam-Pyo,Kim, Eun-Cheol,Yoon, Hye-Jung,Lee, Jae Il,Hong, Seong-Doo,Cho, Sung-Dae Elsevier 2017 Archives of oral biology Vol.84 No.-

<P><B>Abstract</B></P> <P><B>Objective</B></P> <P>Caffeic acid phenethyl ester (CAPE), a natural honeybee product exhibits a spectrum of biological activities including antimicrobial, anti-inflammatory, antioxidant and antitumor actions. The purpose of this research was to investigate the anticancer potential of CAPE and its molecular mechanism in human oral cancer cell lines (YD15, HSC-4 and HN22 cells).</P> <P><B>Design</B></P> <P>To determine the apoptotic activity of CAPE and identify its molecular targets, trypan blue exclusion assay, soft agar assay, Western blot analysis, DAPI staining, and live/dead assay were performed.</P> <P><B>Results</B></P> <P>CAPE significantly suppressed transformation of neoplastic cells induced by epidermal growth factor (EGF) and 12-O-tetradecanoylphorbol 13-acetate (TPA) without inhibiting growth. CAPE treatment inhibited cell growth, increased the cleavages of caspase-3 and poly (ADP-ribose) polymerase (PARP), and augmented the number of fragmented nuclei in human oral cancer cell lines. CAPE activated Bax protein causing it to undergo a conformational change, translocate to the mitochondrial outer membrane, and oligomere. CAPE also significantly increased Puma expression and interestingly Puma and Bax were co-localized.</P> <P><B>Conclusion</B></P> <P>Overall, these results suggest that CAPE is a potent apoptosis-inducing agent in human oral cancer cell lines. Its action is accompanied by up-regulation of Bax and Puma proteins.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CAPE suppresses neoplastic cell transformation induced by EGF and TPA. </LI> <LI> CAPE has apoptotic effect via Puma and Bax activation in oral cancer. </LI> <LI> Possibility of CAPE as potent apoptosis-inducing agent in oral cancer is proposed. </LI> </UL> </P>