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      • The insect community structure with indicator species along vegetation composition in Is. Nam-hae, South Korea

        Jong Woo NAM,Shin Young PARK,Ik Jae CHOI,Young Min SHIN,Il-Kwon KIM 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.04

        An insect faunal survey was carried out to investigate insect community structure along the vegetation community to monitor insect species in forest ecosystem. We performed day and night collections from June to August along three vegetation communities of Is. Nam-hae in 2014: the first stand with Pinus thunbergii, the second P. thunbergii with Quercus serrata and the third P. thunbergii with various Quercus species. In total 2,259 individuals of 532 species, 99 families, 13 orders are identified. Cluster Analyses (CA) showed that all three vegetation communities were relatively similar between vegetation community types. According to indicator species analysis (ISA) result, nine significant indicator species were identified (p < 0.05); five species were found to be affected by the vegetation cluster and four species the month cluster.

      • KCI등재후보

        한국인 인슐린 비의존형 당뇨병 및 이차성 당뇨병 환자에서 글루코키나제 유전자 변이

        남재현(Jae Hyun Nam),이현철(Hyun Chul Lee),김연의(Youn Euy Kim),권석호(Suk Ho Kwon),윤용석(Yong Suk Yoon),박석원(Suk Won Park),원영준(Young Jun Won),차봉수(Bong Su Cha),송영득(Young Duk Song),이은직(Eun Jig Lee),임승길(Sung Kil Lim) 대한내과학회 1998 대한내과학회지 Vol.54 No.6

        Objectives: Mutations in the glucokinase (GCK) gene are considered a possible cause of maturity-onset diabetes of the young. The purpose of this study was to evaluate the contribution of this gene to the development of non insulin dependent diabetes mellitus (NIDDM), gestational diabetes mellitus (GDM) and post-renal transplantation diabetes mellitus (PTDM). Method: Identification of GCK mutation was attempted on 39 NIDDM patients, 2 GDM patients and 58 selected renal allograft recipients with PTDM and 45 normal controls. The exons in the GCK gene were examined by polymerase chain reaction (PCR), followed by analysis of single-stranded DNA conformational polymorphism (SSCP). The abnormal bands were also confirmed by DNA sequencing analysis. The exons of affected family members were also investigated for mutations of the GCK gene. Results: Two of the 58 PTDM patients (3.4%) were found to have CKK mutations. One had the mutation on exon 5 and the other on intron 7. One control subject had the mutation on intmn 9. The mutation of exon 5 was identified as a substitution of CCT (proline) for CTT (leucine) at codon 164, which has not ever reported before. The family members of the PFDM patient with mutation of exon 5 were analyzed by PCR followed by SSCP, and two of them revealed the same mutation. The abnormal band on the SSCP analysis of exon 7 was identified as the insertion of base C/T at the 39th nucleotide in intron 7. Two family members of this patients also had same band on SSCP. The one mutation of 45 normal controls was CT located at the 8th nucleotide in intron 9, which was a common polymorphism. Conclusion: We found GCK mutations in subjects with PTDM and we speculate that these mutations may be one of the contributing cause of PTDM.

      • SCOPUSKCI등재

        Cyclodextrin Glucanotransferase와 Cyclodextrinase를 생산하는 Bacillus 속 세균의 분리와 그 효소들의 특성

        권현주,남수완,김광현,곽영규,김병우 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

        토양으로부터 CGTase와 CDase를 함께 분비·생산하는 내열성 세균을 분리하였으며, 동정결과 Bacillus stearothermophilus로 판명되어 KJ16으로 명명하였다. Bacillus stearothermophilus KJ16 균주는 회분배양할 경우 2단계 성장을 하며 일차증식기에 CGTase를 생산하고 이차증식기에 CDase를 생산하는 양상을 보였다. 배치 pH를 7.0으로 조절하면서 배양할 경우, CGTase는 균의 증식에 따라 증가하고 CDase는 CGTase보다 뒤에 생산되었다. 그 생산양은 pH 비조절 배양에 비해 CGTase는 최대 12배(1300 mU/ml)까지, CDase는 2배(225 mU/ml)정도 증가되었다. 분리균주가 생산하는 CGTase는 분자량이 65,000 dalton으로 pH 6.0, 60℃에서 최적활성을 보이며 HPLC 분석을 통해 α-, β-, γ-CD를 42: 46: 12의 비율로 생산하는 α/β 혼합형 CGTase였다. 또한 CDase는 분자량이 68,000 dalton으로 pH 6.0, 55℃에서 최적활성을 보이며 최적기질은 γ-CD였다. A bacterium named as Bacillus stearothermophilus KJ16. The growth of the isolated strain occurred in two steps, and syntheses of CGTase and CDase were dependent on the growth cycle of the cell. CGTase was constitutively synthesized during the 1st growing phase, while CDase was synthesized inducibly during the 2nd growing phase. When the medium pH was controlled at 7.0, the maximun enzyme activities of CGTase and CDase were increased by 12-fold(1300 mU/ml) and 2-fold (225 mU/ml), respectively, compared with the pH-uncontrolled batch culture. The CGTase of the isolate converted soluble starch to CDs with the ratio of α-CD: β-CD: γ-CD=42:46:12 at 55℃. The optimal pH and temperature of CGTase were 6.0 and 60℃, respectively and the optimal pH and temperature of CDase were 6.0 and 55℃. The molecular weights of the purified CGTase and CDase were estimated to be 65,000 and 68,000 dalton, respectively. Among serveral substrates, γ-CD was most rapidly hydrolyzed by the purified CDase.

      • KCI등재

        해마 theta리듬과 pyramidal neuron의 세포내 특성과의 상관관계

        권오흥,조진화,남순현,김현정,최병주,김영진,이만기 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.4

        Eledctrophysiological phenomena of pyramidal cells in the CAI area of the dorsal hippocampus were recorded from and filled with neurobiotin in anesthetized rats. The electropharmacological properties of membrane as well as the cellular-synaptic generation of rhythmic slow activity (theta)were examined. The intracellular response characteristics of these pyramidal cells were distinctly different from responses of inteneurons. Pyramidal cells had a high resting membrane potential, a low input resistance, and a large amplitude action potential. A afterhyperpolarization was followed a single action potential. Most of pyramidal cells did not display a spontaneous firing. Pyramidal cells displayed weak inward rectification and anodal break excitation. The slope of the frequency-current relation was 53.4 Hz/nA for the first interspike interval and 15.9 Hz/nA for the last intervals, suggesting the presence of spike frequency adaptation. Neurobiotin-filled neurons showed pyramidal morphology. Cells were generally bipolar dendritc processes ramifying in stratum lacunosum-moleculare, radiatum, and oriens. Commissural stimulation discharged pyramidal cells, followed by excitatory and inhibitory postsynaptic potentials (EPSPs and IPSPs). The frequency of theat-related membrane potential oscillation was voltage-independent in pyramidal neurons. At strong depolarization levels (less than 30 mV) pyramidal cells emitted sodium spike oscillation, phase-locked to theta. The observations provide direct evidence that theta-related rhythmic hyperpolarization of principal cells is brought by the rhythmically discharging interneurons. Furthermore, the findings in which interneurons were also paced by rhythmic inhibitory postsynaptic potentials during theta suggest that they were periodically hyperpolarized by their GABAergic septal afferents.

      • 5-Phenyl-4,5-dihydro-1H-benzazepin-2 one의 합성

        권순경,박영남 德成女子大學校 2003 德成女大論文集 Vol.32 No.-

        The first of benzodiazepines was chlordiazepoxide, which was marketed as tranquilizer under the trade name Librium. Now benzodiazepine derivatives are prescribed for insomnia and muscle relaxants as well as to treat anxiety. As benzodiazepines used widely, drug abuse becomes a serious social problem because of psychological and physical dependence and over deaths. It is necessary to develop the in vitro diagnostic reagents for the detection of small amount of abused drugs in urines. Because accuracy and convenience for point of care testing systems are indispensable, immunological determinations are fit for our requirements, which react with specific drug antigens and are able to detect small amount of drugs in urine. A benzazepinone derivative(4), which will be used as a component of the reagents for detecting benzodiazepine drugs or their metabolite in urine, was synthesized from 5-phenyltetrahydrofuran-2-one(1) as a key intermediate.

      • 한국의 재활의료 전달체계

        권혁철,정영일,남은우 高神大學校 保健科學硏究所 1993 보건과학연구소보 Vol.3 No.-

        This study was carried out for reorientation of Rehabilitation Service Delivery System (RSDS) through reviewing reference and observing field practice. The most important results are as follows: 1. The current rehabilitation facility for handicapped persons can be classified into four categories : (a) those for physically disabled persons, (b) those for mentally disturbed persons, (c) those for visually disturbed persons, and (d) those for acoustic and speech disturbed persons. The nationwide number of rehabilitation facilities is 101 of those, 43.5% is for physically disabled persons and 32.9% is for mentally disturbed persons. 2. A small number of circulating rehabilitation centers for physically disability are being run by comprehensive rehabilitation centers since 1992. 3. The hospitals are self-supporting only 20% of their operational costs, and the rest of the operational costs comes from federal tax(40%) and local tax(40%). 4. The number of handicapped persons in Taegu and Kyungpook province is estimated to be about 50,000, but only 20 percent out of them ws registered. The rehabilitation facilities located in this area receives 69% of their operational costs from central government. This figure is higher than the national average of 64.6%. 5. Although RSDS is different from other medical delivery system, nonetheless, it should be developed as a sub-system of national health care delivery system. Its development should also be in accordance with the need of local community. Therefore we suggest the development policy for the development of RMDS must consider the following points.; First, all rehabilitation-related medical systems should be integrated unde ra single comprehensive system so that handicapped persons may easily obtain better service available. Second, rehabilitation programs based on local community should be rejuvenated. In addition to in-patient service, development of intermediate facilities and programs connecting home and society should be encouraged. Third, rehabilitation medical services should be more specialized. Also, more emphasis should be given to team approach to rehabilitation medical services. Fourth, a comprehensive rehabilitation information system should be developed so that handicapped persons may have easy access to public welfare facilities, and the central government should formulate a comprehensive long-term plan for the rehabilitation system.

      • ω-(2-Oxo-5-Phenyl-2,3-dihydro-1H-benzazepinyl)alkanoic acids의 합성

        권순경,박영남 德成女子大學校 藥學硏究所 2003 藥學論文誌 Vol.14 No.1

        Benzodiazepines are used widely for medical purposes and drug abuse becomes a serious social problem because of psychological and physical dependence and overdose deaths. For accuracy and convenience to detect small amount of drugs in urine, it is necessary to develop a rapid in vitro detecting method. For this requirement the immunological determination reagent which reacts a specific drug antigens is one of the best method. A benzazepinylalkanoic acid which can be used as a component of the reagents for detecting benzodiazepine drugs or their metabolite in urine, was synthesized from 5-phenyl-4, 5-dihydro-1H-benzazepin-2-one(1).

      • Effect of Meteorological Element on Growth and Yield of Sesame

        Kwon,Byung-Sun,Shin,Jeong-Sik,Shin,Jong-Sup,Choi,Seong-Kyu,Seo,Young-Nam 한국자원식물학회 2002 Plant Resources Vol.5 No.3

        This study was conducted to investigate the relationships between yearly variations of climatic elements and yearly variations of productivity in sesame. In addition, correlation coefficients among yield and yield components were estimated. The data of yield and yield components were investigated for 10 years from 1992 to 2001. The meteorological data gathered at the Yeosu Weather Station for the same period were used to find out the relationships between climatic elements and productivity. Yearly variation of the amount of precipitation in July and September were large with coefficients of variation(c.v.) of 64.59, 92.47%, respectively, but the variation of the average temperature in June and August were relative small. Yield and plant height greatly with c. v. of 26.24, 23.41 %, respectively, 1, 000 grain weights show more or less c.v. of 3.83% and length capsule setting show still less variation. Correlation coefficients between maximun temperature in period of cultivation(from June to September) and yield are positively significant at the level of 5.1 %, respectively. Correlation coefficients amount the plant height, length capsule setting, number of capsules per plant, weight of 1, 000 grains and seed yield were positively significant at the level of 1 %, respectively. Simple linear regression equations by the least square method are estimated for number of capsules per plant(Y₁) and the maximun temperature in August(X) as Y₁=10.1255+0.1725X, and for yield(Y₂) and the maximun temperature in August(X) as Y₂=21.6151 + 1.3724X.

      • KCI등재후보

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