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      • 알레르기 환자에서 MAST-CLA 검사의 의의

        남해선,황규윤,,백병준,정윤영,오천환,조성란,박준수 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

        To evaluate usefulness of application of MAST-CLA to indetify allergens, authors conducted MAST-CLA allergen-specific IgE assay and collected allergy related symptoms in 118 allergic patients diagnosed at Soonchunhyang University Chunan Hospital during October 1998 through January 2000. The results were as followings; 1. The mean (SD) age was 23.1 (±12.17) years old and teenagers accounted for 33.1% of patients. 2. The frequent allergic symptoms were sneezing (59.3%), rhinorrhea (53.4%), nasal stiffness (44.1%), itching sensation (24.6%), cough or sputum (16.1%), and eye symptom (8.5%) in order. 3. Among these subjects, 62.7% of patients revealed at least more than one specific allergen and 70.3% showed elevated total IgE levels by MAST-CLA. The three most frequent specific allergens were Dermatophagoides farinae (47.5%), Dermatophagoides pteronyssinus (45.8%), and house dust (30.5%). 4. While age was not significantly related to positive of both specific allergens and total IgE (P>0.05), sneezing and nasal stiffness were significantly associated with positive of specific allergen and total IgE, respectively (both P<0.05). 5. Total IgE increased with the number of specific allergens, but both specific allergen and total IgE were not significantly related to increases of allergic symptoms (P>0.05). In conclusion, our data suggest that MAST-CLA allergen-specific IgE assay is useful measure for identification of causative allergens in allergic patients, however, it is recommended that more specific allergic antigens should be determined and replaced or added to Korean inhalent panel of MAST-CLA.

      • KCI등재

        저선량 γ선 조사가 참박의 초기 생육과 효소 활성 및 광합성 능에 미치는 영향

        이혜연,김재성,백명화,이영근,임돈순 한국환경생물학회 2002 환경생물 : 환경생물학회지 Vol.20 No.3

        저선량 γ선 조사가 참박의 초기생육과 생리활성에 미치는 효과를 알아보기 위하여 참박 종자에 γ선을 O~2OGy 수준으로 조사하여 생육을 관찰하였다. 저선량 γ선에 의한 참박의 초기생육은 대조구에 비해 4~16Gy에서 다소 증가하였고, 생육 조사시 측정한 자엽의 경우 catalase와 peroxidase활성이 대조구에 비해 8Gy조사구에서 가장 높았으며 본엽은 peroxidase 활성이 4Gy 조사구에서 확연히 증가하였다. 저선량 γ선을 조사한 박 식물체의 광 스트레스에 대한 반응효과는 광계Ⅱ의 광화학적 효율이 대조구와 8Gy의 경우 50%정도 감소되었으나 4Gy에서는 40%정도의 감소를 보였다. 반면 Fo는 광저해가 진행됨에 따라 약간의 증가를 보였으나 대조구나 저선량 조사구사이의 차이는 거의 없는 것으로 나타났다. 광계Ⅱ의 광양자 수율, φ_PSⅡ과 광계Ⅱ 반응중심의 흥분 포획능, 1/Fo-l/Fm 또한 광저해가 진행되는 동안 감소하여 φ_PSⅡ는 대조구와 8 Gy의 경우 20%정도 감소를 보인 반면 4Gy조사구는 15%의 감소를 보였고, 1/Fo-lF/m은 대조구와 8Gy는 55%,4 Gy는 45%의 감소를 나타냈다. 비광화학적 소멸인 NPQ는 대조구와 저선량 조사구 모두 70% 정도 감소되는 경향을 보였다. 이러한 결과를 볼 때 종자의 종피를 투과한 저선량의 γ선이 참박의 생육을 촉진시키고 4Gy에서 광 스트레스에 대한 저해가 감소되었다. Gourd seeds were irradiated with the doses of 0∼20 Gy to investigate the effect of the low dose ??-radiation on the early growth and physiological activity. The stimulating effects of the low dose ??-radiation on the early growth were not noticeably high, but were increased generally at 4∼16 Gy irradiation group. The catalase and peroxidase activity of cotyledon from seeds irradiated with ??-radiation were increased at 8 Gy irradiation group. The peroxidase activity of leaf was noticeably high at 4 Gy irradiation group. The photochemical yield of PSⅡ, estimated as Fv/Fm, decreased with increasing illumination time by 50% after 4hrs in the control and 8 Gy irradiation group, while Fo slightly increased. However, Fv/Fm in the 4 Gy irradiation group decreased by 40% of inhibition, indicating that photoinhibition decreased by the low dose ??-radiation. Changes in the effective quantum yield of PSⅡ, Φ_PSⅡ and 1/Fo-1/Fm, a measure of the rate constant of excitation trapping by the PSⅢ reaction center, showed similar pattern to Fv/Fm. NPQ decreased by 70% after photoinhibitory treatment with showing similar pattern between the control and the irradiation group. These results showed the positive effect of low dose ??-radiation on the seedling growth and the reduction of photoinhibition in the 4 Gy irradiation group.

      • SCOPUSKCI등재

        견갑부 유리피판술 : ITS ANATOMY & CLINICAL APPLICATIONS 해부학적 구조 및 임상적 고찰

        김홍래,백세민,이혜영 大韓成形外科學會 1983 Archives of Plastic Surgery Vol.10 No.2

        Since the first successful microvascular free flap was performed by Daniel & Taylor in 1973, it has been popularized in the plastic and reconstructive surgery. The free skin flap has proved its great versatility as compared with conventional pedicle flap methods. It can be applied to extensive skin defects and one-stage pedicle flap procedure, and hospital stay is shorter. Early ambulation and rehabilitaion are possible. In 1980, Frenklin reported on the use of deltoid free flap, which was based on posterior circumflex humeral vessel. The flap has relatively thin subcutaneous tissue, but has thicker dermal layer, so provides stability to the sole and heel of foot. Anatomically the plane of dissection is easily identified. The donor scar is minimal, and functionally and cosmetically acceptable. Authors have good result using deltoid free flap for reconstruction of shallow tissue defects.

      • 잔류자계를 이용한 속도검출용 자계센서

        신사현,장용해,백형래,조금배 조선대학교 전자정보통신연구소 2001 電子情報通信硏究所論文誌 Vol.4 No.2

        In this paper, describe the magnetic sensor detecting the speed of wire or thin pipe, which move irregularly, using Hysteresis phenomenon. After magnetic field is placed to a metallic object, this magnetic sensor converts residual magnetism into velocity, This magnetic sensor is composed of transmission part that causes magnetism, reception part that detects magnetism, and control part that convert magnetic field intensity into velocity.

      • 순무(Brassica campestris ssp. rapa) 뿌리로부터 지질화합물의 분리 및 동정

        방면호,오영준,유종수,한민우,정해곤,정태숙,최명숙,이경태,김세영,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

        순무 뿌리로부터 활성 물질을 분리 동정 하기 위하여 80% MeOH 수용액으로 추출하고 이를 여과, 감압 농축하여 MeOH추출물을 얻었다. 이를 EtOAc분획, n-BuOH분획, H_(2)O분획으로 나누었으며, EtOAc분획에 대해 silica gel column chromatography를 실시하여 3종의 지질화합물을 분리 정제하였다. ^(1)H-NMR, ^(13)C-NMR, DEPT spectrum 및 Mass spectrum등을 통하여 palmitic acid methyl ester(compound 1), linolenic acid methyl ester(compound 2), linoleic acid methyl ester(compound 3)으로 구조를 결정하였다. In order to isolate and identify the active compound from the Brassica campestris ssp rapa, the roots and the root skin were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H_(2)O. From the EtOAc fraction, three compounds were isolated through the repeated silica gel column chromatographies. From the result of spectral data including NMR and MS, the chemical structures of the compound were determined as palmitic acid methyl ester (compound 1), linolenic acid methyl ester (compound 2), linoleic acid methyl ester (compound 3).

      • 강화 특화작목 지표물질의 함량 분석

        유종수,방면호,정해곤,정태숙,최명숙,이경태,김세영,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

        강화 특화작목의 건강기능성 식품 제품화의 가능성을 타진하고 재배작물의 표준화 연구를 위해 사자발쑥, 싸주아리쑥 그리고 순무에 대해 총 폴리페놀과 총 플라보노이드 함량을 측정하였다. 총 폴리페놀 함량은 사자발쑥이 9.5mg/g, 싸주아리쑥이 7.4mg/g, 순무가 1.8mg/g으로 총 플라보노이드 함량은 사자발쑥이 4.3mg/g, 싸주아리쑥이 3.6mg/g, 순무가 0.4mg/g으로 확인 되었다. 또한 강화 약쑥의 유효지표성분인 eupatilin과 jaceosidin이 사자발쑥에는 각각 208mg/100g, 118mg/100g, 싸주아리쑥에는 199mg/100g, 111mg/100g 함유된 것을 HPLC를 통해 확인하였다. This study was conducted to investigate the contents of total polyphenol and total flavonoid of three Ganghwa indigenous crops, two varieties of Artemisia princeps Pampanini (Sajabalssuk, Sajuarissuk) and Brassica campestris ssp rapa. Total polyphenol and total flavonoid contents of Sajabalssuk were 9.5 and 4.3 mg/g, those of Sajuarissuk were 7.4 and 3.6 mg/g, those of B. campestris ssp rapa were 1.8 and 0.4 mg/g, respectively. The contents of eupatilin and jaceosidin in A. princeps Pampanini which were calculated by HPLC, were determined 208 mg/100g, 118 mg/100g in Sajabalssuk and 199 mg/100g, 111 mg/100g in Sajuarissuk, respectively.

      • 조혈모세포이식 후 발생한 주폐포자층 폐렴에 대한 고찰

        주지현,최정현,이동건,백지연,고윤호,이혜정,김세희,신호진,박윤희,박지영,김유진,신완식,김춘추 대한감염학회 2001 감염 Vol.33 No.4

        Background : Pneumocytitis cainii pneumonia (PCP) can occur in immunocompromised hosts especially such as AIDS or cancer patients. Although recent research had focused on PCP in AIDS patients, few studies have described the clinical presentations of PCP in recipients of stem cell transplantation (SCT). We evaluated the clinical manifestations of PCP in SCT patients admitted at St. Mary's hospital, Seoul, Korea. Methods : The medical records of 17 PCP patients undergoing SCT between Feb. 1998 and Feb. 2000 were reviewed. The diagnosis of PCP was confirmed through the demonstration of Pneumocytitis cainii via either cytology of brochoalveolar lavage (BAL) or histological technique of lung biopsy. CMV disease and CMV infection were confirmed by BAL culture and antigenemia respectively . Results : Seventeen patients were all recipients of allogeneic SCT and 7 of 17 patients were performed non-sibling SCT. Patients presented with symptoms including brief period (4 ∼23 days) of fever (76%), dyspnea (70%), cough (64%), and signs such as rare(58.8%), Sixteen patients (94%) had been receiving immunosuppressive agent such as cyclosporine A (64%) or Fk506 (35%) without PCP prophylaxis. Eleven patients (64%) were treated with corticosteroid with mean dose of 16 mg/day prednisolone and mean duration of 4.6 months after post-SCT period. Twelve patients were co-infected with CMV. Another co-infected miCroorganisms were Pseudomonas aeruginosa, Mycobacterium tuberculosis, herpes simplex virus, parainfluenza virus, Average duration of treatment with trimethoprim-sulfamethoxazole (TMP/SMX) was 21 ±9 days. Four patients died, and three of them were related with PCP. Conclusion : PCP developed frequently in patients who were taking immunosuppressive drug due to graft versus host disease or were not taking TMP/SMX prophylaxis. High risk patients showing fever, cough, or dyspnea should be considered to take early bronchoscopic intervention for detection of PCP. When treat for PCP, it also be considered to the possibility of coinfection such as CMV. (Korean J Infect Dis 33:273∼279, 2001)

      • 증예(症例) : 불량예후에 대한 여러 고 위험인자를 가지로 있는 양측성 기종성 신우신염 치료 1에

        백영하 ( Young Ha Baek ),권대헌 ( Dae Hun Kwon ),안대선 ( Dae Seon Ahn ),유혜민 ( Hae Min Yu ),윤해은 ( Hae Eun Yun ),정범수 ( Bum Su Joung ),홍영민 ( Young Min Hong ),박경택 ( Kyung Taek Park ),정종환 ( Jong Hwan Chong ),한영민 ( 전북대학교 의과학연구소 2010 全北醫大論文集 Vol.34 No.1

        Patient outcomes with emphysematous pyelonephritis vary with the classification type and other factors. This is a case of bilateral emphysematous pyelonephritis with several risk factors for adverse outcomes. A 71-year-old diabetic woman was referred to us in stuporous state with fever. The patient`s temperature was 38.7 ℃ and blood pressure was 90/60 mmHg. The abdomen was distended with severe flank knocking tenderness. The laboratory findings showed white blood cell count of 26,400/mm3, hemoglobin level of 10.5 g/dl, platelet count of 46,000/mm3, glucose level of 422 mg/dl, BUN of 163 mg/dl, and creatinine level of 5.5 mg/dl. Urinalysis showed 1+ proteinuria, 1+ glycosuria, 25-30 WBCs/HPF, and 25-30 RBCs/HPF. Unenhanced computed tomography demonstrated multiple gas pockets in renal parenchyma, pelvis, ureter, and bladder. Patient was immediately treated with percutaneous catheter for drainage of gas and purulent material, and parenteral antibiotics. E.coli was isolated from both blood and urine cultures. This patient had several concomitant risk factors for adverse outcomes, such as thrombocytopenia, acute renal failure, impaired consciousness, shock and involvement of both kidneys, which are usually associated with significantly increased risk of mortality. However, the patient was successfully treated without undergoing nephrectomy.

      • KCI등재

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