Choline dehydrogenase interacts with SQSTM1/p62 to recruit LC3 and stimulate mitophagy.

Park, Sungwoo,Choi, Seon-Guk,Yoo, Seung-Min,Son, Jin H,Jung, Yong-Keun Landes Bioscience 2014 AUTOPHAGY Vol.10 No.11

<P>CHDH (choline dehydrogenase) is an enzyme catalyzing the dehydrogenation of choline to betaine aldehyde in mitochondria. Apart from this well-known activity, we report here a pivotal role of CHDH in mitophagy. Knockdown of CHDH expression impairs CCCP-induced mitophagy and PARK2/parkin-mediated clearance of mitochondria in mammalian cells, including HeLa cells and SN4741 dopaminergic neuronal cells. Conversely, overexpression of CHDH accelerates PARK2-mediated mitophagy. CHDH is found on both the outer and inner membranes of mitochondria in resting cells. Interestingly, upon induction of mitophagy, CHDH accumulates on the outer membrane in a mitochondrial potential-dependent manner. We found that CHDH is not a substrate of PARK2 but interacts with SQSTM1 independently of PARK2 to recruit SQSTM1 into depolarized mitochondria. The FB1 domain of CHDH is exposed to the cytosol and is required for the interaction with SQSTM1, and overexpression of the FB1 domain only in cytosol reduces CCCP-induced mitochondrial degradation via competitive interaction with SQSTM1. In addition, CHDH, but not the CHDH FB1 deletion mutant, forms a ternary protein complex with SQSTM1 and MAP1LC3 (LC3), leading to loading of LC3 onto the damaged mitochondria via SQSTM1. Further, CHDH is crucial to the mitophagy induced by MPP+ in SN4741 cells. Overall, our results suggest that CHDH is required for PARK2-mediated mitophagy for the recruitment of SQSTM1 and LC3 onto the mitochondria for cargo recognition.</P>

대량의 각혈로 사망한 장티푸스 1예

한상우,유진홍,권순석,김성훈,박지찬,홍은정,최경성,박용완,장준희,안지원,박유경,강지영,박상미,신완식 대한감염학회 2004 감염과 화학요법 Vol.36 No.5

We experienced a 25 year-old male patient with typhoid fever complicated with massive hemoptysis. Pulmonary complication in typhoid fever is very rare and to our knowledge, there has been no report of hemoptysis as a main cause of death with this disease. We herein report a rare case of typhoid fever.

고관절 반 치환술의 대퇴 골절 예방을 위한 강선 고정의 효과

유정한 ( Jeong Han Yoo ),박용욱 ( Yong Wook Park ),박진수 ( Jin Soo Park ),노규철 ( Kyu Cheol Rowe ),정국진 ( Kuk Jin Chung ),김홍균 ( Hong Kyun Kim ),김형년 ( Hyong Nyun Kim ),임희준 ( Hee Joon Lim ),이철 ( Cheol Lee ),황지효 ( 대한고관절학회 2009 Hip and Pelvis Vol.21 No.2

목적: 무시멘트형 양극성 반 인공 관절 치환술에 있어서 수술 도중 발생하는 대퇴 골절 예방을 위한 예방적 강선 고정술의 효과를 분석하고자 한다. 대상 및 방법: 2004년 1월부터 2008년 8월까지 무시멘트형 양극 인공 관절 치환술을 받은 65세 이상의 환자 48명(1군)을 대상으로 하여 강선을 사용하지 않은 48명의 환자(2군)와 비교하였다. 평균 나이는 75.4세(1), 73.6세(2)이었고 평균 추시 기간은 19.1개월(1), 18.3개월(2)이었다. 수술 도중 골절 발생율 외에 수술 시간, 수술 도중 예상 출혈량, 입원 기간, 방사선학적 안정성을 측정하였고 임상적 평가를 위한 대퇴 통증과 Jensen의 기능 점수와 Parker와 Palmer의 활동 점수를 비교하였다. 결과: 1군에서는 수술 도중 골절이 발생하지 않았으며 2군에서 4예(8.3%)가 발생하였다(p=0.041). 수술 시간은 각각 172분(1), 162분(2) 예상 출혈량은 866 cc(1), 855 cc(2), 입원기간 36일(1), 35일(2)이었다. 방사선학적으로 침강은 1.59 mm(1), 1.67 mm(2)이었으며 임상적으로 대퇴 통증이 1예(1), 2예(2) 관찰되었다. 임상적으로 Jensen의 기능 점수는 평균 2점(1) 2.2점(2), Parke와 Palmer의 활동 점수는 평균 5.2점(1), 5.3점 (2)이었다. 결론: 무시멘트형 인공 관절 치환술에 있어서 예방적 강선 고정술은 골절률을 의의있게 감소시켰다. Purpose: The purpose of this study was to assess the effect of a prophylactic cable fixation for prevention of femoral fractures in cementless bipolar hemiarthoplasties. Materials and Methods: Forty-eight cementless bipolar hemiarthroplasties with prophylactic cable fixations on the femur were performed in patients>65 years of age between January 2004 and August 2008 (group 1). The control group which did not undergo prophylactic cable fixation included 48 cases (group 2). The mean age was 75.4 years (group 1) and 73.6 years (group 2). The patients were followed up for an average of 19.1 (group 1) and 18.3 months (group 2). The intra-operative fracture rates were compared. Additionally, operative time, estimated blood loss (EBL), and length of hospital stay were compared. Radiologic assessment for stem stability was performed. Clinical assessment was evaluated by the thigh pain and Jensen`s functional score, and Parker & Palmer`s mobility score. Results: There was no fractures in the cabled group (1), and 4 fractures (8.3%) in the control group (2; p=0.041). The mean operative time was 172 minutes (1) and 162 minutes (2), the EBL was 866 cc (1) and 855 cc (2), and the duration of admission was 36 (1) and 35 days (2), respectively. Radiologically, subsidence was 1.59 mm (1) and 1.67 mm (2). Clinically, one (1) and two (2) thigh pains were recorded and the functional score of Jensen was 2 (1) and 2.2 points (2), and the mobility score of Parker and Palmer was 5.2 (1) and 5.3 points (2), respectively. Conclusion: Prophylactic cable fixation is effective for reducing intra-operative femoral fractures.

유동성 레진 수복술에서 접착제 중합 여부에 따른 미세누출과 전단결합강도에 관한 연구

박용규,김종수,유승훈 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.3

光州·全南地域 輸出産業의 競爭力 提高方案에 관한 硏究

劉冕式,鄭溶基,朴光淳 全南大學校企業經營硏究所 1992 産業經濟硏究 Vol.15 No.1

복잡한 배경에서 얼굴 영역 추출

박용성,유태웅 群山大學校 1996 論文集 Vol.23 No.-

Finding human faces automatically in a scene is a difficult yet significant problem. This paper proposes a method for detecting face regions from a complex background, and describes the experimental results. We perform face location based on the observation that human faces are characterized by their color information and texture features as follows. Firstly we construct a face chromatic histogram in hue and saturation chromatic space. Secondly we use the chromatic histogram to detect skin pixels from the input image. Thirdly a face texture model is constructed by calculating inertia, inverse difference and correlation of facial images. Finally the locations of the face region are determined by face texture features.

Indium 添加된 CdS/(p)Si 異種接合 太陽電池의 製造 및 特性에 관한 硏究

朴鏞寬,金正浩,李載亨,兪榮植 成均館大學校 科學技術硏究所 1995 論文集 Vol.46 No.1

In this study, the CdS/(p)Si solar cell was fabricated by electron-beam evaporation method, and their properties were investigated. Also the resistivity and optical transmission of CdS thin film were measured. The relation between this results and solar cell characteristics was considered, and came to as follow conclusion. 1. CdS this film is the polycrystalline in direction of (002), and has the hexagonal structure. In proportion to increase of substrate and annealing temperature, the optical band gap, and therefore the photo-transmittance in the absorption edge increases, while conductivity above the critical conditions decreases. 2. In proportion to Indium concentration, the resistivity of CdS thin film is the lowest when Indium concentration is 2[wt%]. In the photo-transmittance for the whole visible light, when the concentration is 2 [wt%], Burstein- Moss shift is the largest. 3. I_SC, V_OC FF, η, display the maximum value when the annealing temperture is at 350[℃]. 4. The characterisitcs of solar cells, that is I_SC V_OC FF, η, fabricated by mixture with In, somewhat more increases than those which is not mixed with. 5. In the optimal conditions the I-V characterisitics of the fabricated CdS/(p)Si solar cells shows as follows. V_OC:450[mV], I_SC:11.5[mA/㎠'], FF:0.475, η:4.92[%]

담낭질환의 Neuron Specific Enolase 반응세포 출현에 관한 면역조직화학적 연구

박용욱,박언섭,유재형 중앙대학교 의과대학 의과학연구소 1992 中央醫大誌 Vol.17 No.1

A total 50 patients who had undergone cholecystectomy for cholecystopathy were included in the study to observe the staining pattern of the cells and distribution of antihuman Neuron Spectific Enolase (NSE) antibody by avidin-biotin immunoperoxidase method. The studies consisted of 31 cased of chronic cholecystitis with lithiasis, 9 cases of adenoma and 10 cases of carcinoma including 6 cases of moderately differentiated adenocarcinoma, 3 cased of poorly differentiated adenocarcinoma, and 1 case of small cell carcinoma of combined type. The results were as follows : 1. In the chronic cholecystitis with lithiasis, nine of 31 cases (29%) with the NSE immunoreactive cells, arranged in single of grouped fashion, are focally observed in the covering surface epithelium. 2. The NSE immunoreactivities of carcinoma is more stronger than that of chronic cholecystitis and adenoma. 3. The coarse granular intracytoplasmic strong positive reaction of small cell carcinoma is diffusely observed in noeplastic small cell and glandular epithelium. Therfore, authors suggested that two elements arise from a common primitive cells of endodermal derivation capapble of differentiation along two cell-endocrine ans epithelial.

프레탈정 (실로스타졸 100mg)에 대한 실로스탄정 (한국유나이티드 제약)의 생물학적 동등성

용철순,이경희,최진석,박병주,정세현,김용일,박상만,유봉규,이종달,최한곤 한국병원약사회 2003 병원약사회지 Vol.20 No.2

Bioequivalence of one cilostazol tablets, the Pletaal^(R)(Korea Otsuka Pharmaceutical Co., Ltd.) and Cilostan^(R)(Korea united Pharmaceutical Co., Ltd.), was evaluated according to the guideline of KFDA. Sixteen normal male volunteers(age 20~30 years old)were divided into two groups and a randomized 2×2 cross-over study was employed. After one tablet containing 100㎎ of cilostazol were orally administered, blood was taken at predetermined time intervals and the concentration of cilostazol in plasma was determined with an HPLC method using UV detector. The pharmacokinetic parameters(C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameter. The results showed that the differences in C_(max), T_(max) and AUC_(t) between one tablet were 16.08%, 18.88% and 17.57%, respectively. The powers (1-β) for C_(max), T_(max) and AUC_(t) were 85.03%, 83.92% and 80.12%, respectively. Detectable differences(Δ) and confidence intervals were all less than 20%, and confidence interval of all the parameters were also less than 20% at the significance level(α) of 0.05. All of these parameters met the criteria of KFDA for bioequivalence, indicating that Cilostan^(R) tablet is bioequivalent to Pletaal^(R) tablet.

브러시레스 DC 모타의 最大起動 토오크를 위한 Hall Effect Sensor와 스테이터 코아 間의 最適配置

權鏞寬,兪榮植,李尙燁 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.2

This paper describes how to arrange a hall effect sensor of brushless DC motor for the maximum starting torque and low torque ripple. To decide the optimal positioning of a hall effect sensor, the effect of time constant and hysteresis on hall effect sensor is considered. The 66° (E) is calculated as the optimal positioning between a hall effect sensor and a stator core for the maximum starting torque. But 68° (E) is demonstrated as the real positioning for the maximum starting torque through the test.