Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells

( Jae-sung Ryu ),( Kyu-tae Chang ),( Ju-taek Lee ),( Malg-um Lim ),( Hyun-ki Min ),( Yoon-ju Na ),( Su-bin Lee ),( Gislain Moussavou ),( Sun-uk Kim ),( Ji-su Kim ),( Kinarm Ko ),( Kisung Ko ),( Kyung- 생화학분자생물학회 2017 BMB Reports Vol.50 No.6

The ACKNOWLEDGEMENTS should be corrected as follows, This research was supported by a grant (Code# PJ0074922012) from the Korean Rural Development Administration, and has been supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education Science and Technology (2010-0022316 and KCG5401011), and the KRIBB/KRCF Research Initiative Program (NAP), Korea. The published article in this journal was a part of the 2013 Doctoral thesis of Jae-Sung Ryu submitted to Wonkwang University, Korea. and not This research was supported by a grant (Code# PJ0074922012) from the Korean Rural Development Administration, and has been supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education Science and Technology (2010-0022316 and KCG5401011), and the KRIBB/KRCF Research Initiative Program (NAP), Korea.

식이섬유 보충용 식품의 섭취에 따른 여성의 비만관련 체구성 요인 및 배변만족도의 변화

임용택,강선영,김재등,김성수,김재우,이충영,김정모 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.1

The Purpose of this study was to investigate the effect of functional food including dietary fiber supplementation on body composition concerning obesity and defecation satisfaction of women. Twelve young healthy women but had defecation difficulty were participated as a subject. Body weight, %body fat, WHR mesurement and defecation satisfaction questionnaire were performed before and after 2 weeks' supplementation. The results of this study were as follows : First, Body composition concerning obesity such as body weight(p=.0065), % body fat(p =.0004) and WHR(p=.0007) after 2 weeks' functional food including dietary fiber supplementation was decreased significantly. Second, defecation satisfaction functional food including dietary fiber supplementation was increased significantly(p = .0039). This results suggested that functional food including dietary fiber supplementation should be recommended as effective dietary food for persons with obesity and defecation difficulty.

구리 지역에서 조사한 고혈압의 인지도, 치료률 및 관리률

홍철호,김순길,홍택원,이제,신진호,이재웅,김경수,김정현,임헌길,이방헌 대한간질학회 2003 대한간질학회지 Vol.9 No.1

쇄골 중간부 골절후 불유합 및 지연 유합의 원인

윤정로,심재익,김택선,이성종,김영배,김학준,안국환,장재영,홍명표 대한골절학회 2002 대한골절학회지 Vol.15 No.4

쇄골 중간 1/3 부위의 골절은 치료 결과가 양호하여 보존적 치료가 원칙적으로 받아 들어지고 잇으며 불유합에 대한 빈도도 낮은 것으로 되어 있다. 그러나 최근 전위 및 단축의 정도가 심할 경우 높은 불유합의 결과와 불량한 임상 결과를 보고하고 있어 이런 예에 대해선 수술적 치료를 권장하고 있다. 저자들은 단축 및 전위의 정도와 불유합의 연관성을 알아보고자 하였다. 연구 대상 및 방법 : 1993년 2월부터 2002년 1월까지 본원에 내원 했던 성인 중간부 쇄골 골절 환자 194명 중 완전 전위를 보인 78례를 조사하여 이중 63례를 대상으로 분석하여 후 향적 연구를 시행하였다. 4개월 추시 후에도 방사성 사진상 유합 소견이 관찰되지 않으면 불유합의 유의성을 알아보고자 하였다. 결 과 : 63례 중 불유합 및 지연 유합을 소견을 보인 환자는 15(23%)례였다. 골유합을 보인 48례의 단축은 평균 8.6㎜(2㎜-17㎜)였고, 전위는 평균 9.7㎜(2-22㎜)였다 .불유합 및 지연 유합을 보였던 15례에서 단축은 평균 14.5㎜(3㎜-37㎜), 전위는 평균 17.3㎜(4-25㎜)였다. 18㎜이상 단축이 있었던 경우 불유합의 발생과는 통계학적으로 유의성을 나타내었고(Fisher's exact test, p<0.01), Chi-square test상 16㎜ 이상의 전위를 보였던 경우 불유합 발생과 통계학적으로 의미가 있었다(p<0.01). 결 론 : 쇄골 골절 특히 중간 1/3의 골절은 비교적 보존적 치료에 결과가 좋은 것으로 되어 있으나, 전위 및 단축이 심한 경우 불유합에 대한 가능성이 높이 때문에 보다 경과 관찰시 유합의 진행이 없으면 적극적인 치료를 고려해야한다. Purpose : Because the prognosis of the mid 1.3 clavicle fracture is good, the conservative treatment with a figure of 8 bandage is the gold standard and the nonunions are rare. However, recently surgical treatment is recommended when the shortening and displacement is severe because of the high nonunion rate and the poor clinical result. This study was undertaken to evaluate that the shortening and displacement at fracture site are associated with the development of nonunion. Materials and Methods : We analysed the 194 fractures of mid 1/3 clavicle in adults which had been treated conservatively from February 1993 to January 2002 and did the retrospective study. Of these, 78 cases were originally in the middle third of the clavicle and had been completely displaced. We reviewed 63 of these cases. The shortening and displacement at the fracture site was measured on the initial roentgenogram. And the analysis of the patients' chart was done for another predisposing nonunion factors. Nonunion and delayed union are considered to be present when there has been little or no progression of clinical or radiographic healing at a minimum of 4 months after injury. Results : 15 of the 63 cases had developed nonunion.. The average 8.6㎜(2㎜-17㎜) shortening and average 9.7㎜(2-22㎜) in the union patients. The average 14.5㎜(3㎜-37㎜) shortening and average 17.3㎜(4-25㎜) in the nonunion patients. We found that initial shortening ≥18㎜(Fisher's exact test, p<0.01) and initial displacement ≥ 16㎜(Chi-square test. p<0.01)at the fracture site were significantly associated with the development of nonunion. Conclusion : The conservative treatment with figure-80-bandage is the gold standard in the clavicle middle one third fracture. However, the nonunion is commonly occurs in the cases of more of severely shortened and displaced fractures. If there are no signs of callus formation and the patient complains of pain after several week, osteosynthesis should be considered.

코스닥 등록기업의 회계정보가 기업지분가치에 미치는 영향

김영택,김재문 한국세무회계학회 2002 세무회계연구 Vol.11 No.-

본 연구는 Ohlson모형과 이를 확장한 모형을 이용하여, 우리 나라 코스닥 등록기업의 회계정보가 기업지분가치에 영향을 미치는지에 대하여 실증분석을 실시하였다. 표본기업은 1999년부터 2001년까지의 12월 결산기업 중에서 일반기업 90개, 벤처기업 72개 등 총 162개였다. 본 연구의 실증분석결과를 요약하면 다음과 같다. 첫째, 단순한 Ohlson모형의 분석결과, 일반기업은 배당금(D), 전체에서는 장부가치(BV)와 배당금(D)이 기업지분가치(ME)에 영향을 미치는 것으로 나타났다. 둘째, 개발비(IPR)와 산업재산권(DC)이 기업지분가치평가에 미치는 영향을 검증하기 위한 분석결과, 일반기업은 개발비와 산업재산권을 제외한 장부가치(BVLDI), 산업재산권(DC), 개발비(IPR), 회계이익(X), 벤처기업에서는 BVLDI, 전체에서는 BVLDI, 개발비(IPR), 배당금(D)이 기업지분가치(ME)에 영향을 미치는 것으로 나타났다. 셋째, 경상연구개발비(OP)와 광고선전비(AD)는 일반기업, 벤처기업, 그리고 전체기업 모두에서 기업지분가치에 영향이 미치지 않는 것으로 나타났다.

레이저 용융 적층공정을 이용한 정밀 형상 제작

김재도,전병철,권택열,이영곤,신동훈 한국레이저가공학회 2000 한국레이저가공학회지 Vol.3 No.3

For the direct metal shape processing the powder feed device which is different from the widely used in rapid prototyping. Is developed, The three dimensional object is shaped with the melting metal powder. The developed research has applied to rapid prototyping in ultraprecision for MEMS and medical science fields required of rapid manufacture of complex shape. The goal of this study make 3D model which has precision accuracy. Powder spreading apparatus has been more improved because that the control of powder spread is very important in layer manufacturing. It consists of the vibration motor, nozzle and tube which supplies various metal powder. This apparatus could control the spreading velocity that could control powder spreading thickness. Laser on/off switch was adapted because laser scanning velocity must be preserved constantly to prevent heat transformation of laser overheating. The error between sintered thickness and experimental one occurred by shrinkage in sintering melting process. The problem of heat transformation was solved by On/Off switching system.

순막조건화에서의 소뇌 피질의 기능

김현택,류재욱,김기석 한국심리학회 2002 한국심리학회지 생물 및 생리 Vol.3 No.1

토끼 순막조건화에 관여하는 소뇌피질의 기능을 검토하고자 소뇌의 심부핵중 하나인 중간핵 그리고 소뇌피질 단소엽을 연구대상으로 선정하였다. 실험 1에서는 전통적 학습형태와 대조적 학습형태인 청각자극 무제시와 무조건자극의 배쌍이 조건반응을 일으키는지의 여부를 알아보았다. 이러한 배쌍에서도 토끼는 신속한 조건반응을 획득하였는데 이 결과는 소뇌피질의 학습형성기전에 대한 기존의 설명 즉 조건자극제시에 의한 태상섬유 활동 증가와 무조건자극제시에 의한 등상섬유 활동증가가 태상섬유활동을 전해 받는 과립세포의 축색인 평행섬유와 퍼킨지세포사이의 시냅스 변화를 일으켜서 조건반응을 나타나게 한다는 설명과는 부합되지 않는 것이다. 실험 2에서는 소뇌피질의 반응시간조절기능가설을 검증하기 위하여 조건자극의 기간을 전형적 조건화 지수의 2배인 800msec로 하였다. 그리하여 소뇌피질의 손상이 손상후 시행의 조건자극제시기간동안의 조건화된 순막반응에 어떤 변화를 초래하는지 관찰하기 용이하게 하였다. 피험동물은 손상후 조건반응에서 부분적 폐지만을 보였는데 이는 전반적인 조건반응진폭의 축소에서 기인한 것이 아니라 손상후 회기에서 조건반응 개시 시각의 큰 변산에서 기인한 결과임을 관찰하였다. 이것은 소뇌피질이 중간핵과 기능적 연관성을 가지며 조건반응출현의 시간조절역할을 한다는 가설을 지지해 주는 결과라 하겠다. The cerebellar lobule HVI was investigated in terms of plastic structure mediating the classical conditioning of the rabbit's nictitating membrane response(NMR). In experiment 1, animals were received tone stimulus(80㏈, 1㎑, sine wave, without white noise) continually during the intertrial interval(ITI). The tone stimulus was turned off during the CS period. This withdrawal of tone stimulus was adopted as CS^(-). Animals received the CS^(-) and corneal airpuff US parings in delay conditioning paradigm rapidly reached criterion like animals received CS-US pairings. Moreover, the electrolytic leisions of the interpositus nucleus selectively disrupted the CR aquired by CS^(-)-US pairings. The result suggests that this type of conditioning is mediated by the cerebellum as well and the cerebellum can associate the changes of stimuli itself. In addition, there was no transfer effect between CS^(-)-US and CS-US learning. The findings are discussed in terms of the convergence of the climbing and the mossy fibers on the Purkinje cells and the associative functions of the cerebellar neural network. In experiment 2, the long ISI was adopted(800msec CS duration, delay conditioning paradigm) in order to investigated the timing regulation function of the cerebellar cortex on motor learning. After the NMR conditioning(CS^(-)-US, group1 and CS-US, group2) reached the criterion, the cerebellar lobule HVI was electrolytically lesioned with four electrodes. The result showed that the CR was reduced about 50% in both groups and the effects were due to the disruption of timing regulation of the CR onset. Results indicated that the cerebellar lobule HVI is essential for the timing regulation of the CR onset. However, to understand the detailed neural mechanism of the conditioning of NMR, it was suggested that neural network approach including several structures resided in the cerebellum and in the brain stem(especially inferior olive) is more desirable than finding only one essential structure approach.

Consideration of Diluents Selection and Input Amounts of the Hunter Process for Tantalum Production

Jae‑Jin Sim,Sang‑Hoon Choi,Yong‑Kwan Lee,Sung Gue Heo,Taek‑Soo Kim,Seok‑Jun Seo,Kyoung‑Tae Park 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.7

Tantalum (Ta) is a heavy refractory metal with an atomic weight of 180.95 g/mol, a density of 16.6 g/cm3, and a high meltingpoint of 3017 °C. With its refractory characteristics, it shows excellent chemical and physical stability as well as corrosionand heat resistance at elevated temperatures. The demand for Ta metal and related compounds is increasing with the expansionof the electronics and chemical industries. The Hunter process was proven to be effective in producing Ta powder in1953. Hunter proposed a method in which potassium heptafluorotantalate (K2TaF7) was reduced by sodium (Na). Thus far,this process has been the primary commercial method to produce Ta powder. In this study, quantitative differences wereanalyzed for diluent selection. Additionally, consideration was given to changes in the caloric value depending on the inputamount of diluents. Finally, the optimum material input and the properties of the prepared Ta were analyzed. Stoichiometricratios of K2TaF7(1 mol), NaCl (6.2–6.7 mol), Na (5–7 mol) were weighed, to perform a metallothermic reduction reaction. After the reaction, the tantalum powder was recovered and the flush process was carried out. After that, it was dried in avacuum atmosphere. Physical properties such as oxygen concentration, PSA, ICP-OES, and XRD of powder were conductedto evaluate the characteristics of Ta powder that was finally manufactured.

Bubble Cap Tray, Value Tray 및 Seive Tray를 이용한 알콜/물 분리

김석택,최동훈,김재훈,김장호,빌렛 라인하르트 동아대학교 환경문제연구소 2000 硏究報告 Vol.23 No.1

In this study The diagrams for various packing shown given an impression of the effectiveness of several fraction device for separation towers in alcohol distillation. Although not claiming completeness, they are yet indicative of the influence which the characteristic design and operating parameters are having on the performance efficiency of the internals. The relevant technical literature provides comprehensive information for fluid dynamic computations as well as for predetermining the separation efficiency of columns working under industrial operating conditions, this applies to tray columns and packed columns equally. The most recent computation models of thermal separation technology allow the quantitative valuation also of other efficiency when used in industrial alcohol distillation plants, and the corresponding dimensioning of columns with an accuracy that is sufficient for the requirements of practice.

알러지성 접촉피부염 유발 피부 주변 림프절에서의 세포성 면역 활성에 관한 면역조직화학적 연구 : T 림프구와 IL-2 수용기의 분포 변화를 중심으로 Based on the change of T lymphocytes and Il-2 receptors

김진택,안상현,박인식,정재만,김호현 동국대학교 한의학연구소 1998 東國韓醫學硏究所論文集 Vol.7 No.1

본 연구는 알러지성 접촉피부염(allergic contact dermatitis) 유발 피부 주변 림프절에서의 세포성 면역활성으로 나타나는 면역조직화학적 형태변화를 조사하기 위해 DNCB로 인위적인 알러지성 접촉피부염을 BALB/C계 생쥐의 샅바위부분 피부에서 유발시킨 후 시간 경과에 따른 샅바위 림프절(inguinal Iymph node)에서의 T 림프구와 IL-2 수용기의 분포변화를 관찰하였다. 대조군에서는 L3T4(CD4)에 양성반응을 보이는 도움 T 림프구, Ly2(CD8)에 양성반응을 보이는 세포독성 T 림프구 그리고 CD25R에 양성반응을 보이는 IL-2 수용기를 가진 세포는 곁피질(paracortex)과 수질동(medullary sinus)에서 분포하였다. DNCB에 의한 알러지성 접촉피부염 유발후 24시간부터 도움 T 림프구, 세포독성 T 림프구 그리고 IL-2 수용기를 가진 세포가 곁피질과 수질동에서 증가하기 시작하여 48시간에 이르러서는 그 분포와 양성반응성이 최고에 달했다. 48시간의 이러한 분포는 수질동에서 잘 나타났으며, 특히 세포독성 T 림프구가 많은 증가를 보였다. 72시간에 이르러서는 양성반응세포가 서서히 감소되는 것으로 나타났지만, 대조군에 비해서는 여전히 증가된 분포양상으로 나타났다. 이상의 결과로 미루어보아 DNCB에 의한 접촉성 피부염 유발시 림프절에서는 도움 T림프구의 분열·활성 증대로 인한 IL-2 생성·분비 증가의 결과 세포독성 T 림프구의 분열·활성 유도하는 일련의 세포성 면역연쇄반응의 활성이 일어나게 된다. 이러한 세포성 면역연쇄반응의 활성은 주변 피부에서 일어나는 알러지성 접촉피부염으로 인한 피부손상을 주도하는 것으로 사료된다. Lymph node tissues of BALB/C mouse treated with DNCB were immunohistochemically observed to investigate the activation of cell mediated immunity in Iymph node of murine with allergic contact dermatitis. The inguinal region of BALB/C mice were sensitized by one application of 25㎕ of 5% 2,4·-dinitrochlorobenzene(DNCB) onto an abdominal skin and 2 weeks later, the mice were challenged with 4㎕ of 2.5% DNCB. The inguinal lymph node were obtained at hour 24, 48, and 72 after 2nd DNCB treatment and embedded with paraffin, and then stained by following ABC method that used monoclonal antibody including L3T4(CD4), Ly2(CD8), IL-2R(CD25). The distribution of helper T lymphocytes, cytotoxic, T lymphocytes and IL-2 receptors began to increase at hour 24 after 2nd DNCB treatment and these increase appeared in paracortical area and medullary sinius. These increase were greatest at hour 48. These results indicated that the IL-2 secretion began to increase by activation of helper T lymphocytes in lymph node of DNCB re-exposure area and subsequently to activate suppress T lymphocytes.