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영구치 치수 기질세포를 이용한 연골 분화 및 분화 시기에 따른 형태학적 변화
정주령,김하나,박열,김민정,오영주,신수정,최윤정,김경호 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.1
Objectives: The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods: Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results: Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions: Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation.
대학생의 우울정도 : 간호학전공과 타전공대학생을 중심으로
김지연,류나은,이소라,이지희,정윤정,주지연,황인혜 이화여자대학교 간호과학대학 2012 이화간호학회지 Vol.- No.46
Purpose: This study was designed to investigate sample’s general characteristics and compare depression degree among sample’s general characteristics and students’ major(nursing vs. other major). Methods: The subjects consisted of 318 university students. Data was collected by self-reported questionnaires, which were constructed BDI score. Data was analyzed by the SPSS/PC WIN 19.0 program. Results: The depression of nursing students and other major students was not significantly different according to BDI score. Statistically significant difference was identified among sleeping. Conclusion: Specific study focused on the practice time should be done to confirm the depression of nursing major and other majors. Detailed support programs which specifically deal with sleeping should be developed to effectively reduce the harmful effects of individual vulnerability. Therefore, prevention and management system to reduce depression degree for university students is needed.
( Ju Yup Lee ),( Na Young Kim ),( Yoon Jeong Choi ),( Ryoung Hee Nam ),( Yoon Jin Choi ),( Yong Hwan Kwon ),( Kichul Yoon ),( Hye Seung Lee ),( Dong Ho Lee ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Helicobacter felis mouse model has been developed for the research regarding pathogenesis of chronic gastritis and gastric cancer. The aim of this study was to investigate long-term H. felis colonization in the stomachs of C57BL/6 mice and subsequent histologic fi ndings and infi ammatory reactions including pro-infi ammatory cytokines. Methods: Twenty-three female C57BL/6 mice at 4 weeks of age were gavaged with H. felis, and 13 control mice served as vehicle only. The mice were sacrifi ced at 4, 24, and 52 weeks after inoculation. The infection status and degree of infi ammation were determined by culture and histopathology. The level of gastric mucosal myeloperoxidase (MPO), tumor necrosis factor alpha (TNFa), and interleukin-1beta (IL-1ß) were measured by ELISA. Results: The overall infection rate was 100%, as determined by the culture and histology. At 4, 24, and 52 weeks, the neutrophil and monocyte scores were signifi cantly higher in infected mice than in control mice. At 24 weeks after inoculation, most of the infected mice showed mucosal atrophy with or without metaplasia, and a few showed focal dysplasia. Adenocarcinoma was observed in one mouse at 52 week post-infection (Table). Gastric mucosal MPO and IL-1ß levels were signifi cantly higher in infected mice than control mice at 24 and 52 weeks, however, the expression of gastric mucosal TNFa was not signifi cantly different between the infected and control mice at any time-point. Conclusions: Long-term H. felis-infection in C57BL/6 mice provoked a severe infi ammatory reaction and it progressed into atrophy, metaplasia, dysplasia and cancer. IL- 1ß might play an important role in the infi ammatory response of mice to Helicobacter species.
Ju-Taek Lee,Jae-Sung Ryu,Ghislain Moussavou,Malg-Um Lim,Hyun-Ki Min,Yoon-Ju Na,Su-Bin Lee,In-Sun Kim,Ju-Hyoung Lee,Ji-Su Kim,Sun-Uk Kim,Kyu-Tae Chang,Young-Kug Choo 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
Glycosphingolipids including gangliosides play important regulatory roles in cell prolifera-tion and differentiation. UDP-glucose:ceramide glucosyltransferase(Ugcg) catalyze the initial step in glycosphingolipids biosynthesis pathway. In this study, Ugcg expression was reduced to approximately 80% by short hairpin RNAs(shRNAs) to evaluate the roles of glycosphingolipids in proliferation and gangliosdies differentiation of PK15. HPTLC/immunofluorescence analyses of shRNA transfected PK15 revealed that treatment with Ugcg-shRNA decreased expression of major gangliosides, GM1 and GD1b. Furthermore, MTT and western blot/immunofluorescence analyses demonstreated that inhibition of the Ugcg expression in PK15 resulted in decrease of cell proliferation. In addition to we showed that inflammatory respon-ses in PK15 and gangliosides knock-down PK15 cells stimulated with Escherichia coli lipopolysaccharide(LPS). The gangliosides GM3, GM2, GD3, GD1a and GD1b were detected in normal PK15 cell. However almost gangliosides undetected in gangliosides knock-down PK15 cell. In addition we found that the expression levels of TNF-α, indu-cible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and we measured the production of NO. The expression of inflammatory proteins, TNF- α, iNOS and COX-2 the elevated concentration in gangliosides knock-down PK15 cell. These results suggest that gangliosides interact with components of the proinflammatory response pathway and might, therefore, be relevant for designing future therapeutic strategies intended to prolong xenograft survival.
( Yoon Ju Na ),( Ki Nam Shim ),( Seong Eun Kim ),( Hye Kyung Jung ),( Min Sun Cho ),( Sung Ae Jung ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Helicobacter pylori ( H. pylori) cytotoxin-associated gene A (CagA) has been suggested to be involved in the inactivation of Runt-related transcription factor 3 (RUNX3), a known gastric cancer tumor suppressor gene. It remains unclear how H. pylori CagA initiates or maintains RUNX3 promoter methylation and inactivates its protein expression in gastric cancer. Methods: Promoter methylation status and RUNX3 expression was investigated in 76 sample pairs of gastric cancer tissue. The patients` medical records were reviewed. The association between RUNX3 methylation or loss of RUNX3 expression and clinicopathologic variables according to H. pylori CagA status were investigated.Results: There was no relationship between H. pylori CagA infection and RUNX3 methylation. In patients with H. pylori CagA-positive gastric cancer, RUNX3 methylation was not associated with lymphtic invasion, venous invasion and TNM stages. But RUNX3 methylation was observed more frequently in poorly differentiated and signet ring cell carcinoma (P = 0.023) in early gastric cancer. In the H. pylori CagA-negative gastric cancer, patients without RUNX3 methylation showed lymphatic invasion (P = 0.047), venous invasion (P = 0.046), and lymph node metastasis (P = 0.012) were more frequently than those with RUNX3 methylation. In patients with H. pylori CagA- positive gastric cancer, loss of RUNX3 expression was not associated with lymphic invasion, venous invasion and TNM stages. Conclusion: In patients with H. pylori CagA-positive, RUNX3 methylation or loss of RUNX3 expression did not correlated with lymphovascular invasion and TNM stages. In patients with H. pylori CagA-positive early gastric cancer, RUNX3 methylation was observed in the poorly differentiated or signet ring cell carcinoma. Further study for the association between H. pylori CagA and RUNX3 methylation or loss of RUNX3 expression in early stage of gastric cancer were needed.
위장관 ; 위암에서 Helicobacter pylori CagA에 따른 RUNX3의 메틸화 및 발현의 소실과 임상병리학적 특성과의 관계
나윤주 ( Yoon Ju Na ),심기남(교신저자) ( Ki Nam Shim ),주양희 ( Yang Hee Joo ),김성은 ( Seong Eun Kim ),정혜경 ( Hye Kyung Jung ),정성애 ( Sung Ae Jung ),조민선 ( Min Sun Cho ) 대한소화기학회 2015 대한소화기학회지 Vol.66 No.2
Background/Aims: Helicobacter pylori cytotoxin-associated gene A (CagA) has been suggested to be involved in the inactivation of Runt-related transcription factor 3 (RUNX3), a known gastric carcinoma tumor suppressor gene. It remains unclear how H. pylori CagA initiates or maintains RUNX3 promoter methylation and inactivates its protein expression in gastric carcinoma. Methods: RUNX3 promoter methylation status, RUNX3 expression, and H. pylori CagA were investigated in 76 sample pairs of gastric carcinoma tissue. The patients’ medical records were reviewed. The association between RUNX3 methylation or loss of RUNX3 expression and clinicopathologic variables according to H. pylori CagA status were investigated. Results: In gastric carcinoma patients with H. pylori CagA-positive infection, RUNX3 methylation did not show association with lymphatic invasion, venous invasion, and TNM stages. However RUNX3 methylation was observed more frequently in poorly differentiated adenocarcinoma and signet ring cell carcinoma (77.8% vs. 20.0%, p=0.023) in early stage. In gastric carcinoma patients with H. pylori CagA-positive infection, loss of RUNX3 expression did not show association with lymphatic invasion, venous invasion, and TNM stages. However loss of RUNX3 expression was observed more frequently in early gastric carcinoma than in advanced gastric carcinoma (84.2% vs. 75.0%, p=0.51), but this difference was not significant. Conclusions: In gastric carcinoma patients with H. pylori CagA-positive infection, RUNX3 methylation or loss of RUNX3 expression did not show correlation with lymphovascular invasion and TNM stages. In early gastric carcinoma patients with H. pylori CagA-positive infection, RUNX3 methylation was observed more in poorly differentiated adenocarcinoma and signet ring cell carcinoma. (Korean J Gastroenterol 2015;66:75-84)
Yoo, Jeong-Ju,Yu, Su Jong,Na, Juri,Kim, Kyungmin,Cho, Young Youn,Lee, Yun Bin,Cho, Eun Ju,Lee, Jeong-Hoon,Kim, Yoon Jun,Youn, Hyewon,Yoon, Jung-Hwan MDPI 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.6
<P>This study aimed to examine whether inhibition of hexokinase (HK)-II activity enhances the efficacy of sorafenib in in-vivo models of hepatocellular carcinoma (HCC), and to evaluate the prognostic implication of HK-II expression in patients with HCC. We used 3-bromopyruvate (3-BP), a HK-II inhibitor to target HK-II. The human HCC cell line was tested as both subcutaneous and orthotopic tumor xenograft models in BALB/c nu/nu mice. The prognostic role of HK-II was evaluated in data from HCC patients in The Cancer Genome Atlas (TCGA) database and validated in patients treated with sorafenib. Quantitative real-time PCR, western blot analysis, and immunohistochemical staining revealed that HK-II expression is upregulated in the presence of sorafenib. Further analysis of the endoplasmic reticulum-stress network model in two different murine HCC models showed that the introduction of additional stress by 3-BP treatment synergistically increased the in vivo/vitro efficacy of sorafenib. We found that HCC patients with increased HK-II expression in the TCGA database showed poor overall survival, and also confirmed similar results for TCGA database HCC patients who had undergone sorafenib treatment. These results suggest that HK-II is a promising therapeutic target to enhance the efficacy of sorafenib and that HK-II expression might be a prognostic factor in HCC.</P>