In vitro Mammalian Chromosomal Aberration Test of Fullerene-C60

Soo Jin Kim(김수진),Kyung Taek Rim(임경택),Hae Won Cho(조해원),Jeong Hee Han(한정희),Hyeon Yeong Kim(김현영),Jeong Sun Yang(양정선) 환경독성보건학회 2009 환경독성보건학회지 Vol.24 No.1

Fullerene의 유전독성을 평가하기 위하여 Chinese hamster유래의 난소유아세포(CHO-K1 cell)를 이용하여 직접법(-S9)과 대사활성화법(+S9 mix)의 염색체이상시험을 실시하였다. 시험물질은 1% CMC 나트륨염의 현탁액(l% CMC 용액)에 희석하여 조제하였다. 대사활성화를 시키지 않은 직접법의 염색체이상시험에서 24시간 투여군은 8단계의 농도(0.078, 0.156, 0.313, 0.625, 1.25, 2.5, 5, 10mM)로 투여하여 실시하였다. 투여 농도 증가에 따른 염색체이상의 빈도가 증가하는 양상이 나타나지 않았다. 48시간의 투여군에서는 8단계의 농도(0.078, 0.156, 0.313, 0.625, 1.25, 2.5, 5, 10mM)로 투여하여 실시하였는데 투여 농도 증가에 따른 염색체이상의 빈도가 증가하는 양상이 나타나지 않았다. 배수체의 염색체이상은 직접법에서 관찰되지 않았다. 대사활성화법을 이용하여 6시간 시험물질을 투여한 시험에 있어서는 8단계의 용량단계 (0.078, 0.156, 0.313, 0.625, 1.25, 2.5, 5, 10mM)를 설정하였는데 투여 농도가 증가함에 따른 염색체이상빈도의 증가양상이 관찰되지 않았다. 이상의 결과를 종합할 때 본 시험물질은 본 시험 조건하에서 CHO-K1세포에서 대사활성화를 시켰을 때 염색체 이상을 유발하지 않는 것으로 판단된다.

쌍태아에서 제 1태아의 분만 후 제 2태아의 지연분만 : 중례보고

김주환,서민정,유희정,노권일,조대현,박정규,이정헌,조성남,손영수 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.3

With the widespread use of fertility medications and techniques of in vitro fertilization, multiple gestation has become epidemic in modern obstetric practice. Delivery of the initial fetus in a multiple gestation usually is followed by delivery of the subsequent fetus or fetuses shortly thereafter. However in rare circumstances, the delivery is delayed for days due to disappearance of uterine contraction after delivery of the first fetus. When uterine activity ceases after the delivery of the first neonate, a policy of nonintervention may be considered in case of an immature or very premature delivery, in order to achieve a gestational age for the remaining fetus(es) more compatible with neonatal survival. We report a case of twin gestation with prolongation of the delivery interval between the twins for 46 days.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

白鼠의 無機質代謝에 미치는 칼슘과 마그네슘의 影響 : 칼슘과 마그네슘 代謝에 미치는 影響

曺秀悅,鄭在洪 嶺南大學校 環境問題硏究所 1981 環境硏究 Vol.1 No.1

This experiment was undertaken to investigate calcium and magnesium metabolism of rats fed the diet containing two levels of calcium(0.05%, 0.1%) and magnesium (0.04%, 0.0002%). Thirty-two males of Sprague-Dewley strain, 70-80g, were divided into 4 groups and each group (8 rats) was fed ad libitum for 4 weeks. Calcium and magnesium in sera, tissues and femurs were determined by atomic absorption spectrophotometry. The results obtained are summarized as follow. Net weight gain and feed efficiency ratio were decreased in the calcium and magnesium-deficient groups. The weights of liver, heart and kidney generally showed increasing tendency in the calcium-deficient group. Lung was showen to decrease in the magnesium-dificient group compared to the control group. Serum cholesterol showed increasing tendency in the groups of deficiency in calcium and calcium plus magnesium. Serum clacium and magnesium decreased in the groups of deficiency in calcium, magnesium and calcium plus magnesium. Calcium contents in liver and kindey were not affected by the dietary clacium and magnesium levels. Calcium contents in kidney were significantly increased in the groups of deficiency in magnesium levels. Calcium contents in kidney were significantly increased in the groups of deficiency in magnesium and calcium plus magnesium. Calcium contents in femur were decreased in the Calcium-deficient group, whereas magnesium contents in liver were increased in the magnesium-deficient group. Calcium and magnesiumexcretion to feces and urine was decreased in the calcium and magnesium deficient groups respectively.

白鼠의 無機質代謝에 미치는 칼슘과 마그네슘의 影響 : 2. Effect on the sodium, potassium, copper and iron metabolism in tissues 2. 組織 중의 Na, K, Cu 및 Fe 代謝에 미치는 影響

曺秀悅,鄭在洪 嶺南大學校 環境問題硏究所 1982 環境硏究 Vol.2 No.1

This experiment was undertaken to investigate the effect of the diet containing two levels of calcium(0.5%, 0.1%) and magnesium (0.04%, 0.0002%) on the sodium, potassium, copper and iron concentrations of kidney, liver and femur in rats. Thirty-two males of Sprague-Dewley strain, 70-80g, were divided into 4 groups and each group(8 rats)was fed ad libitum for 4 weeks. sodium and potassium in the liver, kidney and femur were determined by flame spectrophotometry. Copper and iron in liver, kidney and femur were determined by atomic absorption spetrophotometry. The results obtained are summarized as follow. Net weight gain and feed efficiency ratio were decreased in the calcium and magnesium-deficient groups. The weight of liver, heart and kidney generally showed increasing tendency in the calcium-deficiency group. The weight of iung was showed to decreased in the magnesium-deficient group compared to the control group. The sodium and potassium concentrations of liver, kidney, and femur are the dietary calcium and magnesium levels did not tend to affect on the sodium concentration of kidney and potassium concentration fo femur and liver. But sodium content of liver tended to be significantly increased in the magnesium deficient-group. The potassium concentration of kidney in the deficient-groups(II,III,IV) tended to be greatly increased as compared with the control group. The sodium concentration of femur in the deficient-groups(II, III, IV) was slightly lower as comapared with that of the control group. Both copper and iron contents of the kidney, liver, and femur were the highest in kidney and the dietary Ca and Mg levels were not effected on the copper content of liver. The iron concentration of liver in the Ca-deficient group tended to be greatly higher than that of the other groups. The copper contents of kidney in the Mg-deficient group was lower than that of the other groups, and the iron contents in the deficient groups(II, III, IV) lower as compared with the control group. The copper content of the femur tended to be lower at the deficient levels(II, III, IV) and the copper concentration at the Mg-deficient level was greatly increased as compared with that of the control group.

대혈관 전위증의 병리

조수연,오미혜,김정선,이용희,강행지,서정욱 한국소아심장연구회 2003 소아심장 Vol.7 No.2

초등학교 특수학급 교사의 특수교육보조원 활용실태

정정화,조인수 大邱大學校 師範大學 附設 敎育硏究所 2009 學校敎育硏究 Vol.5 No.1

본 연구는 초등학교에 근무하는 특수학급 교사들의 특수교육 보조원 활용실태를 알아보는데 연구의 주안점을 두었다. 구체적인 연구목적은 첫째, 특수 교육 보조원 활용성과에 관한 초등학교 특수학급 교사의 인식수준을 밝히고 둘째, 초등학교 특수학급 교사들의 특수교육 보조원 활용실태를 밝히는데 있다. 얻어진 결론은 초등학교 특수학급 교사들은 특수교육보조원 제도 운영에 긍정적인 인식을 갖고 있고, 통합교육 교육을 위한 바람직한 제도이며, 학생 및 학부모 모두에게 긍정적인 효과를 내며 특수교육에 질적인 변화를 가져온다고 인식하고 있다. 활용실태 면에서 수업협의, 통합 학급 교사들과의 갈등해소, 팀 체제의 구성, 특수교육보조원에 대한 책임한계, 자질 및 역할 수행에 대한 만족도 차이와 직무연수, 선발ㆍ배치 관리에 있어 학교의 자율성, 학생 인원수에 따른 보조원수 조정, 행ㆍ재정적 지원대책 등을 분석하였다. 구체적인 인식과 실태분석에서 제시된 내용들은 특수교육 보조원의 효율적 활용방안에 기초자료를 제공해 줄 수 있을 것이다. The purposes of this study is to examine the perception of teachers for special classes in elementary schools on the outcomes of the utilization of paraprofessionals for students with disabilities, the actual use of those paraprofessionals by teachers for the use of paraprofessionals in a bid to suggest some reform measures about how to take advantage of paraprofessionals. The subjects of this study were teachers who are in charge of special classes in 60 elementary schools in Daegu. The findings of the study are as follows; First, the elementary school teachers for special classes took a positive view of the paraprofessional system. They thought that the employment of paraprofessionals contributed to laying the solid groundwork for inclusive education, and that educational services provided by them brought a change to the quality of special education. Second, after paraprofessionals started to work, students with disabilities had more opportunities to receive inclusive education as they helped them when they got around or needed another help. The teachers had a discussion with them in advance about instruction, which served to offer more practical inclusive education. To let the paraprofessional system take root, however, there should be mutual confidence between the teachers and paraprofessionals, and management and evaluation criteria should be clarified.

Renca 마우스 종양모델에서 지구성운동이 종양형성에 미치는 영향

조정제,안현종,김성수,김창주 대한스포츠의학회 2003 대한스포츠의학회지 Vol.21 No.1

Subcutaneous injection of cells from renal adenocarcinoma line, renca, into Balb/c mice induces fast growing solid tumors. This method has been used to speculate the efficacy of cytokine treatment for tumor regression. The present study was designed to investigate the effect of treadmill exercise on tumor growth using renca mouse tumor model. We also examined the expression pattern of several genes related to immune response after exercise training for 4 weeks. Exercise training reduced the size of tumor mass compared to that of non-exercised group in tumor cell recipient mice. Natural killer(NK) cell activity showed no significant difference between exercised and non-exercised groups in tumor cell non-recipient mice. Although NK cell activity of non-recipients was higher than tumor cell recipients, NK cell activity of exercised group was significantly lower than non-exercised group among tumor cell recipients. There were no significant differences in mRNA expressions of cytokines. scavenger receptors, and neuropeptieds which are related to immune response, while mRNA expression of TGF-beta was significantly reduced both in spleen and in tumor mass by treadmill exercise. Based on the results presented in this study, endurance exercise has shown the possibility to suppress tumor formation.

1998-1999년 절기에 부산지역에서 유행한 인플루엔자 바이러스의 분리

정영기,정명주,이주연,안정배,김지희,김만수,조경순 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

Investigate the epidemics for influenza outbreaks. The outbreak pattern of the internal and external patients housed in the 10 designated hospitals was monitered to investigated and the characteristics of the virus isolates are as follows. 232 strains of influenza virus was isolated from the oral specimen of 1,320 respiratory disease patients in Pusan from Oct. 1998 to Jun. 1999. Among these isolates, 222 strains were A-type and the rest were B-type. The outbreak pattern for sex-and age-group is as follows. The male outbreak was similar to the female outbreak: male outbreak, 47.4% and female outbreak, 52.5%. Most of the patients were less than 10 years old. The monthly influenza outbreak was consistent from Dec. 1998 to Apr. 1999. and The 113 strains from the A-type isolates were A/Sydney/05/97(H3N2)-like, the 109 strains were A/Beijing/262/95(H1N1)-like, and all of the 10 B-type isolates were B/Harbin/07/94-like.

SOL-GEL법을 이용한 강유전체 BaTiO₃박막 및 Ceramics 제조

조채룡,이수재,장민수,김호종,정세영,노동택,김성철 동의대학교 기초과학연구소 1993 基礎科學硏究論文集 Vol.3 No.1

Concentrated solution was prepared for a sol-gel process of BaTiO₃. Barium hydroxide and Ti-isopropoxide were used as the raw materials with methanol and isopropanol as solvents. Dense ferroelectric polycrystalline BaTiO₃thin films with the perovskite structure were fabricated by spin coating of the prepared concentrated solution on a few substrates. From XRD investigation, it was confirmed that tetragonal phase of polycrystalline BaTiO₃powders are formed after heat treatment above 550℃ in air and grain-oriented BaTiO₃thin films are obtained by coating of 0.06M concentrated solution on the substrates. It was observed that surfaces of thin films deposited on the substrates by our methods were formed crack-free and uniformly. The thickness of thin film which depends the concentration of the solution was 400∼900Å for 1 time coating, which was confirmed by SEM analysis. Ceramics and thin films show dielectric constant of 4600 (at 1㎑) and capacitance 300∼800㎊ (at 1㎑) at room temperature, respectively.