백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

Prednislolne이 골조직에 미치는 영향에 관한 형태학적 연구

송혜정,김무강,김원식,이영호 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.1

To study the effects of corticosteroids to the bone tissues, author grouped young(3 weeks affter birth) and adult ICR mouse by dosage, injected intramusculaily prednisolone, a series of corticosteroids, to posterior leg during 6 weeks per day, obtained femurs and tibias, measured the lengths of tibias and femurs. The portions of the distal end of tibial crests were cross-sectioned, general H-E stain was perpormed. The adjacent distal portions of femoral neck were sectioned longitudinally, observed endosteal surface by scanning electron micrscope The results were followings: 1. The adult group injected by large dosage (0.56mg/100g body weight/day) and the young group injected by small dosage(0.14mg/100g body weight/day) from 3 weeks after birth were significantly shorter than the normal group according to statistics. And the adult group injected by small dosage (0.14mg/100g body weight/day) was not different from normal group according to statistics. 2. In the light microscopic sutdy, the adult group injected by large dosage decreased in bone mass of compact bone severely, the adult group injected by small dosage changed a little grade. The young group injected by small dosage from 3 weeks after birth was not severe in bone change, but bone thickness decreased. 3. In the scanning elctron microscopic study, the adult group injected by large dosage and the young group iniected by small dosage from 3 weeks after birth decreased in trabeculae. The trabeculae of the adult group injected by small dosage were decreased slightly. 4. Integrating above all, the changes by prednisolone injection osteoporosis, of young period were more severe than those of adult, too.

Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

b値 算出과 그 差異 解析에 관한 硏究

宋武永 忠南大學校 環境問題硏究所 1985 環境硏究 Vol.3 No.1

For the regression analysis such as b value in the Gutenberg- Richer relation, Least Square Estimation and Maximum Likelihood Estimation were mathematically reviewed. From the NOAA-EDIS earthquake data file, we calculated the b value in the adjacent to the Korean Peninsula as 0.723 and the East Sea side shows lower value than the Northwest side. The b value of the microearthquakes in the Southeast region of the Korean Peninsula was reviewed. In most cases, there are the b value differences between LSE and MLE for the same data and the differences have no relation with the correlation coefficients and the earthquake total frequencies. The difference will be due to the different assumption for the object data as the Gaussian distribution. Therefore the b value calculation needs everytime the definition of the estimation methods for the b value comparison in space and time.

大淸湖 周邊의 地球科學的 環境硏究

宋武永,朴鍾五 忠南大學校 環境問題硏究所 1989 環境硏究 Vol.7 No.-

The drainage pattern of the Keum River shows a dendritic type in the upperstream composed of granite, and a meandering type in the middle part of metasediments of Okchon system in geoloy. In the expansion of the drainage basin on the basin boundaries, the Keum River is stronger than the Somjin River and the Naktong River, but weaker than the South Han River. The inflow mass quantity is completely dependent on the precipitation, and the average precipitation density shows the maximum 40mm/d. in. August, the minimum less than 0.5mm/d.in January to March, and the medium 2mm/d. more or less in Spring and Autumn. The outflow is composed of the runoff due to the flood control in summer, the discrete industrial water supply, the continuous electricity generation (50㎥/s), and the urban water supply (5㎥/s) Therefore the water elevation in Taechong Lake is dependent on the choice of the flood control time and the industrial water supply to the lower stream. From the observation of the buoy displacement in the lake, it was observed that the water mass was moved with the average velocity 1.4m/s, but with the direction according to time, location, and depth.

忠北 陰城郡 金峰鑛山 試錐 코아의 基本物性과 彈性波 速度와의 關係

송무영,노영길 忠南大學校環境問題硏究所 1993 環境硏究 Vol.11 No.-

음성군의 금봉광산에서 시추한 화강암의 코아시료를 대상으로 기본물성과 P파, S파 속도를 측정하고 그들 상호간의 관계를 탐구하였다. 밀도는 2.63∼2.72g/㎤, 공극률은 0.29∼1.84%, 함수율은 0.09∼0.41%의 범위에서 변한다. P파 속도와 S파 속도는 밀도에 대하여 각각 0.8 및 0.7의 상관도를 갖고 비레한다. 반면 공극률에 대하여는 Vp와 Vs가 각각 0.6 및 0.52의 상관도를 갖고 반비례하는 경향을 보인다. 한편 Vp와 Vs는 높은 상관도(0.8)를 갖고 비례관계를 유지한다. On the granitic core samples of Kumbong Mine in Umsong-Kun, the basic physical properties and the velocities of P and S wave are measured, and their inter-relationship was investigated. The physical properties vary in the of 2.63∼2.72g/㎤ for density, 0.29∼1.84% for porosity, and 0.09∼0.41% for moisture content ratio. P wave and S wave velocities show the proportional tendency with the density within the correlation coefficient 0.8 and 0.7, respectively. On the other hand, about the porosity, Vp and Vs show the inverse proportional tendency within the correlation coefficient 0.6 and 0.52, respectively. Vp and Vs are each other in the proportionate relation within 0.8 of correlation coefficient.

Mongolian gerbil 심장동맥의 해부학적 연구

송치원,정주영,이미영,김무강,현병화 충남대학교 수의과대학 동물의과학연구소 1998 動物醫科學硏究誌 Vol.6 No.-

The arterial supply to heart of mongolian gerbil has been described. Observations were made by the specimens injected with latex after the flowing through in left ventricle. The results were summaried as follows; 1. A. coronaria arose from the initial portion of Aorta in heart and A. coronaria dextra and sinistus arose in Aortic sinus on 10 specimens. A. coronaria sinistra arose more near boundary of Aortic sinus than A. coronaria dextra. 2. A. coronaria sinistra which arose from Aortic sinus divided into Ramus interventricularis sinistra and Ramus circumflexus sinistra. A. coronaria dextra which arose from Aortic sinus only runed continously as Ramus interventriculares dextra. 3. Ramus interventricular sinistra not reach the heart apex through the sulcus interventricula but stopped in thoracocostal plan extending to under part. 4. R. circumflexus runed theough sulcus coronarius, thereafter it runed from left ventricle to Sulcus interventricularis dorsalis. 5. A. coronaria dextra runed to diaphargm side through sulcus interventricula dorsi thereafter it became Ramus interventricular dextra. It distrubuted left and right ventricle wall on 8 specimens and 2 specimen stopped margo obtusus.

도축돈 장분변으로부터 Shiga Toxin-Producing Escherichia coli의 분리와 성상

송영환,김지영,채미경,박창식,김명철,전무형 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

Shiga toxin-producing Escherichia coli (STEC) causes various clinical signs in human and animals, and has been indicated as a global enteropathogen with zoonotic importance. In this study, the feces of healthy pigs were collected from the slaughtered pigs of Daejon abattoir during the period from December 2001 to October 2002. Of 326 specimens, 13 STEC were confirmed by culture, PCR and colony hybridization. The isolates were further studied for toxin types, pathogenic factors, plasmid profiles, and antimicrobial resistance to characterize the genetic and toxigenic properties. In PCR, all of 13 isolates were evident to have shiga toxin gene (stx). Of 13 isolates stx1 gene was detected in 4 and stx2 gene in 9. The genes of eaeA, hlyA and rfbE were not present in any isolates. In colony hybridization using shiga toxin common primer (STXc), 2 to 9 per 100 colonies subcultured from 13 isolates showed the positive reaction. In the examination for plasmid profiles of the isolates, one to eleven plasmids with varying sizes of 1.0 Kb to 100 Kb were detected, and the 13 STEC could be classified into four groups by the plasmid patterns. The antimicrobial resistance patterns of the isolates were comparably corresponded with the plasmid profile patterns.

地形, 水系, 水質 및 地質

宋武永,朴鍾五 忠南大學校 環境問題硏究所 1991 環境硏究 Vol.9 No.-

韓半島 中部圈 地殼物質의 構造와 物性硏究(1) : 淺水灣 周邊의 地質 Geology in the Vicinity of Choˇnsu Bay

宋武永,禹榮均 충남대학교 기초과학연구소 1992 연구논문집 Vol.12 No.-

淺水灣 북부지역 양측 연안에서 이제까지 보고되지 않은 새로운 퇴적층이 발견되었다. 이 퇴적암은 선캠브리아기의 低變成堆積岩(泰安層)과 쥬라기 黑雲母花崗岩으로 구성된 基盤岩을 不整合으로 덮고 있으며 천수만의 윤곽을 나타내므로 淺水灣層으로 명명할 것을 제안한다. 본 천수만층은 역암, 응회암, 현무암, 응회암질 각력암, 셰일로 구성되어 있으며 천수만의 북서부와 북동부의 연안과 중앙부인 죽도 등지에 걸쳐 분포한다. 생성시기는 중생대 말기로 추정된다. 전체 분포 양상이 이곳에 발달한 남북방향의 구조선과 일치하며 여기에 따른 단층작용, 및 화산작용과 밀접한 관련을 갖고 퇴적된 결과로 해석되었다. 이들의 퇴적은 서측에서 동으로 이동하면서 이루어졌다. A previously unreported sedimentary complex has recently been discovered in the northern part of Choˇnsu Bay. It overlies both unconformably the Precambrian low-grade metamorphosed sedimentary sequence(Taean Formation) and the Jurassic biotite granite. The sedimentary complex is proposed to be designated as Choˇnsuman Formation. The Formation is composed of conglomerate, tuff, basalt, tuffbreccia, and shale. It is distributed in the northwest and northeast coasts of Choˇnsu Bay and in the Chukdo. Age of the Formation seems to be of late Mesozoic. The Choˇnsu sedimatary basin is considered to be originated by block faulting presumably during the Cretaceous, as suggested by the development of N-S trending structural lineation in the study area and its vicinity. The Choˇnsuman Formation seems to be formed by sedimantation in close association with block faulting and volcanic activities nearby. Sedimentation is thought to be developed from west to east in the basin.