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감마선분광분석 및 열형광선량측정법에 의한 자연환경방사선의 선량해석에 관한 연구를 1984년 10월부터 약 1년간에 걸쳐 충남대학교 대덕캠퍼스내의 1만㎡ 규모의 평면개활지에서 수행하였다. 이 연구에서 사용한 검출기는 3″ø×3″NaI(TI) 閃光計數器와 plastic에 밀봉하여 금속판에 압착 처리한 chip과 Teflon-disk 로된 2종의 LiF TLD였다. 실측실험으로는 3회에 걸친 24시간 cycle의 in-situ spectrometry와 2회의 3개월 cycle과 1회의 1개월 cycle에 걸친 TLD field dosimetry를 수행하였다. 측정한 모든 spectrum은 G(E)연산자법에 의하여 照射線量率로 환산하였고 그 결과로부터 환경방사선의 地殼成分을 산출하였다. 結果에 의하면 spectrometry로 구한 조사선량율이 평균(10.54±2.96)μR/hr, TLD chip으로 측정해석한 값은 (12.0±3.4)μR/hr, disk에서 얻은 값이 (11.0±3.6)μR/hr로 오차범위 안에서 매우 좋은 일치를 보이고 있다. 그러나 감마선분광분석에 의한 자연방사선의 日變化에는 가끔 심한동요가 관측되었다. 정확한 환경방사선량해석을 위하여 감마선분광분석과 TLD의 적절한 동시 배합사용이 바람직 하며, 보다 고감도의 TLD에 관한 연구와 국제비교등을 통한 선량평가의 精度向上을 위한 보다 깊이있는 연구가 필요하다는 結論에 도달하였다. A study for the assessment of natural environmental radiation exposure at a flat and open field of about 10,000㎡ in area in CNU Daeduk campus has been carried out by means of gamma-ray scintillation spectrometry and thermoluminescence dosimetry for one year period of time from October 1984. The detectors used were 3″ø×3″NaI(TI) and two different types of LiF TLD, namely, chip sealed in plastic sheet which tightly pressed on two open holes of a metal plate and Teflon disk. Three 24-hour cycles of in-situ spectrometry, and two 3-month and one 1-month cycles of field TL dosimetry were performed. All the spectra measured were converted into exposure rate by means of G(E) opertaion, and therefrom exposure rate due to terrestrial component of environmental radiation was figured out. Exposure rate determined by the spectrometry was, on average, (10.54±2.96)μR/hr, and the rates of (12.0±3.4)μR/hr and (11.0±3.6)μR/hr were obtained from chip and disk TLD, respectively. Fluctuations in diurnal variation of the exposure rate measured by the spectrometry were noticeable sometime even in a single cycle of 24 hours. It is concluded that appropriately combined use of TLD with iu-sitn gamma-ray spectrometry system can give more accurate and precise measure of environmental radiation exposure, and further study for more adequate and sensitive TLD for environmental dosimetry, including improvement and elevation of accuracy in data assessment through inter-laboratory or international intercomparison is necessary.
As we were interested in seeing that monitoring of DNA susceptibility to DNase 1 can be a probe of DNA structural transition, we investigated ethidium dependent DNA susceptibility to DNase 1 in the presence (3×10^(-4)M) and absence of spermine. The present study shows different binding features and effect of ethidium bromide on the DNA susceptibility to DNase 1 between the cases of presence and absence of spermine. These data may be useful for the probing structural transition of DNA induced by spermine.
In the 1998 academic year, 33 college junior in the department of statistics who are taking the course of sample theory are divided into 7 groups. And each group conducts a survey which contains all the process of sampling design, such as typing text, customizing color, using icons and clip art and even adding their favorite link The result is an attractive colorful website. [http://stat.chungnam.ac.kr/~sypark]
To identify and characterize Ly-6A.2 ligand, a chimeric protein of Ly-6A.2 and human IgG C_(r)1 was prepared by transfection with the Ly-GA.2/human IgG chimeric cDNA expression vector into mouse SP_(2)/0 myeloma cells. The purified chimeric protein from a positive clone was used to investigate the expression of Ly-6A.2 ligand on cells from lymphoid tissues. The Ly-6A.2 ligand was expressed on subpopulations of bone marrow, thymus, lymph node, and spleen. For the pursuit of the function of Ly-6A.2 ligand, the spleen cells from Balb/c mouse were splitted into the Ly-6A.2 ligand positive and negative cells. In response to allogeneic antigen in vitro, the Ly-6A.2 ligand negative cells showed much stronger proliferative response than the Ly-6A.2 ligand positive cells. These results suggest that the interaction of Ly-6A.2 and its ligand generates a negative signal for cell proliferation. We propose that the Ly-6A.2 ligand positive cells receive an inhibitory signal from Ly-6A.2 antigen by cell-cell interaction.
We prepared two oligonucleotides having Same base pairing but different base sequence in the middle region, d(CGAATTCG) and d(CGTATACG). NMR and UV data showed that such variation in base sequence caused significant differences in thermal stability and dynamics, which is regarded to be associated with the base-sequence specificity on binding interaction between DNA and ligands.
The inhibition pattern of three inhibitors(tacrine, decamethonium and propidium) on the hydrolysis of various thiocholine ester substrates by eel acetylcholinesterase was comparatively examined. When the substrate was acetylthiocholine, it showed a similar competitive inhibition by tacrine inhibitor, and a mixed type inhibition by decamethonium and propidium inhibitors. When the substrate was pentanoylthiocholine, it showed an uncompetitive inhibition by tacrine, and a noncompetitive inhibition by decamethonium. When the substrate was laurylthiocholine, it showed mixed type and uncompetitive inhibition by tacrine, and a competitive inhibition by decamethonium and propidium. Those results suggest that the active site of acetylcholinesterase has the existence of hydrophobic site besides the anionic and esteratic site.
The Government e-Procurement System(GePS) is a comprehensive system to be established for public procurement administration usage, which permits the administration to be more effective in its work. GePS enables all public organizations to conduct the procurement process electronically, and thus makes the procurement administration much more effective and transparent. A single window containing all procurement information allows enterprises a great of convenience in their commerce activities. Additionally, the development of an electronic network means that all information related to the procurement business may be jointly used. This joint usage of information and easy document exchange between public organization and private companies reduces remarkably the amount of paperwork and documentation needed for a contract. The purpose of this paper is to find a way of expanding the usage of GePS by analyzing statistics prepared in early in its operations. The plans of political development for the Procurement administration as the operator of GePS in the time of government digitalization are presented in this paper.
The purpose of product liability law is defined as liable duty for compensation those who damaged or hurted by the defective product, and the law is adopted more than 30 countries in the world. In our country, product liability law was established after examining thoroughly at 1999 and was in forced since 2002. A 'product' is defined as 'all the movable property which is produced or processed' in the Product Liability Law article. 2 clause. 1 and 'electric power' is also applicable to 'product' by means of above article. In this paper, Kepco(Korea Electric Power Corporation)'s reactive strategies in electric power to the enforcement of the Product Liability Law are examined and analyzed.
Recently, contradictions have been raised against the current view that it is advantageous for the enzyme to minimize its interaction with substrate. For the purpose to resolve the contradictions, we examined existing kinetic data of enzymes. The trends of the plots of k_(cat) vs. K_(m) for the catalysis of the enzymes indicate that strong substrate-binding, as reflected in decreased K_(m), is correlated with an increased k_(cat) (i.e.. enhanced chemical conversion of the substrate to product) for the reaction. Thus, the present study shows that the essence of the current view of enzyme catalysis should be reexamined on the basis of the stipulation that enzymes go through conformational change which shift the active site complementarity from substrate-state-specific to transition state-specific.
This review describes the tautomerism and reactivity of 5-membered analogues of cytosine: 5-amino-2H-1,2,4-thiadiazolin-3-one (1). 5-amino-2H-1.2,4-5-amino-2H-1.2,4-oxadiazolin-3-one (2), 3-amino-4H-1,2,4-oxadiazolin-5-one (3) and 5-amino-3H-l,3,4-thiadiazolin-2-one (4).