Algal bioassay 에 의한 조류생장 제한영양염류 결정

오희목(Hee Mock Oh),이석준(Seog June Lee),김성빈(Seong Bin Kim),박미경(Mi Kyung Park),윤병대(Byung Dae Yoon),김도한(Do Han Kim) 한국하천호수학회 1998 생태와 환경 Vol.31 No.2

Determination of Limiting Nutrient for Algal Growth by Algal Bioassay. Oh, Hee-Mock, Seog June Lee, Seong-Bin Kim, Mi- Kyung Park, Byung-Dae Yoon and Do-Han Kim (Environmental Microbiology Research Unit, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, Korea and Water Resources Research Institute, Korea Water Resources Corporation, Taejon 305-390, Korea) Water quaiity and algal growth potential (AGP) using Microcystis aeruginosa were investigated from April to September 1997 in Daechung Reservoir. Secchi disk transparency was 1.1 m in August. Chlorophyll-a concentrations were higher than 10㎍/l at all samples. Total phosphorus concentration was in a range of 0.020∼0.027mg/l which is at the approximate value of 0.03 mg/l, the general critical level of eutrophication. These results indicate that Daechung Reservoir is in an eutrophic state except some temporary times and restricted sites. Phosphorus uptake rate of M. aeruginosa was higher in the water from the epilimnion than in the hypolimnion and also higher under light condition than under dark condition. Therefore, phosphorus uptake rate appears to depend on cellular phosphorous content and light condition, a meaning of endergonic process. Phosphorus was the most frequently limiting nutrient: phosphorus was limiting algal gowth in April and May, nitrogen, phosphorus and trace elements were the limiting factors for algae in August, and trace elements in September. If a fast growing alga, M, aeruginosa, is used as a test organism for assaying AGP, in vivo fluorescence instead of dry weight can be measured for algal biomass and incubation period can be reduced from traditional 14 days to 7 days.

Sandblasted large grit, acid etched 표면처리에 따른 교정용 미니 임플랜트의 제거회전력에 관한 연구

오남희,김성훈,국윤아,이근혜,강윤구,모성서 대한치과교정학회 2006 대한치과교정학회지 Vol.36 No.5

Sandblasted large grit, acid etched(SLA) 표면처리 된 교정용 미니 임플랜트와 평활면을 가진 교정용 미니 임플랜트 사이에 제거회전력과 조직학적 소견을 통해 표면처리된 교정용 임플랜트의 임상적 가능성에 대하여 알아보고자 하였다. 실험재료로는 길이가 9.5 mm, 외경이 1.8 mm인 custom made, screw shaped, titanium implants가 사용되었다. 미니 임플랜트는 두개의 군으로 분류되었는데 SLA군은 20개의 SLA 표면처리된 미니 임플랜트이었고, 평활면군은 크기와 형태가 같지만 SLA처리공정이 생략되어 제작된 20개의 미니 임플랜트로 구성되었다. 이들은 10마리 가토의 경골에 식립되었다. 각각의 가토의 우측 경골에는 SLA군의 미니 임플랜트 2개가 식립되었고, 좌측 경골에는 평활면군의 미니 임플랜트 2개가 식립되었다. 각 군에는 식립 직후 Ni-Ti coil spring에 의해 약 150 g의 지속적인 견인력이 주어졌다. 식립 6주 후에 10마리의 가토를 희생하였고, 안정된 상태에서 Ni-Ti coil spring을 제거하였으며 digital torque gauge를 이용하여 제거 회전력을 측정하였다. 식립 6주 후에 SLA군의 경우 (8.29 Ncm) 평활면군 (3.34 Ncm)에 비해 더 높은 평균 제거회전력을 나타내었고 조직학적 소견에서도 screw 하방에서의 신생골 형성이 관찰되었다. SLA 표면처리된 미니 임플랜트는 평활면 미니 임플랜트에 비해 좀 더 강한 교정력에 저항할 수 있을 것으로 예상되었다. Objective: The purposes of this study were to evaluate the differences between sand blasted, large grit and acid-etched (SLA) treated mini-implants and smooth surface orthodontic mini-implants in relation to the removal torque as well as the histologic analysis. Methods: Custom-made, screw-shaped, titanium implants with a length of 9.5 mm and an outer diameter of 1.8 mm were divided into 2 groups; the SLA group (20 SLA treated orthodontic mini-implants) and the smooth surface group (20 smooth surface mini-implants), and placed in the tibia metaphysis of 10 rabbits. Each rabbit had 4 mini-implants placed, 2 in each tibia. The right tibia were implanted with the SLA group mini-implants and the left tibia had the smooth group mini-implants placed. Each mini-implant group were immediately applied with a continuous traction force of 150 g using a Ni-Ti coil spring. The rabbits were sacrificed 6 weeks post-surgically. Subsequently, the legs were stabilized, the Ni-Ti coil springs were removed and the mini-implants were removed under reverse torque rotation with a digital torque gauge. Results: 6 weeks after placement, the SLA group presented a higher mean removal torque value (8.29 Ncm) than the smooth group (3.34 Ncm) and histologic analysis revealed a higher new bone formation aspect along the screw in the SLA group. Conclusion: Results of this study indicates that SLA treated mini-implants may endure higher orthodontic forces without loosening.

Neisseria gonorrhoeae 분리균의 항생제 내성 양상 분석

성원근,정경태,길준영,김석하,오희복 대한감염학회 2001 감염 Vol.33 No.5

Background : The aim of this study was to monitor trends in antimicrobial susceptibilities of Nesseria gonorrhoeae isolates, in particular, to examine the possibility of increasing prevalence of fluoroquinolone resistance in Korea and the relationship between patterns of mutations involving gyrA and parC genes and the ciprofloxacin resistance level. Methods : The antimicrobial susceptibilities of 489 gonoccocal isolates which were nationwide collected from patients visiting Health Centers were determined by NCCLS disk diffusion and agar plate dilution methods. PCR and direct DNA sequencing of the amplicons were performed to identify mutations within the quinolone resistance-determining regions of gyrA and parC genes. Results : The proportion of resistance to penicillin, tetracycline, or both remained as high as 94%. The isolates with ciprofloxacin resistance remarkably increased from 1% in 1999 to 5% in 2000 and also resistance to cefoxitine and ceftriaxone were shown to be increased. The strains resistant to spectinomycin was little reported. Four isolates with 16 ㎍/mL of MIC for ciprofloxacin all showed the same alternations of Ser-91 to Phe, Asp-95 to Gly in GyrA and Ser-87 to Arg in ParC, but ciprofloxacin-susceptible strains have little amino acid substitution. Conclusion : Considering the increasing prevalence of isolates with resistance to ciprofloxacin and ofloxacin, it is likely that the antibiotics such as spectinomycin, or ceftriaxone are recommended as the first-line treatment for gonoccocal infections in Korea. The results from this study suggest that mutation analysis for quinolone resistance-determining regions of gyrA and parC genes are important in epidemiological studies for the spread of ciprofloxacin resistant strains.(Korean J Infect Dis 33:338∼345, 2001)

하악골 골절에 대한 임상통계학적 연구

이성훈,김기영,조규승,소광섭,박홍주,조요기,오희균,유선열 大韓顎顔面成形再建外科學會 1998 Maxillofacial Plastic Reconstructive Surgery Vol.20 No.1

인터넷 배너광고의 유형에 따른 광고 효과

오지은,김성희,이상빈 전북대학교 사회과학연구소 2003 지역과 세계 Vol.29 No.-

This study was to investigate the effect of internet banner types(texts, animations, sexy models) on indexes of advertising effects(attitude toward the advertisement( A_ad), brand attitude( A_b), purchase intention(PI)]. In this study, 180 subjects were conveniently sampled, and randomly assigned to one of 6 conditions in 3(ad types) × 2(products types) factorial design. The major findings of this study as follows. The results of A_ad on differences compared with the ad using texts and sexy models. And the ad for computers had statistically significant differences from ad for books. The results of A_b on ad types had significant main effects, but the results of A_b on products types and interaction effects had no significant differences. The ad using texts and animations had statistically significant differences from the ad using sexy models. The results of PI on ad types had significant main effects, but the results of PI on products types and interaction effects had no significant differences. The ad using texts and animations had statistically significant differences from the ad using sexy models. Finally, implications and limitations of this study were discussed.

在來市場 再整備 戰略의 方向 : 미국·일본의 소매시장 활성화 전략을 중심으로

한희경,김정태,오덕성 忠南大學校 地域開發硏究所 1997 地域開發論叢 Vol.9 No.-

The purpose of this is to present effective strategy of rehabilitations, renewals on traditional retail markets which have been pressed from emerging of new types of retail shops, increased income, changing of consumer's merchandising pattern. For this study, the cases of America, Japan were chosen and analyzed according to effective ways of rehabilitations, renewals on traditional retail markets. The results were as follows. ① The strategy in Japan rehabilitations depending on tenant's participation present architectural guidelines our situation consisting of petty market and organization. ② The strategy in America give functional mix, tenant mix, strategical market, markets with regional character in markets renewal which were applied to out situation associated with clearance.

Lesch-Nyhan 증후군 환아의 자해에 의한 혀의 외상

김지희,최병재,김성오,최형준,손흥규,이제호 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.3

Lesch-Nyhan 증후군은 드물게 나타나는 X 염색체 열성 유전 질환으로 purine의 대사 장애를 보이는 질환이다. Purine의 대사 장애는 대사에 관여하는 효소인 hypoxanthine guanine phosphoribosyl transferase의 결손 또는 활성 감소로 인하여 나타나며,요산의 과다 생산에 의한 요산과다혈증,요산과다뇨증 등의 신장계 증상과 정신지체,무도증 등의 신경학적 장애를 보이고,구강이나 손가락에 대한 자해를 나타낸다. 이 중 자해 행위는 입술과 혀,손가락을 깨무는 경우가 흔하고,심하면 혀와 손가락이 완전히 절단될 수도 있다. 이 경우 자해로 인한 통증뿐 아니라,연조직 결손으로 인한 심미적인 문제와 상처 부위로의 2차 감염이 발생할 수 있다. 이러한 Lesch-Nyhan 증후군 환자에서 자해를 막기 위해 여러 방법이 사용되고 있는데,이 중 치과적으로 접근하는 방법으로는 마우스 가드나 lip bumper 등의 장치를 이용하는 방법과 해당 치아들을 모두 발거하는 방법,악교정 수술을 통해 개방 교합을 형성해 주는 방법 등이 있다. 본 증례는 13세 9개월의 Lesch-Nyhan 증후군 환아로서 자해에 의해 혀에 심각한 외상을 가한 경우로 마우스 가드를 이용한 보존적인 방법을 사용하였으나 계속적으로 혀에 외상을 가해 하악 전치 뿐 아니라 구치까지 발거하여 치료하였다. Lesch-Nyhan syndrome is a rare X-linked recessively inherited disorder, caused by complete absence or decrease in activity of hypoxanthine guanine phosphoribosyl transferase(HPRT), an enzyme involved in purine metabolism. This enzyme deficiency gives rise to nephropathy symptoms, such as hyperuricosuria and hyperuricemia by excessive uric acid production and neuropathy symptoms, such as mental retardation, choreoathetosis and self mutilation behavior. Patients with Lesch-Nyhan syndrome have tendency to bite their liP, tongue and finger, In severe cases, partial or even total amputation of tongue or finger occur. Self-inflicted bites are often complicated by secondary infection to the injured site as well as pain. Furthermore tissue loss by biting results in esthetic problems. The dental management of self mutilation includes treatment with appliances such as soft mouth guard or lip bumper, extraction of all the teeth, and orthognathic surgery. We report a case of a 13 year-old boy with Lesch-Nyhan syndrome, who severely injured himself on his tongue. At first, conservative treatment using soft mouth guard was considered, but it could not prevent trauma on his tongue. Therefore, extraction of the lower anterior and posterior teeth was carried out.

말기심부전 환자의 부전심장에서의 장바이러스와 인형 거대세포바이러스 감염양상

최성준,김석연,홍석근,김효수,오병희 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.9

IMF에 관련된 경제적 스트레스가 정신 건강에 미치는 영향

유희정,황성훈,홍진표,김헌수,이철,한오수 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.1

본 연구는 IMF에 관련된 경제적 스트레스가 정신 건강에 미치는 영향과 그 과정을 밝히기 위해 설계되었다. 스트레스 과정에 관여하는 요인으로 사회적 지지와 문제 해결 양식, 그리고 무망감을 도입했으며, 이들에 의해 스트레스의 영향이 매개되는지를 검증하였다. 경제적 스트레스의 수준에 따라 표집된 221명의 일반인들을 대상으로 우울, 불안, 신체화, 적대감, 사회적 지지, 그리고 문제 해결 양식 등을 측정하였다. 가정한 대로 실직으로 대표되는 경제적 스트레스는 정신 건강에 부정적 영향을 미치는 것으로 나타났다. 그 구체적인 양상을 살펴보면 다음과 같다. 먼저, 사회적 지지는, 높은 스트레스 조건에서 증상의 발현을 억제하는 완충 효과가 있음이 입증되었다. 두번째로, 남성이 여성에 비해 경제적 스트레스의 부정적 영향에 더 취약한 것으로 나타났다. 세번째, 경제적 스트레스는 문제 해결 양식의 다양한 구성요소중 문제 해결에 대한 무기력감에만 영향을 미치는 것으로 나타났다. 네번째, 무망감은 경제적 스트레스와 정신 건강간의 관계를 매개하는 변인임이 인과모형 검증을 통해 확인되었다. 끝으로, 이러한 결과가 스트레스 개입 프로그램 개발을 위한 이론적 기초를 제공한다는 의의가 있음을 논의 했고, 본 연구의 몇 가지 한계점을 지적하였다. This study was designed to investigate the influence of the so-called IMF-related economic stress upon the mental health and to analyze the process of the influence. As the possible variables moderating the stress process, social resource, problem solving style, and hopelessness were included. 221 male and female subjects were recruited according to the intensity of economic stress and classified into four groups(employees of solid companies, employees of the companies at risk, short-term unemployed and long-term unemployed). Relevant measures including economic stress scale, selected SCL-90R subscales(somatization, depression, hostility, anxiety), social support scale, problem-solving scale, and job hopelessness scale were administered. As assumed, it was proved that economic stress had adverse effects on the mental health. In addition to this basic causal relationship, moderating effects of several variables were found as follows: First, social support had the buffering effect upon the economic stress. That is, strong social support played the role suppressing the development of psychiatric symptoms under high stress. Second, men were more vulnerable to the adverse effects of economic stresses than women. Third, economic stresses had influence on the helplessness concerning the problem solving. However other problem solving strategies such as creative style, approach style, avoidance style, etc were proven to have no relationship with the economic stress. Fourth, in the analysis of unemployed group, it was found that hopelessness mediates the relationship between economic stress and mental health. In conclusion, the clinical implications of such findings in economic stress process were discussed and several limitations and the suggestions for future researches were also addressed.

페놀분해 효모 Candida tropicalis PW-51의 분리 및 분해특성

김성빈,김치경,김희식,이창호,신기선,권시석,윤병대,오희목 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

본 연구는 페놀과 포름알데히드를 포함하는 페놀계수지 산업폐수의 생물학적 처리에 이용할 목적으로 포름알데히드의 존재하에서 페놀분해능이 우수한 효모를 sludge로부터 분리하여, 형태적 및 생리 생화학적 특징을 조사하여 Candida tropicalis PW-51로 동정하였다. 회분식 배양에서 C. tropicalis PW-51의 페놀 분해한계는 2,000 mg/ℓ이며, 58시간내에 완전히 분해하였다. C. tropicalis PW-51은 초기접종량이 9×10 exp (6) cells/㎖, 배양온도는 30℃, pH는 7.0에서 페놀 분해효율이 높았으며, 500~2,000 mg/ℓ의 페놀농도에서 조사된 페놀에 대한 평균 분해율은 페놀 1,000 mg/ℓ에서 45.5 mg/ℓ/h 가장 높았다. C. tropicalis PW-51의 페놀분해시 catechol 1,2-dioxygenase의 활성이 크게증가하므로 ortho-path-way에 의해 페놀을 분해하는 것으로 판단되었다. 따라서, C. tropicalis PW-51는 페놀계 수지 산업폐수의 생물학적 처리에 효과적으로 이용될 수 있는 균주로 사료된다. For the biological treatment of phenolic resin wastewater containing phenol and formaldehyde, a phenol-degrading yeast was isolated from the papermill sludge, and then identified as Candida tropicalis PW-51 according to morphological, physiological and biochemical properties. The strain was able to degrade high phenol concentrations up to 2,000 mg/ℓ within 58 hours in batch cultures. Phenol-degrading efficiency by the strain was maximum at the culture conditions of a final concentration of 9×10 exp (6) cells/㎖ 30℃ and pH 7.0. The mean degradation rate of phenol was highest at 45.5 mg/ℓ/h in 1,000 mg/ℓ phenol from 500 mg/ℓ to 2,000 mg/ℓ phenol. Because the enzyme activity of catechol 1,2-dioxygenase increased in the course of degradation of phenol, it seems that this strain degrades phenol via the ortho-cleavage of benzene ring. The isolate C. tropicalis PW-51 could be effectively used for the biological treatment of phenolic resin wastewater.