발효조를 이용한 Monascus anka의 적색소와 황색소의 생산

강성국,임종환,정순택,김선재 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

Monascus anka를 이용하여 천연식용색소를 대량생산하기 위한 방법을 개발하기 위하여 발효조를 이용하여 적색소와 황색소의 색소생산의 최적 배양 조건과 생산된 색소의 색깔 특성을 조사하였다. Monascus anka 적색소와 황색소를 생산하였는데, 이들 색소는 균체내색소와 균체외 색소로 구분되었다. 균체의 적색소(ERP)는 494nm에서, 균체외 황색소(EYP)는 380nm에서, 균체내 적색소(IRP)는 506nm에서 그리고 균체내 황색소(IYP)는 388nm에서 최대 흡광도를 나타냈다. 적색소와 황색소 생산, 색조의 특성 및 균체증식력을 배양 온도, pH, 쌀가루 농도, peptone 농도, magnesium sulfate 농도, 통기량 및 교반속도에 대하여 조사한 결과 적색소 생산력은 30℃, 초기 pH 6.0, 쌀가루 농도 3~5%, peptone 농도 0.05%, magnesium sulfate 농도 0.25%, 통기량 0.1vvm 교반속도 300rpm의 조건에서 최대치를 보였으며, 이때 ERP, EYP, IRP 및 IYP는 각각 A_494nm 0.84, A_380 nm 0.71, A_506 nm 1.18, A_388 nm 1.10, L값은 각각 29.40, 6.44, 34,98, 8.94, a값은 각각 5.76, -1.08, 11.83, -1.55, b값은 각각 18-19, 3.41 27.26, 6.16 그리고 균체량은 7.4 g/l을 보였다. 또한 황색소 생산력은 온도의 경우 적색소 생산을 위한 최적온도 보다 높은 35℃ 부근에서 우수하였으며 초기 pH와 쌀가루 농도는 적색소 생산을 위한 최적조건과 같았고, 질소원과 무기염의 농도가 높을수록 그리고 통기량이 많을수록 황색소 생산력이 우수하였다. 적색소와 황색소 생산을 위한 발효조의 교반속도는 100~300 rpm이 적합하였다. Production of Red and Yellow Pigments from Monascus anda in a Jar Fermenter, Seong-Gook Kang, Jong-Whan Rhim, Soon-Teck Jung^* and Sun-Jae Kim. Department of Food Engineering. Mokpo National University Chonnam, 534-729, Korea - In order to develop the method for mass production of natural food colorant from Monascus anka, optimum cultivation conditions for producing red and yellow pigments by cultivating the mold in a jar fermenter and their color characteristics were investigated. The mold produced red and yellow pigments both intracellularly and extracellularly. These pigments showed unique light absorption characteristics with maximum absorption of 494, 380, 506, and 388 nm for extracellular red pigment (ERP), extracellular yellow pigment (EYP), intracellular red pigment (IRP), and intracellular yellow pigment (IYP), respectively. Optimum conditions for producing red pigments were found to be temperature 30℃, initial pH 6.0, rice powder 3~5%, peptone 0.05%, magnesium sulfate 0.25%, aeration rate 0.1vvm. Optimum temperature for producing yellow pigments was around 35℃ which is higher than that of producing red pigments. The initial pH rice powder concentration for producing yellow pigments were the same as those of producing red pigments. The higher concentration of nitrogen source and inorganic salt, aeration rate, the more the yellow pigments were produced. Them optimum agitation speed was 100~300 rpm for pigment production.

1990년대 한국의 직업성암

강성규,안연순,정호근 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.4

The proportion of cancer as a cause of dearth in Korean has been continuously increas-ing. In 2000, 24 % (59,020 of 247,346) of deaths were caused by cancer. Occupational exposure would have contributed to the deevelopment of some cancers. It would have accounted for more than 2,000 cancers in a year if 4% of all cancer or 10% of lung cancer was regarded as arising from the work environment. However, occupational cancer has not been reported as much as expected. The first case of occupational cancer reported officially was mesothelioma caused by asbestos exposure at an asbestos textile industry in 1992. Following the report, many cases of occupational cancers have been reported such as lung cancer due to exposure to asbestos, chromium, exhaust gases, coke oven emissions, and silica, as well as leukemia due to exposure to benzene or other solvent and bladder cancer arising from exposure to benzidine salts. The Health Examination for retirees has been conducted since 1992 in order to detect occupational cancer early in workers who have been exposed to 11 carcinogenic sub-stances. However, to date no occupational cancer has been detected through the Retirees' Health Examination. The Korea Labor Welfare Corporation (KLWC) has referred 108 claims for occupational cancer to the Korea Occupational Safety and Health Agency (KOSHA) between 1992 and 2000. Thirty-three cases were accepted, including 14 cases of lung cancer, eight cases of hematopoietic cancer and six cases of mesothelioma. KLWC has accepted the claim for primary lung cancer developed with pneumoconiosis as an occupational cancer, of which there were 31 cases in 1999 and 61 cases in 2000. In conclusion, special attention is required to detect occupational cancer due to their long latency period and the fact they are usually found after retirement.

HPLC-ECD를 이용한 흰쥐의 뇌 중 감마아미노부티르산 및 글루탐산의 정량

강종성,이순철 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

A sensitive and efficient assay method was applied to determine the level of glutamic acid (GA) and γ-aminobutyric acid (GABA) in frontal cortex and hippocampus of rat administrated with ethanol and drugs. The compounds were derivatized with ο-phthalaldehyde (OPA) and 2-mercaptoethanol for precolumn analysis. The condition for the simultaneous determination of GA, GABA and beta-aminobutyric acid (BABA) by high performance liquid chromatography with electrochemical detection was reverse phase C_18 column as stationary phase, 0.1M phosphate buffer containing 0.1mM Na_4EDTA: methanol=55:45 (v+v) pH 3.8 as mobile phase and 0.7V electrode voltage. The stability of reaction product of GA, GABA and BABA with OPA could be increased by adding the same volume of polyethylene glycol 400 to reaction mixture. The GABA level in frontal cortex of rat was significantly decreased by the administration of picrotoxin and diazepam, but it was significantly increased by the administration of red ginseng total saponin, N-methyl-D-glucamine and (-)-deprenyl.

農業構造改善과 營農規模 擴大方向

姜奉淳,尹昇浩 韓國農業政策學會 1991 농업경영정책연구 Vol.18 No.1

전국 단위 감시체계의 현황과 전망

강성규,안연순,지영구,김형옥,하미나,권호장,백남종,김성아,홍윤철,김재용,강대희,정혜선,하은희 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.2

8년간(1992~1999) 산업안전보건연구원에 의뢰된 직업병 심의 사례 분석

강성규,김규상,김양호,최정근,안연순,진영우,최병순,양정선,김은아,채창호,최용휴,김대성,박정선,정호근 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : Pneumoconisis and noise-induced hearing loss (NIHL) have been reported as main occupational diseases by the Special Health Examination. The Industrial Accident Compensation Insurance has reported various work-related diseases, however, these two diseases occupied almost a half of compensated cases. Therefore, it was not well known about the status of occupational diseases other than pneumoconiosis, NIHL, and cardio-cerebrovascular accident (CVA). This study was conducted to analyze claimed cases as an occupational disease, that was requested to the Korea Industrial Safety and Health Agency (KOSHA). Methods : The local office of the Korea Labor Welfare Corporation (KLWC) has asked the KOSHA for confirmation of claimed cases as an occupational disease. We analyzed 379 cases requested from KLWC, the Ministry of Labor, employers, unions and occupational health agencies from 1992 to 1999. Results : Male was 80.7 % of the requested cases. Their mean age was 42 years old and 75.5 % of them were more than 35 years old. The requested cases were increased rapidly from 25 cases in 1992 to 108 cases in 1999 and the accept rate was 50.7 %. The majority of requested cases were respiratory diseases(22.4%), cancers(18.5%), Neuropsychiatric problems(14.5%), and musculoskeletal problems(13.5%). The accept rate was high in reproductive, respiratory, musculoskeletal and digestive disorders and low in neuropsychiatric, renal and otologic problems and occupational cancers. 73.6% of them were caused by chemical agents, especially 28.5 % were by organic solvents. 67% of them were clinically confirmed at university hospitals. A half of the cases were from KyongIn area, even the request came from the whole country. Conclusions : A claim was common in workers whose age was over 35 years old and exposure history was over 10 years. The respiratory diseases and neuropsychiatric disorders were still main problems in occupational health and occupational cancers was increasing even though its accept rate was not high yet.

HPLC-ECD를 이용한 흰쥐 뇌의 선조체 중 신경전달물질의 동시분석시 최적 조건

강종성,문민선,신형선,이순철 충남대학교 약학대학 의약품개발연구소 1995 藥學論文集 Vol.11 No.-

흰쥐 뇌의 선조체로부터 catecholamine, indoleamine 및 대사산물을 동시분석할 수 있는 간편한 방법을 제시하였다. 기기는 ECD를 장착한 HPCL를 사용하였고, 고정상인 C_18 컬럼에서, 이동상은 10mM citric acid, 0.13mM Na_4EDTA, 0.58mM SOS를 함유하는 35mM sodium acetate buffer(pH 3.4) : MeOH=85:15가 최적이었다. 이 때 컬럼온도는 30℃였다. 이 조건으로 dopamine(DA), 3, 4 -dihydroxyphenyl acetic acid(DOPAC), homovanilic acid(HVA), 5-hydroxyindole acetic acid(5-HIAA), serotonin(5-HT) 및 noradrenaline(NA)을 미량까지 분석할 수 있었고, 검출한계는 각 성분에 대해 1회 주입당 2~10pg이었다. 이 방법으로 흰쥐의 주령 및 성별에 따른 선조체내 생체아민과 대사산물의 함량을 비교하였다. 7주령 쥐에서 암컷은 수컷보다 DA와 5-HT의 함량이 유의성 있게 높은 것으로 나타났으며, 주령이 증가함에 따라 암컷에서 DOPAC의 함량이 유의적으로 증가하였다. A simple, efficient and sensitive method was described for the simultaneous determination of catecholamine, indoleamine and related metabolites from the homogenates of the rat brain striatum by HPLC-ECD. The optimum mobile phase on a reverse phase C_18 column was 35mM sodium acetate buffer(included 10mM citric acid, 0.13mM Na_4EDTA, 0.58mM SOS, pH 3·4):MeOH=85:15. The column temperature was 30℃. Dopamine(DA), 3, 4-dihydroxyphenyl acetic acid(DOPAC), homovanilic acid(HVA), 5-hydroxyindole acetic acid(5-HIAA), serotonin(5-HT) and noradrenaline(NA) could be separated and analysed to very small amount. The detection limits of this method were 2~10pg per injection for all components. The effects of age and sex of rat on the contents of the catecholamines and their metabolites in rat brain striatum were studied. The levels of DA and 5-HT contents of the 7 week old female rats were higher than those of the 7 week old male rats. As the age of rat increases, the contents of DOPAC increased significantly.

홍국균의 최적배양조건에 관한 연구

정순택,임종환,박양균,황금택,박현진,안찬영,강성국,문길만 木浦大學校 工業技術硏究所 1994 工業技術硏究誌 Vol.4 No.-

Cell growth and pigment productivity of Monascus anka KCCM 11832 and Monascus sp. has isolated from the Korean Maeju(soybean koji) were determined for effect of culture medium composition and incubation conditions. Increase of NH₄NO₃, and KH₂PO₄ concentration as mineral source promoted mycelium growth, and the highest cell growth was showed in the medium with 0.05% KH₂PO₄ when C/N ratio is 20:1, but pigment production was significantly inhibited at higher concentration than this level. Cell growth and pigment production in porato dextrose medium were higher than those in czapeck-dox broth and malt extract medium. In terms of temperature, the highest cell growth was showed at 34℃, but pigment productivity was the highest at 26℃.

이온쌍 크로마토그래피를 이용한 혈액 중 Pravastatin의 분석

이순희,임환미,린팜두안,강종성 충남대학교 약학대학 의약품개발연구소 藥學論文集 Vol.16 No.-

A new HPLC method for the determination of pravastatin, a HMG-CoA reductase inhibitor, in human plasma is described. Sample preparation and separation on a reversed-phase column were carried out by the principle of ion-pair formation of pravastatin with alkyl amines as ion-pair reagent. Pravastatin and triamcinolone acetonide (internal standard) were extracted from human plasma with chloroform in the presence of 0.2 mM tetrabutyl ammonium ion and separated on a C_18 column with 30% acetonitrile in phosphate buffer (pH 6, 10 mM) containing 2 mM tetraoctyl ammonium ion. The limit of determination in plasma was 5 ng/ml.

시설재배 작물의 적정 시비량 결정을 위한 간이 검정법

홍순달,임태준,강성수,양원석 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 2002 연구보고서 Vol.6 No.-

토양의 질산태 질소 함량이 67㎎ ㎏-1에서 343 ㎎ ㎏-1의 분포를 갖는 8개 시설재배 토양에서 시비수준을 무비구와 표준시비구로 하여 오이의 생산력을 검정하고 토양 화학성과의 상호관계 및 회귀관계의 평가로부터 질소 적정 시비량 추천모델을 추정하였다. 토양의 질산태 질소 함량과 무비구 오이의 생체중, 비료효과 및 시비효율의 상호관계를 Cate & Nelson 분할법에 의하야 추정한 질소 시비의 상한기준은 280㎎ ㎏-1으로 추정되었다. 토양의 질산태 질소 수준에 따른 표준시비구의 생체중 변화의 관계로부터 토마토 질소 표준시비를 위한 질산태 질소 하한기준은 100㎎ ㎏-1으로 추정되었다. 따라서 상한기준과 하한기준 사이의 질산태 질소 검정에 의한 토마토, 오이, 배추의 질소 시비량 추천모델은 다음과 같이 개발하였다. 질소 표준시비량에 대한 비율 Y=-0.5556X +155.568. 재배기간이 장기간인 오이의 질소 덧거름 시비량 결정방법을 확립하기 위하여 ChlorophyⅡ meter (SPAD502, Minolta)를 활용한 엽록소 검정법을 검토하였다. 평가방법은 NO3-N 함량이 67∼343㎎ ㎏-1인 8개 토양에서 무비구 오이의 생체중과 경시적 엽위별 엽록소 측정치와의 회귀관계로부터 오이의 생산능력과 질소공급능력에 대한 엽록소 측정치의 기준을 추정하였다. 오이 재배기간 동안 엽위별 엽록소 측정치는 정식 후 39일째 13매 잎에서 최대를 보인 후 감소되었으며, 그 이후 오이의 엽록소 측정치는 토양의 질소 공급능력에 따라 현저한 차이를 나타냈다. 정식 후 39일째 13매엽의 엽록소 측정치는 토양의 질산태 질소, 무비구 오이의 생체중 및 질소 흡수량, 시비효율과 유의성 있는 상관관계를 보였다. 그리고 오이 최대 생체중에 대한 부족비율은 13매엽 엽록소 측정치와 유의성 있는 부의 상관을 보였으며 엽록소 최대치에 대한 부족비율과는 곡선적인 정의 상관을 보였다. 따라서 오이 최대 생체중에 대한 부족비율을 덧거름 요구량이라고 가정하면 13매엽의 엽록소 측정치는 덧거름의 사용시기와 기준을 결정할 수 있는 가능성을 보였다. 따라서 시설재배 오이의 밑거름은 질산태 질소의 토양검정에 의하여 결정하고 덧거름은 오이 정식 후 39일째 13매엽의 엽록소 측정으로 결정할 수 있는 종합적인 질소 시비추천 방안을 확립하였다.