시설재배 작물의 적정 시비량 결정을 위한 간이 검정법

홍순달,박효택,김기인,한갑동 충북대학교 첨단원예기술개발연구센터 1999 연구보고서 Vol.4 No.-

시설재배 토양의 질산태 질소 간이검정법을 선발하기 위하여 test strip을 이용한 Nitracheck Reflectometer법과 휴대용 비색계를 이용한 Hanna Ion Specific meter법을 실험실 kjeldahl법 및 이온 전극법과 함께 비교 분석하였다. 평가방법의 신뢰도는 NO3-N 함량이 10 mg/kg에서 340 mg/kg으로 분포되는 20개 시설재배 토양에 대하여 표준편차, 변이계수 및 회수율을 비교하였다. 휴대용 비색계법은 낮은 농도와 높은 농도에서 큰 오차범위를 보였으나 Nitracheck Reflectometer법은 두가지 실험실법과 유사한 신뢰도와 회수율을 보였다. 따라서 간편한 분석조작과 짧은 분석시간을 구비한 Nitracheck Reflectometer법은 신뢰성 있는 간이 검정법으로 활용 가능한 것으로 평가되었다. 시설재배 작물의 질소 덧거름 시용량의 결정을 위한 식물체 간이 검정법(SPAD, Minolta 502)을 개발하기 위하여 엽록소 측정치와 토마토의 생산능력 및 토양의 질소공급능력과의 상호관계를 조사하였다. 평가방법은 NO3-N 함량이 55~306 mg kg-1인 6개 토양에 대하여 무비구 토마토의 건물중과 질소 흡수량, 그리고 재배기간 동안의 경시적 엽위별 엽록소 측정치와의 회귀관계로부터 토마토의 생산능력과 질소공급능력에 대한 엽록소의 측정치의 기준을 추정하였다. 토양의 질산태 질소 함량과 무비구 토마토의 건물중 및 질소 흡수량의 상호관계는 유의성 있는 정상관의 곡선적 관계를 보였으며 최대 건물중과 질소 흡수량에 대한 질산태 질소의 함량은 각각 215 mg kg-1과 208 mg kg-1로 추정되었다. 이는 시설재배 배추의 질소 시비량 결정을 위한 질산태 질소의 상한기준 200 mg kg-1의 평가기준과 유사하였다. 토마토 재배기간 동안 엽위별 엽록소 측정치는 정식후 45일째 15매 잎에서 최대를 보인 후 감소되었으며 정식후 45일째 이후 토마토의 엽록소 측정치는 토양의 질소 공급능력에 따라 현저한 차이를 나타냈다. 토마토 잎의 엽록소 측정치는 단위 엽면적당 질소 흡수량과 유의성 있는 정의 상관을 보여 질소 영양진단을 위한 간이 검정법으로 활용 가능성을 보였다. 정식후 40일째 14매엽의 엽록소 측정치는 토양의 질산태 질소, 무비구 토마토의 건물중 및 질소 흡수량과 유의성 있는 정 상관의 곡선적 관계를 보였다. 부비구 토마토의 최대 건물중과 최대 질소 흡수량에 대한 엽록소 측정치의 기준은 각각 57.1로 동일하게 평가되었다. 무비구 토마토의 최대 건물중과 질소 흡수량으로 평가된 엽록소 측정치 57.1에 대한 토양의 질산태 질소 함량은 약 200 mg kg-1로 추정되어 무비구 토마토의 최대 건물중과 질소 흡수량으로 평가된 질산태 질소 기준 208~215 mg kg-1과 비슷하게 평가되었다. 따라서 엽록소 간이검정법은 토양의 질소공급능력을 예측할 수 있는 유효지표로 활용할 수 있는 것으로 생각되었다. To find a rapid determination method for soil nitrate concentration, two methods, "Nitracheck 404" reflectometer(Eijkelkamp, Netherlands) with nitrate test strip and a portable colorimeter "Hanna Ion Specific meter(USA) with a color development reagent, were tested for twenty soils with different nitrate contents ranged between from 10 mg kg-1 to 340 mg kg-1 and compared Standard deviation, coefficient of variability, and recovery of these values with those measured by conventional Kjeldahl distilation method and nitrate electrode method. The nitrate concentration measured by portable colorimeter method was higher in soils with low concentration and lower in soils with high concentration than that measured by conventional methods. However, concentrations measured by nitracheck reflectometer method was showed the similar coefficient of variability and recovery as those by conventional methods for all soil samples. With this result, the Nitracheck reflectometer method was thought to be available for rapid determination of soil nitrate with accuracy, quickness, and simplicity for operating of analysis. To find diagnosing method of nitrogen status in tomato plant for determining optimum application rate of side dress, chlorophyll content were measured by portable chlorophyll meter(SPAD 501, Minolta), and compared it soil nitrogen status. Regression between dry weight, nitrogen accumulation in tomato grown with no fertilizer and chlorophyll content at different stalk positions of tomato were evaluated for 6 soils with different nitrate concentrations ranged between from 55 mg kg-1 to 306 mg kg-1. Soil nitrate concentration was significantly correlated with dry weight and nitrogen accumulation in tomato grown with no fertilizer. And the critical levels of soil nitrate for maximum dry weight and nitrogen accumulation were 215 mg kg-1 and 208 mg kg-1, respectively. These critical levels of soil nitrate were similar to the estimating level (200 mg kg-1) for Chinese cabbage with no fertilizer under the greenhouse. The chlorophyll content of tomato occurred peak at the 15th leaf of stalk position on the 45 days after transplanting and this suggested that before and near the 45 days after transplanting is the critical time for chlorophyll test of tomato for diagnosing nitrogen status. And the chlorophyll content of tomato grown with no fertilizer after the critical time was affected by the level of soil nitrate concentration, which suggested that chlorophyll content after 45 days after transplanting reflect the soil nitrogen supplying capacity. The chlorophyll content of tomato leave was significantly correlated with amount of nitrogen per unit area of leave. It was suggested that chlorophyll content is useful for nitrogen diagnosis of plant. And the chlorophyll content of the 14th leaf at the 40 days after transplanting was significantly correlated with soil nitrate status, dry weight of tomato and nitrogen accumulation in tomato plant grown with no fertilizer. Chlorophyll content for maximum dry weight and nitrogen accumulation in tomato plant grown with no fertilizer were 57.1 mg kg-1 that represent soil nitrate as 200 mg kg-1. This critical level of soil nitrate was similar to those of 215 mg kg-1 and 208 mg kg-1 estimated by dry weight and nitrogen accumulation in tomato plant grown with no fertilizer, respectively. Consequently, rapid test of chlorophyll would be available for predicting the nitrogen supplying capacity of tomato.

엽록소 간이검정에 의한 시설재배 토마토의 생산성과 질소공급능력 평가

홍순달,김기인,박효택,강성수 한국토양비료학회 2000 한국토양비료학회 학술발표회 초록집 Vol.2000 No.5

엽록소 검정에 의한 시설재배 토마토의 질소 덧거름 시비량 추정

홍순달,김기인,강성수,임태준 한국토양비료학회 2001 한국토양비료학회 학술발표회 초록집 Vol.2001 No.5

질산태 질소 토양검정에 의한 시설재배 토마토의 질소시비량 결정

홍순달,강성수,김기인 한국토양비료학회 2001 한국토양비료학회 학술발표회 초록집 Vol.2001 No.5

생육중반기 피복제거가 작토층의 수분 및 무기성분 변화와 연초양분흡수에 미치는 영향

홍순달,이윤환,김재정,육창수 한국연초학회 1986 한국연초학회지 Vol.8 No.1

This experiment was carried out to Investigate the environmental changes of rhizosphere, behavior of nutrient components in soul, and nutrients uptake and growth response of the tobacco plant in the condition that mulch as polyethylene film, had been removed on the ridge at the 50th day after transplanting in comparison with continuous mulching condition. The results obtained were as follows; 1. After rainfall, soil moisture content In the plow layer was greatly increased without mulch in comparison with that of the plot with mulch. As a result, leaf water potential of tobacco plant without mulch was higher than that with mulch. 2. Available nutrients such as $NH_4-N, \;NO_3-N$, and total salts in the plow layer of the plot without mulch tended to be Increased, and especially accumulated on the surface layer owing to the redistribution of soil water by rainfall during the latter growth stage after removing mulch. 3. Nutrients uptake by tobacco was much more enhanced in the plot without mulch and resulted in higher contents of total nitrogen, $NO_3-N, \;P_2O_5, \;and K_5O$ in the tobacco leaf Especially higher content of nitrogen caused the delay of maturity resulting In the increased of dry weight of top part of tobacco in the plot without mulch toned to be Increased in comparison with that in mulching condition. Content of total nitrogen, $NO_3-N$, and nicotine in flue-lured leaves was much higher in the plot without. mulch than in mulching condition, but lower content of reducing sugar in the plot without mulch resulted in lower quality of tobacco.

Test Strip Reflectometer에 의한 토양의 질산태 질소 실시간 검정법

홍순달,김기인,강성수,양원석 한국토양비료학회 2001 한국토양비료학회 학술발표회 초록집 Vol.2001 No.5

황색종잎담배의 칼륨결핍증에 관한 연구 (시비수준에 따른 엽위 및 엽부위별 질소와 칼륨함량의 상호관계)

홍순달,이윤환,김재정 한국연초학회 1982 한국연초학회지 Vol.4 No.1

Potassium deficiency symptoms were studied with flue-cured tobacco which was applied with different levels of compound fertilizer (10-15-20) ; 75kg, 100hg, and 125kg/10a. Ratio of N/$K_2O$ in leaves was increased from bottom to top stalk position due to the increase of nitrogen content in leaves. Nitrogen content in leaves was increased from stalk to tip as wall as from midrib to laminae, but vice versa in potassium content. Consequently, resulting in potassium deficiency symptoms in tip of leaves. Rate of reabsorption by rainfall during the latter part of growth was highest at top stalk position in case of nitrogen, but lowest in potassium. This observation was more evident with higher application rate of fertilizer. Nitrogen content of about 4 % in leaves of top stalk position applied with 125kg/10a was maintained up to 85days after trans planting. No increase in potassium in upper leaves was observed over the level of 100kg/10a fertilizer application. As the result, N/$K_2O$ ratio in leaves of top stalk position applied with 125kg/10a was kept at more than 1.0 up to 85days after transplanting, but it was less than 0.9 at 65days after tracts planting with less than 100kg/10a fertilizer application.

인사말

홍순달 한국토양비료학회 2008 한국토양비료학회 학술발표회 초록집 Vol.2008 No.9

채소작물(菜蔬作物)의 시설재배(施設栽培) 상토재료(床土材料)로서 왕겨훈탄(燻炭)의 활용방안(活用方案)에 관(關)한 연구(硏究);산(酸) 종류(種類)에 따른 왕겨훈탄(燻炭)의 중화처리(中和處理) 채소작물(菜蔬作物)의 생장(生長)에 미치는 영향(影響)

홍순달,Hong, Soon-Dal 한국환경농학회 1993 한국환경농학회지 Vol.12 No.3

The growth responses of Chinese Cabbage, lettuce, and spinach were evaluated to investigate the feasibility of carbonized rice-hulls as a sanitary cultivation media instead of soil. The carbonized rice-hulls were pretreated with several inorganic acid such as nitric, sulfuric, hydrochloric, and phosphoric acid for neutralization. The neutralization of the carbonated material using nitric, sulfuric, and phosphoric acid did not alter the growth responses of these vegetables compared to those of control plants grown on the normal cultivation soil. Especially, the nitric acid pretreatment resulted in the better growth of these plants than the mixture of soil and compost. This is probably due to the additional supply of the nitrate-nitrogens. However the growth responses of lettuce and spinach on the carbonated materials neutralized with hydrochloric acid were different and appeared to be having symptom which is analogous to the chloride toxicity during the late stage of growing. Although no difference was observed in chemical composition, the contents of protein-nitrogen were higher in the plants grown on the carbonated material compared to those of plant grown on the mixture of soil and compost. Based on our preliminary results, the carbonized and neutralized rice-hulls are very useful bed material for the sanitary cultivation under the controlled environmental condition.

연초재배기간중(煙草栽培期間中) 증산량(蒸散量) 및 수량(收量) I. 연초생육기간(煙草生育期間)동안의 증산량변화(蒸散量變化)와 토양수분(土壤水分)과의 상호관계(相互關係)

홍순달,김재정,조성진,이윤환,Hong, Soon-Dal,Kim, Jai-Joung,Cho, Seong-Jin,Lee, Yun-Hwan 한국토양비료학회 1989 한국토양비료학회지 Vol.22 No.3

P.V.C관(管)(직경 40cm)을 이용(利用)한 지하수위조절용(地下水位調節用) 폿트재배(栽培)(지하수위(地下水位) : 30cm 및 100cm)로 연초생육기간(煙草生育期間)동안의 일증산양변화(日蒸散量變化)와 자연강우분포조건(自然降雨分布條件)에서 재배포장(栽培圃場)의 근권토양수분변화(根圈土壤水分變化)를 조사(調査)한 결과(結果) 연초식물(煙草植物)의 증산(蒸散)으로 수분소모양(水分消耗量)이 가장 많은 기간(期間)은 이식후(移植後) 40일(日)에서 60일(日)까지로 전생육기간(全生育期間)동안(70일(日)) 증산(蒸散)된 양(量)의 약(約) 50%를 차지했다. 따라서 재배포장(栽培圃場)의 근권토양수분량(根圈土壤水分量)은 이 기간(期間)동안에 매우 낮은 조건(條件)으로 지속되었다. 또 연초식물(煙草植物)의 증산량(蒸散量)은 기상환경변화(氣象環境變化)에 따라 민감하게 영향을 받아 청명한 날은 흐리고 비오는날보다 약(約) 3배(倍) 많았으며 주간(晝間)의 14:00시(時)부터 16시(時)사이에 최대증산량(最大蒸散量)을 보였고 24:00시(時)부터 08:00시(時)사이에 가장 적은 증산량(蒸散量)을 나타냈다. Successive changes of transpiration by the tobacco plant during the growing season with pot trial treated with 30cm and 100cm ground water tables were compared with change of soil moisture content in rhizospere of field under natural rainfall conditions. Transpiration from the 41st to the 60th day after transplanting was the highest showing about 50% of total transpiration of whole cultivation period. As the result, soil moisture condition in rhizospere of field was kept insufficiently during the above period. Transpiration by the plant was so remarkably affected by the meteorological environment that the transpiration on rain-cloudy day was one third of that on clear day at middle stage of the growth. Maximum transpiration in a clear day was obtained from 14:00 to 16:00 at the 58th day after transplanting.